• Journal of the Korean Applied Science and Technology
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• v.33 no.3
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• pp.498-506
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• 2016

1, 2-Hexanediol galactoside (HD-gal) has been previously synthesized from 1, 2-hexanediol (HD), in which recombinant ${\beta}$-galactosidase (${\beta}$-gal) of Escherichia coli (E. coli) was used for transgalactosylation reaction. In this study, a method for HD-gal purification from the reaction mixture was particularly investigated. Using ${\beta}$-gal-containing E. coli, HD-gal was synthesized from 75 mM HD for 48 hr under 300 g/l lactose concentration. Then, HD-gal synthesis from HD was confirmed by TLC analysis, and the existence of E. coli ${\beta}$-gal during 48 hr-reaction was also confirmed by Western blotting, in which the conversion yield of HD to HD-gal reached about 94% during 48 hr. To establish an efficient method for HD-gal purification, we carried out the solvent extraction of the reaction mixture, followed by silica gel chromatography, particularly in order to remove the residual HD. Two water-immiscible solvents, such as methylene chloride and ethyl acetate, were investigated comparatively to find out appropriate solvent. Then, it was found that residual HD was almost removed when ethyl acetate extraction of water phase of reaction mixture was carried out four times. Subsequently, silica gel chromatography was carried out, and purified HD-gal could be finally obtained. The production yield for HD-gal from 75 mM HD was $8.9{\pm}0.6%$ (n=3) (mole basis) or $21.1{\pm}1.4%$ (n=3) (weight basis). For further study, using purified HD-gal, we will investigate the minimum inhibitory concentrations (MICs) of HD-gal against bacteria. In addition, cytotoxicity to human skin cells of HD-gal will be examined.

• Journal of Yeungnam Medical Science
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• v.13 no.2
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• pp.279-294
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• 1996

This study was carried out to develop a Korean language version of Zung's self-rating anxiety scale(SAS) from august, 1994 to September, 1996. The subjects consisted of 205 normal control subjects from the general population group, and 97 subjects with anxiety disorders. These 97 subjects were chosen from a group by the structured clinical interview for DSM-IV of in patients and out patients. Both normal control subjects and anxiety disorder subjects were drawn utilizing a cluster of sampling methods. In order to analyze the data on anxiety scores, Pearson's product moment correlation coefficient method was carried out, as well as reliability, factor analysis and discriminant function analysis, utilizing the SPSS/PC+ program. The results obtained were as follows: The mean average of the total anxiety scores were 32.36 + 6.35 for the normal control subjects and 50.53 + 7.67 for anxiety disorder subjects. Test-retest reliability(coefficient r=0.98, p < 0.001), and internal consistency(coefficient r=0.96, p < 0.001) were satisfactory. Factor analysis using oblique technique rotation yielded four factors. The normal control subjects scored higher concerning the symptoms such as sweating, restlessness, apprehension, insomnia and dyspnea, and lower for faintness, mental disintegration, paresthesia, dizziness and tremor. On the other hand, for the anxiety disorders, apprehension, restlessness, sweating, dyspnea and insomnia scored higher, and lower for faintness, paresthesia, nightmare, dizziness and tremor.

• Journal of the Korean Geographical Society
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• v.35 no.1
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• pp.3-15
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• 2000

산업화와 더불어 심각해지고 있는 수질오염 문제를 해결하기 위해서는 여러 가지 수질관리 방안이 요구된다. 수질오염이 과거에는 국지적이었으나 점차 광범한 지역으로 확장됨에 다라 지속적인 수질 모니터링에 어려움이 따른다. 본 연구에서는 위성영상을 사용한 원격탐사 기법으로 수역의 수질환경 인자를 추출하고자 한다. 사용된 영상은 Landasat TM이며, 연구지역은 한강하류 지역이다. 수질분석 인자는 클로로필-a, 부유물질, 투명도 등을 선정하였으며, 수면분광반사율의 특징 및 수질인자별 처리기법을 개발하는데 목적을 두었다. 분광특성 분석결과를 요약하면, 첫 번째 스펙트럼 반사율 분석결과 클로로필-a의 농도는 0.4~0.5$\mu\textrm{m}$ 파장대역에서 낮은 반사치 경향을 보이며, 녹색파장대인 0.57$\mu\textrm{m}$ 부근에서 반사율이 높아진다. 두 번째 부유물질의 반사도는 농도가 증가할수록 0.8$\mu\textrm{m}$ 부근에서 상대적으로 낮은 반사율이 나타난다. 마지막으로 투명도가 낮은 수면은 0.55$\mu\textrm{m}$에서 높은 반사율 경향을 보인다. Landsat TM영상을 이용하여 주성분분석 및 비연산처리를 실시하여 수질분석을 시도한 결과를 보면 클로로필-a와 투명도는 제1주성분 영상 및 제2주성분 영상에서 현장 실측자료와 유사한 결과를 얻을 수 있었으며, 부유물질은 밴드 2와 밴드 4의 비연산처리를 통하여 분포도를 작성할 수 있었다. 이상의 결과들은 계절적 및 시간적 변화에 따라 파장대역이 달라질 수 있다. 그러므로 위성자료를 이용하여 보다 정확한 수질환경 인자를 추출하기 위해서는 현장실측 및 수역의 분광반사 특성을 지속적으로 조사하여야 한다.때문으로 경주 산사태와 포함-구릉포간 국도면의 산사태가 이 종류의 산사태에 속한다.열 인식의 신뢰도를 향상시킬수 있는 방법을 제안하였다.작성하여 최신 의료영상 처리 기법을 쉽게 임상에 적용하고 실험할 수 있는 장점이 있다. 지대에서 가능하였고, 파종기는 중생종보다 이르게 나타났다. 등숙만한출수기 기준의 안전작기는 조생종과 중생종은 태백고냉지대와 태백준고냉지대, 소백산간지대 일부지역을 제외한 다른 지역에서 설정되었고, 중만생종은 태백고냉지대, 태백준고냉지대, 동해안북부지대, 소백산간지대, 노령소백산간지대의 일부 지역은 벼 담수직파가 불가능하게 판단되었다. information on the regular basis of time and provide it when the users query over the Web-database gateway. The other approach is a shopping agent mechanism, which stores information on "how to shop" and the shopping agent collects the information of product items just after users query about the product and provide the information in real time or notify them by alerting service. Thirty nine shopping information services are compared and classified in this paper and they are extracted from "Naver" and "Yahoo! Korea". The final result shows that most services are just a

• 한국HCI학회:학술대회논문집
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• 2007.02b
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• pp.651-655
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• 2007

카툰 렌더링 그래픽을 기반으로 한 온라인 RPG 게임인 마비노기에서는 게임이용자들이 염색 시스템을 통하여 의복아이템에 자신들이 원하는 색을 자유롭게 표현할 수 있다. 마비노기에서 색에 관련된 주목할 만한 현상은유행색이 존재하고 색에 따라 같은 아이템의 가격이 달라진다는 점이다. 유행색이 존재하는 것은 현실과 비슷하지만 같은 아이템이 색에 따라 가격이 달라지는 것은 현실에서는 보기 드물다. 이는 현실과 비슷하면서도 심리적인 속성이 다른 사이버공간만의 특징이라고 생각해 볼 수 있다. 따라서 마비노기에서 게임이용자들이 색을 선택하고 사용할 때 작용하는 심리기제가 존재할 것이라고 가정해 볼 수 있다. 이에 본 연구에서는 마비노기에서 색 선택 행동의 심리적 유형을 확인하고 유형별 특징을 알아보기 위해 자아존중감을 측정하였다. 예비 조사를 통해 게임이용자 20명과 온라인 인터뷰를 실시하여 색 사용 경험을 추출하고, 추출한 경험을 바탕으로 18문항의 색 선택 행동 문항을 구성하였다. 본 조사에서는 온라인 설문으로 129명의 게임이용자를 대상으로 색 선택 행동과 자아존중감을 측정하였다. 연구 결과 색 선택 행동은 4개의 요인-색채 둔감성, 유행추종, 개성표현, 캐릭터이미지와의 적합성-으로 구분되었다. 색선택 행동 4개의 하위 요인을 기준으로 군집분석한 결과 색 선택 행동 유형은 각각 색 분화형, 유행추종형, 색 둔감형으로 구분되었다. 색 선택 행동 유형과 자아존중감과의 관계에서는 자아존중감 하위 범주 중 부정적인 자기평가, 타인의 의견에 대한 걱정, 의존성의 세 가지 하위 범주에서 색 선택 행동 유형에 따른 차이가 유의미하게 나타났다. 한편 자의식 차원에서는 색 선택 행동 유형간 유의미한 차이가 발견되지 않았다. 유행추종형은 다른 두 유형에 비해 상대적으로 자아존중감 수준이 낮음을 확인할 수 있었다. 반대로 색 둔감형은 다른 두 유형에 비해 상대적으로 자아 존중감 수준이 높았다. 색 분화형은 유행추종형과 색 둔감형의 중간 수준의 자아존중감을 볼 수 있었다. 유행추종형은 자기의 외부에서, 색 둔감형은 자기의 내부에서 색과 관련된 자신에 대한 가치감의 소재와 근원을 찾는 특성이 있다고 해석할 수 있다. 본 연구는 온라인 RPG 게임에서 게임이용자들의 색 선택의 심리적 기제를 확인하고 색 선택 유형별로 자아존중감과의 관계를 확인함으로써 색 선택 행동의 심리적 기제를 밝히려는 기초연구라는 점에 의의가 있다. 추후에는 게임이용자들의 색 선택 행동을 보다 포괄적으로 이해하기 위해 게임이용자들이 가지고 있는 공통적인 색이미지와 개별적인 색이미지 및 색이미지의 심리적 구조를 알아보기 위한 색채감성연구가 필요할 것이다. 이러한 연구들은 게임을 제작할 때 캐릭터 디자인과 아이템 디자인에 어떠한 색을 사용해야 되는지 방향을 설정하는데 도움을 줄 수 있을 것이다.

• Korean Journal of Food Science and Technology
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• v.45 no.6
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• pp.777-784
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• 2013

The ethyl acetate (EtOAc) layer of Canavalia gladiata (sword bean) methanol extracts showed higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity than other layers. Four phenolic compounds were isolated from the EtOAc layer by silica gel column chromatography and prep-HPLC using a guided DPPH radical-scavenging assay. The isolated compounds were identified as methyl gallate (1), gallic acid (2), 1,6-di-O-galloyl ${\beta}$-$\small{D}$-glucopyranoside (3), and 1,4,6-tri-O-galloyl ${\beta}$-$\small{D}$-glucopyranoside (4) based on MS and NMR analyses. Among the four compounds, no. 4 was isolated from this plant for the first time. Their DPPH radical-scavenging activities based on $SC_{50}$ decreased in the following order: 4 (6.9 ${\mu}M$)>3 (8.3 ${\mu}M$)>2 (10.0 ${\mu}M$)>1 (10.3 ${\mu}M$).

• Journal of Soil and Groundwater Environment
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• v.12 no.6
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• pp.100-106
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• 2007

The objective of this study was to evaluate the efficiency of $(NH_4)_2HPO_4$, $Na_2HPO_4{\cdot}12H_2O$, $CaHPO_4{\cdot}2H_2O$, $Ca(H_2PO_4)_2{\cdot}H_2O$ and $H_3PO_4$ for the stabilization of soils contaminated with multi-metals containing Pb, Cd and As. The application rate of stabilizers to soils was determined based on $PO_4/Pb_{total}$ molar ratio of 0.5, 1, 2, 4. The results of Korea Standard Test and TCLP (EPA Method 1311) showed the reduction of metal leachabilities below the regulatory limits for Pb and Cd when $H_3PO_4$ and $Ca(H_2PO_4)_2{\cdot}H_2O$ were applied. However, stabilization efficiency for Cd was low and in case of As leaching concentration increased rather. It is considered that $PO_4$ reacted effectively $Pb^{2+}$ due to leaching Pb under low pH condition created by adding $H_3PO_4$. Accordingly Pb was stabilized by dissolution and precipitation of hydroxypyromorphite. From the change of metals fraction using sequential extraction procedure when $H_3PO_4$ applied as a stabilizer, we confirmed that residual fraction increased more than 60% and this result was accorded with XRD analysis that detected only hydroxypyromorphite peak in $H_3PO_4$.

• The Korean Journal of Food And Nutrition
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• v.26 no.3
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• pp.383-390
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• 2013

Mistlero C was shown to be non-genotoxic in a series of genotoxicity tests, including a bacterial reverse mutation test and a combined in vivo mammalian erythrocyte micronucleus test. In a bacterial reverse mutation assay, no significant increases in the number of revertant colonies, compared to the negative control, was detected in $5,000{\mu}g/plate$ of Mistlero C. In addition, with Mistlero C, no changes were shown in the number of micronucleated polychromatic erythrocytes (MNPCE) among 2,000 polychromatic erythrocytes compared to the negative control. Mistlero C was administered orally in rats to investigate acute toxicity. The $LD_{50}$ values in rats were above 2,000 mg/kg. In a repeated dose, 13-week, oral toxicity study conducted in rats, no compound-related adverse effects were shown at doses of Mistlero C of up to 1,000 mg/kg body weight/day. The results of these studies support the safe use of Mistlero C in food for human consumption.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2016.05a
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• pp.490-492
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• 2016

Pancreatic ultrasound imaging is used to diagnose pancreatic hyperplasia, pancreatic steatosis, pancreatic cancer and the like. If the diagnosis of pancreatic steatosis is pancreatic parenchyma echo shades splashes spleen than in the pancreas ultrasound it determines that the fat is deposited. And research on ultrasound imaging of pancreatic cancer but is actively conducted research studies on pancreatic steatosis is insufficient In addition, pancreatic steatosis is often an error in accordance with the diagnostic criteria are vague and subjective diagnosis of the artisan. This study was a quantitative analysis using the feature value extracting a feature of an image extracted by applying a parameter to the algorithm GLCM image of the normal and pancreatic fat. Setting a region of interest ($5{\times}5pixel$) in the mild 89 case, moderate 89 case, severe 89 case, total image 267 case using GLCM algorithm, and using the Autocorrelation, Sum average, Sum of squares, Sum varience 4 kinds parameter in each image It was analyzed.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.36 no.1
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• pp.97-108
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• 2018

The purpose of this study is to propose the construction of a visibility analysis model, which is the basis of the analysis for landscape management on the heritage sites such as historic villages and scenic sites. Results of the visibility analysis using DEM and the visibility analysis of DSM based on 3D mapping data are compared as follows: Precision level of the extracted data was confirmed to be less than 6.5cm, based on RTK survey results produced by constructing orthoimage data and DSM from the digital data of 2cm-class GSD(Ground Sample Distance) obtained by using a small UAV(Unmanned Aerial Vehicle). As a result of comparing the visibility analysis data of Digital Surface Model (DSM) using a small UAV with Digital Elevation Model(DEM) applying the height of the building to the Digital Topographic Map, it was confirmed that more realistic visibility analysis can be accomplished by applying DSM, as the structures such as fences, trees, and houses are reflected in the topographic data. The visibility analysis model using the 3D mapping technique can efficiently obtain the constantly changing topographic information when needed, by immediately constructing the data by utilizing a small UAV. It seems to be possible to propose a reasonable analysis result for preservation management such as landscape evaluation of cultural property.

• Current Research on Agriculture and Life Sciences
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• v.31 no.3
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• pp.200-208
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• 2013

The purposes of this study are developing a stress index for each individual major trail in the national parks and contribute to establishing a park management policy based on scientific analyses using the index. We collected data from 144 sections of trails in national parks, conducted the Delphi survey and factor analysis, and generated the stress index by standardizing scores of factors contributing to the stress for each trail. As aresult, total eight factors contributing to the stress were considered and four of them, i.e. the amount of visits, the effect of visits, the degree of trail destruction, and species diversity, were selected to generate the stress index. How to weigh factors was determined by their importance obtained in the second Delphi survey and the average from a 5 point Likert scale. Then, the cumulative probabilities from the standardized scores were graded into five groups. In conclusion, there were twenty trails from the six national parks including Jirisan, Bukhansan, Deogyusan, Naejangsan, Seoraksan in the first grade which gained over 80 as the stress index.