• Korean Journal of Environment and Ecology
• /
• v.14 no.3
• /
• pp.205-211
• /
• 2000

본연구는 조경소재로 이용가능성이 크지만 현재 자생지가 파괴되어 복원이 요구되고 있는 야생자란의 대량번식을 위해 무균배양시 배지 내 담함량의 변화와 펩톤, 트립톤 등의 첨가가 종자발아와 계대배양 후 유식물의 생육에 미치는 영향을 알아보고자 실시하였다. 배지 내 펩톤과 트립톤의 첨가는 발아에 영향을 주지는 않았지만, 당의 함량은 그 농도가 10g/L까지 증가함에 따라 발아율을 높였다. 또한 발아 후 유식물의 생육시 당의 첨가는 뿌리의 생육을 두드러지게 향상시켰으며, 생체중도 거의 2~3배정도 많았다. 하이포넥스 배지(대조구)에서는 높은 발아율을 보였지만 유식물의 생육은 트립톤 첨가배지(2g/L)에서 많았는데 엽수, 뿌리수, 엽장 근장, 생체중 등이 모두 다른 처리구의 2~3배에 이르는 초기생육을 보였다. 계대배양 이후의 생육상은 펩톤 첨가배지에서 가장 많은 생육량을 보였는데 특히 엽장과 엽폭 그리고 근장이 다른 처리구보다 월등히 높은 경향을 나타냈다. 생체중도 한 개체당 0.18g으로 가장 높게 나타나 펩톤의 첨가가 계대배양 이후의 생육을 크게 촉지시키는 것으로 나타났다. 결론적으로 하이포넥스 배지에 트립톤 2g/L를 첨가하였을 때 발아율과 유식물의 생육이 다른 배지에 대해 매우 양호한 것으로 나타나 자생 자란의 종자발아용 배지로 가장 적당한 것으로 사료된다. 그리고 이후 계대배양시에는 펩톤의 첨가 (3g/L)가 유식물의 생육을 가장 촉진시키는 것으로 나타났다.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.35 no.1
• /
• pp.65-72
• /
• 2009

Skin aging appears to be principally attributed to a decrease in both levels of Type I collagen and regeneration ability of dermal fibroblasts. It is important to introduce an efficient and safe agent for effective management of skin aging. To this end, we performed screening for anti-ageing agents and then found that vegetable peptones (pea and wheat) promoted cell proliferation of adult stem cells. Vegetable peptones may be considered as useful medium additives because it can supply nutrients, peptides, amino acids or growth factor analogues. This study was designed to investigate effects of vegetable peptones on cell proliferation/collagen production and their possible mechanisms in human dermal fibroblasts. In cell proliferation assay, vegetable peptones significantly promoted cell proliferation in a concentration-dependent manner. In addition, human COL1A2 promoter luciferase and type I procollagen synthesis assays showed that vegetable peptones induce type I procollagen production through the activation of COLlA2 promoter. In both TGF-${\beta}1$ luciferase reporter and ELISA assays, vegetable peptones was found to induce TGF-${\beta}1$ production, suggesting that vegetable peptones induce type I procollagen production through the activation of TGF-${\beta}1$. When applied topically in a human skin twice a day for an 4-week period of time, vegetable peptones did not induce any adverse reactions. Theretore, based on these results, we suggest the possibility that vegetable peptones may be considered as an attractive, wrinkle-reducing candidate for topical application.

• 한국생물공학회:학술대회논문집
• /
• 2005.10a
• /
• pp.250-255
• /
• 2005

Trichoderma inhamatum KSJ1, a filamentous fungus, isolated from rotten wood showed high ability to hydrolysis of cellulosic materials. Enzyme productivity by strain KSJ1 was high in the cultivation using carbon sources such as cellulosic materials and lignocellulosic wastes as rice straw and paper waste. In previous study peptone was one of optimum organic nitrogen sources in producing cellulases for saccharification of food wastes. However, it was too expensive using peptone as organic nitrogen source, so, in this study, soybean and yeast were applicated to substitute peptone. Yeast showed producing high enzyme activity, so it was estimated that yeast is available in producing cellulase using Trichoderma inhamatum KSJ1 at industrial Production.

• Journal of Mushroom
• /
• v.18 no.4
• /
• pp.365-371
• /
• 2020

In this study, we analyzed the effect of media on the production of carotenoids and mycelia by using Perenniporia fraxinea. Malt extract-based medium with less than 0.1% peptone stimulated the production of carotenoids, and the one with more than 0.2% peptone inhibited its production. P. fraxinea grown in medium without malt extract did not produce carotenoids, although a small amount of peptone was added to the medium.After carotenoid production, the culture broth was separated using simple centrifugation and the supernatant was harvested as a carotenoid solution. Ethanol was used to extract carotenoids from mycelia. Carotenoid solution separated or extracted from the culture solution showed DPPH radical scavenging activity. The antioxidant carotenoids produced by P. fraxinea are derived from natural products, have no toxicity and side effects, and exhibit excellent antioxidant effects; therefore, they can be effectively used to remove oxides produced by active oxygen.

• Journal of Mushroom
• /
• v.10 no.2
• /
• pp.74-82
• /
• 2012

Sixty strains of Pleurotus ostreatus, white-rot fungi, were screened for production ability of their lignocellulolytic enzymes to selectively wood degradation. That results were shown that all of screened strains were produced lignocellulolytic enzymes on 2nd screening liquid culture medium. However, cellulase activity of selected six strains of P. ostreatus was low in avicel-yeast-peptone liquid culture medium. In the case of xylan degrading enzyme, No. 6 and No. 38 strains produced a xylanase(above 1.0U/ml) and a 1,4-${\beta}$-xylosidase (above 0.15 U/ml). Examination of the ligninolytic enzyme profiles of selected thirteen strains of the P. ostreatus, in the presence of Remazol Brilliant Blue R(RBBR), were observed that laccase(Lac) activity were earlier reached maximum level(0.8-2.0 U/ml) and then Mn-dependent peroxidase (MnP) were reached maximum level(0.5-1.5 U/ml) in glucose-yeast-peptone(GYP) medium. On the other hand, activity of lignin peroxidase(LiP) was not detected in this medium. I selected the No. 42 strain of P. ostreatus produced high levels of Mn-dependent peroxidase and laccase based on the screening method.

• Journal of the Korean Wood Science and Technology
• /
• v.28 no.1
• /
• pp.48-54
• /
• 2000

The effects of thiamin on vegetative mycelial growth and fruit body formation of Lentinuia edodes were investigated in basal peptone-glucose liquid medium in relation to the uptake of thiamin. Thiamin was essential for fruit body formation, and the minimum requirements for thiamin were estimated to be approximately 10 ${\mu}g$/L. The vegetative mycelial growth was little influenced by the addition of thiamin in the range of 1.5 ${\mu}g$~1.5 mg/L. While the mycelium was successively transferred to fresh peptone-glucose-agar medium three times, the repression of mycelial growth was not significant. Even in cases using vitamin-free casamino acid or glutamic acid as a nitrogen source instead of peptone, a thiamin deficiency for mycelial growth did not occur as a result of transferring the mycelia to fresh media. Almost all of the thiamin contained in the media accumulated in the mycelia during the first 3 weeks of a 9-week incubation. These results suggest that only trace amounts of thiamin are required for vegetative mycelial growth in Lentinula edodes and that almost all thiamin added to a basal medium will be used for fruit body formation.

• Korean Journal of Food Science and Technology
• /
• v.13 no.3
• /
• pp.233-240
• /
• 1981

Nutritional characteristics of mycelial growth and fruit-body formation of Flammulina velutipes in synthetic media were investigated. The results are summarized as follows: 1. Among sugar substances, mannitol gave rapid mycelial growth and formation of fruit-body with higher yield, and among organic acids only succinic acid showed the fruit-body but poorly. Ethyl alcohol and glycerol comparatively promoted the formation of the fruit-body with higher yield. 2. Among nitrogen sources, peptone resulted in rapid mycelial growth and fruit-body formation with higher yield. and among amino acids glycine gave fast fruit-body formation with higher yield. However. nitrite nitrogen, lysine and methionine showed no mycelial growth at all. 3. The concentration of substances tended to affect the fruit-body formation and yield. Lower concentration of mannitol and peptone prompted fruit-body formation but with low yields, and higher concentration delayed the fruit-body formation with increased yields.

• Journal of Food Hygiene and Safety
• /
• v.28 no.3
• /
• pp.279-285
• /
• 2013

To establish sample preparation method for detection of food-borne pathogens from lettuce, perilla leaves, cucumber, pepper, and cherry tomato, the influences of diluent composition, processing time, and proportion of diluent to sample were examined. Each produce was inoculated with 6.0 log $CFU/cm^2$ of Escherichia coli O157:H7, Salmonella Typhimurium, Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus. Each produce was treated with 0.1% peptone water, and D/E neutralizing broth. Processing time of produce was 30, 60, 90, and 120s, and the proportion of diluent to sample was 2 : 1, 4 : 1, 9 : 1, and 19 : 1. The number of bacteria after treatment of D/E neutralizing broth was higher than that of 0.1% peptone water (P<0.05). In cherry tomato, the population of S. typhimurium recovered from treated with D/E broth was higher than that recovered from treated with 0.1% peptone water by 1.05 log $CFU/cm^2$ (P<0.05). No difference in numbers of pathogens was observed in processing time. Optimum proportion of diluent to perilla leaf, iceberg lettuce, cucumber, green pepper, and tomato was 9 : 1, 4 : 1, 2 : 1, 2 : 1, and 2 : 1, respectively. These data suggest that D/E neutralizing broth should be recommend as diluent, and the diluent volume applied to produce should be determined in proportion to produce surface area per weight (g).

• Korean Journal of Microbiology
• /
• v.47 no.3
• /
• pp.249-254
• /
• 2011

Mycophenolic acid blocking the synthesis of xanthosine monophosphate is a nonnucleoside inhibitor of inosine monophosphate dehydrogenase. Therefore mycopholoic acid is a drug currently used as immunosuppressive agent in transplantation of heart, kidney and liver. Mycophenolic acid has been industrially produced through fermentation process by fungus Penicillium brevi-compactum. In this study, the profile of mycophenolic acid fermentation was observed in 5L-jar fermentor to investigate the utilization of carbon and nitrogen sources and the production of mycophenolic acid. It was investigated that what kind of carbon sources was better to cell growth and mycophenolic acid production. Fructose was the best carbon source for mycophenolic acid fermentation, but it is the most expensive one. Thereafter molasses containing sucrose as the supply source of fructose was confirmed to be the best carbon source for the industrial production. Use of molasses increased the fermentation yield of mycophenolic acid more than two times higher than glucose. It was confirmed that urea was the best inorganic nitrogen source, which did not give rise to sudden drop of culture pH. Addition of urea increased the fermentation yield of mycophenolic acid about 3.6 times higher than addition of ammonium nitrate as control. Casein, peptone and casamino acid originated from milk protein increased the fermentation yield of mycophenolic acid about 3.4 times higher than control. Peptone and casamino acid, which are casein hydrolysates, increased cell growth considerably as well.

• Korean Journal of Food Science and Technology
• /
• v.13 no.1
• /
• pp.43-52
• /
• 1981

A series of thermal destruction studies of the most heat resistant yeast strain No. 15 among 61 isolates were conducted in order to establish the optimum pasteurization condition of peach solid pack. The results obtained are summarized as follows: 1. A survival curve of the selected yeast strain No. 15 at $58^{\circ}C$ consisted of heat labile and heat stable fraction, showing broken curve. 2. The actively growing cell showed less recovery rate than 10 day rested cell after agitation-growing for 90 hr. For heating menstrua, peptone solution gave higher recovery rate than peach juice. For recovery medium, YM agar gave higher recovery rate than peach juice agar. The selected yeast was more resistant to heat at pH 4.0 than at pH 3.5 in both heating menstrua and recovery medium. 3. Z value of TDT curve of the selected yeast (heating : at pH 3.5 in peach juice. recovery: at pH 3.5 in peach juice agar) was $4.8^{\circ}C$. 4. The selected yeast No. 15 was identified as Torulopsis candida. 5. In the inoculated pack test of 4 oz can, it was concluded that the optimum P.U. 70/5 was 168 (center temp; $78.5^{\circ}C$, initial temp; $18^{\circ}C$, processing time; 18 min, initial yeast count; $1.0{\times}10^7$ per can).