Browse > Article

Effects of Vegetable Peptones on Promotion of Cell Proliferation and Collagen Production  

Jung, Eun-Sun (Biospectrum Life Science Institute)
Lee, Jong-Sung (Biospectrum Life Science Institute)
Lee, Jienny (Biospectrum Life Science Institute)
Huh, Sung-Ran (Biospectrum Life Science Institute)
Kim, Young-Soo (Biospectrum Life Science Institute)
Hwang, Wang-Taek (Biospectrum Life Science Institute)
Park, Deok-Hoon (Biospectrum Life Science Institute)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.35, no.1, 2009 , pp. 65-72 More about this Journal
Abstract
Skin aging appears to be principally attributed to a decrease in both levels of Type I collagen and regeneration ability of dermal fibroblasts. It is important to introduce an efficient and safe agent for effective management of skin aging. To this end, we performed screening for anti-ageing agents and then found that vegetable peptones (pea and wheat) promoted cell proliferation of adult stem cells. Vegetable peptones may be considered as useful medium additives because it can supply nutrients, peptides, amino acids or growth factor analogues. This study was designed to investigate effects of vegetable peptones on cell proliferation/collagen production and their possible mechanisms in human dermal fibroblasts. In cell proliferation assay, vegetable peptones significantly promoted cell proliferation in a concentration-dependent manner. In addition, human COL1A2 promoter luciferase and type I procollagen synthesis assays showed that vegetable peptones induce type I procollagen production through the activation of COLlA2 promoter. In both TGF-${\beta}1$ luciferase reporter and ELISA assays, vegetable peptones was found to induce TGF-${\beta}1$ production, suggesting that vegetable peptones induce type I procollagen production through the activation of TGF-${\beta}1$. When applied topically in a human skin twice a day for an 4-week period of time, vegetable peptones did not induce any adverse reactions. Theretore, based on these results, we suggest the possibility that vegetable peptones may be considered as an attractive, wrinkle-reducing candidate for topical application.
Keywords
cell proliferatin; type I procollagen; COL1A2 promoter; vegetable peptones; irritation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 C. E. Griffiths, The clinical identification and quantification of photodamage, Br. J. Dermatol., 127, 37, (1992)   PUBMED
2 R. E. Watson, C. E. Griffiths. N. M. Craven, C. A , Shuttleworth, and C. M. Kielty, Fibrillin-rich microfibrils and reduced in photoaged skin: distribution at the dermal-epidermal junction, J. Invest. Dermatol., 112, 782 (1999)   DOI   ScienceOn
3 J. Varani, R. L. Warner, M. Gharaee-Kermani, S. H. Phan, S. Kang, J. H. Chung, Z. Q. Wang, S. C. Datta, G. J. Fisher, and J. J. Voorhees, Vitamin A antagonizes decreased cell growth and elevated collagen-degrading matrix metalloproteinases and stimulates collagen accumulation in naturally aged human skin, J. Invest. Dermatol., 114, 480 (2000)   DOI   ScienceOn
4 J. H. Chung, J. Y. S대, H. R. Choi, M. K. Lee, C. S. Youn, G. Rhie, K. H. Cho, K. H. Kim, K. C. Par, and H. C. Eun, Modulation of skin collagen metabolism in aged and photoaged human skin in vivo, J. Invest. Dermatol., 117, 1218 (2001)   DOI   ScienceOn
5 D. C-H. Jan, S. J. Jones, A. N. Emery, and M. Al-Rubeai, Peptone, a low-cost growth-promoting nutrient for intensive animal cell culture, Cytotechnology, 16, 17 (1994)   DOI   ScienceOn
6 J. S. Lee, J. Lee, H. Hwang, E. S. Jung, S. R. Huh, and D. H. Park, Promotion of stem cell proliferation by vegetable peptone, Cell Proliferation, in press (2009)   DOI   ScienceOn
7 J. S. Lee, E. S. Jung, Y. J. Kim, J. H. Park, J. I. Park, S. T. Hong, J. E. Kim, C. G. Hyun, Y. S. Kim, and D. H. Park, Asiaticoside induces human collagen I synthesis through TGF$\beta$ receptor I kinase (T$\beta$RI kinase)-independent Smad signaling, Planta. Med., 72(4), 324, (2006)   DOI   ScienceOn
8 S. R. Huh, J. S. Lee, E. S. Jung, S. C. Kim, J. I. Kang, J. Lee, Y. W. Kim, Y. K. Sung, H. K. Kang, and D. H. Park, A cell-based system for screening hair growth-promoting agents, Arch. Dermatol. Res., in press (2009)
9 H. S. Talwar, C. E. Griffiths, G. J. Fisher, and T. A. Hamilton, Reduced type I and type III procollagens in photodamaged adult human skin, J. Invest. Dermatol., 105, 285, (1995)   DOI   PUBMED   ScienceOn
10 F. O. Franek, O. Hohenwarter, and H. Katinger, Plant protein hydrolysates: preparation of defined peptide fractions promoting growth and production in animal cells culture, Biotechnol. Prog., 16, 688 (2000)   DOI   ScienceOn
11 M. D. Pierson, S. L. Payne, and G. L. Ades, Heat injury and recovery of vegetative cells of Clostridium botulinum type E. Appl. Microbiol., 27(2), 425 (1974)   PUBMED   ScienceOn
12 V. L. Chen, R. Fleischmajer, E. Schwartz, M. Palaia, and R. Timpl, Immunohistochemistry of elastotic material in sun damaged skin, J. Invest. Dermatol., 87, 334 (1986)   DOI   ScienceOn
13 S. R. Huh, J. S. Lee, E. S. Jung, Y. G. Ham, S. S. Kim, C. G. Hyun, Y. S. Kim, and D. H. Park, A Cell-based assay system for high-throughput screening of anti-wrinkle agents in human dermal fibroblast transfectant cells, Biotechnol. Appl. Biochem, 47(1), 27 (2007)   DOI   ScienceOn
14 R. Warren, V. Gartstein, A, M. Kligman, W. Montagna, H. A. Allemdorf, and G. M. Ridder, Age, sunlight and facial skin: a histological and quantitative study, J. Am Acad. Dermatol., 25, 751 (1991)   DOI   ScienceOn
15 M. Brennan, H. Bhatti, K. C. Nerusu, N. Bhagavathula, S. Kang, G. J. Fisher, J. Varani, and J. J. Voorhees, Matrix metalloproteinase-1 is the major collagenolytic enzyme responsible for collagen damage in UV-irradiated human skin, Photochem. Photobiol., 78, 43 (2003)   DOI   ScienceOn
16 J. G. Smith. E. A. Davidson. W. M. Sams, and R. D. Clark, Alterations in human dermal connective tissue with age and chronic sun exposure, J. Invest. Dermatol., 39, 347 (1962)   DOI   ScienceOn
17 H. P. Bonarius, V. Hatzimanikatis, K. P. Meesters, C. D. de Gooijer, G. Schmid, and J. Tramper, Metabolic flux analysis of hybridoma cells in different culture media using mass balances, Biotechnol Bioeng., 50(3), 299 (1996)   DOI   PUBMED
18 C. E. Griffiths, A. N. Hussman. G.Majmudar, G. R. S. Singer, and T. A. Hamilton, Restoration of collagen formation in photodamaged skin by tretinoin (retinoic acid), N. Eng. J. Med, 329, 530 (1993)   DOI   ScienceOn
19 K Kadoya, T Sasaki, G. Kostka, R Timpl, K :Vlatsuzaki, N. Kumagai, L Y. Sakai, T. Nishiyama, and S. Amano, Fibulin-5 deposition in human skin: decrease with ageing and ultraviolet B exposure and increase in solar elastosis, Br. J. Dermatol., 153, 607 (2005)   DOI   ScienceOn
20 R. Heidemann, C. Zhang, H. Qi, R. J. Larrick, C. Rozales, S. Park, S. Chuppa, M. Ray, J. Michaels, K. Konstantinov, and D. Naveh, The use of peptones as medium additives for the production of a recombinant therapeutic protein in high density perfusion cultures of mammalian cells, Cytotechnology, 32(2), 157 (2000)   DOI   ScienceOn