• Title/Summary/Keyword: 펙틴

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Chemical Property and Macrophage Stimulating Activity of Polysaccharides isolated from Brown Rice and Persimmon Vinegars (현미식초 및 감식초 유래 다당류의 대식세포 자극활성 및 화학적 특성)

  • Kim, Dong-Su;Shin, Kwang-Soon
    • The Korean Journal of Food And Nutrition
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    • v.27 no.6
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    • pp.1033-1042
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    • 2014
  • To elucidate the novel biological function of Korean traditional vinegars, crude polysaccharides were isolated from vinegars manufactured at home and abroad, and their chemical properties and immuno-stimulating activities were examined. Three kinds of polysaccharides from Korean brown rice vinegar (KBV-0), Japanese brown rice vinegar (JBV-0) and Korean persimmon vinegar (KPV-0) showed higher immuno-stimulating activity. Component sugar analysis indicated that KBV-0 and JBV-0 mainly consisted of mannan, whereas KPV-0 existed as pectic materials. Three polysaccharides did not show any cytotoxicity to RAW 264.7 cell, whereas RAW 264.7 cells stimulated with KBV-0, JBV-0 and KPV-0 showed enhanced production of various cytokines such as IL-6, IL-12 and TNF-${\alpha}$ in dose-dependent manners. However, the activity of KPV-0 was more potent than that of KBV-0 and JBV-0. Also, only KPV-0 augmented FcR II expression related with phagocytosis of macrophages. The results suggest among the tested vinegars, that the Korean persimmon vinegar has the most potent immune-stimulating activity, and it could possibly serve as industrial applications as functional materials.

Histochemical and Cytochemical Studies on the Secondary Xylem Formation during Grafting Process of Pinus thunbergii (접목 과정에서 형성된 신생 2차목부의 조직학적, 세포화학적 연구)

  • Kim, Jong Sik;Lee, Kwang Ho;Kim, Yoon Soo
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.6
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    • pp.1-11
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    • 2006
  • Grafted tissues were investigated using various microscopic techniques. Pinus thunbergii was used as stock and scion and autografted by cleft graft method. Histochemically, grafting processes can be proceeded by four stages: 1) formation of necrotic layer, 2) proliferation of callus, 3) development of neo-cambium from callus, and 4) restoration of new vascular xylem. Necrotic la yer composed of pectin and lignin was gradually degraded during grafting process and disappeared when new union was formed between stock and scion. A large number of starch and lipid bodies in the cytoplasm were also gradually degraded during grafting process and disappeared at the grafting interface. Nucleus and plasmodesmata were not changed. Bubble-like callus was generated from all living parenchyma cells and from the callus. The tracheary elements differentiated from the callus had either reticulate or pit-like thickenings in the secondary walls with bordered pits. Secondary cell wall thickening occurred toward filing to the void parts between reticulated secondary wall. Tracheids formed in the secondary xylem were short with irregular wall thickness. New secondary xylem cells with swirled shapes, which developed in graft union were oriented horizontally and obliquely to axis of the stem.

Thermotropic Liquid Crystalline Properties of (8-Cholesteryloxycarbonyl)heptanoated Polysaccharides ((8-콜레스테릴옥시카보닐)헵타노화 다당류들의 열방성 액정 특성)

  • Jeong Seung-Yong;Ma Yung-Dae
    • Polymer(Korea)
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    • v.30 no.4
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    • pp.338-349
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    • 2006
  • Fully or nearly fully(8-cholesteryloxycarbonyl)heptanoated polysaccharide derivatives were synthesized by reacting cellulose, amylose, chitosan, chitin, alginic acid, pullulan or amylopectin with (8-cholesteryloxycarbonyl)heptanoyl chloride (CH8C), and their thermotropic liquid crystalline behaviors were investigated. Like in the case of CH8C, all the polysaccharide derivatives formed monotropic cholesteric phases with left-handed helicoidal structures whose optical pitches $({\lambda_m}'s)$ decrease with increasing temperature. Amylopectin derivative also formed a monotropic cholesteric phase with lefthanded helicoidal structures but, in contrast with the other derivatives, did not display reflection colors over the full cholesteric range, suggesting that the helicoidal twisting power of the cholesteryl group highly depends on the branched structure in amylopectin. The thermal stability and degree of order in the mesophase, the magnitude of ${\lambda}_m$ at the same temperature, and the temperature dependence of the ${\lambda}_m$ observed for polysaccharide derivatives were entirely different from those reported for the polymers in which the cholesteryl groups are attached to flexible or semiflexible backbones through flexible spacers. The results were discussed in terms of the difference in the chemical structures of the main and side chains and flexibility of the main chain.

The Effect of Pear Pectin on Blood Pressure, Plasma Renin ANP and Cardiac Hypertrophy in Hypertensive Rat Induced by 2K1C (배 추출 펙틴이 2K1C 고혈압 흰쥐의 혈압, 혈장 Renin, ANP 및 Cardiac Hypertrophy 에 미치는 영향)

  • Na, Chang-Su;Yun, Dae-Hwan;Choi, Dong-Hee;Kim, Jeong-Sang;Cao, Chun-Hua;Eun, Jong-Bang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.5
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    • pp.700-705
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    • 2003
  • Oriental pear was used as treatment of asthma, control of blood pressure tonic medicine vasoactio, diabetes in oriental medicine. In this study, it was investigated that pear pectin effects on cardiovascular system as blood pressure and renin and atrial natriuretic peptide (ANP) in plasma, cardiac hypertrophy. The experiments were performed on Sprague-Dawley rats, 2K1C hypertension model was prepared by constricting the left renal artery with a sliver clip. Animals were then divided into four groups, 5 mg/kg, 10 mg/kg, 15 mg/kg and the control, pear pectin and apple pectin solutions were supplied with them. The blood pressure was more decreased in pear pectin 10 mg/kg than in apple pectin. The plasma ANP was decreased in pear pectin 10 mg/kg, and renin was increased in same concentration of drug. Cardiac hypertrophy had a tendency to decrease in pear pectin 15 mg/kg, but was not statistically significant compared to control group.

Effect of Calcium-Added Cellulose Coatings on the Firmness of Plums (칼슘을 첨가한 셀롤로우스 코팅이 자두의 경도에 미치는 효과)

  • Song, Tae-Hee;Kim, Chul-Jai
    • Korean Journal of Food Science and Technology
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    • v.31 no.1
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    • pp.91-98
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    • 1999
  • Plums (Prunus salicina L.) were coated with calcium-treated methylcellulose (CaMC) and hydroxypropylmethylcellulose-15 (CaHPMC-15). Respiration rate, weight loss, pH and titratable acidity, texture, and sensory characteristics were compared during 8-day storage period at $30^{\circ}C$. Weight loss and respiration rate of plums coated with CaMC and CaHPMC-15 were lower than those of control plums during storage while pH increased and titratable acidity slowly deceased during storage. Mechanical firmness was significantly deceased during storage, in which CaMC and CaHPMC-15-coated plums were harder than the control. Amount of soluble pectin, and pectin esterase and polygalacturonase activities as the markers of softening commonly increased with storage, but both CaMC and CaHPMC-15-coated plums were not found those increases. Sensory evaluation showed that the flesh softening of plums decreased during storage, and that in the coated plums exhibited the harder flesh. In conclusion, introduction of CaMC and CaHPMC-15 coatings on plums decreased respiration rate and contributed to delay of flesh softening, and the significant difference in postharvest storage quality of the both plums were not found between CaMC and CaHPMC-15 coatings.

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Varietal Variation in Structure and Physical Characteristics of Rice Endosperm Strach (쌀 배유전분구조 및 물리적 특성의 품종 변이)

  • 강미영;최해춘
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.6
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    • pp.513-523
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    • 1993
  • Varietal difference in structural and physical characteristics of rice starch and interrelationships between these starch properties and the physicochemical components of rice grains were investigated for twenty four rice varieties, which were considerably different in amylose content, gelatinization temperature and gel consistency, to obtain the basic informations for diversifying the utility of rice grains and for developing various rice cultivars adaptable to rice food processing. Wave length of maximum absorbance for blue color reaction of starch-I$_2$ complex was 520nm for glutinous rice and 560~600nm for nonglutinous rice. The higher amylose rice showed the longer wave length but there was considerable difference of the wave length among rice materials with similar amylose content. Varietal difference in frequency distribution of debranched starch molecules by isoamylase hydrolysis was largest in amylose fraction, the longest fraction(Fr. I), and was next bigger in intermediate fraction(Int.Fr.) and the ratio between short and long glucose chain of amylopectin fraction(Fr. III /Fr. II). The Fr. III / Fr II ratio was higher in glutinous rice than in nonglutinous one but it was considerably different among nonglutinous rice varieties without any connection with amylose content. The lower alkali digestible rice showed the longer half-hydrolysis time in acid solution. Both soft and hard gel rice varieties in gel consistency test revealed significant varietal difference of 50% acid hydrolysis time. The harder rice in gel consistency exhibited the less frequency of short branch fraction of amylopectin (Fr. III) and the higher amylose content. Twenty four rice varieties were cleary classified by upper two principal components contracted from structural or physical traits of rice starch and some physicochemical properties of rice grain by principal component analysis. The 1st and 2nd principal components can be characterized to components related with amylose and amylopectin structure respectively.

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Changes in Textural Properties of Korean Radish and relevant Chemical, Enzymatic Activities during Salting (염장과정 중 무의 조직감과 이와 관련된 화학적, 효소활성 변화)

  • Rhee, Hee-Seoup;Lee, Gui-Ju
    • Journal of the Korean Society of Food Culture
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    • v.8 no.3
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    • pp.267-274
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    • 1993
  • This study was aimed to investigate the changes in textural properties of Korean radish and relevant chemical, enzymatic activities during salting. During salting, pH was decreased and total acidity was increased. The maximum compression and puncture forces of Korean radish were decreased significantly whereas cutting force was increased. From the force-distance curves, the break point and maximum force point disappeared in salted Korean radish whereas these appeared apparently in fresh one. Also, the number of peak obtained by three types of test from salted Korean radish was decreased. Hot water soluble pectin and 0.4% Na-hexametaphosphate soluble pectin were increased whereas 0.05 N-HCl soluble pectin were decreased significantly. Polygalacturonase activity were increased in Korean radish solid(RS) and Korean radish juice(RJ) until 4 days of salting. Pectin esterase activity were decreased in RS and RJ. Cx-cellulase activity did not appear initially, however, they began to show their activities after 2 days of salting and were increased in RJ although it was low.

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Preparation of Jelly Using Enzyme Soluble Extracts of SeaMustard(Undaria pinnatifida) (미역의 효소추출물을 이용한 젤리의 제조)

  • Park, In-Bae;Kim, Seon-Jae;Ma, Seung-Jin;Park, Jeong-Wook;Jung, Soon-Teck
    • Journal of the Korean Society of Food Culture
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    • v.20 no.4
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    • pp.421-425
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    • 2005
  • The difference on compositions of enzyme extracts which were produced with two kinds of enzyme, celluclast and pectinex, and from sea mustards prepared with two kinds of pretreatments, heating and non-heating treatment, were investigated. The moisture compositions of enzyme extracts from the heating sea mustard were little higher than that of nonheating sea mustard, although the compositions of crude ash and pectin were no significant differences between two kinds of different pretreatment extracts. The supernatant extract ratio was slightly higher level in the heating pretreatment enzyme extracts than non-heating pretreatment extracts and the level of extracts prepared with only celluclast enzyme was higher than using only pectinex and the mixture of two enzymes. The general sensory evaluation value of jelly made from non-heating pretreatment extracts was higher than that from heating pretreatment extracts except on the taste and the texture.

A Study on Mineral and Alginic acid Contents by Different Parts of Sea Mustards(Undaria pinnatifida) (미역의 부위별 무기성분 및 알긴산 함량에 관한 연구)

  • Lee, Yeon-Jung
    • Journal of the Korean Society of Food Culture
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    • v.19 no.6
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    • pp.691-700
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    • 2004
  • This study was conducted to examine the proximate composition, alginic acid and mineral contents and the types of calcium and magnesium in the parts(frond, stipe, sporophyll) of sea mustards. Carbohydrates and the crude fat was the highest in sporophyll. The contents of alginic acid were $25.9{\sim}32.2%$. Total alginic acid(32.2%) and insoluble alginic acid in water(27.7%) was e highest in sporophyll. Calcium and magnesium was the highest in frond. Phosphorous, potassium and sulfur was the highest in sporophyll. Iron, zinc and mangane was the highest in frond. Ca/P ratio in frond and stipe was about $1.7{\sim}1.8:1$ levels. Calcium and magnesium soluble in sodium chloride was the highest in sea mustards. Calcium and magnesium soluble in water was the highest in frond. Calcium and magnesium soluble in hydrochloric acid was the highest in sporophyll.

Physicochemical Properties and Gel-forming Properties of corn & Red bean crude Starches (옥수수와 팥 조전분의 이화학적 특성 및 겔 형성)

  • 노정해;이혜수
    • Korean journal of food and cookery science
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    • v.4 no.1
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    • pp.1-8
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    • 1988
  • The physicochemical properties and gel-forming properties of corn & red bean crude starches were investigated. The results were as follows: 1. The shape of corn crude starch granule was polygonal and the mean value of minor axis and major axis were $11.5\mu\textrm{m}$ and $14.9\mu\textrm{m}$, respectively. In the meantime, the shape of red bean crude starch granule was oval and the mean value of minor axis and major axis were $22.3\mu\textrm{m}$ and $31.4\mu\textrm{m}$. 2. Amylose content of corn and red bean refined starch were 16.52 and 43.61% respectively. 3. Blue value of corn and red bean crude starch were 0.099 and 0.842, respectively. 4. Amylose of corn had molecular weight of 107,000 and degree of polymerization of 660. Amylopectin had degree of branching of 6.9 per 100 glucose units and glucose units of 14.6 persegment of amylopectin. Amylose of red bean had molecular weight of 118,000 and amylopectin had degree of branching of 5.2. 5. Water binding capacities of corn and red bean starch were 238.5 and 284.8. 6. Both swelling powers of corn and red bean starch were increased rapidly from $70^{\circ}C$ to $90^{\circ}C$. 7, Gelatinization of corn and red bean were 75.6 and $61.8^{\circ}C$. 8. Brabender hot-paste viscosities of corn at 6% and 8% showed the similar amylogrm patterns with peak viscosity. And red bean had no peak viscosity. 9. The difference of sensory characteristics for ‘Mook’ and kidney bean & red bean starch gels was significant.

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