• Journal of the Korean Society for Precision Engineering
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• v.12 no.10
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• pp.89-101
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• 1995

선형 탄성 등방성 물체 내에 있는 일반적인 복합모드 크랙 문제들을 해석하기 위한 이중 경계적분방정식의 일반식과 계산해법이 제시되었다. 크랙면이 포함된 물체 해석에 있어서 유일한 해를 얻기 위하여, 한 면상의 점에는 변위 경계적분방정식이 적용되었고 마주하고 있는 상대면 상의 점에는 인력 경계적분방정식이 적용되었다. 인력 및 변위 경계적분방정식의 강특이해 및 초특이해 적분항들은 수치해법을 적용하기 전에 정상화되었다. 정상화과정 중 보정되는 강특이적분항이 상대 크랙면 상의 특이해 요소를 따라 직접 적분되는 것을 격리시키기 위하여, 특이해 적분 경로를 완만한 곡면으로 우회시킨 가상의 비특이해 보조경계로 대치하여 적분값을 계산하였다. 제시된 해법의 정확성과 효율성을 예시하기 위하여, 2차원 및 3차원 크랙 문제의 변형 후 모습과 응력강도계수 계산 결과를 보였다.

• The Journal of the Korean Society for Microbiology
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• v.16 no.1
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• pp.39-48
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• 1981

A direct complement fixation test supplemented with procomplementary porcine serum was studied using anticomplementary human, rabbit and bovine serum against Mycobacterium tuberculosis. Procomplementary activity of porcine serum varied with porcine individual and affected by anticomplementary antiserum. The procomplementary titre of porcine serum against rabbit, human and bovine serum ranged from 1:5 to 1:40. By means of complement fixation test supplemented by porcine serum, the specific complement-fixing antibody to both tuberculopolysaccharide and/or tuberculoprotein antigen was readily differentiated from the anticomplementary antibody titre.

• Research in Plant Disease
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• v.12 no.1
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• pp.25-27
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• 2006

Rice dwarf phytoreovirus (RDV), a member of the family Reoviridae has a genome composed of 12 segmented dsRNAs designated as 51 to 512 with an increasing order of mobility in polyacrylamide gel electrophoresis (PAGE). RDV encode 12 structural and non-structural proteins, $P1{\sim}P12$ which are encoded by the $S1{\sim}S12$ segments of the dsRNA genome, respectively. In this experiment, we confirmed in situ localization of RDV particles and P12 in cytoplasm of infected rice plant. We observed specific reaction of the gold particles using virus particle and P12 protein specific antiserum with protein A-gold immunolabelling in electron microscope. It was observed that gold particles specifically react to virus particles in cytoplasm in case using the antiserum for virus particles. In the case of antiserum for P12 protein, gold particles sporadically existing on cytoplasm without existing in organelle of cytoplasm specifically. As this result, RDV P12 protein encoded by S12 located in cytoplasm.

• Korean Journal of Food Science and Technology
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• v.38 no.1
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• pp.93-98
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• 2006

Effects of anti-Helicobacter pylori IgY powder on H. pylori infection were evaluated 3 and 7 weeks after powder feeding by urease, PCR, and histological tests, and specific IgG assay of murine gastric tissue using mouse model. To produce anti-H. pylori IgY powder, laying hens were immunized with H. pylori prior to egg yolk harvest. C57BL/6 mice showing high response to H. pylori were infected with H. pylori and fed with the anti-H. pylori IgY powder. In urease and PCR tests, urease activity and gene count of anti-H. pylori IgY powder-fed group significantly decreased in comparison with control. Histological results indicated anti-H. pylori IgY powder effectively protected mice from H. pylori.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.10a
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• pp.240-240
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• 2003

V-노치 균열에서 열하중이 작용하는 경우는 비제차형 경계조건의 문제가 되고, 이 조건에 대한 방정식의 일반해를 구하기 위해서 재차형 연립방정식에 대한 일반해(Homogeneous solution)와 비제차형 연립방정식에 대한 특수해(Particular solution)의 두 가지 해를 구할 수 있다. 이들 해는 V-노치 균열에 대한 고유치가 되고 이 고유치가 중복근을 가지게 되는 경우에는 로그항(1n[r])이 나타나게 되고 이 항에 의해서 응력을 무한대로 발산시키므로 이를 대수응력특이성이라 한다. 열하중이 작용할 때 대수응력특이성을 나타내는 로그항의 계수가 영(0)이 되어 대수응력특이성이 사라지게 되므로 V-노치 선단에서의 응력특이성은 고유치와 그에 대한 고유벡터에 의해 결정된다. 본 논문에서는 비정상상태 열하중이 가해지는 등방성 이종재료 내의 V-노치 균열문제에서 패기 각도와 이종재료의 기계적 성질에 의해 결정되는 응력특이성지수를 구하고 이에 대한 응력강도계수를 유한요소해석 프로그램인 ANSYS와 상반일 경로 적분법(RWCIM)을 이용하여 구하였다.

• The Korean Journal of Blood Transfusion
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• v.29 no.3
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• pp.282-290
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• 2018

Background: Anti-E or paired anti-E/-c antibodies can develop in patients with the Rh CDe phenotype. This study examined the differences in transfusion in patients with the CDe phenotype according to formation of anti-E or anti-E/-c antibodies. Methods: Retrospective reviews were carried out on the results of antibody identification tests performed in 2014. The Rh phenotype and antibody specificity were investigated. The transfusion and medical records of patients with the CDe phenotype were examined. Results: In total, 76 patients were included in the review. Of these 76 patients, 38 (50.0%) were of the CDe phenotype. Anti-E antibodies were the most frequent (60.5%), followed by anti-E/-c antibodies (23.7%). The total transfusion units and platelet transfusion units were significantly higher in patients with anti-E/-c antibodies (P=0.028 and P=0.01, respectively). The distribution of categorized diseases was similar in the patients with the anti-E and anti-E/-c antibodies. A frequency of transfusion episodes greater than or equal to four was higher in patients with hepatobiliary diseases (85.7%). Conclusion: In CDe phenotype patients, platelet transfusion was significantly higher in the anti-E/-c positive group than the anti-E positive group, indicating that platelets play a role in red blood cell alloimmunization. Because E is the most immunogenic antigen in Korea, it is important to define the disease group, in which patients with CDe phenotype require a transfusion of E and c-negative blood.

• Parasites, Hosts and Diseases
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• v.32 no.2
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• pp.85-92
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• 1994

A seroepidemiologic observation of anti-Spirometrc erinacei plerocercoid (sparganum) antibody (IgG) in serum was made in normal adult and epileptic patients in Korea from february, 1987 to September, 1990. Sera were tested by enzyme-linked immunosorbent assay (ELISA) for anti-sparganum antibody together with anti-Taenic soEiun metacestode, and anti-Parusonimus westermcni antibodies. Sera reacted positively to sparganum antigen only were considered. Positive rate for anti-sparganum antibody in 850 normal adults was 1.9% (standardized rate by provincial population was 1.7%). In 2,667 randomly selected patients of epilepsy at 28 local centers of the Changmi Club, positive rate was 2.5% (standardized rate: 2.3%). In both normal adult and patient groups, the higher antibody rates were observed in Kangwon and Chonnam Provinces. Positive rates were 10 times higher in male than in female in normal adults and 4.5 times in male epileptic patients. The rates were elevated especially with age over 30-year. odd ratio of the antibody was 1.32 which indicated an ambiguous etiologic factor for epilepsy.

• Parasites, Hosts and Diseases
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• v.30 no.4
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• pp.323-328
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• 1992

Analysis of siR isolates of Trichemenes veginalis was carried out with sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunoelectrotransfer blot (EITB). Trichloroacetic acid-treated antigens of the 6 isolates revealed 25 protein profiles ranging 12~170 kDa of molecular weight in SDS-PAGE. In EITB, the specific immunogenic bands were visualized at 51 kDa and 96 kDa when HY-1 antigen was probed with difFerent mice sera immunized with 6 isolates of T. vaginalis. The banding patterns with different sera showed isolate-to-isolate variability. In EITB, homologous antigen (HY-1) did not show any enhanced response in reacting to homologous antiserum(HY-1) when 6 isolates of T. vaginalis were probed with a single serum (HY-1). It is assumed that the different banding patterns of six isolates show isolate-to-isolate variability and immunogenic common bands in 41, 47, 74 and 9:k kDa on EITB may connote the important significance on immune response in T. vaginalis infection.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.14 no.3
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• pp.381-390
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• 2001

In this paper, a new improved crack analysis technique by Element-Free Galerkin(EFG) method is proposed, in which the singularity and the discontinuity of the crack successfully described by adding enrichment terms containing a singular basis function to the standard EFG approximation and a discontinuity function implemented in constructing the shape function across the crack surface. The standard EFG method requires considerable addition of nodes or modification of the model. In addition, the proposed method significantly decreases the size of system of equation compared to the previous enriched EFG method by using localized enrichment region near the crack tip. Numerical example show the improvement and th effectiveness of the previous method.

• Korean Journal of Clinical Laboratory Science
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• v.54 no.1
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• pp.38-48
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• 2022

Carcinoembryonic antigen (CEA) is an oncofetal antigen primarily detected in the peripheral blood of cancer patients, particularly in those with colorectal cancer. CEA is considered a valuable target for antigen-specific immunotherapy. In this study, we induced the anti-tumor immunity for CEA through the administration of a dendritic cell (DC) vaccine. However, there was a limitation in inducing tumor regression in the DC vaccinated mice. To enhance the efficacy of anti-tumor immunity in MC38/CEA2 tumor-bearing mice, we evaluated the effects of DC vaccine in combination with cyclophosphamide (CYP). Administration of CYP 100 mg/kg in mice resulted in significant inhibition of tumor growth in the 2-day tumor model, whereas a lower inhibition of tumor growth was seen in the 10-day tumor model. Therefore, the 10-day tumor model was selected for testing chemo-immunotherapy. The combined CYP and DC vaccine not only increased tumor antigen-specific immune responses but also induced synergistic anti-tumor immunity. Furthermore, the adverse effects of CYP such as weight loss and immunosuppression by regulatory T cells and myeloid-derived suppressor cells showed a significant reduction in the combined chemo-immunotherapy treatment compared with CYP alone. Our data suggest that chemoimmunotherapy with the DC vaccine may offer a new therapeutic strategy to induce a potent anti-tumor effect and reduce the adverse effects of chemotherapy.