• Korean Journal of Microbiology
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• v.41 no.3
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• pp.168-176
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• 2005

Vibrio vulnificus, one of the marine bacterial pathogens causing septicemia, was detected using molecular methods, namely, PCR and/or Southern hybridization, and real-time PCR. Extracted and purified total DNAs by using commercial kits were used as templates for PCR. Multiplex-PCR was conducted by employing three sets of primers for the genes, hemolysin (vvhA), phosphomannomutase (pmm), and metalloprotease (vvpE), for V vulnificus virulence. The presence of DMSO ($5\%$) and BSA ($0.1\%$) in PCR reaction mixture improved a detection efficiency by higher PCR band intensities. TaqMan real-time PCR was carried out by using gene segment of vvhA as a target. Detection limit of PCR/Southern hybridization without enrichments was to be around $10^2\;cells\;g^{-1}$ of sample. However, those three methods using the enrichment at $35^{\circ}C$ in APW showed high sensitivity ($2\~10\;cells\;g^{-1}$ of sediments). Highly sensitive detection of V vulnificus by real-time PCR was achieved within $5\~6$ hr, whereas the detection by PCR/Southern hybridization required about 36 hr. Thus, it was evident that real-time PCR is the most rapid and efficient method for detecting V vulnificus in tidal flat sediments.

• Journal of the Korean Society for Marine Environment & Energy
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• v.19 no.2
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• pp.137-143
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• 2016

The sedimentary environment and sediment were surveyed at the West-Northern site of Chagwi-do nearby Jeju Island for the design of the embedded suction anchor system of 10 MW-class floating wave-offshore wind hybrid power generation system. According to the classification scheme of Chough et al.[2002], the echo type of the seismic profiles using the chirp III was classified. As a results, the center and west-northern area of survey site were proved to be type I-3 where subbottom layer with thickness 5~15 m exists under the flat seafloor. On the other hands, the east-southern area were regarded to be type I-1, I-2 and III-1 where seafloor reflection is much stronger than type I-3. Also, the physical tests (unit weight, moisture content, grain size, liquid limit, specific gravity) were performed with samples taken from 8 fixed locations. It is found that the sand (SP), the sand blended with silt (SM) and the mixture of SP-SM are distributed uniformly on the survey area.

• Proceedings of the Korean Society of Disaster Information Conference
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• 2015.11a
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• pp.162-165
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• 2015

본 연구는 ${\bigcirc}{\bigcirc}$ 공구 공유수면 매립공사 수행 중에 매립토량의 부족이 발생함에 따라 이를 대상으로 원인을 분석하고자 수행하였다. 매립지의 토량부족 원인을 파악하기 위해 설계 및 계측자료를 수집하여 시공 현황을 파악하고 지반조사를 통해 해안지역과 매립지반의 시료를 채취하여 성상분석과 원지반에 대한 공학적 특성을 비교 검토하였다. 특히. 현장 시추조사에 의한 경계면 분석과 토질 종류를 점성토, 사질토, 부유토로 구분하고 침하량 공식에 따라 발생량을 산출하였다. 구역의 구분은 설계 시와 동일한 조건에서 침하량을 계산하였고 설계 시 지반고가 침식과 퇴적으로 인해 일부 변경되어 설계시 지반고와 착공시 지반고를 구분하여 기재하고 각각의 침하량을 비교하였다.

• Economic and Environmental Geology
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• v.37 no.1
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• pp.121-131
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• 2004

Removal efficiencies of soil washing and soil improvement processes to remediate farmland soils and stream deposits around Goro abandoned mine were investigated with batch and column experiments. For As-contaminated farm-land soils around Goro mine, batch tests to quantify As extraction rate from contaminated soils and lime treated contaminated soils were performed. The contaminated soil mixed with lime decreased As extraction rate less than one fourth, suggesting that the soil improvement method mixed with lime dramatically decrease As extraction rate. A storage dam will be constructed in the lower part of the main stream connected to Goro abandoned mine and the amount of As extracted from the bottom soils of reservoir could be the main source to contaminate water of reservoir. The decrease of As extraction amount from the bottom in reservoir, caused by the application of the soil improvement method was investigated from the physically simulated column experiment and results showed that As extraction rate decreased to one forty when 1％ lime mixed soil improvement was applied to contaminated soils. For contaminated stream deposits connected Goro mine, the removal efficiency of the soil washing method was investigated with batch experiments. Hydrochloric acid, citric acid, acetic acid and distilled water were used as soil washing solution and 0.01, 0.05, 0.1, 0.5, 1.0 N of washing solution were applied to extract As. When washing with 0.05 N of hydrochloric acid or citric acid, more than 99.9% of As was removed from stream deposits, suggesting that As contaminated stream deposits around Goro mine be successfully remediated with the soil washing process. Total volumes of contaminated soils and deposits needed for remediation were calculated based on three different reme-diation target concentrations and the operation cost of soil washing for calculated soil volumes was estimated. Results from this research could be directly used to make a comprehensive countermeasure to remediate contaminated area around Goro mine and also many contaminated areas similar to this research area.

• Journal of the Korean Society of Marine Environment & Safety
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• v.22 no.2
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• pp.246-252
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• 2016

In this study, conceptual design of the fore-body hull form of catamaran type dredging vessel was performed that can effectively remove the contaminated sediments in coastal seabed. The hull form was simpled for the easy hull construction and the resistance performance was investigated to find out the effect of hull form parameters between variation of waterline and angle of entrance, etc. The relation between resistance performance and characteristics of free surface flows according to variation of bow forms was investigated by model testing in the circulating water channel and using Ansys CFX. The improvement of ship resistance performance to the wave resistance decrease due to improved wave pattern has been verified according to move the stem and the volume of the shoulder to the fore part of the vessel.

• Proceedings of the Korea Water Resources Association Conference
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• 2006.05a
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• pp.1323-1327
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• 2006

일반적으로 화력발전소는 원활한 원료 및 냉각수의 공급과 환경적인 요인으로 인해 내륙보다는 해안에 위치하고 있다. 발전소의 안정성을 확보하기 위해서는 고열의 발전기를 냉각하는 냉각설비들이 반드시 필요하며 이를 위해 취수로를 통한 냉각수의 원활한 공급이 이루어져야 한다. 이러한 냉각수 공급은 대용량 펌프에 의해 이루어지며, 취수로 구조물의 형상 및 냉각수의 흐름조건에 따라 성능과 수명이 좌우된다. 특히 해안에 위치하는 지리적 여건상 직접취수가 많으며 이러한 경우 유사가 취수로에 유입되어 펌프 흡입 시 균열과 임펠러 마모 등을 유발시켜 펌프의 안전에 악영향을 미치게 된다. 따라서 취수로에 유사의 퇴적을 유도하는 침사지를 설계하게 되며 Hydraulic Institute Standards(1998)에서는 허용설계유속을 0.2 m/s 이하로 정하고 있다. B 발전소는 직경 3 m의 대형 취수관로 3개를 통해 매우 빠른 유속으로 취수로에 유입되는 냉각수 계통 구조물을 가지고 있어 침사지 내 유속이 허용기준을 초과하였다. 본 연구에서는 침사지내 유속을 만족시키기 위해 다양한 에너지 소산 구조물 설계방안을 제시하였다. 각 설계방안에 따른 흐름 특성 및 유속 분포를 파악하고 common bay 내에서의 난류특성을 분석하여 비교.검토하였다. 수리모형실험 결과를 통해 각각의 설계방안에 따른 침사지의 효율을 평가하였으며 최적의 에너지 소산 구조물에 대한 설계방안을 제안하였다.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.31 no.5B
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• pp.467-474
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• 2011

Debris flow is one of the most hazardous natural processes in mountainous regions. The degradation of discharge capacity of drainage facilities due to debris flows may result in damages of properties and casualty as well as road. Understanding and accurate reproducing flow behaviour of debris flows at various conditions, such as sediment volume concentration and approaching channel and culvert slopes, are prerequisite to develop advanced design criteria for drainage facilities to prevent such damages. We carried out a series of laboratory experiments of debris flows in a rectangular channel of constant width with an abrupt change of bottom slope. The experimental flume consists of an approaching channel part with the bed slope ranging $15^{\circ}$ to $30^{\circ}$ and the test channel with slope ranging from $0^{\circ}$ to $12^{\circ}$ which mimics a typical drainage culvert. The experiments have been conducted for 22 test cases with various flow conditions of channel slopes and sediment volume concentration of debris flows to investigate those effects on the behaviour of debris flows. The results show that, according to sediment volume concentration, the depth of debris flow is approximately 50% to 150% larger than that of fresh water flow at the same flow rate. Experimental results quantitatively present that flow behaviour and deposit history of debris flows in the culvert depend on the slopes of the approaching and drainage channels and sediment volume concentration. Based on the experimental results, furthermore, a logistic model is developed to find the optimized culvert slope which prevents the debris flow from depositing in the culvert.

• Proceedings of the Korean Geotechical Society Conference
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• 1999.11c
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• pp.169-178
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• 1999

본 논문에서는 실내에서 실시한 Filtration실험을 통하여 지하수 흐름하에서 이탈한 풍화 잔류토의 세립자가 흙-토목섬유 복합체를 통과하면서 발생하는 이동 (Transportation), 퇴적 (Deposition), 그리고 폐색 (Clogging)되는 과정을 분석하였다. 본 연구에서는 한국의 풍화 잔류토에 적합한 토목섬유 배수재의 새로운 설계기준을 제시함으로써 풍화 잔류토상에 축조되는 터널 구조물 시공시 광범위하게 사용되고 있는 토목섬유재인 부직포의 투수 및 배수특성과 세립토사에 의한 폐색 (Clogging)영향을 분석할 수 있게 되었다.

• Proceedings of the Korean Society of Coastal and Ocean Engineers Conference
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• 1996.10a
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• pp.133-136
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• 1996

최근 자원의 효율적인 이용을 위한 연안 개발이 활발하게 진행되고 있는데, 이러한 연안 개발사업은 초기의 막대한 투자비와 유지 관리가 소요되기 때문 파랑, 흐름, 퇴적물 이동, 조석 등의 설계 환경 요인을 정확하게 예측하는 것이 필요하다. 해수 유동에 관한 수치모형 실험이라 함은 조석을 비롯한 해수의 움직임을 1차원, 2차원, 3차원적으로 재현하는 것을 말한다. 본 연구에서는 영종도 연육교 건설에 따른 서해안 일대의 조석 체계의 변화에 대해 현장 관측 및 2차원적 수치모형 실험을 통해 정확한 모양을 재현하고자 한다. (중략)

• Proceedings of the Korean Society of Coastal and Ocean Engineers Conference
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• 2002.08a
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• pp.31-34
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• 2002

Bragg 반사는 입사된 주기파의 진폭이 해저지형의 진폭의 2배가 될 때 파랑의 반사율이 공명현상(resonance)에 의해 매우 커지는 물리적 현상을 말한다. Bragg 반사를 응용할 경우, 외해로부터 입사되는 파랑 에너지의 상당량을 반사시킬 수 있으므로 항만이나 방파제 등의 해안구조물을 경제적으로 설계하고 보호할 수 있다. 또한, 해안선 보호 및 불필요한 침식이나 퇴적 등을 고려한 연안개발계획의 효율적 수립이 가능하다. (중략)