• Food Science and Preservation
• /
• v.20 no.5
• /
• pp.744-750
• /
• 2013

The indigenous Saccharomyces cerevisiae strains S13 and D8 were isolated at the microbial succession stage during spontaneous fermentation of Campbell Early wine as a resistant to potassium metabisulfite and a high sugar concentration. In this study, the fermentation characteristics of Campbell Early wine were investigated and compared with those of S. cerevisiae W-3, an industrial wine yeast. Alcohol production by the two strains was delayed at the initial fermentation stage, but increased fast when the fermentation continued. After the fermentation, the S13 and D8 wines contained 12.6% and 13.2% (v/v) alcohol, respectively, which were significantly higher than the alcohol content of the W-3 wine (12%, v/v). No marked differences were observed in the residual soluble solid content and the pH. However, the S13 and D8 wines showed high levels of total acid content, including malic and lactic acids. Especially, the lactic acid content was 8.9-fold in the S13 wine and six-fold in the D8 wine, compared with that of the W-3 wine. The two strains produced a higher level of acetaldehyde and a lower amount of methanol in the wine than the W-3 strain. The iso-Butanol content was lower in the two indigenous yeast wines with similar levels of n-propanol and iso-amyl alcohol contents than that in the W-3 wine. In the sensory evaluation, the S13 and D8 wines had higher scores for their color, flavor, taste and overall preferences than the W-3 wine. Especially, the S13 and D8 wines had much higher scores than the W-3 wine for flavor and color, respectively.

• Korean Journal of Soil Science and Fertilizer
• /
• v.33 no.3
• /
• pp.193-204
• /
• 2000

In order to get some basic data on environmental friendly function by Korean organic farming, the chemical characteristics of soil were determined on 100 farm cultivating site in Paldang watershed area of Great Seoul. The EC and content of $NO_3-N$ and Av. $P_2O_5$ in topsoil(0~30cm) showed $2.30dS\;m^{-1}$, $82mg\;kg^{-1}$, $918mg\;kg^{-1}$ in the soil cultivated chinese cabbage. $2.29dS\;m^{-1}$, $86mg\;kg^{-1}$, $954mg\;kg^{-1}$ in the soil of lettuce, $1.83dS\;m^{-1}$, $66mg\;kg^{-1}$, $1114mg\;kg^{-1}$ in the soil of crown daisy. These salt accumulation(EC) and the high concentration of mineral content in topsoil such as nitrate and phosphate showed the soils of organic farming were contaminated by practice of organic farming for the maintenance strategy of soil fertility. The $NO_3-N$ and Av. $P_2O_5$ in the subsoil(30~60cm) showed $75mg\;kg^{-1}$ and $641mg\;kg^{-1}$, $72mg\;kg^{-1}$ and $466mg\;kg^{-1}$, $42mg\;kg^{-1}$ and $873mg\;kg^{-1}$ in soil cultivated chinese cabbage, lettuce and crown daisy respectively. It indicates eventually the high concentration of nitrate and phosphate in topsoil caused penetration to subsoil, and the high concentration of mineral contents in subsoil indicate the potential risk of leaching of ground water by Korean organic farming. The positive correlation at 1% between EC and $NO_3-N$, $K_2O$, T-C, $P_2O_5$ and T-N show the salt accumulation in the both soil depth of Korean organic farming were caused by minerals such as $NO_3-N$, $K_2O$, T-C, $P_2O_5$ and T-N by overuse of organic fertilizer.

• Proceedings of the Korean Information Science Society Conference
• /
• 2002.04a
• /
• pp.82-84
• /
• 2002

신뢰성이 높은 분산 시스템은 고장 발생 시 고장을 탐지하고, 다른 관련된 노드들에게 고장을 알려주어서 적절한 처리를 할 수 있어야 한다. 기존의 act 와 time-out 을 사용한 고장 탐지방법은 수신되지 않는 ack 에 대한 부하가 높은 단점을 가지고 있다. 높은 신뢰성을 요구하는 분산 실시간 시스템에서는 데드라인을 준수하기 위해 고장처리에 대한 time-bound 를 결정할 수 있어야 하므로, 기존의 ack 와 time-out 에 기반한 고장방법을 사용하기에 부적절하다. 따라서 본 논문에서는 신뢰성 있는 분산 실시간 멀티캐스트 프로토착에 결합된 고장 탐지 기법으로서 사용될 기존의 고장 탐지 방법을 대상으로 고장 탐지 방법을 실험하고, 그 결과를 분석하여 고장탐지 및 고장처리 지연시간이 데드라인을 보장할 수 있는지 검사한다.

• Korean Journal of Soil Science and Fertilizer
• /
• v.21 no.2
• /
• pp.141-148
• /
• 1988

Though the cultivation history of soybean in Korea is relatively long, taxonomical study on symbiont nodule bacteria, Rhizobium japonicum is not carried out yet systematically. This work was for the taxonomical study on Korean native R. japonicum by recognizing isolates seroimmunologically as well as for the elucidation of its affinity with host soybean variety. Twenty seven isolates from 13 soybean cultivars cultivated at Seoul National University's experiment field and 6 strains of R. japonicum preserved in our laboratory have been tested. Tube agglutination test, agglutinin adsorption test, and gel immune diffusion test were used. The results obtained are as follows: 1. Twenty five isolates and strains of R. japonicum among 33 were classified into 4 serogroups and identified as indivisual serotype. 2. Two isolates isolated from Hill and Milyang cultivars, 2 isolates from Bangsa and Jangbaek, and 4 isolates from Paldal, Sae-al, and Jangbaek were identified as the same serotype respectively. 3. Seroimmunological tests may be adapted for the elucidation of the affinities between the strains and soybean cultivars as well as strain recognition and systematic classification of Korean native R. japonicum.

• Food Science and Preservation
• /
• v.21 no.6
• /
• pp.851-858
• /
• 2014

Muscat Bailey A (MBA) wine was fermented using the indigenous Korean Saccahromyces cerevisiae strains S13 and D8, and the fermentation characteristics were compared with those of S. cerevisiae W-3, an industrial wine yeast. The strains S13 and D8 showed delayed alcohol fermentation compared with the W-3 strain, but the final alcohol contents of the S13 and D8 wines after fermentation were similar to those of the W-3 wine. The S13 wine showed significantly lower malic-acid content than the W-3 wine, but the D8 wine showed a similar level. Both the wines fermented using the S13 and D8 strains showed significantly lower acetaldehyde, methanol, and fusel oil contents, including n-propanol, iso-butyl alcohol, and iso-amyl alcohol, compared to the W-3 wine. Especially, the methanol content was 98.6 mg/L in the S13 wine and 112.0 mg/L in the D8 wine, which were much lower than 192.8 mg/L in the W-3 wine. The S13 wine obtained the highest score in terms of color among the three wines in the sensory evaluation, with lower Hunter's L, a, and b values compared to the W-3 wine.

• Korean Journal of Environmental Biology
• /
• v.18 no.2
• /
• pp.205-213
• /
• 2000

Laboratory studies have revealed that naturally transformable bacteria develop competence under in situ conditions. Thus, the occurrence of competent bacteria in the environment can be considered as a certainty The persistence of free DNA in natural habitats is influenced by nucleolytic degradation and protection from degradation by adsorption to minerals. Although DNA seeded into natural environment was hydrolysed at substantial rates, but was still detectable at low levels after even several weeks. Compared to the number of laboratory based studies, only a few data have been published dealing with transformation of bacteria in the field. Recently, the potential transfer of recombinant DNA (rDNA) from deliberately or accidentally released bacteria to indigenous microbes has raised biosafety issues, since the persistence of rDNA becomes independent of the survival of its original host and leads to unpredictable, long-term ecological effects. The aim of the present review is to summarise recent literature on horizontal gene transfer (HGT) by transformation among bacteria in both soil and aquatic habitat and special emphasis is placed on recent reports which have addressed HGT among bacteria in the field. [Transformation, Horizontal gene transfer (HGT), recombinant DNA (rDNA), Genetically modified microorganisms (GMMs), Biosafety]

• Economic and Environmental Geology
• /
• v.47 no.1
• /
• pp.61-69
• /
• 2014

As we are trying to in-situ treat (purify or immobilize) heavy metals or radionuclides in groundwater, one of the geochemical factors to be necessarily considered is the value of oxidation/reduction potential (ORP) of the groundwater. A biogeochemical impact on the characteristic ORP change of groundwater taken from the KAERI underground was observed as a function of time by adding electron-donor (lactate), electron-acceptor (sulfate), and indigenous bacteria in a laboratory condition. There was a slight increase of Eh (slow oxidation) of the pure groundwater with time under a $N_2$-filled glove-box. However, most of groundwaters that contained lactate, sulfate or bacteria showed Eh decrease (reduction) characteristics. In particular, when 'Baculatum', a local indigenous sulfate-reducing bacterium, was injected into the KAERI groundwater, it turned to become a highly-reduced one having a decreased Eh to around -500 mV. Although the sulfate-reducing bacterium thus has much greater ability to reduce groundwater than other metal-reducing bacteria, it surely necessitated some dissolved ferrous-sulfate and finally generated sulfide minerals (e.g., mackinawite), which made a prediction for subsequent reactions difficult. As a result, the ORP of groundwater was largely affected even by a slight injection of nutrient without bacteria, indicating that oxidation state, solubility and sorption characteristics of dissolved contaminants, which are affected by the ORP, could be changed and controlled through in-situ biostimulation method.

• Journal of the Korean Institute of Traditional Landscape Architecture
• /
• v.32 no.1
• /
• pp.22-30
• /
• 2014

"ImwonGyeongjeji" is recognized the value which is unparalleled encyclopedia in the world, but that is only evaluate about variously handled with subject area and massive content and also most scholarly research was focusing agriculture which is the domestic situation of published period and "ImwonGyeongjeji" judged as a simple agriculture book. Therefore, this research focus to theory and technique of traditional landscape architecture from science of landscape architecture perspective and it is from "ImwonGyeongjeji" which is known as encyclopedia written by Seo Yu-gu in 19th century Joseon period. For such a contents, "ImwonGyeongjeji" can find a importance that it have value and status for represent of Korea 'landscape architecture statement' which compared with other East Asia in those aspects; 'professional about landscape architecture', 'superiority of composition system', indigenousness suitable for Korea' and 'enhance to superiority value of environmental factor for writing'.

• Journal of the Korean Wood Science and Technology
• /
• v.30 no.2
• /
• pp.134-143
• /
• 2002

According to the result of the experiment, except that Oebal Hanji making, which does not consider the fiber orientation, has a smaller difference between its strength of width and length direction than of Ssangbal Hanii making, there is not a significant difference between Oebal Hanji making and Ssangbal Hanji making. In addition, even though Oebal Hanji making produced indigenous technique is generally felt sturdy because its absolute strength is enhanced by applying fine materials and overlaying several sheets of paper, its relative strength, which is expressed in an index, is not far beyond that of Ssangbal Hanji making Study on the effect of Dochim revealed that Dochim improves remarkably folding endurance and enhances breaking length and the burst strength. Especially, Dochim increase tensile strength. It was also revealed that, indigenous Oebal Hanji making, the higher the number of sheets of paper overlaid is, the higher the tear strength is the increase relatively higher than those of other types of strength. Consequently, Dochim treatment greatly reinforces the imperfect strength of indigenous Hanji. We consider that if we apply Dochim treatment to Ssangbal Hanji making it may bring about abnormal imbalance of strength. We can benefit from the characteristic strength of Oebal Hanji making only when performing paper making and Dochim considering fiber orientation.

• Korean Journal of Culture and Social Issue
• /
• v.12 no.2
• /
• pp.77-100
• /
• 2006

This study investigates happy life experiences and their emotional responses to these experiences with a sample of Korean students and adults. A total of 489 Korean university students and adults completed an open-ended questionnaire developed by the present researchers. Three sets of results have been found. First, while experiences of achievement and failure affect the strength of feelings of happiness or unhappiness, social relationships with family members and friends affect the frequency of feelings of happiness or unhappiness. Second, while strong positive affects are aroused by sense of achievement and pride, frequent positive affects are aroused by feeling of calmness and positive relational emotions (such as Jung - deep affection and attachment). Third, while strong negative affects are aroused by sense of frustration and sadness, frequent negative affects are aroused by anxiety and negative relational emotions (such as the sense of indebtedness and shame). These results indicate that the interpersonal relationships with family members and friends and personal achievements and failures influence the subjective well-being of feeling happy or unhappy with people's lives.