• Title/Summary/Keyword: 탐색적연구

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Phylogenetic Analysis of 680 Prokaryotes by Gene Content (유전자 보유 계통수를 이용한 원핵생물 680종의 분석)

  • Lee, Dong-Geun;Lee, Sang-Hyeon
    • Journal of Life Science
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    • v.26 no.6
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    • pp.711-720
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    • 2016
  • To determine the degree of common genes and the phylogenetic relationships among genome-sequenced 680 prokaryotes, the similarities among 4,631 clusters of orthologous groups of protein (COGs)’ presence/ absence and gene content trees were analyzed. The number of COGs was in the range of 103–2,199 (mean 1377.1) among 680 prokaryotes. Candidatus Nasuia deltocephalinicola str. NAS-ALF, an obligate symbiont with insects, showed the minimum COG, while Pseudomonas aeruginosa PAO1, an opportunistic pathogen, represented the maximum COG. The similarities between two prokaryotes were 49.30–99.78 % (mean 72.65%). Methanocaldococcus jannaschii DSM 2661 (hyperthermophilic and autotrophic, Euryarchaeota phylum) and Mesorhizobium loti MAFF303099 (mesophilic and symbiotic, alpha-Proteobacteria class) had the minimum amount of similarities. As gene content may represent the potential for an organism to adapt to each habitat, this may represent the history of prokaryotic evolution or the range of prokaryotic habitats at present on earth. COG content trees represented the following. First, two members of Chloroflexi phylum (Dehalogenimonas lykanthroporepellens BL-DC-9 and Dehalococcoides mccartyi 195) showed a greater relationship with Archaea than other Eubacteria. Second, members of the same phylum or class in the 16S rRNA gene were separated in the COG content tree. Finally, delta- and epsilon-Proteobacteria were in different lineages with other Proteobacteria classes in neighbor-joining (NJ) and maximum likelihood (ML) trees. The results of this study would be valuable to identifying the origins of organisms, functional relationships, and useful genes.

An Explorative Study on Using the Method of Natural Family Planning (자연적 가족계획방법 사용에 대한 탐색적 연구)

  • Choi, Hee-Chung;Park, Shin-Ae
    • Research in Community and Public Health Nursing
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    • v.12 no.1
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    • pp.226-238
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    • 2001
  • This study was attempted to understand experiences of the users of natural family planning(NFP) by applying Q-methodology. 37 statements were used to Q population through literature research and interviews. For the P sample. I interviewed personally 30 people who had taken a lesson in NFP for over 6 months and had been using and experiencing it autonomously for more than 2 years so far, and asked them to Q-classify the statement cards in order of the degree of approval, from the one they most approved to the one they least approved. Data were coded and input into a computer and were analyzed using QUANL PC Program. In this study, I discovered 4 types of experiences by the users of NFP and named them each according to their features: the first, a type of mutual control with morality, the second, a type of the pursuit of mutual -cooperation, the third. a type of the pursuit of conviction. and the fourth, a type of the perception of health management. The first type said that NFP was a good contraceptive way in which no drug or devices are used, that the husband's cooperation was needed, and that they became able to control sexual desire with morality. The second type perceived that continuous efforts were required to put NFP in practice, that the, husband's cooperation was essential, and that dialogues between husband and wife were also necessary to use it, and as such they regarded mutual cooperation between husband and wife as highly important. On the other hand. the third type perceived that one's conviction played a big part, that knowledge of physiological changes was useful and the ability to sense physical changes improved. and that one's willpower was important. and thus they regarded one's own willpower as most important. The fourth type reported that this method was a good way to control pregnancy and should be taught about at the beginning of sex education. and thus they were emphasizing the importance of NFP as a helpful way to manage one's health. 1. therefore, intend to provide the following suggestions based on the result of this study. 1) It is necessary to develop a program that allows NFP to be applied to sex education program. 2) It is necessary to analyze factors that affect the execution of NFP. 3) It is necessary to analyze causes of failure of those who fail to execute NFP.

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Purification and Characterization of Phospholipase D from Actinomycetes KF923. (방선균 KE923이 생산하는 Phospholipase D의 정제 및 특성)

  • 곽보연;윤석후;김창진;손동화
    • Microbiology and Biotechnology Letters
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    • v.31 no.4
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    • pp.389-394
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    • 2003
  • In order to screen microorganisms producing phopholipase D (PLD) had high transphosphatidylation activity, about 1,000 Actinomycetes strains were isolated from the 63 soil samples, collected over 6 local area in Korea. When the hydrolytic activity in the supernatant was determined, 131 strains produced PLD more than 0.3 U/ml. Among 131 culture broths tested, 23 ones had transphosphatidylation activity higher than 20% and finally one strain (Actinomycetes KF 923), which had highest hydrolytic and transphophadylation activity, was selected. Actinomycetes KF923 showed the highest hydrolytic activity (13 U/ml) and phosphatidylation activity (95%) after 48 h fermentation using the P medium (yeast extract 1%, peptone 1%, glucose 1.5%, glycerol 1%, $CaCo_3$ 0.4%, pH 7.2). PLD was purified from the culture broth of Actinomycetes KF923 and the specific activity of purified PLD was 567 U/mg. The molecular weight of PLD was about 55 kD and the optimum pH and temperature were 6.0 and $60^{\circ}C$, respectively. The stability of PLD toward pH and temperature were high around pH 8.0 and below $40^{\circ}C$. Special metal ions were not necessary to the PLD activity.

Optimization of Post-Processing for Subsequence Matching in Time-Series Databases (시계열 데이터베이스에서 서브시퀀스 매칭을 위한 후처리 과정의 최적화)

  • Kim, Sang-Uk
    • The KIPS Transactions:PartD
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    • v.9D no.4
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    • pp.555-560
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    • 2002
  • Subsequence matching, which consists of index searching and post-processing steps, is an operation that finds those subsequences whose changing patterns are similar to that of a given query sequence from a time-series database. This paper discusses optimization of post-processing for subsequence matching. The common problem occurred in post-processing of previous methods is to compare the candidate subsequence with the query sequence for discarding false alarms whenever each candidate subsequence appears during index searching. This makes a sequence containing candidate subsequences to be accessed multiple times from disk, and also have a candidate subsequence to be compared with the query sequence multiple times. These redundancies cause the performance of subsequence matching to degrade seriously. In this paper, we propose a new optimal method for resolving the problem. The proposed method stores ail the candidate subsequences returned by index searching into a binary search tree, and performs post-processing in a batch fashion after finishing the index searching. By this method, we are able to completely eliminate the redundancies mentioned above. For verifying the performance improvement effect of the proposed method, we perform extensive experiments using a real-life stock data set. The results reveal that the proposed method achieves 55 times to 156 times speedup over the previous methods.

An Image Retrieval Method based on Quantitative Emotion Evaluation on Color Harmony (색채조화의 정량적 감성평가에 기초한 이미지 검색법)

  • Kim, Don-Han;Jeong, Jae-Wook
    • Science of Emotion and Sensibility
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    • v.15 no.1
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    • pp.87-96
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    • 2012
  • This paper proposes a Image retrieval system that searches the closest images to the user's emotional need and displays images with higher ratings of color harmony from Moon-Spencer's Color Harmony Theory first. Once an emotional adjective is placed, the system searches for images with colors that contain more elements derived from Aesthetic Measure results and displays in such order. In order to test reliability of the proposed emotion retrieval method based on Moon-Spencer's Color Harmony Theory, this study compared the order of Aesthetic Measure results with the user satisfaction ratings using 200 sample images. The analysis demonstrated that the participants' average satisfaction on 15 emotion adjectives selected for the study was 5.0 on a 7-point Likert scale. Correlation analyses were performed to test the consistency the orders between Aesthetic Measure values and user satisfaction ratings. Positive correlations above R=.5 were observed in all 14 emotion words except "Clear". These findings prove the potential of the proposed emotion retrieval system based on Moon-Spencer's Color Harmony Theory to effectively reflect user emotion in such visual stimulus search as image database.

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Investigation on Influencing Environmental Factors on Health Status of Korean Septuagenarians Dwelling in Longevity Region in Jeonla Province (전라도 농촌장수지역 거주 70대 노인의 건강상태에 영향을 미치는 환경적 요인에 대한 탐색 연구)

  • Kwak, Chung Shil;Yon, Miyong;Lee, Mee Sook;Oh, Se In;Park, Sang Chul
    • Korean Journal of Community Nutrition
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    • v.19 no.2
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    • pp.142-162
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    • 2014
  • Objectives: To evaluate the critical environmental factors on healthy-aging of Korean people, we investigated the significant factors influencing health status of septuagenarians living in rural area of Jeonla province, known to be one of the representative longevity regions in Korea. Methods: We divided subjects into healthy group (36M/25F) or poor-health group (26M/73F) based on self-reported health status, body mass index, a number of prescription, and blood test data. General characteristics, physical measurements, lifestyle, dietary behavior and nutrient intake, physical health and mental health data were statistically compared between the two groups. Results: Average age was not different between healthy group and poor-health group in men and women, respectively. In men, significantly favorable factors to health were observed to be higher education, regular exercise, higher grip strength and walking function, body mass index (${\geq}18.5kg/m^2$), moderate frequency of drinking and eating-out, non-smoking, normal red blood cell (RBC) count, higher serum dehydroepiandrosterone-sulfate (DHEAS) level, good digestive function and appetite, normal hearing function, regular meals, adequate vegetable and fruit intake, diverse food intake, adequate energy and nutrients (protein, vitamin $B_1$, $B_6$, C and E, folate, niacin, P, Zn and K) intake, higher mini-nutrient status assessment (MNA) score and low level of depression. On the other hand, in women, those were literacy, living arrangement, moderate frequency of drinking, healthy teeth, higher grip strength and walking function, bone mineral density, normal RBC and white blood cell (WBC) count, higher DHEAS concentration, higher MNA score, normal cognition and memory function, having snack and adequate fruit intake. Conclusions: These results could be useful to plan effective strategies to increase health-life expectancy of Korean old people living in rural areas.

Biochemical Changes in Brassica Seedlings Due to Cold Acclimation Treatment (Brassica속 작물 유묘에서 장기 저온 순화처리에 따른 생화학적 변화)

  • Park, Woo-Churl;Oh, Yun-Jin;Nam, Min-Hee
    • Applied Biological Chemistry
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    • v.38 no.3
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    • pp.212-217
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    • 1995
  • This study was aimed for determining the biochemical mechanism of cold tolerance in crops and for searching the biochemical genetic marker related with cold tolerance by the analysis of isozyme pattern. We investigated various biochemical changes induced by the long-term cold acclimation in cold sensitive rape (B. napus) and in cold tolerant 'Sandongchae'(B. campestris) seedlings. The cold shock after long-term cold acclimation to B. napus and B. campestris greatly increased the activities of peroxidase 157% and 50% in root fraction and, 201% and 205% in hypocotyl, respectively. Simultaneously, the activity of superoxide dismutase was largely increased in hypocotyl fraction, too. Protein contents of hypocotyl fractions in B. napus and B. campestris were also increased by 11.4% and 57.8%, respectively. The band of pl 6.4 among peroxidase isozymes newly biosynthesized during long-term cold acclimation was emerged in the hypocotyl fraction of cold tolerant B. campestris as well as in the root of both species. From above and previous results, we presented a model of interconversions of molecular oxygen species due to the cold injury and biochemically inferred the mechanism of cold tolerance in crops.

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An Efficient USR system design and implementation based on the USN (USN을 이용한 효율적인 USR 시스템 설계 및 구현)

  • Jin, Woo-Jeong;Xiao, Huang;Jeong, Dae-Ryeong;Shin, Geuk-Jae;Jung, Hoe-Kyung
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2010.05a
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    • pp.451-453
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    • 2010
  • The world is rapidly evolving in the future society is based on intelligence. In such circumstances, USN(Ubiquitous Sensor Network) to implement the future ubiquitous(Ubiquitous) society have been highlighted as a key infrastructure. For realization of Ubiquitous computing(Ubiquitous Computing), The data recognized from each sensors must be collected on real-time and transferred to applied service so that they may be used as data for providing service to users. USN sensor data on the use for using a numerous sensor data provision service, service provider can publish its service in standardized registry for users to search and use the service registry. However, in previous studies using Web services standards WS-Eventing, and UDDI(Universal Description, Discovery, and Integration) as USR(USN Service Registry) for USN Application Service is unnecessary or insufficient. In this paper, data from sensors that provide information to service providers register their services and use the information for service users to explore and views on the registry of the system was designed and implemented.

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Characteristics of Submarine's Turbulent Wake using Analytical Solution of B(ξ) for the given λ (임의 λ에 대한 근사해 B(ξ)를 이용한 잠수함 난류항적 특성 연구)

  • Lee, Yong-Chol
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.21 no.8
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    • pp.1604-1610
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    • 2017
  • Using analytical solution of $B({\xi})$, I could find out the characteristics of Submarine's turbulent wake for the given various ${\lambda}$, which were unknown facts before. As the results, $B({\xi}){\approx}{\frac{B({\xi})_{max}}{2}}$ and ${{\int}_{0}^{{\approx}0.6}}B({\xi})d{\xi}{\approx}0.85{{\int}_{0}^{1}}B({\xi})d{\xi}$ in the vicinity ${\xi}{\approx}0.6$, there was some dependencies on the given ${\lambda}$ though. The values of ${\lambda}$, in the range of $4{\leq}{\lambda}{\leq}8$, are more suitable to describe submarine's turbulent wake realistically, due to the bases on the quasi equilibrium state of turbulent wake. ${\lambda}$ mainly affects on the radius and detection range of the submarine's turbulent wake on the surface, however, the speed of submarine mainly affect on the duration of the wake rather than shape. If $7{\leq}{\lambda}{\leq}8$, it can be expected that the turbulent wake can be seen on the surface in the West sea, however, snorkeling(or snorkeled) submarine's wake can be found easily in the East sea.

The effect of university students' approach to health information on improvement of health behavior (대학생들의 건강정보 접근이 건강행태 개선에 미치는 영향)

  • Kim, Seung-Dae;Kim, Myung-gwan
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.5
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    • pp.3268-3275
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    • 2015
  • The goals of this study are understanding the state of improvement of university students' health behavior who are about to graduate and have to consider their health for getting a job according to the way of access and achieving health information and suggesting effective way to improve health behavior. 224 people who responded to the survey which was conducted at some universities in Kyungpook area are selected as a subject of final analysis. The analysis result of internet searching was more meaningful than the analysis result of TV programs or books regarding health. And the older grade(B=.103, p=.007), the more access to health information(B=.137, p=.008) and the higher score of existing health behavior(B=.431, p=.000) the score of improvement of health behavior was increased. Internet searching for health information is the most popular thing because information can be easily get after searching what you want immediately. TV program regarding health has better quality than the information on the internet because of the appearance of health professionals giving health information on TV but it is difficult to watch TV program whenever you want. However the separation between books, TV program and internet is obscured in that the books and TV program can be turned into replay and e-book on internet.