• Korean Journal of Microbiology
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• v.50 no.2
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• pp.137-151
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• 2014

Various studies have been conducted to investigate effects of dams on river ecosystems, but less information is available regarding damming impacts on downstream denitrification. We measured denitrification enzyme activity (potential denitrification rate) and denitrifier abundances (using nirS, nirK, and nosZ as markers) in dammed headstreams of the Nakdong River in South Korea. Sediments in Phragmites-dominated riparian areas and in-stream areas across streams (dammed vs. reference) with different streambed materials (gravel and sand) were sampled occasionally. We hypothesized that (i) the higher available N and C contents in sediments downstream of dams foster larger denitrifier communities than in the reference system and (ii) differences in potential denitrification rates across the systems correspond with denitrifier abundances. Despite 30 years of different hydrological management with dams and greater inorganic N and DOC contents in sediments downstream of dams, compared to the references, abundances of denitrifier communities and potential denitrification rates within the whole sediment were not significantly different across the systems. However, nirS and nosZ denitrifier abundances and potential denitrification rates were considerably increased in specific sediments downstream of dams (gravelly riparian and sandy in-stream) with regard to flooding events and seasonal temperature variation. nirK was not amplified in all sediments. Canonical correspondence analyses (CCA) revealed that the relationship between abundances of denitrifier communities and nutrient availabilities and potential denitrification rates was a weak one.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2001.05a
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• pp.98-99
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• 2001

B화력발전소 3호기의 배가스를 플라즈마를 이용한 탈황/탈질 동시처리기술에 적용하기 위하여 pilot-plant를 설치하고 실 배가스를 이용한 동시처리 실험을 시행하여 배가스 인입온도 $90^{\circ}C$에서 $NH_3$를 $SO_2$와 NOx에 대하여 0.65당량, $C_2$$H_4$를 NOx에 대하여 0.6 당량 주입하고 비전력량을 3～4 Whr/N㎥로 배가스에 인가하면, 탈황율 90%, 탈질율 70% 이상이 얻어지는 것을 확인하였다.

• KSBB Journal
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• v.18 no.6
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• pp.461-465
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• 2003

Five denitrifying bacteria, which were identified as Pseudomonas sp., were isolated by the enrichment culture technique. The most effective denitrifying bacterium was named as Pseudomonas DWS, which was cultivated at anoxic condition. The optimal growth temperature and pH were 30$^{\circ}C$ and 7-8, respectively. Cell growth almost revealed a stationary phase at 18 hours after cultivation and nitrate was degrade 99.9％ during this period. Therefore, it is suggested that Pseudomonas DWS could be effectively used for the biological treatment of wastewater containing nitrogen compounds.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.10
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• pp.949-956
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• 2010

A method for simultaneous removal of nitrogen and phosphate from sewage by elemental sulfur denitrification with membrane bioreactor was proposed, and capacity $10\;m^3$/day of pilot plant was operated for 350 days. This study was investigated to have the effect of denitrification rate and T-P removal with the addition of Alum in Sulfur denitrification Reactor (SDR). The addition of Alum and alkalinity ($NaHCO_3$) in the effluent of MBR was tried to remove simultaneous phosphate and nitrogen in SDR. Characteristics of total nitrogen (T-N) and total phosphate (T-P) removal was compared without and with the addition of Alum as a coagulant. T-N removal without and with the addition of Alum was 92.1% and 87.8%, respectively. And denitrification efficiency was 93.8% and 87.1%, respectively. T-P removal rate was increased to 75.6% in SDR by addition of Alum (2.6~4 mg/L as Al), but T-P removal rate was about 26.7% without the addition of Alum. Therefore, denitrification rate was 6.7% of reduction but T-P removal rate was increased by addition of Alum.

• Microbiology and Biotechnology Letters
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• v.26 no.2
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• pp.96-101
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• 1998

Respiratory denitrification experiments were performed using different carbon sources (acetic acid, glucose, methanol, molasses). In the culture media with glucose and molasses, COD consumption and denitrification rates were higher than with acetic acid and methanol. However, up to 30-40% of reduced nitrate and nitrite were converted to ammonium in glucose and molasses media. In the culture media with acetic acid and methanol, ammonium was not accumulated. Some of the consumed COD seemed to be used by the acid formers for the acidification in glucose and molasses media. By initial pH control of with molasses media, higher denitrification rate (up to 99%) and faster response could be obtained.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2006.11a
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• pp.377-381
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• 2006

실험실 규모의 토양컬럼을 사용하여 도금폐수처리장의 유출수를 대상으로 처리한 결과 다음과 같은 결론을 얻었다. 1) 유입수 $NH_4-N$의 대부분은 질산화 토양컬럼에서 질산화 되었으며 HRT가 단축되면 제거율이 낮아졌다. 2) 질산화 토양컬럼에서 생성된 $NO_3-N$은 탈질 토양컬럼에서 탄소원이 없으면 탈질이 이루어지지 않지만 탄소원으로 메탄올을 주입하면 대부분의 $NO_3-N$는 탈질되었다. 3) 탈질 토양컬럼에서 HRT 72시간일때 T-N 제거율은 81.5%이었고 HRT 48시간일 때는 77.8%이었다. 4) 유입수 평균 T-P농도는 13.06 mg/L이고 탈질 토양컬럼에서의 유출수 평균 T-P는 0.83 mg/L로 제거율은 96.83% 이었다. 5) 실험기간 동안의 COD변화는 탄소원으로 메탄올 주입했 때와 HRT 변화시를 제외하고는 25 mg/L이하로 처리되었으며, 질산화 토양컬럼에는 폐굴껍질을 충진한 결과 $NO_3-N$의 생성에도 불구하고 pH 8.5정도를 유지할 수 있었다.

• Journal of Soil and Groundwater Environment
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• v.11 no.2
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• pp.38-44
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• 2006

Two sulfur-based column reactors inoculated with a bacterial consortium containing autotrophic denitrifiers were operated for 100 and 500 days, respectively and nitrate removal efficiency and the adaptation of microbial communities in the columns were monitored with column depths and time. For better understanding the adaptation phenomenon, molecular techniques including 16S rDNA sequencing and DGGE analysis were employed. Although both columns showed about 99% of nitrate removal efficiency heterotrophic denitrifiers such as Cenibacterium arsenioxidans and Geothrix fermentans were found to a significant portion at the initial stage of the 100-day reactor operation. However, as operation time increased, an autotrophic denitrifier Thiobacillus denitrificans became a dominant bacterial species throughout the column. A similar trend was also observed in the 500-day column. In addition, nitrate removal efficiencies were different with column depths and thus bacterial species with different metabolic activities were found at the corresponding depths. Especially, T. denitrificans was successfully adapted and colonized at the bottom parts of the columns where most nitrate was reduced.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.5
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• pp.542-548
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• 2005

A fluidized bed reactor containing porous media has been known to be effective for nitrogen and organic matters removal in wastewater. The porous media which attached microbes plays important roles in simultaneous nitrification/denitrification (SND) due to coexistence of oxic, anaerobic and anoxic zone. For SND reaction, oxygen and organic substrates should be effectively diffused from wastewater into the intra-carrier zone. However, the overgrowth heterotrophic microbes at the surface of porous media may restrict from substrates diffusion. From these viewpoints, the existence and effect of heterotrophic bacteria at surface of porous media might be the key point for nitrogen removal. A porous media-membrane hybrid process was found to have improved nitrogen removal efficiency, due to stimulated denitrification as well as nitrification. Microelectrode studies revealed that although intra-media denitrification rate in a conventional fluidized bed was limited by organic carbon, this limitation was reduced in the hybrid process, resulting in the increased denitrification rate from 0.5 to $4.2\; mgNO_3-N/L/hr$.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.378-381
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• 2003

The characteristics of bio-beads made of various supports, in which denitrifying bacteria were entrapped after those cells were isolated from sludge in wastewater treatment plants, were studied in order to develop a novel BNR system. Four species were isolated, and the bead made of 12% PVA showed the highest denitrification rate. The best concentration of cell loading was 200 mg/ml, and there was no significant difference in performance by bead sizes. The bead reached the maximum denitrification rate after 4 batch experiments, and with cell leaking of $10^3$ CFU/ml its capacity retained until 25 batches.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.2
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• pp.303-311
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• 2000

This study was conducted to find an optimal TNT transformation condition with the addition of different carbon and energy sources in a batch reactor. When TNT and nitrate were present in the medium, the cell growth and TNT transformation was slower because nitrate and TNT was competitively served as electron acceptor. Transformation of TNT was faster when TNT in the medium was nitrogen source and acetate as a carbon source. Cell growth and nitrate transformation was slower when yeast extract was not present in the medium. The proposed intermediates of TNT biotransformation from the earlier studies was not detected in this experiment but the intermediates are tentatively proposed as nitro and amino-free compounds. These results should be helpful for the operation of the munition waste treatment in the future.