• Korean Journal of Food Science and Technology
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• v.48 no.5
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• pp.454-460
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• 2016

In this study, we investigated the possibility of Droplet digital PCR (ddPCR) for detection of foodborne pathogens. ddPCR combines partitioning of PCR reactions into several thousands or millions of individual droplets in a water-oil emulsion, and counting of positive PCR reaction using flow cytometry. Four species of foodborne pathogens, Bacillus cereus, Staphylococcus aureus, Salmonella Typhimurium and Escherichia coli O157:H7, were used to quantify the target sequence with each of the designed primers and double stranded DNA-binding Evagreen dye. All tested foodborne pathogens showed a detection limit ranging from $100fg/{\mu}L$ to $10ng/{\mu}L$. It was concluded that ddPCR could be used to detect very low concentrations of foodborne pathogens from complex food matrices. For multi-detection of target pathogens, we also tested the samples using multiplex ddPCR and obtained successful results.

• Korean Journal of Food Science and Technology
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• v.50 no.3
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• pp.286-291
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• 2018

This study aimed to investigate the antioxidant ability of a cumin seeds ethanol extract (CE). The DPPH and ABTS radical scavenging activities of 0.25, 0.5, and 1.0 mg/mL of CE were found to be 24.6, 41.4, and 73.4 and 14.5, 27.2, and 50.1%, respectively (p<0.05), suggesting a dose-dependent effect. Moreover, the total phenolic content of CE was $61.0{\mu}M$ tannic acid equivalent/g extract and the FRAP value was $429{\mu}M$ ascorbic acid equivalent/g extract. In 9 hours of oil oxidation, CDA and ${\rho}-AV$ was significantly reduced to 13.4 and 59.1%, respectively, at a CE concentration of 100 ppm compared with that in the control (p<0.05). Major volatile compounds of CE were found to be ${\alpha}$-pinene, 2-butenal, cyclohexene, ${\beta}$-pinene, cis-sabinene, ${\rho}$-cymene, and limonene. These results suggest that CE containing volatile compounds has excellent antioxidant ability and oxidation stability, and thus could be used as a natural antioxidant to prevent oxidation in lipid foods.

• Horticultural Science & Technology
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• v.19 no.2
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• pp.174-178
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• 2001

Absorption potential of oak charcoal was estimated using simulated static and dynamic systems to establish a model for practical application of the charcoal in modified atmosphere (MA) packaging and during the storage of 'Fuji' apples. Practical MA packaging was performed using $60{\mu}m$ PE film zipper bags in which five apples were placed. Absorption potential of oak charcoal was $58.4{\mu}L/100g$ charcoal for ethylene and 583 mg/100 g charcoal for carbon dioxide. Effects of enclosing a 100 g-charcoal packet inside a MA package seemed not to last long enough for quality maintenance of 'Fuji' apples stored at $0^{\circ}C$ for three months. During extended storage, ethylene and $CO_2$ levels were not significantly reduced by charcoal treatment. Nevertheless, absorption of carbon dioxide appeared to alleviate the incidence of $CO_2$-related internal browning disorder. Modelling study of practical storage and marketing procedure indicates that 0.19 kg charcoal/day is required to offset $CO_2$ production from 15 kg of apples at $0^{\circ}C$. The amount of charcoal should be increased to 3.10 kg/day if ethylene is a target gas. From the practical point of view, the results suggest that charcoal could be used only for small unit packages for a short period.

• Journal of Korean Philosophical Society
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• v.123
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• pp.281-307
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• 2012

In the modern society, we have come to recognize human emotions such as anxiety, fear, pain, anger, sadness, longing, desire and pleasure as important topics of philosophy. How shall we study the 'problem of emotions', or in a bigger sense, the 'problem of empathy'? With this critical mind, the research into empathy can be called as a starting point for studying the deepest feelings and thoughts of human beings in connection with the feelings of other people. Most of all, in order to understand other people and to understand human being itself, the role of sympathy or empathy has been emphasized among the problems of emotions, and this trend is especially distinctive in the philosophy of psychology. I definitely acknowledge that empathy or sympathy takes loneliness and agony away from a human being and therefore it is the starting point of communication between people. However, the very fundamental question of 'what are human beings, and who are they?' is once again reminded. In other words, this thesis focuses on the fact that humans are viewed not only as the existence with a soul or heart, but also as a "unique existence" containing something "material-, life- and spiritual", and in that sense, this world is an ecological organism that contains organic connections of humans, material things, plants and animals, which should be the point of view for approach. And it is also emphasized in this study to approach the question about human beings from the religious spirituality point of view that the society with true love for neighbors could be achieved through religious practices that provide principles and value.

• Journal of Korean Philosophical Society
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• v.145
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• pp.245-270
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• 2018

This article aims to examine the possibility of 'philosophy for old age' when humanity begins to reflect on old age in earnest as our country shifts to the aged society. In particular, this article aims to reframe $Ved{\bar{a}}ntic$ philosophy, i.e. a beacon of Hindu philosophy as a model of philosophy for old age, on the assumption that liberation-oriented Hindu philosophies in India can be the most suitable model to frame and apply philosophy for old age mainly at the individual level. First of all, this article identifies that liberation-oriented Hindu philosophies are nothing but a philosophy for the later part of one's life, that is, a philosophy for old age, showing that their common thoughts are very similar to the characteristics of old age. Then, it proves that three points of $Ved{\bar{a}}ntic$ philosophy, i.e. spirit supremacy, the world as an illusion, and freedom from all relations, are fully compatible with philosophy for old age. Finally, it argues that the tradition of Hindu saṃnyāsin (wonderer) embodied to the extreme in $Ved{\bar{a}}ntic$ philosophy is to establish 'old age for philosophy' beyond 'philosophy for old age.' Because $Ved{\bar{a}}ntic$ philosophy establishes saṃnyāsin of old age, which is optimized to strive for the truth as the confident subject of philosophical inquiry, so much that it exists more 'by old age' than 'for old age.' After all, according to $Ved{\bar{a}}ntic$ philosophy, as ontological and practical positivity is maximized in old age, so old age itself can be the best position to overcome not 'negativity of old age', but 'negativity of human condition' or 'negativity of life.'

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.49-49
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• 2019

국립수목원은 열대유용식물의 BT산업 원천소재 확보 제공을 목적으로 열대자원연구센터를 건립하여, 열대 및 아열대 전시원을 관리하고 있다. 온실의 특성상 일반 재배환경과는 다르게 여러 종류의 식물의 혼합 식재되어 있는 특성이 있어서 농업환경과 달리 해충의 급격한 돌발에 의한 온실 전체적인 피해의 위험성이 적은 반면에 안정된 환경으로 인해서 해충의 취약한 몇몇 수목의 경우 해충이 급격히 늘어날 수 있다. 본 실험은 여러 종류의 식물이 혼재되어 있는 수목 온실 내에서 생물적 방제를 이용하여 주요 해충류인 진딧물류 및 가루 깍지벌레, 잎응애류 방제 가능성을 타진하고자 진행되었다. 진딧물이 집중적으로 발견된 열대식물 5종(Acalypha hispida, Justicia gendarussa, Hamelia patens, Ficus petersii, Hibiscus rosa-sinensis)을 중심으로 천적 방사 및 Banker plant 설치를 진행했다. 최초 조사일인 2015년 3월 10일, Tropical 1에 진딧물류의 발생이 많이 확인되어 포식성 천적인 미끌애꽃노린재를 투입했다. Tropical 2는 2015년 3월 17일 기생성 천적인 콜레마니진디벌과 선발된 Banker plant를 적용하여 방제 되었다. 철모깍지벌레가 발견된 열대식물 3종(Sanchezia parvibracteata, Brillantaisia lamium, Pseuderanthemum atropurpureum)을 중심으로 적용 천적으로 어리줄풀잠자리, 애홍점박이 무당벌레와 깍지무당벌레를 선별했다. 천적을 대상으로 철모깍지벌레에 대한 포식능력을 조사했으며 애홍점박이 무당벌레가 어리줄풀잠자리 보다 2.8배, 깍지무당벌레보다 2.9배 포식능력이 뛰어남을 확인했다. 잎응애류가 발견된 열대식물 2종(Acalypha wilkesiana, Erythrina caffra)은 천적으로 사막이리응애와 칠레이리응애를 적용하였다. 괭이밥응애 성충과 알의 포식력을 검증한 결과 사막이리응애가 괭이밥응애를 먹이로 증식이 가능한 것으로 확인되었다. 괭이밥, 괭이밥응애, 사막이리응애를 효과적으로 조합한 응애류 방제를 위한 새로운 Banker plant의 개발 가능성을 확인할 수 있었다. 연구는 열대온실의 열대식물 관리에 대한 생물학적 방제의 지속적인 모니터링을 통해 종합적 방제의 관리 자료로 이용되어 새로이 온실을 조성하는 경우 식재기반조성에 실질적인 도움을 줄 수 있으며, 추후 열대식물의 자원 식물로서의 활용성을 높일 수 있다.

• Food Science and Preservation
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• v.25 no.1
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• pp.98-106
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• 2018

Portulaca oleracea L., a species of Portulacaceae, is ubiquitous. It is a well-known traditional Chinese medicine for removing heat, counteracting toxicity, cooling blood, and maintaining hemostasia; it is also used as antidysentery agent. This study investigated the anti-oxidative and anti-inflammatory activities of water and ethanol extracts from P. oleracea. The total polyphenol content ($21.08{\pm}0.03mg\;GAE/g$) and total flavonoid content ($5.45{\pm}0.76mg\;QE/g$) of the ethanolic extracts were higher than those of the water extracts. The antioxidative activities were determined by evaluating the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activity and by the ferric reducing antioxidant potential (FRAP) assay. The ABTS radical scavenging activity of the water extract (75.53%) was higher in those of the water extract (67.03%) at concentration of $1,000{\mu}g/mL$. The DPPH radical scavenging activity and FRAP of the ethanol extract were higher than those of the water extract. We also investigated the anti-inflammatory activity of the P. oleracea extracts in LPS-stimulated Raw 264.7 cells. The production levels of nitric oxide (NO) and reactive oxygen species (ROS) significantly decreased with an increasing concentration of the extract. The expression levels of pro-inflammatory cytokines (tumor necrosis faction (TNF)-${\alpha}$, interleukin (IL)-$1{\beta}$, and IL-6) were significantly lower in the ethanol extract than in the LPS alone treatment group. Based on these results, ethanolic extract from P. oleracea could be an effective antioxidant and anti-inflammatory agent.

• Korea Science and Art Forum
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• v.37 no.2
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• pp.101-112
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• 2019

Regardless of the size, the role and meaning of the space required for human daily life are the same. Especially, as the spatial design is small and the size is small, the careful design and problem solving are needed to enable comfortable and convenient life even in a narrow space. The purpose of this study is to design a convergence model that utilizes the advantages of BIM, which can simulate actual design based on a new one-person housing space design plan optimized for one person. This study applied the BIM design technology to one-person housing 2D design, and the suitability examination and the space optimization design of the interior design were carried out. As a result of the study, utility of space improvement, consideration of housing environment, interference check, application of eco-friendly housing system, and MEP design item were derived. Therefore, BIM space design in interior space has been confirmed as a way to overcome limit and inefficiency of 2D design which is applied to actual space by various space design elements. Based on the results of this study, the One-person housing space model, which is applied to the study, is a pure creation designed based on various one-person housing of social and cultural peculiarities derived from previous research. This design example was applied to BIM technology to confirm the detailed and practical design possibility.

• The Korean Journal of Archival Studies
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• no.68
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• pp.205-241
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• 2021

This article explores how to incorporate the topic of the global environmental crisis called the "anthropocene" into archives studies and connect it to ecological practical reasons. In order to encourage discussion of archival studies, which puts the environmental crisis at a kind of archive constant value, this study seeks to examine the possibility of a quality shift in archival studies based on ecology. This article aims to go beyond the pragmatism of preparing improvements to eco-friendly record management, which is recently claimed by the "Green Archive" in Western archival studies. It calls for a new concept called 'ecological archive', which theoretically combines a more archives-based and ecological paradigm, and its epistemological transformation. Specifically, the ecological approach of archives is first discovered in the discussion of archival studies and at the same time, through the "ecological turn" of archives emphasized by recent anthropocene discourses, the concept of "ecological archive" emphasized by this article is embodied. This study uses 'ecological archive' as a universal and theoretical framework for archives as a basic concept for building ecological 'living' archives. In other words, for the construction of ecological archives, we reinterpret and extend so-called democratic values for archives, i.e., post-custodianship, community archives, and archives of emotions. Finally, the records of foot-and-mouth disease killing burial sites, an important site and example of the anthropocene tragedy, exemplifies the methodology of the actual application of ecological living archives. The case analysis aims to seek a new qualitative shift in record management that adapts to global ecological transformation, while also emphasizing the documentation by archival activism in ecological field practices jointly organized by archivists and citizens.

• Cross-Cultural Studies
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• v.48
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• pp.325-359
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• 2017

The works of a literary and cultural historian Franco Moretti are conspicuous in many ways. Trained in Marxism and Russian formalism, he participated in the construction of the New Left in England. Also, he interestingly interpreted the socialization of the individual through the genre of bildungsroman. Then, he shifted his research interest to the notion of world literature, and to explore its global scale, he developed his own quantitative approach combined with advanced computer technology in digital humanities. His recent publication reveals that Moretti conducted a social critique of the European bourgeois culture with his new quantitative method. His macroscopic view of literature and use of cutting-edge technology in his research inspire historians of Korean literature located in the so-called periphery of world literature. Therefore, the purpose of this study is to examine the idea of world literature outlined by Franco Moretti by reviewing his method called "distant reading" and examples of such an approach. His distant reading is to construct a macroscopic archive through inclusion of forgotten works from literary history and to analyze morphological patterns that frequently appear in the archive. His book entitled Graphs, Maps, Trees is a collection of examples of which he applied distant reading. By delving into such cases, I will raise questions about Moretti's macroscopic perspective of world literature in conjunction with Korean literature. As located at the periphery of global circulation of literary knowledge, Korea appropriated Western genres, established its literary institutions, and developed book markets through modern newspapers and magazines. This experience of furthering modern literature through periodicals would provide another view to revisit Moretti's world literature.