• Korean Journal of Remote Sensing
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• v.36 no.2_2
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• pp.309-323
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• 2020

Estimation of chlorophyll a concentration (CHL) on coastal areas using remote sensing has been mostly performed through multi-spectral satellite image analysis. Recently, various studies using hyperspectral imagery have been attempted. In particular, airborne hyperspectral imagery is composed of hundreds of bands with a narrow band width and high spatial resolution, and thus may be more effective in coastal areas than estimation of CHL through conventional satellite image. In this study, comparative analysis of hyperspectral and satellite-based CHL images was performed to estimate CHL in coastal areas. As a result of analyzing CHL and seawater spectrum data obtained by field survey conducted on the south coast of Korea, the seawater spectrum with high CHL peaked near the wavelength bands of 570 and 680 nm. Using this spectral feature, a new band ratio of 570 / 490 nm for estimating CHL was proposed. Through regression analysis between band ratio and the measured CHL were generated new CHL empirical formula. Validation of new empirical formula using the measured CHL showed valid results, with R² of 0.70, RMSE of 2.43 mg m^-3, and mean bias of 3.46 mg m^-3. As a result of applying the new empirical formula to hyperspectral and satellite images, the average RMSE between hyperspectral imagery and the measured CHL was 0.12 mg m^-3, making it possible to estimate CHL with higher accuracy than multi-spectral satellite images. Through these results, it is expected that it is possible to provide more accurate and precise spatial distribution information of CHL in coastal areas by utilizing hyperspectral imagery.

• Restorative Dentistry and Endodontics
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• v.27 no.6
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• pp.600-611
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• 2002

본 연구의 목적은 클로로필린이 치주인대 세포 생활력에 미치는 영향을 평가하는데 있다. 치주인대 세포는 건강한 사람에서 발치한 소구치의 치주인대 조직으로부터 채취하여 배양하였다. 비교 기준을 설정하기 위하여, HBSS 내에서 $37^{\ circ}C$로 보관한 치주인대 세포수의 50%가 생존하는데 소요되는 시간을 MTT 분석법에 의해 측정하였다. 그 결과는 6시간이었다. HBSS에 클로로필린 10, 100, 500 nM이 각각 첨가된 3군의 실험 보존액과 F-medium, ViaSpan, Likorol 등 모두 6군의 실험 보존액을 96-well plate에 접종한 후, 치주인대 세포를 동일한 수로 분주하였다. 이를 6시간동안 보관한 후, 각 실험군 보존액에서의 세포 생활력을 MTT 분석법으로 측정하였다 또한, HBSS와 F-medium 및 클로로필린 500 nM이 첨가된 HBSS군의 세포들에 대해 유식세포 계측을 시행하여 각각의 세포주기를 분석하였다. 실험 결과, 클로로필린 500 nM이 첨가된 HBSS에서 보관한 세포들이 가장 높은 생활력을 나타내었으며, 클로로필린이 첨가되지 않은 HBSS에 비해서 유의하게 양호한 세포 생활력 유지 능력을 보였다. 클로로필린으로 처치한 세포들은 클로로필린의 농도가 커짐에 따라 세포 생활력이 증가하는 양상을 나타내었다. 유식세포 계측 결과, HBSS와 F-medium 및 클로로필린 500 nM이 첨가된 HBSS에서 보관한 세포의 77~80%가 G0-G1 단계의 세포 주기로 측정되어, 대부분의 세포들이 안정된 세포 대사 상태를 보이는 것으로 나타났다. 결론적으로, 클로로필린 처치는 치주인대 세포의 생활력 유지에 도움이 되는 것으로 사료된다.

• Applied Biological Chemistry
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• v.44 no.2
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• pp.73-80
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• 2001

Factors such as temperature $(20,\;60^{\circ}C)$), pH (4.5, 7.0), gaseous phase $(N_2,\;0_2)$, and light (0 lux, 5,000 lux), antioxidants and packaging conditions were investigated to study the effects of above factors on the chlorophyll components in spinach during storage. Regardless of other factors, as the storage temperature increased from $20^{\circ}C$ to $60^{\circ}C$ and pH decreased from 7.0 to 4.5, the contents of chlorophyll a and chlorophyll b in spinach decreased significantly (P<0.05). The amounts of chlorophyll a and chlorophyll b in spinach stored in nitrogen gas were significantly (P<0.05) lower than those in sample in oxygen phase. As the light intensity increased from 0 lux to 5,000 lux during storage, the contents of chlorophyll a and chlorophyll b in spinach significantly (P<0.05) decreased. The antioxidants reduced the degradation of chlorophyll a in a model system during dark storage by minimization of free radical oxidation. The effectiveness of antioxidants decreased as following orders; ${\alpha}-tocopherol$>ascorbic acid>${\beta}-carotene$>catechin>quercetin>rutin>kaempherol>caffeic acid>chlorogenic acid>p-coumaric acid>ferulic acid. The degradation of chlorophyll a in a model system during light storage was minimized by antioxidants due to the reduction of singlet oxygen oxidation. The antidiscoloring potential of antioxidants decreased as following orders; ${\beta}-carotene$>${\alpha}-tocopherol$>ascorbic acid>catechin>quercetin>rutin>kaem-pherol>caffeic acid>chlorogenic acid>p-coumaric acid>ferulic acid. The amounts of chlorophyll a and chlorophyll b in freeze dried spinach packed with polyethylene bag were significantly (P<0.05) lower than those in non-packed freeze dried spinach. The package of spinach in polyethylene bag with the combination of antioxidants could be used to minimize the degradation of chlorophyll components in spinach during storage.

• Korean Journal of Food Science and Technology
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• v.41 no.6
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• pp.700-705
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• 2009

Several studies have demonstrated that chlorophyll has many beneficial properties, including anti-inflammatory, anti-tumor, and antioxidant properties. Chlorophyll has been also shown to have an excellent chemopreventive potential against skin tumor. Its preventive mechanism against skin tumor, however, has not been examined in detail. Furthermore, the effects of chlorophyll on UVB-induced cellular responses have not been investigated. Solar UV radiation, in particular its UVB component, is the primary cause of many adverse biological effects, which is responsible for the photoaging and skin cancer. We investigated the preventive effects of chlorophyll a on UVB-mediated responses in human immortalized HaCaT kerationocytes and normal human fibroblast. We found that treatment of chlorophyll a markedly inhibited UVB-induced generation of reactive oxygen species and lipid peroxidation. Chlorophyll a also prevented UVB-induced MMP-1 expression and MMP-2 activation and increased Type I pN collagen synthesis. These results suggest that chlorophyll a is a potent candidate for the prevention and treatment of UVB-induced skin cancer and photoaging.

• Korean journal of food and cookery science
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• v.21 no.3 s.87
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• pp.339-345
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• 2005

This study was carried out to determine the changes of chlorophyll contents in spinach during growth stages and storage with two packaging materials. The chlorophyll contents increased to a maximum level in the spinach leaves after 35 days of planting, while the level in the roots extremely decreased and that in the stems did not change during the growth period. The total chlorophyll content was higher in the leaves than that in the stems and roots. The ratios of chlorophyll a/b in the leaves and stems were almost similar to those reported for spinach elsewhere. When stored for 1, 3, and 5 days without packaging, the remaining percentage of chlorophyll was $96.29\%,\;90.79\%\;and\;85.74\%$, respectively, at $5^{\circ}C,\;and\;82.75,\;44.63,\;33.07\%$, respectively, at $20^{\circ}C$. The chlorophyll contents of the spinach stored in polyethylene bags were significantly higher than of that those without packaging and of that packaged with newspaper during storage. From the results, it was found that spinach packaged in polyethylene bags suppressed the degradation of chlorophyll and maintained the freshness during low temperature storage and that the chlorophyll contents increased during the growth period.

• Journal of the East Asian Society of Dietary Life
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• v.13 no.6
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• pp.586-592
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• 2003

This research used High Performance Liquid Chromatography(HPLC) to analyze and compare the content and the pattern of chlorophyll to find the difference in quality, water temperature, and the stirring time between the three different types of Korean and Japanese powder green tea, which were currently on the market. Chlorophyll contents of Japanese-made medium quality tea (S5: 8.34mg/g), and low quality tea (S6: 8.23mg/g) were relatively high, and that of Korean-made medium quality tea (S2 ; 4.87mg/g) was lower. The water temperature of 85$^{\circ}C$ and 95$^{\circ}C$ were high except in Korean-made medium and low quality powder green tea (S2 and S3). Japanese-made powder tea required 60 seconds of stirring time and Korean-made tea required 15 seconds, but 30 seconds seem to be the ideal stirring time for both of them. Chlorophyll a, b, pheophytin a, b, and pheophorbide were confirmed from the chlorophyll pattern. There were 1.5∼2 times more of chlorophyll a in Japanese-made one compared to that of Korean-made one; also 1.5∼2 times more of pheophytin a in Korean-made one than Japanese-made one. It might be concluded that Japanese-made powder green tea was higher in quality.

• Korean Journal of Remote Sensing
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• v.33 no.6_1
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• pp.917-930
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• 2017

To understand the temporal and spatial variations of surface chlorophyll-a concentration (Chl-a) distribution in the Indian Ocean ($30^{\circ}E{\sim}120^{\circ}E$, $30^{\circ}S{\sim}30^{\circ}N$) by the Indian Ocean Dipole (IOD), we conducted EOF and K means analyses of monthly satellite-derived Chl-a data in the region during 1998~2016 periods. Chl-a showed low values in the central region of the Indian Ocean and relatively high values in the upwelling region and around the marginal regions of the Indian Ocean. It also had a strong seasonal variation of Chl-a, showing the lowest value in the spring and the highest value in summer due to the change of the monsoon and current system. The EOF analysis showed that Chl-a variation in EOF mode 1 is related to ENSO (El $Ni{\tilde{n}}o$/Southern Oscillation) and that of mode 2 is linked to IOD. Both modes explained spatially opposite trends of Chl-a in the east and west Indian Ocean. From K means analysis, the Chl-a variation in the east and west Indian Ocean, and around India have relatively good relationship with IOD while that in the tropical and middle Indian Ocean closely associated with ENSO. The spatial and temporal distribution of Chl-a also showed distinct spatial and temporal variations depend on the different types of IOD events. IOD classifies two patterns, which occurred during the developing ENSO (First Type IOD) and the year following ENSO event (Second Type IOD). Chl-a variation in the First Type IOD started in summer and peaked in fall around the east and west Indian Ocean. Chl-a variation in the Second Type IOD occurred started in spring, peaked in summer and fall, and disappeared in winter. In the Chl-a variation related to IOD, developing process appearing in the Chl-a difference between the east and west Indian ocean was similar. Chl-a variation in the northern Indian Ocean were opposite trend with changing developing phase of IOD.

• Proceedings of the KSRS Conference
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• 2008.03a
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• pp.170-175
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• 2008

본 연구는 MODIS에서 제공하는 클로로필 정보를 기반으로 하여 2단계 필터링을 통해 우리나라 동해, 남해 연안에 대규모로 발생했던 Cochlodinium polykrikoides 적조를 탐지하는 알고리즘을 제시한다. 일반적인 적조 탐지 연구들은 클로로필과 적조 발생의 상관성을 이용하여 클로로필의 농도가 높은 해역을 적조 발생 해역으로 탐지한다. 하지만 이 방법의 문제점은 적조가 발생하지 않은 해역을 적조 발생 해역으로 탐지함으로써 commission error를 발생시킨다는 것이다. 따라서 본 연구에서는 이러한 문제점을 극복하기 위해 MODIS에서 제공하는 클로로필 정보를 바탕으로 적조 발생 해역을 추출하고, 2단계 필터링 과정을 적용함으로써 진해, 여수, 남해도 부근 해역에서 발생한 commission error를 제거할 수 있었으며, 그 결과를 국립수산과학원의 적조속보자료와 함께 시각적 평가하여 본 연구에서 제안한 알고리즘의 효용성을 검증하였다. 향후 정량적인 평가를 위해 F-measure, JC(Jaccard coefficient), YC(Yule coefficient), 전체정확도를 탐지정확도 측정치로써 도입하여 정확도평가를 수행할 예정이다.

• Proceedings of the KSRS Conference
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• 2009.03a
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• pp.337-343
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• 2009

1997년 9월부터 2007년 12월까지 인공위성 해색센서 SeaWiFS의 클로로필-$\alpha$ 농도가 가지는 오차 특성을 분석하고 그 원인을 조사하였다. 동해의 클로로필-$\alpha$ 월별 분포에는 비정상적으로 높은 농도값이 스펙클(speckle) 형태로 출현하였다. 스펙클들은 시공간적으로 연계성이 없이 산발적으로 분포하였으며 주변 평균에 대해 $10mg/m^3$ 이상의 편차를 보였다. 스펙클들은 주로 겨울철에 나타났으며 구름 분포와 관련이 있었다. 10년간 월별 운량 분석 결과 겨울철 운량은 다른 계절과 달리 남동해상에 집중적으로 분포하였으며, 운량이 클수록 스펙클의 농도가 크게 나타나는 통계적 특성을 보였다. 특히 스펙클이 나타나는 화소의 각 밴드별 정규화된 수출광량을 분석해 본 결과 짧은 파장 영역(443, 490, 510 nm)은 전체적으로 수출광량이 낮게 나타난 반면 550 nm 밴드는 정상 화소와 유사한 분포를 보였다. 짧은 파장 영역의 낮은 수출광량은 555 nm 밴드에 대한 비율로 구해지는 클로로필-$\alpha$ 농도값을 비정상적으로 증폭시켰으며 SeaWiFS 자료에 스펙클을 유발하였다. 본 연구는 동해의 SeaWiFS 클로로필-$\alpha$ 농도자료가 지니는 스펙클 오차에 대한 문제점을 제기하고 오차 특성 분석을 통해 좀 더 신뢰도 있는 자료를 해양 응용 연구에 사용해야 함을 제시한다.

• Korean Journal of Remote Sensing
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• v.18 no.4
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• pp.187-199
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• 2002

Correlation between chlorophyll a in the East China Sea and spectral bands (412, 443, 490, (510), 555, (676, 765)nm) of Ocean Scanning Multi-Spectral Imager (OSMI) including the profile multi-spectral radiometer (PRR-800) was studied. The values of remote sensing reflectance (Rrs) at the bands corresponding to the field chlorophyll $\alpha$ in the East China Sea were much higher than those in clear waters off California, USA. In case of the particle absorptions related to the chlorophyll a concentration at the spectral bands (440, 670nm) were much higher in the East China Sea than the ones in the clean waters off California. The normalized water leaving radiances (nLw) at 412, 443, 490, 555 nm of OSMI and the field chlorophyll a in the East China Sea were correlated each other. According to the results, the relationship between field chlorophyll $\alpha$ and nLw 410 nm in OSMI bands was the lowest, whereas that between field chlorophyll a and nLw 555 nm in the bands was the highest. Reciprocal action between the field chlorophyll a and the band ratio of the OSMI bands (nLw410/nLw555, nLw443/nLw555, nLw490/nLw555) was also studied. Relationship between the chlorophyll $\alpha$ and the band ratio (nLw490/nLw555) was highest in the OSMI bands. Relationship between the chlorophyll $\alpha$ and the ratio (nLw490/nLw555) was higher than one in the nLw410/nLw555. The difference in the estimated chlorophyll $\alpha$ (mg/m$^3$) between OSMI and SeaWiFS (Sea Viewing Wide Field-of-View Sensor) at the special observing stations in the northern eastern sea of Jeju Island in February 25, 2002 was about less than 0.3 mg/m$^3$ within 3 hours. It is suggested that OC2 (ocean color chlorophyll 2 algorithm) be used to get much better estimation of chlorophyll $\alpha$ from OSMI than the ones from the updated algorithms as OC4.