• The Korean Journal of Mycology
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• v.47 no.3
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• pp.259-269
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• 2019

Brown spot and sheath rot of rice are caused by fungal pathogens such as Curvularia lunata, Cochliobolus miyabeanus, and Sarocladium oryzae, and cause losses such as reduced rice yield and quality, which is an enormous problem with serious long-term effects. To search biological control agents of phytopathogenic fungi, five kinds of useful Bacillus-like isolates which are excellent in extracellular enzyme activity and produce siderophore were selected from paddy soil of Sunchang in Korea. The selected isolates were tested for excellent antifungal activity against three of the phytopathogenic fungi that frequently occur in rice, and JSRB 177 strain had the most excellent antifungal activity. Based on the experimental results, JSRB 177 is finally selected as a candidate for biological control and identified to Bacillus subtilis through 16S rRNA sequence analysis. In addition, physiological characteristics of JSRB 177 confirmed by analysis of carbohydrate fermentation patterns and enzyme production ability. Based on the above results, JSRB 177 is expected to be used as a biological control agent for the rice pathogenic fungi. In the future, further studies related to industrialization such as port test and establishment of mass production process are needed.

• The Journal of Natural Sciences
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• v.7
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• pp.103-114
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• 1995

These experiments were conducted for decrease of injury by continuous cropping in the peanut fields of Chonbuk Wangkungarea. The continuous cropping field for four years was used in this experiment. Italian ryegrass and rye were cultivated andlime materials were distributed for improvement of soil fertility. The results were as follows; 1. Forage crops were cultivatedand lime materials were distributed on the continuous cropping field of peanut. The organic matter content of the expermentalplot cultivating Italian ryegrass was only 1.25%. The organic matter content of soil cultivated Italian ryegrass after distributedmagnesium lime was 1.37% and that of soil cultivated Italian ryegrass after distributed gypsum was 1.30%. It was highcontent comparing to that of soil distributed lime materials only. The organic matter content of soil cultivated rye after distributed gypsum was 1.77%. 2. The phosphate content of soil cutivated Italian ryegrass was 332ppm. The phosphate content ofsoil cultivated Italian ryegrass after distributed magnesium lime was 34Oppm and that of soil cultivated Italian ryegrass afterdistributed gypsum was 31 2ppm. The phosphate content of soil cultivated rye only was 386ppm. The phosphate content ofsoil cultivated rye after distributed gypsum was 41 8ppm. This phosphate content was lower than that of soil distributed limematerials only. 3. The phytotoxin content of soil cultivated Italian ryegrass after distributed magnesium lime was decreased to17.7% and that of soil cultivated Italian ryegrass after distributed gypsum was decreased to 25.3%. The phytotoxin content ofsoil cultivated rye after distributed magnesium lime was decreased to 12.0% and that of soil cultivated rye after distributedgypsum was decreased to 12.8% comparing to the phytotoxin content of soil distributed lime materials only. Italian ryegrasswas effective to decrease phytotoxin among the forage crops and gypsum was effective among the lime materials. 4. Abacterial wilt and a late spot of peanut which were known as, main reason of continuous cropping failure were surveyed.lnccidence of a bacterial wilt was 3.4% in the plot cultivated Italian ryegrass only and that was 2.9% in the plot cultivated ryeonly. lnccidence of a bacterial wilt was 2.5% in the plot cultivated Italian ryegrass after distributed magnesium lime and thatwas 2.3% in the plot cultivated rye after distributed gypsum. Inccidence plot cultivated forage crops was lower than that of plotdistributed lime materials. 5. Inccidence of a late spot was high in the plot cultivated forage crops ony, but it was low in the plotcultivated forage crops after distributed lime materials comparing to that of the control plot. 6. The growth and yield of peanutwere bad in the plot cultivated forage crops only comparing to the control plot distributed lime materials only. These resultswere same in the plot cultivated rye after distributed lime materials, but the growth and yield were grown up in the plotcultured Italian ryegrass after distributed lime materials.

• Korean journal of applied entomology
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• v.16 no.1 s.30
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• pp.13-19
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• 1977

The smaller brown planthopper overwinters mostly as the fourth instar nymph. The emerged adult disperses to winter barley or wheat plants. The adult of the first generation disperses into the rice field, and spends three generations in the rice field. The population densities of the adults of the first generation and the overall density of the second generations are the most important in relation to the incidents of the rice stripe disease which is transmitted by this insects. This study was intended to analyse the population increases in these important generations in relation to the kind and the different growing stage of the host plants. The insects were reared on potted host plants of different growing stage in the insect cages. The population increases of the insects in the cages were compared with those on the rice seedlings in the laboratory. The results are as follows: 1. The average longevities of the adults of the generations were 13.4 days for the overwintered, 12.9 days for the first, and 10.2 days for the second generation, and showed no significant differences between the generations even they seemed to be shorter than normal condition probably caused by the frequent disturbance at the time of food renewal in 2 or 3 days. 2. When they were reared on the rice seedling, the average numbers of the eggs per female were 131.2 for the overwintered generation, 124.9 for the first generation grown on the barley, and 142.3 for the second generation grown on rice, and shelved no significant differences between the generations. More than $90\%$ of the eggs were laid within 3 weeks. 3. The hatchabilities of the eggs laid on the rice seedling in laboratory were $77.4\%$ for the first generation, $93.1\%$ for the second generation and $62.1\%$ for the third generation. The estimated hatchabilities of the eggs were $67.5\%$ for the first generation, $84.1\%$for the second generation, and $21.9\%$ for the third generation with the growing plants on the plots. The hatchability on the eggs laid by the adults infested on the barley on May 17 was $13.5\%$ compared with $67.5\%$ for the eggs laid by those infested on barley in late April. 4. The average total nymphal periods were 19.4 days for the first generation, and 13.3 days for the second generation in the laboratory and the mortalities of the nymphs seemed to related with the growing stages of the host plants. 5. With the pot experiments, the average per cents of the emerged adults were $37.2\%$ for the first generation, $55.3\%$ for the second generation, and $11.6\%$, for the third generation. The average numbers of the emerged adults were $7.4\%$ for the pots infested on the barley on May 17, instead of $37.2\%$ adults for those infested in late April. 6. From above results, it may suggest that the innate qualities of the insect are not significantly different between generations, and the differences in the increase of the population for the generations might effected considerably by the differences in the physiological conditions of tile host plant, besides the differences in physical and other biological factors.

• Korean Journal of Plant Resources
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• v.21 no.1
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• pp.73-82
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• 2008

The resource plants of the Mt. Geonji was listed 354 taxa; 92 families, 242 genera, 303 species, 48 varieties and 3 forms. 354 taxa listed consists of 205 taxa of edible plants(57.1%),234 taxa of medicinal plants(65.2%), 167 taxa of ornamental plants(46.5%) and 218 taxa of the others(60.7%). Specific plant species by floral region were total 22 taxa; Trapella sinensis var. antennifera in class IV, Iris ensata var. spontanea in Class II, 16 taxa(Salix glandulosa, Alnus hirsuta, Chrysosplenium flagelliferum, Mallotus japonicus, Ilex macropoda, Grewia biloba var. parviflora, Vaccinium oldhami, Lysimachia barystachys, Fraxinus mandshurica, etc.) in class I. The naturalized plants in this site were 12 families, 23 genera, 28 species, 2 varieties, 30 taxa(Bromus unioloides, Phytolacca americana, Oenothera erythrosepala, Ipomoea hederacea var. hederacea, Aster pilosus, Erechtites hieracifolia) and naturalization rate was 8.5% of all 354 taxa vascular plants. Wild plants disturbing ecosystem like Solanum carolinense and Ambrosia artemisiifolia var. elatior have been increasing. So, it needs continuing control and conservation measures on the plant ecosystem.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.4
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• pp.283-288
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• 2013

To evaluate the ability of the submerged plant, Ceratophyllum demersum's (C. demersum) to remove nutrients and to inhibit growth of cyanobacteria, a total of 6 mesocosms were conducted in a batch reactor for 9 days. From the 84 hr of the experiment, C. demersum was stabilized and showed daily cycle trends according to changes in pH and DO levels. The concentration of nutrients, $NH_3{^+}$, $NO_3{^-}$ and $PO_4{^3}$ continuously decreased until 9 days of the experiment, with the rapid decrease in nutrient concentration for the first 24 hours. High correlation coefficient ($r^2{\geq}0.96$, p<0.001) between the amount of C. demersum's biomass per unit area and the nutrients removal level were derived, and greater C. demersum's biomass per unit area showed higher removal efficiency of nutrients. However, there were differences in the C. demersum's activity level between batch reactors with higher and similar density of the C. demersum, but nonetheless water purification effect appears to have a significant influence due to attached algae and microorganisms. The growth rate of harmful cyanobacteria, Microcystis aeruginosa (M. aeruginosa) with C. demersum's density of 2,500 g $fw/m^2$ (100% of cover degree) was 0.31 /day, compared to the growth rate of 0.47 /day for the control group (0% of cover degree). In terms of number of cells, the control group had 1.7 times higher number of cells than the experimental group, proving that C. demersum has the ability to inhibit the growth of harmful cyanobacteria.

• Korean Journal of Weed Science
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• v.9 no.1
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• pp.46-55
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• 1989

Stomatal variation was observed at the Yeongnam Crop Experiment Station in 1988 using 42 rice cultivars and 30 weed species. The shape, density or size of stomata was varied depending on the species. Two general trends, however, were found that more number of stomata was found at lower leaf epidermis than upper leaf epidermis and stomata number was negatively correlated with stomata size. Aneilema japonica and Portulaca oleracea had the least number of stomata having 17-20 stomata per $m^2$ for upper leaf epidermis and 17-54 stomata for lower leaf epidermis while Polygonum conspicuum had the greatest number of stomata (449 for upper leaf epidermis and 511 for lower leaf epidermis). Soybean, Aeschynomene indica, Ludwigia prostrata and Lactuca indica had the smallest in stomata size while the biggest stomata was found at P. oleracea and A. Japonica that had the least number of stomata. Cyperus species such as C. difformis, C. iria and C. serotinus had no stomata at upper leaf epidermis. The stomata were distributed only at lower leaf epidermis for these species. Potamogeton distinctus, on the other hand, had stomata almost at upper leaf epidermis and thus, hardly found the stomata at lower leaf epidermis. Among rice cultivars, Tongil-type had the greatest number of stomata followed by Indica-type and Japonica-type, in order. Cultivars released after 1960 had more stomata than cultivars released before 1960 for Japonica-type cultivars while stomata size had reversed trend. Jinheung had the least number of stomata (${\fallingdotseq}$ 150 per $mm^2$) while Yushin had the greatest number of stomata (350 for upper and 449 for lower leaf epidermis, respectively) among rice cultivars. Other cultivars having more than 350 stomata per $mm^2$ were Samgangbyeo, Milyang 23, Woonbongbyeo, etc.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.2
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• pp.300-307
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• 1997

The effects of Aster scaber and Ixeris dentata on cadiovascular system in hyperlipidemic rats were examined. Five groups of thirty Sprague Dawley rats were fed with the diet contained 1% cholesterol, 0.25% sodium cholate, 10% coconut oil and 5% lard(control group) for 4 weeks. Each experimental diet group was added with 5% plant powder or extract of the 5% plant powder by dry weight. Contractile or relaxation responses in the isolated artria and thoracic aortae were measured and the morphological changes of the aortic endotherium from the rats fed the experimental diet were inspected. In response to isoproterenol, the number of right atrial spontaneous beat was significantly lower in Cham chyi powder group$(PP_{1})$ and Sumbagui powder group$(PP_{2})$ than control at $10^(-8)M$ concentration. The contraction forces by injection of phenylephrine and calcium in isolated thoracic aorta were significantly low in each experimental groups compared with the control. The relaxation rates by acetylcholine represented comparatively higher value in $PP_{1}$ than control. The morphological changes of endothelial cell surface was a little in $PP_{1}$ and $PP_{2}$ compared with control, while the damages were considerably advanced in Cham chyi and Sumgbagui extract diet group$(PE_{1},\;PE_{2})$.

• Sleep Medicine and Psychophysiology
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• v.13 no.1
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• pp.27-32
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• 2006

Objectives: Nasal continuous positive airway pressure (nCPAP) is the treatment of choice and has been shown to reduce the frequency of nocturnal respiratory events, improve sleep architecture, and decrease daytime sleepiness in patients with obstructive sleep apnea syndrome (OSAS). However, little is known about the compliance of nCPAP treatment in Korea. Our objective was to look into the nCPAP compliance and examine the factors influencing it. Methods: We reviewed the records of one hundred and twenty consecutive patients with OSAS referred for nocturnal polysomnography with nCPAP pressure titration during the period of January 1995 through April 1999 to the Seoul National University Hospital. We performed a telephone interview and obtained data from eighty-three patients. Results: In sixty patients who had accepted nCPAP treatment, twenty-six patients (43.3%) were still using nCPAP device, while thirty-four patients (56.7%) stopped using it. Fifteen patients (25%) were using nCPAP device everyday. In thirty-four patients who discontinued nCPAP use, twenty-five patients (73.5%) did within the first three months, and thirty-one (91.2%) within the first year. Significant predictor of long-term nCPAP use was the presence of subjective daytime sleepiness before nCPAP application. Conclusions: Long-term compliance with nCPAP treatment appears to be associated with the presence of subjective daytime sleepiness before nCPAP application. Long-term compliance with nCPAP may be mostly predicted from the usage pattern within the first three months of use.

• The Korean Journal of Pesticide Science
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• v.8 no.4
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• pp.338-346
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• 2004

The methanol extract of Chloranthus japonicus roots effectively controlled the development of rice blast(Magnaporthe grisea), rice sheath blight(Corticium sasaki), tomato pay mold(Btrytis cinerea), tomato late blight(Phytophthora infestans), and wheat leaf rust(Puccinia recondita). From the methanol extract of C. japonicus roots, three antifungal substances were isolated. Their chemical structures were determined to be shizukanols B, C, and D mainly by mass and NMR spectral data. Among the three substances, shizukanol C showed the strongest inhibitory activity against mycelial growth of the plant pathogenic fungi tested; it completely inhibited mycelial growth of M. grisea. Colletotrichum gloeosporioides, and C. acutatum at concentrations of more than $12.5{\mu}g$/ and P. infestans at concentrations of more than $3.13{\mu}g/m\ell$. They also controlled effectively the development of rice blast and wheat leaf rust. On the other hand, they caused phytotoxic symptoms on barley leaves and inhibited the growth of duckweed (Lemna paucicostata) with $EC_{50}$ values of $30.0{\mu}g/m\ell$ for shizukanol B, $49.9{\mu}g/m\ell$ for shizukanol C, and $154{\mu}g/m\ell$ for shizukanol D. In addition, shizukanol C showed an insecticidal activity against brown planthopper (Nilaparavata lugens), peen peach aphid (Myzus persicae), diamond-back moth (Plutella xylostella), and tobacco cutworm (Spodoptera litura) of the 5 arthropod pests tested with mortality values of more than 60% at a concentration of $1,000{\mu}g/m\ell$.

• Korean Journal of Organic Agriculture
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• v.18 no.1
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• pp.93-104
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• 2010

This study was conducted screening of the population densities of fungal pathogens and insect and observed the disease symptoms on the organic sweet corn seed producing field from 2008 to 2009. The dissemination spores, Alternaria sp., Cladosporium sp., Helminthosporium sp., Pyricularia sp., Collectotrichum sp., and Bipolaris sp., were detected and the three fungal spores from the front were observed for whole growth stage. Seed and seedling diseases were shown as the rotted seed and damping-off seedling caused by Penicillium sp. and Rhizoctonia sp.. The larva of Black cutworm cut down the root crown of seedlings. The damaged plants were ranged from 14% to 16%. On the Oriental corn borer, the population densities and the percentage of damaged plants were showed a low difference between two sweet corn varieties. The population densities of Oriental corn borer were scored as from 3.5 to 20.5 in 2008 and from 0.5 to 6 in 2009. Also the percentage of damaged plants were significantly increased until harvesting stage and was recorded from 7.5% to 21% in 2008 and from 1% to 46% in 2009. On the Corn leaf blight, the percentage of diseased plant were scored from 7% to 34% in 2008. The first occurrence of date was after June 18, and the percentage of diseased plant was continuously increased after August 21 and the values of diseased plants was ranged from 56% to 69% in 2009. On common smut, the percentage of diseased plant was recorded from 5% to 15% in 2008, and the first occurrence date were delayed as 17 days (July 17) and were showed less than 8% of diseased plants in 2009. Corn Southern Leaf spot was scored as average 11% at early stage and showed high score as 62% at September 19 in 2008. In 2009, the first occurrence date were advanced about 20 days (after June 8), and continuously increased up to 86% in 2009.