The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Screening of Antagonistic Bacteria Having Antifungal Activity against Brown Spot and Sheath Rot of Rice

벼 깨씨무늬병 및 잎집썩음병에 항진균 활성을 갖는 길항 미생물의 탐색

  • Ryu, Myeong Seon (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Yang, Hee-Jong (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Jeong, Su-Ji (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Seo, Ji-Won (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Jeong, Do-Youn (Microbial Institute for Fermentation Industry (MIFI))
  • 류명선 ((재)발효미생물산업진흥원) ;
  • 양희종 ((재)발효미생물산업진흥원) ;
  • 정수지 ((재)발효미생물산업진흥원) ;
  • 서지원 ((재)발효미생물산업진흥원) ;
  • 정도연 ((재)발효미생물산업진흥원)
  • Received : 2019.05.31
  • Accepted : 2019.07.11
  • Published : 2019.09.30
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Abstract

Brown spot and sheath rot of rice are caused by fungal pathogens such as Curvularia lunata, Cochliobolus miyabeanus, and Sarocladium oryzae, and cause losses such as reduced rice yield and quality, which is an enormous problem with serious long-term effects. To search biological control agents of phytopathogenic fungi, five kinds of useful Bacillus-like isolates which are excellent in extracellular enzyme activity and produce siderophore were selected from paddy soil of Sunchang in Korea. The selected isolates were tested for excellent antifungal activity against three of the phytopathogenic fungi that frequently occur in rice, and JSRB 177 strain had the most excellent antifungal activity. Based on the experimental results, JSRB 177 is finally selected as a candidate for biological control and identified to Bacillus subtilis through 16S rRNA sequence analysis. In addition, physiological characteristics of JSRB 177 confirmed by analysis of carbohydrate fermentation patterns and enzyme production ability. Based on the above results, JSRB 177 is expected to be used as a biological control agent for the rice pathogenic fungi. In the future, further studies related to industrialization such as port test and establishment of mass production process are needed.

벼의 깨씨무늬병과 잎집썩음병의 원인균인 Cochliobolus miyabeanus와 Sarocladium oryzae에 의해 발병되며, 깨씨무늬병이 이삭에 발생할 경우Curvularia lunata에 의해 이삭마름병이 추가로 발병하게 되고 한국인의 주식인 쌀의 수확량 감소 및 쌀의 품질 저하와 같은 손실을 초래하여 세계적으로 벼 재배 국가에서는 큰 문제로 장기적으로는 심각한 문제를 초래한다. 따라서, 이러한 식물 병원성 곰팡이의 생물학적 방제를 위해 순창군 논 토양에서 세포외 효소 활성이 우수하고 siderophore를 생산하는 유용미생물 5종을 선별하였다. 5종의 선별 미생물은 벼의 식물 병원성 곰팡이 3종에 대하여 우수한 항진균 활성을 갖고 있었으며, 특히 JSRB 177균주는 가장 우수한 활성을 지녀 최종 균주로 선별되었다. 최종 선별된 JSRB 177균주는 16S rRNA 염기서열 분석을 통하여 Bacillus subtilis로 동정되었으며, 최종적으로 JSRB 177의 당 이용성 및 효소 생산에 대한 분석을 통하여 생리학적 특성을 확인하였다. 향후 포트 시험 및 생산 공정 확립 등 산업화에 연관된 추가 연구가 필요하지만 앞선 결과를 토대로 JSRB 177 균주는 벼 병원성 곰팡이에 대한 생물학적 방제를 위한 소재로 높은 활용이 기대된다.

Keywords

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