Urea and ammonium sulfate without and with sulfur group, respectively, were compared with respect to their nutritive effects on the rice plants, Suweon 264 and Jinheung, under the nursery trial on the protected upland and lowland seedbed, the pot trials with their various combination ratios and with added sodium sulfate to urea and the trials on the paddy fields which have undergone urea and ammonium sulfate application for many years. The rice seedlings fertilized with ammonium sulfate surpassed those with urea in growth performance and nutrients absorption of the rice seedlings at nursery period. Such stimulating effect of ammonium sulfate on the growth and nutrients uptake was more remarkable under the upland nursery than under the lowland one. The pot trial with the various combination ratios of urea and ammonium sulfate revealed that the chlorophyll content in leaf blade increased with the increment of sulfate ratio in the combination and the sole application of urea caused the chlorosis of leaf which was more conspicuous in Suweon 264 than in Jinheung. Fertilized with the same active ingradient amount of nitrogen, the rice plants supplied with ammonium sulfate surpassed those with urea in the chlorophyll content consistently under the nursery, the pot and the paddy field trials. The photosynthesis of flag leaf at heading stage increased with the combination rate of ammonium sulfate in the pot trial. The sulfur applicated as supplementary element of nitrogen in the nursery, the pot and the field trials were observed to be in positive relationship to the nitrogen and potassium content, but to be in negative correlation to the calcium content. The sulfur content in the rice plants was higher at early growth stage and decreased with the advance in growth stage. The nitrogen content also showed a similiar tendency to the sulfur content, and the N/S ratio was higher at early growth stage than at later one. The N/S ratio was negatively correlated with the chlorophyll content. In the field experiment, ammonium sulfate surpassed urea in the number of productive tiller, dry matter production and unhulled rice yields, but much stimulating effect of ammonium sulfate on the grain production was shown to be less effective than that on the straw production. The nitrogen and major nutrients content in the rice straw at harvest were higher in the paddy field with long-term ammonium sulfate application than in that with long-term urea application, suggesting that the former might have greater potentiality in nutrients supply than the latter.
To use barley as a green manure crop, this study has been conducted in Suwon, Gyeonggi-do to establish the barley green manure (BGM)-rice cropping system with emphasis on soil incorporation date and BGM density. The nitrogen (N) contents of rye and barley, grass green manure crops, were 1.4~2.4% at early growth stage and decreased rapidly to 0.6~1.0% at late growth stage. The biomass of barley was 449 kg/l0a at heading stage (HS) and increased to 421 kg/10a at 10 days after heading stage (DAH), 473 kg/10a at 20 DAH. C/N ratio of BGM was the lowest 26.3 at HS. The N contents of BGM was in the range of 0.9~1.5%, the highest at HS and gradually decreased, and the output of N were 4.3-6.3 kg/10a. The total amount of nitrogen, phosphorous and potassium of BGM showed the highest level at 10 DAH. Culm length of rice was relatively longer as the BGM application time was delayed. The application of BGM into soil increased plant height of rice by 7.2~7.7 em as compared to the plants treated with commercial fertilizer at recommended rate. but panicle length of rice showed a similar tendency in both the soil-applied of BGM and commercial fertilizer. N contents of unhulled rice was the highest at HS of BGM and followed by 10 DAH of BGM and 20 DAH of BGM. This trend could also be seen in rice straw. The yield of rice in the soil-applied of BGM was 10~15% lower than in the soil-applied of commercial fertilizer. Based on this study, application of BGM made it possible to save 30~50% of application amount of nitrogen fertilizer for following crops.
The phosphorus(P) adsorption capacities of various filter media were investigated in relation to the size and types of fitter media to screen the optimum condition. The objective of this study was to evaluate the constructed wetland longevity by improving P adsorption capacity. The maximum P adsorption capacities of filter media A($4{\sim}10\;mm$), B($2{\sim}4\;mm$) and C($0.1{\sim}2\;mm$) were 8, 10 and 22 mg/kg, respectively, showing those increased as the filter media size decreased. Among the experimental media, the optimum filter media size was $0.1{\sim}2\;mm$. When the filter Medium was supplemented with organic materials which were piled up and decayed in the constructed wetland, the P adsorption capacity was significantly enhanced Under the conditions of optimum fitter media size, the respective Maximum P adsorption capacities of filter media C when supplemented with Ca, Mg, Al and Fe were higher than that of filter media C. However the addition of Ca, Mg, Al and Fe to constructed wetland were not recommended because of the possibility of their secondary pollution. The maximum P adsorption capacity of filter media C was 22 mg/kg, but this was increased to 36 mg/kg when filter media C was supplemented with 2% oyster shell.
BACKGROUND:Cultivation of alternative crops in paddy fields is necessary because of the decrease in rice consumption and the increase in excess stock of rice. The study was conducted to investigate the effects of alternative crops cultivation in paddy fields on soil physico-chemical characteristics and crop yield. METHODS AND RESULTS: Soybean (Glycine max), red-clover (Trifolium pratense), and water convolvulus (Ipomoea aquatica) were selected for alternative crops in the first and/or second year and rice was planted in the third year. When alternative crops were cultivated in the previous year, soil bulk density, soil hardness, and water content were lower than those for rice cultivation. Water-depth decreasing rate and aggregate content were greater for the upland-upland-paddy cropping system than upland-paddy-paddy cropping system. Cultivation of red-clover and water convolvulus for two years resulted in the high soil organic matter content. In the third year, available phosphate, exchangeable potassium, and soil cation exchange capacity were relatively high when soybean was cultivated in the previous year. In the first year, water convolvulus cultivation showed greater productivity than red-clover cultivation while the opposite pattern was found in the second year. Rice yield in the third year was greater for soybean or red-clover as a previous crop than for water convolvulus as a previous crop. CONCLUSION: The results suggest that cultivation of alternative crops in paddy fields can improve soil physical properties including bulk density, hardness, water content, and aggregate content as well as rice productivity.
The compost leachate was dark-colored solution that leaches out of the bottom of the compost pile. The compost leachate was rich in nutrients and can potentially used in plant culture. In the organic production, commercial liquid fertilizer was used to insure the availability of nutrients during the formation of the yield. The cost of supplemental liquid fertilizer could be reduced by developing a fertilizer based on animal fertilizer. This experiment was conducted to investigate the effect of different combination of compost leachate and conventional inorganic solution in hydroponic culture for lettuce growth. Six different treatments were applied. The compost leachate(CL) and nutrient solution(NS) were mixed by six different mixing ratios of 0:100, 20:80, 40:60, 60:40%, 80:20 and 100%:0% based on nitrogen content. The chemical nutrient solution was the solution of National Horticulture Research Station for the growth of leaf lettuce. The concentration of nutrient solution was adjusted 1.5 mS/cm in EC. The compost leachate was low in phosphorus(P), calcium(Ca), magnesium(Mg), but rich in potassium(K). The plant height of lettuce treated with CL 20+NS 80% was similar with 100% NS of control plot. Plant height was highest in the plot of CL 20+NS 80%. The treatment of 100% compost leachate was lowest in the growth characteristics of leaf lettuce. Number of leaf was very low in 100% compost leachate compared with plot of chemical nutrient solution. In the beginning of growth stage, SPAD value was reduced in plot treated with CL100%, but CL20+NS80% plot was higher compared to 100% compost leachate. SPAD value of leaf lettuce leaves was decreased as the amount of CL was increased. The dry weight of lettuce were 107.4, 104.2g in plot of NS 100% and CL 20%+NS80%, respectively. The leaf number and plant weight were decreased at high application plots of compost leachate. The leaf lettuce showed lowest in the plot treated with 100% compost leachate, and the growth of lettuce severely decreased after application of 100% CL treatment. The results showed that compost leachate can be use as liquid fertilizer for the organic hydroponic production. The mixture solution of 20% of compost leachate and 80% of nutrient solution could be used as a nutrition solution in hydroponic culture of leaf lettuce. Our studies have shown that is possible to produce using compost leachate, although growth is slower than when using a conventional inorganic hydroponic solution.
Journal of The Korean Society of Grassland and Forage Science
/
v.6
no.3
/
pp.131-137
/
1986
Korean lawn, Zoysia japonica STEUD, is the type of a perennial warm-season lawn grass that has a naturally green period during 5 months from May to September. This study was carried out to detect the effects of N-P-K fertilization, regrowth capacity after mowing and chlorophyll contents on prolongation of the green period in Zoysia japonica grasslands. The results obtained are summarized as follows: 1. The effects of nitrogen fertilizer application on Zoysia japonica grasslands were affected on regrowth capacity after mowing, green color of leaf canopies and chlorophyll contents, respectively. 2. A the experimental plots which applied 45g/$m^2$/10 weeks of $N_3$ fertilizer, there were high significant effects on prolongation of the green period and the plots of phosphorus and potassium had additive effects. 3. Prolongation of the green period to the last ten days of October was obviously possible by slow degrees in proportion to increment of N-P-K fertilization. 4. However, it is interpreted that more prolongation of the green period is impossible with N-P-K fertilization and irrigation treatments, unless the intermediate turfgrasses between $C_3$ and $C_4$ plants must be found and bred.
BACKGROUND: This experiment was carried out to evaluate the effect of potassium fertigation on the growth and yield of fourteen years-old 'Campbell Early' grapevine (Vitis labrusca L.) on a sandy loam soil. METHODS AND RESULTS: Potassium application (125 kg/ha/yr) was top-dressed as 30% of annual potassium application in all treatments and the remainders were drip-irrigated with 0 (0% of total K, K0 level of the remainder), 25 (17.5% of total K, K1/4 level of the remainder), 50 (35% of total K, K1/2 level), and 100mg/L (70% of total K, K1 level) in the intervals of 2 times a week for 12 weeks and the effect of K drip fertigation was compared to control treatment in which the K remainder was applied with surface application as additional fertilizer. The growth of stem diameter, leaf number and shoot length were highest in K1/2 (50 mg/L K), but K of mineral contents was lowest in K0. Yield of grapevine was no difference in 2008, but significantly higher K1/2 than other treatments in 2009. However, fruit quality (color degree, brix, acid contents) was no difference among the treatment. CONCLUSION(s): From the results, It is expected that K1/2 levels of fertigation based on soil testing could be more efficient to get optimum yield and save potassium fertilizer than control (surface application) treatments when grapevine was drip-irrigated at open field condition.
Journal of The Korean Society of Grassland and Forage Science
/
v.13
no.1
/
pp.43-48
/
1993
This experiment was carried out to determine the effects of application time of cattle slurry on grazing pasture on the grass growth, dry matter yield, nutritive value, animal preference, and efficiency of pasture utilization. Four different application times of slurry(right now after grazing, 5th day, 10th day, and 15th day after grazing), and a no-slurry(control) plot were designed, 1991∼1992. Annual application amount of slurry was 40 ㎥/ha, and slurry was applicated in spring(2 times), summer and autumn. Total NPK fertilizer applicated were same as 280, 200 and 240 kg/ha in all treatment, respectively. The pasture was grazed with growing beef cattle(initial body wt.; ca. 250 kg) at every 30∼35 cm of plant height. The grass height, dry matter yield, crude protein yield, and other nutritive value were not different among treatments. However, the animal preference and efficiency of pasture utilization showed significant difference by application time of slurry. The highest grazing preference (1: the worst∼9: the best). and utilization efficiency (0∼100%) were observed by application time of right now after grazing (7.8, 76%), which was same to control(7.8, 77%), and followed by 5th day after grazing(7.3, 74%) and l0th day after grazing(6.9, 71%). But the preference and pasture utilization were greatly decreased at application time of cattle slurry of 15th day alter grazing(5.5, 60%). From the above results, the optimum application time of cattle slurry on grazing pasture to enhance animal preference, and efficiency of pasture utilization was within 5 days after grazing (less than 13.6 cm of plant height), and within 10 days after grazing (less than 18.5 cm of plant height) at least.
This study was carried out to estimate the effect of NPK fertilization on tree seedling growth and chemical properties of soil. Seedlings of Betula platyphylla var. japonica and Fraxinus rhynchophylla were planted in May 1998 in the nursery at Gariwang mountain, Kangwondo, and seven NPK fertilizer plots(4, 12, 28, 60, 124, 252, and $508g/m^2$) and one non-fertilized plot were used to meet the study objectives. Seedlings of Pinus koraiensis and Acer mono were planted in April 1998 in plastic pots of nursery at Chungnam National University, and seven NPK fertilizer plots(0.5, 1.5, 3.5, 7.5, 15.5, 33.5 and 63.5g) and one non-fertilized plot were used to meet the study objectives. To estimate the relative growth ratios of the seedlings, height and root collar diameter of the seedlings were measured after planting and also in October 1998. Soil samples were collected in October 1998 from the nursery of Gariwang mountain and the plastic pot of the nursery of Chungnam National University. The relative growth ratios of height and root collar diameter in Betula platyphylla var. japonica, Fraxinus rhynchophylla and Acer mono were greater in all fertilized plot than non-fertilized plot, but there was no difference in relative growth ratio of height and root collar diameter of Pinus koraiensis between the fertilized and non-fertilized plots. NPK fertilization increased soil P and K, decreased exchangeable Ca and Mg, and base saturation, and pH. Fertilization resulted in no difference in total soil N between the fertilized and non-fertilized plots of nursery of Gariwang mountain, but increased in total soil N in plastic pots. Fertilization increased the growth of hardwood species and had changed soil chemical properties of this fertilizer trials.
It were reported that antifungal mechanism of Enterobacter cloacae is a volatile ammonia that produced by the strain in soil, and the production of ammonia is related to the bacterial urease activity. A powerful bacterium SH14 against soil-borne pathogen Fusarium solani, which cause root rot of many important crops, was selected from a ginseng pathogen suppressive soil. The strain SH14 was identified as Bacillus subtilis by cultural, biochemical, morphological method, and $API^{circledR}$ test. From several in vitro tests, the antifungal substance that is produced from B. subtilis SH14 was revealed as heat-stable and low-molecular weight antibiotic substance. In order to construct the multifunctional biocontrol agent, the urease gene of Bacillus pasteurii which can produce pathogenes-suppressive ammonia transferred into antifungal bacterium. First, a partial BamH I digestion fragment of plasmid pBU11 containing the alkalophilic B. pasteurii l1859 urease gene was inserted into the BamH I site of pEB203 and expressed in Escherichia coli JM109. The recombinant plasmid was designated as pGU366. The plasmid pGU366 containing urease gene was introduced into the B. subtilis SH14 with PEG-induced protoplast transformation (PIP) method. The urease gene was very stably expressed in the transformant of B. subtilis SH14. Also, the optimal conditions for transformation were established and the highest transformation frequency was obtained by treatment of lysozyme for 90 min, and then addition of 1.5 ${mu}g$/ml DNA and 40% PEG4000. From the in vitro antifungal test against F. solani, antifungal activity of B. subtilis SH14(pGu366) containing urease gene was much higher than that of the host strain. Genetical development of B. subtilis SH14 by transfer of urease gene can be responsible for enhanced biocontrol efficacy with its antibiotic action.
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