• Research in Plant Disease
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• v.6 no.1
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• pp.15-18
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• 2000

A new bacterial disease was found on leaves of Kalanchoe blossfeldiana plant grown under vinyl-house condition in winter of 1998 in Taejeon. the first symptoms of the disease are the appearance of the water-soaked and light brown spots. Later they become soft rot with brown color. Causal bacteria were isolated from diseased tissues and the same symptoms as the natural infection were developed on Kalanchoe blossfeldiana leaves by needle-prick inoculation. The causal bacterium was identified Erwinia hervicola by its bacteriological characteristics. This is the first reported of this bacterium to occur on kalanchoe blossfeldiana plant in Korea. Therefore, we proposed to name the diseases as \"bacterial soft rot of Kalanchoe blossfeldiana\" by E. herbiocla.

• Research in Plant Disease
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• v.9 no.3
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• pp.145-148
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• 2003

In April of 2002, the gray mold disease occurred severely on kalanchoe (Kalanch oe blossfeldiana) grown in a nursery in Daesan-myon, Changwon City, Korea. The lesions were started with water-soaked spot and the leaves discolored from the tip and became gray or dark and gradully expanded to whole leaf. The conidia and mycelia of pathogen appearedd on flower, flower stalk and stem. Conidia were gray, 1-celled, mostly ellipsoid or ovoid in shape and were 8~17 ${\times}$5~12${\mu}m$ in size. Conidiophores were 13~34${\mu}m$ in size. The sclerotia were formed abundantly on potato-dextrose agar. The optimum temperature for sclerotial formation was $20^{\circ}C$. Pathogenicity of the causal organism was proved on Kalanchoe blossfeldiana according to Koch,s postulate. The causal organism was identified as Botrytis cinerea based on mycological characteristics. This is the first report on gray mold of Kalanchoe(Kalanchoe blossfeldiana) caused by Botrytis cinerea in Korea.

• Journal of Bio-Environment Control
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• v.11 no.3
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• pp.108-114
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• 2002

Cellular glass foam (CGE), the reprocessed glass, has a possibility as a component of vegetative propagation media of floricultural crops due to the its excellent air and water permeability, similar to that of perlite. An experiment was conducted to evaluate the rooting and growth thereafter of Kalanchoe blossfeldiana ‘Gold Strike’in media containing various volume ratios of granular rockwool, peat-moss, CGF and perlite. The particle size of CGF and perlite was 2.0~4.0mm and 1.2~4.0mm, respectively. Cuttings were rooted in a fog tunnel with a mean temperature of 18.2$^{\circ}C$ and RH of 66.7％ under a long day regime (14 h per day light period). Height, length of the longest root, stem diameter, no. of leaves, leaf area, percentage of rooted cuttings, shoot and root fresh weights, shoot and root dry weights, total chlorophyll concentration and physicochemical properties were measured. Cuttings rooted 100% in all treatments. Physicochemical properties in CGF and perlite-containing media showed little differences. The growth of rooted plants in the CGF-containing media was similar or rather superior to that in perlite-containing media. Consequently, CGF has a possibility as a vegetative propagation medium of Kalanchoe. To make wider commercial use of CGF, more demonstrative experiments and analyses are necessary.

• Research in Plant Disease
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• v.13 no.2
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• pp.122-125
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• 2007

Leaf spot disease of Kalanchoe (Kalanchoe blossfeldiana) occurred at the farmer's vinly house in Gangseo-gu, Busan Metropolitan City, Korea, 2006. The diseased plants with typical symptom were collected and the casual agent were isolated. Its mycological characteristics and pathogenicity were examined. The results were as follows. The typical symptoms of the disease appeared as small brownish or dark brown spot on both sides of the leaves. The spots tended to develop from lower leaves. The spots gradually enlarged into conspicuous necrotic lesions 1-5 mm in diameter. Colonies of the causal fungus formed on potato dextrose agar were velvety, gray or grayish brown in color, Conidia were cylindrical or obclavate to oblong in shape, brown in color, $24{\sim}65\;{\times}\;12{\sim}23\;{\mu}m$ in size and had 1-4 transverse septa, The optimum temperature for growth of the fungus was about $25-30^{\circ}C$. Conidiophores were brown in color, $32{\sim}135\;{\times}\;4{\sim}8\;{\mu}m$ in size and had 1-7 transverse septa. The fungus was identified as Stemphylium lycopersici (Enjoji) Yamamoto based on its symptom and mycological characteristics. This is the first report of leaf spot of Kalanchoe caused by S. lycopersici in Korea.

• FLOWER RESEARCH JOURNAL
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• v.18 no.1
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• pp.50-56
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• 2010

This experiment was carried out to examine the effect of a silicate fertilizer on the growth and development of potted plants. Cutting of Kalanchoe blossfeldiana 'Kaluna' and 'Taos', and Dianthus caryophyllus L. 'Kazan' and 'Tula' were grown in 50 and 128-cell plug trays, respectively. Rooted cuttings transplanted to the mixture of a commercial medium and perlite (5:1, v/v) supplemented with a silicate fertilizer at 0, 40, 80, 120 or $160g{\cdot}L^{-1}$ medium was evaluated. A silicate fertilizer supplementation at $40g{\cdot}L^{-1}$ medium resulted in the greatest plant height, leaf thickness, and root fresh and dry weights in both kalanchoe and carnation. However, plant height was suppressed in the treatment of a silicate fertilizer supplementation at higher concentrations in both kalanchoe and carnation. According to the scanning electron microscope images of transversal sections of tissues of roots and leaves in kalanchoe and carnation, the treatment of a silicate fertilizer supplementation at $40g{\cdot}L^{-1}$ medium resulted in plants with more compact tissue than the control.

• Korean Journal of Plant Resources
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• v.10 no.1
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• pp.86-93
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• 1997

In order to induce somatic embryogenesis from the stem explants and anther of Kalanchoe daigremontiana, the explants were cultured on MS medium supplemented with auxin (2,4-D, IAA, NAA) and/or cytokinin (BAP) for 8 weeks. Callus from explants was induced most efficiently on MS medium containing. 2.0mg/L NAA and 0.2mg/L BAP. Somatic embryogenesis in stem callus was formed by transfering embryogenic callus from induction media containing growth regulators to medium without growth regulators and then to the medium containing auxin and cytokinin (0.1 mg/L IAA and 1.0mg/L BAP). Callus formation occurred actively in the anthers at early uninucleate stage, and by low temperature pretreatment at $4^{\circ}C$ for 3days. Somatic embryogenesis from the anther callus was induced on MS medium containing 1.0mg/L NAA and 1.0mg/L BAP, 2.0mg/L NAA and 0.2mg/L BAP. The tetraploid of 5.4% was obtained among plants regenerated from anthers.

• The Journal of the Convergence on Culture Technology
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• v.9 no.3
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• pp.601-606
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• 2023

The edible sweetener aspartame is formed from the synthesis of asparagine, one of the amino acids, and phenylalanine. It is a protein ingredient that produces sweetness 200 times higher than sucrose, and is used as a diet product because it is a substance that can replace sugar. Although the effects on animals and humans have been well studied, no studies have been conducted on plant metabolism. In this study, the effect of aspartame metabolism was tested using germinated onion, bean sprouts, and Kalanchoe. The three types of plants with germinated roots showed rapid growth in the early stage of treatment with 1mM aspartame, and showed a nutrient supply effect due to the effect of amino acids, which are constituents. However, when treated for 4 weeks, symptoms similar to lateral roots appear in the form of lignification in onions, the effect of quickly withering is seen in bean sprouts, and senescence of roots appears in 12-week-old Kalanchoe. All of them show accelerated aging after the growth phase. This shows that, among the two amino acids, asparagine is related to auxin generation that induces rapid growth, and it is thought to be the result of lignification due to the effect of phenylalanine.