• Journal of the korean academy of Pediatric Dentistry
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• v.45 no.1
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• pp.32-40
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• 2018

The aim of this study was to determine the efficacy of a 3 tone plaque disclosing gel in assessing the risk of caries related to the population of Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus spp. quantified using a quantitative real-time polymerase chain reaction (qRT-PCR). 15 healthy children of ages 9 - 12 years were randomly examined. The 3 tone plaque disclosing gel was applied on teeth surfaces, which changed the color to pink or red, blue or purple and light blue. Plaque was divided into 3 groups based on staining. Genomic DNA from each sample was subjected to a qRT-PCR assay for quantitative detection of target bacteria. The Kruskal-Wallis test was conducted for correlation between the color of plaque and the number of bacterial species. The levels of S. mutans, S. sobrinus, and Lactobacillus spp. were significantly different in the plaque samples of the 3 groups (p < 0.05). The proportion of S. sobrinus to S. mutans showed correlation to the color of plaque. The different color-dyed plaque was related to the number of acidogenic bacteria. The 3 tone plaque disclosing gel could be used as one of the indicators to assess the clinical risk of caries associated with the population of S. mutans, S. sobrinus, and Lactobacillus spp.

• Journal of the korean academy of Pediatric Dentistry
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• v.44 no.4
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• pp.419-426
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• 2017

Attachment of the orthodontic bracket conducted during the window opening procedure can result in failure due to various adverse conditions such as blood or saliva contamination, or other wet conditions. For the success of the bracket attachment, reduction of total operation time is crucial. The purpose of this literature is to evaluate the differences between the final resultant shear bond strength of the conventional method of using phosphoric acid and self-etching primer, and that of the operation time-reduced method in which the curing step is omitted subsequent to the primer application. A total of 40 human maxillary incisors were prepared. Group I(control group) and II were etched with 37% phosphoric acid and Group III and IV were conditioned with self-etching primer. Attachment of the group I and III was conducted by manufacturer's instructions. The operation time of Group II and IV was reduced by eliminating the curing step after the primer application. The resultant shear bond strength of each group was measured and an adhesive remnant index (ARI) was recorded. The mean shear bond strength of group I, II, III, and IV were 14.16 MPa, 8.33 MPa, 8.29 MPa, and 6.48 MPa respectively. Significant differences could only be found between the control group and the experimental groups (p < 0.05). The ARI indicated no significant difference among all groups.

• The korean journal of orthodontics
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• v.35 no.4 s.111
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• pp.302-311
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• 2005

This study was performed to compare the fatigue limit of stainless steel wires and Fiber-reinforced composites (FRC) under conditions of permitting physiologic tooth movement. and to evaluate the clinical value of FRCs which was used to reinforce the anchorage unit. The stainless steel wire groups were divided into round and rectangular wire groups. The FRC groups were divided into uni-directional and woven groups, with resin coating and without resin coating in the Proximal area After the number of cycles to failure of each of the 6 groups were measured within the $5{\times}10^5\;cycle$ fatigue limit simulating the orthodontic treatment period. the fatigue limit of each group was compared with each other The findings of this study were as follows. In stainless steel wires, the fatigue limit of rectangular wires were higher than that of round wires. But there was no statistically significant difference (p>0.05). In FRCs with resin coating and without resin coating in the interproximal area, the fatigue limit of uni-directional type was higher than that of the woven type (p<0.05). In uni-directional and woven type FRCs, the fatigue limit of FRC with resin coating in the interproximal area was higher thar that of FRC without resin coating (P<0.05) As the FRCs and stainless steel wires did not fracture until the $5{\times}10^5\;cycle$ fatigue limit which clinically is useful. it is sufficient to use FRC and stainless steel wire for reinforcing anchorage. When esthetics is important and the attachment of additional devices are necessary. it seems sufficient to use FRC as anchorage reinforcement.

• The korean journal of orthodontics
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• v.35 no.1 s.108
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• pp.43-50
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• 2005

The purpose of this study was to evaluate the effects of a sealant resin on enamel demineralization In orthodontic bracket bonding. The forty eight extracted sound bovine teeth were subdivided into four groups and treated with Phase II (Reliance, itasca. III) on the surface Group 1 was not treated. Group 2 was acid etched with 37% phosphoric acid for 30 seconds. Group 3 was applied with sealant after acid etching. Group 4 was applied with resin paste after acid etching and sealant application. Each group was demineralized in artificial caries solution. Demineralized enamel depth was measured by confocal laser scanning microscopy. The results were as follows: the mean demineralized enamel depth was $47.4{\mu}m$. (Group 1), $61.8{\mu}m$ (Group 2), $13.9{\mu}m$ (Group 3). $8.2{\mu}m$ (Group 4) the demineralized enamel depth was increased in Group 2 than in Group 1 (p<0.05); the demineralized enamel depth was reduced in Group 3 than in Group 1 and Group 2 with statistically significant differences (p<0.05): and demineralization in Group 4 was very little. The results of the present study indicate that sealant application is useful for reducing enamel demineralization in orthodontic bracket bonding.

• The korean journal of orthodontics
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• v.35 no.1 s.108
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• pp.51-59
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• 2005

The aim of this study was to compare the species and biotypes of mutans streptococci isolated from dental plaques sampled from the interfaces between the bracket aid tooth surface and smooth tooth surfaces In orthodontic patients. Dental plaque was collected from the interfaces between brackets aid teeth (test group), and from smooth tooth surfaces distant from brackets by more that 2mm (control group). The dental plaque collected by a sterilized curette was transferred into a vial of 1 X PBS. The sample in the vial was vigorously vortexed for 1 min and plated ou mitis-salivarius bacitracin (MSB) agar plate using cotton tips. The agar plates were incubated at $37^[\circ}C$ in a candle jar for 2 days, and again incubated for 1 more day at anambient temperature Individual colonies were cultured in TH broth at $37^[\circ}C\;CO_2$ incubator. The PCR-RFLP based on dextranase gene was performed for the identification of mutans streptococci at the species-level For biotyping of mutans streptococci, biochemical tests were performed There was no significant difference of the species of mutans streptococci isolated from both test and control groups However, the biotypes of the mutans streptococci isolated from test and control groups were different. These results may offer the basic data to verify the relationship between the mutans streptococci biotype and enamel decalcification or dental caries in orthodontic patients with fixed appliances.

• The korean journal of orthodontics
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• v.28 no.1 s.66
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• pp.51-59
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• 1998

The purpose of this study was to evaluate the effectiveness of gargling solution with 0.05% NaF and 10% Xylitol in orthodontic patients with fixed appliance. The sample consisted of 30 adolescent patients who were classified into an experimental group and a control group, 15 patients each. Experimental group was used experimental gargling solution and the control group was used with placebo solution. The change of S. mutans in saliva was observed by $Cariescreen^R$ SM kit at pre and post 2, 4, 6, 8 weeks. The results were as follows 1. There were significant reduction in the number of S. mutans in saliva between pre and post 2 weeks(p<0.01), 4 weeks(p<0.05), 6 weeks(p<0.001), and 8 weeks(p<0.001) in experimental group. And significant reduction also were observed in the number of S. mutans in saliva between post 2 weeks, 6 weeks(p<0.05), and 8 weeks(p<0.05), but no significant reduction were showed in control group. 2. There were significant correlation in the number of S. mutans between each measurement time(pre and post 2, 4, 6, 8 weeks) in control group. 3. There were no correlation between pre and post 2 weeks, but significant correlation were observed between 2, 4, 6, 8 weeks in experimental group.

• Journal of the korean academy of Pediatric Dentistry
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• v.38 no.2
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• pp.137-145
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• 2011

The purpose of this study was to obtain the data for dental caries experience and occlusal pattern to utilize these data for motivating oral health promotion and activating oral health care programme in preschool children of multicultural family. Caries prevalence on primary teeth was surveyed for 3-5 years old 190 children of multicultural families in Chonbuk province. The results were as follows : 1. Dental caries prevalence rate of primary teeth (dmf rate) was 61.7% at 3 years of age, 88.7% at 4 years of age, and 92.6% at 5 years of age (p>0.05). 2. Average score of dmft index was 2.22 at 3 years of age, 4.97 at 4 years of age, and 6.62 at 5 years of age (p>0.05). 3. Most of the caries-prevailed tooth was maxillary primary central incisor and showed 28.8% at 3 years of age, 56.7% at 5 years of age. 4. dmft index and dmfs index of children of Vietnamese, Cambodian, and Filipinos women were higher than those of Chinese and Japanese. 5. In sagittal primary molar relationship, flush terminal plane type was 82.6%, distal step type was 5.2% and mesial step type was 8.4%. This study is the first report for children of multicultural families in Korea. More attention for children of multicultural families will be needed in aspect of oral disease prevention and treatment.

• Journal of the korean academy of Pediatric Dentistry
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• v.37 no.4
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• pp.453-460
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• 2010

This study was one of the caries pattern studies. The purpose was to investigate the degree of intraoral symmetry of dental caries in the primary dentition. The dmfs data from children aged 36 to 71 months were analyzed. Pearson correlation coefficients between right teeth and left teeth were from 0.558 (upper canines) to 0.847 (upper central incisors) and 0.905 in total (P<0.01). Differences between right teeth and left teeth were mostly not significant (P>0.05). Pearson correlation coefficients between upper teeth and lower teeth were from 0.150 (right lateral incisors) to 0.506 (right first molars) and 0.680 in total (P<0.01). Differences between upper teeth and lower teeth were mostly significant (P<0.05). Percentages of bilateral caries experience cases per cases having caries experience were from 34.0% (upper canines) to 80.1% (upper central incisors), and from 53.9% (distal) to 84.9% (mesial) in upper central incisors, from 34.1% (mesial) to 45.0% (occlusal) in upper first molars, from 20.7% (distal) to 48.0% (occlusal) in upper second molars, from 34.4% (buccal) to 58.1% (occlusal) in lower first molars, and from 29.7% (distal) to 61.4% (occlusal) in lower second molars, respectively.

• Journal of the korean academy of Pediatric Dentistry
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• v.37 no.2
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• pp.186-192
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• 2010

Streptococcus mutans, one of the major causal agents of dental caries, is component of the dental plaque. It produces various organic acids such as lactic acid which is the end-product of glycolysis, and this leads to dental caries. A new system using species-specific monoclonal antibodies was developed to detect Streptococcus mutans in saliva. The system quickly detects salivary Streptococcus mutans in 30min and classifies the result into two levels. The purpose of this study was to investigate correlation between monoclonal antibody-based detecting system and selective medium-based detecting methods. Children's deft indices were also compared with Streptococcus mutans counts in MSB agar plate. Subjects consisted of 15 children in the age of 3 to 6 years. They were assigned to three groups : Group I(deft index = 3), Group II(deft index $\leqq$3), Group III(deft index $\geqq$4). The results are as follows : 1. The rate of children with positive response was 13.3% and with negative response was 86.7% in the result of Saliva-checkTM Mutans test kit. 2. There was a positive correlation between monoclonal antibody-based detecting system and selective medium-based detecting methods(p<0.05). 3. Streptococcus mutans counts in MSB agar plate were irrelevant to deft of children(p=0.34).

• Journal of the korean academy of Pediatric Dentistry
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• v.37 no.4
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• pp.445-452
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• 2010

Recently the effect of CPP-ACP on early caries remineralization and prevention has hashbeen extensively researched. However, there has been a lack of research on micro-shear bond strength of a sealant applied on a CPP-ACP treated surface. Therefore, the purpose of this study was to evaluate the effect of CPP-ACP on the micro-shear bond strength of a sealant bonded with 3 different bonding systems. From the sound human 3rd molars, the 150 specimens were prepared and randomly assigned to six groups. Group I: Immersion in artificial saliva for 2 weeks + 35% phosphoric acid Group II: Immersion in artificial saliva for 2 weeks + 35% phosphoric acid + dentin bonding agent Group III: Immersion in artificial saliva for 2 weeks + self-etching adhesive Group IV: CPP-ACP application for 2 weeks + 35% phosphoric acid Group V: CPP-ACP application for 2 weeks + 35% phosphoric acid + dentin bonding agent Group VI: CPP-ACP application for 2 weeks + self-etching adhesive Sealant was applied and the micro-shear bond strength was measured. From the result of this study, it can be assumed that the CPP-ACP pretreatment can weaken the bond strength of a sealant if the enamel surface is conditioned with self-etch adhesive.