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Identification of mutans streptococci isolated from dental plaque between the bracket and tooth surface in orthodontic patients  

Kim, Mi-Ae (Department of Orthodontics, College of Dentistry, Chosun University)
Yoo, So-Young (Department of Oral Biochemistry, College of Dentistry, Chosun University)
Kim, Hwa-Sook (Department of Oral Biochemistry, College of Dentistry, Chosun University)
Kook, Joong-Ki (Department of Oral Biochemistry, College of Dentistry, Chosun University)
Lim, Sung-Hoon (Department of Orthodontics, College of Dentistry, Chosun University)
Yoon, Young-Jooh (Department of Orthodontics, College of Dentistry, Chosun University)
Kim, Kwang-Won (Department of Orthodontics, College of Dentistry, Chosun University)
Publication Information
The korean journal of orthodontics / v.35, no.1, 2005 , pp. 51-59 More about this Journal
Abstract
The aim of this study was to compare the species and biotypes of mutans streptococci isolated from dental plaques sampled from the interfaces between the bracket aid tooth surface and smooth tooth surfaces In orthodontic patients. Dental plaque was collected from the interfaces between brackets aid teeth (test group), and from smooth tooth surfaces distant from brackets by more that 2mm (control group). The dental plaque collected by a sterilized curette was transferred into a vial of 1 X PBS. The sample in the vial was vigorously vortexed for 1 min and plated ou mitis-salivarius bacitracin (MSB) agar plate using cotton tips. The agar plates were incubated at $37^[\circ}C$ in a candle jar for 2 days, and again incubated for 1 more day at anambient temperature Individual colonies were cultured in TH broth at $37^[\circ}C\;CO_2$ incubator. The PCR-RFLP based on dextranase gene was performed for the identification of mutans streptococci at the species-level For biotyping of mutans streptococci, biochemical tests were performed There was no significant difference of the species of mutans streptococci isolated from both test and control groups However, the biotypes of the mutans streptococci isolated from test and control groups were different. These results may offer the basic data to verify the relationship between the mutans streptococci biotype and enamel decalcification or dental caries in orthodontic patients with fixed appliances.
Keywords
Mutans streptococci; Bracket; Biotype; Species;
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