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PCR-RFLP patterns of three kinds of Metagonimus in Korea (국내에 존재하는 세 종류 메타고니무스속 흡충의 RCR-RFLP반응양상)

  • 유재란;정진성
    • Parasites, Hosts and Diseases
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    • v.35 no.4
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    • pp.271-276
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    • 1997
  • We tried to compare the three kinds oi Metagonimur species. M. Wokognulci, Ifetafonimus Miyata type, and M. tnknhashii, which were Know to be distributed in Korea with polymerase chain reaction based-restriction fragment length polymorphism (PCR- RFLP) patterns. We amplified the internal transcribed spacer 1 (ITSI) site of ribosomal RNA and mitochondrial cytochrome c oxidase I (mCOI) gene. The restriction patterns of ITSI gene loth Rsc I, ALu I and Msp I showed multiple fragmented bands of different sizes between three kinds of Metcgonimus. In case of mc01 gene, Rsc I and Alu I enzymes produced differentially fragmented band patterns. According to the parsimony analysis of PCR-RFLP patterns, the estimated genetic divergence between M Wokognwai and Metasoninus Miyata type was 0.034880, between Metusoninus Miyata type and M. tckc- hushii was 0.028098, between M. wokogawai and M. tnkahashii was 0.018179. It is suggested that Metasonimus Miyata type may be separate species and evolutionize at the older time than the other two species.

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Effect of Clonorchis sinensis infection and dimethylnitrosamine administration on the induction of cholangiocarcinoma in Syrian golden hamsters (Dimethylnitrosamine을 투여한 햄스터에서 간홉충감염이 담관암 발생에 미치는 영향)

  • Lee, Jae-Hyeon;Im, Han-Jong;Park, Eung-Bok
    • Parasites, Hosts and Diseases
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    • v.31 no.1
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    • pp.21-30
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    • 1993
  • The study was carried out to observe the effects of Clonorchis sinensis Infection on induction of cholangiocarcinoma in Syrian golden hamsters to which 15 ppm dimethylnitrosamine (DMN) solution was administered for 8 weeks. The histopathological changes of the bile duct and liver cells were observed at the 11th week. In six of 8 hamsters (75%) which were treated with DMN and then infected with C. sinenis, the livers developed cholangiocarcinoma at 10 weeks after the infestation of C. sinenis. The features of cholangiocarcinoma lesions were adenomatous or papillary hyperplasia of the bile duct epithelia showing distinct anaplastic changes with mucinous cell metaplasia and necrotic area. In the hamsters which received either DMN or C. sinenis alone, the livers showed only hyperplastic changes of the bile duct epithelial cells. It was suggested that C. sinensis infection and DMN administration could be a synergism on the development of cholangiocarcinoma in Syrian golden hamsters.

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Foramniferal Characteristics in the Ganghwa Tidal Flat (강화 남부 갯벌의 유공충 특성)

  • Woo, Han Jun;Lee, Yeon Gyu
    • Journal of Wetlands Research
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    • v.8 no.3
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    • pp.51-65
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    • 2006
  • Surface sediments for sedimentary analyses were sampled at 199 stations in the study area in August 2003. The surface sediments consisted of six sedimentary facies. Generally, sandy mud sediments dominated in the southern tidal flat of Ganghwa Island and sand sediments dominated in channel and subtidal zones of the western part of Ganghwa Island. The area of sandy mud sediment extended to eastward tidal flat compared to sedimentary facies in August 1997. In 30 surface sediment samples from the Ganghwa tidal flat and subtidal zone, 61 species were recorded in total assemblages, including 34 species of living population. Ammonia beccarii and Elphidium etigoense in living population and Ammonia beccarii, Elphidium etigoense, Jadammina sp. and Textularia earlandi in total assemblage were widely distributed. Generally, relatively large numbers of species and high values of species diversity occurred in the area of western part of tidal flat. Cluster analysis of total assemblages discriminates four biofacies. Biofacies 1 indicated eastern part of the tidal flat and biofacies 4 indicated western part of the tidal flat. Biofacies 3 were transitional zone between biofacies 1 and 4.

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Development of Microclimate-based Smart farm Predictive Platform for Intelligent Agricultural Services (지능형 농업 서비스를 위한 미기상기반 스마트팜 예측 플랫폼 개발)

  • Moon, Aekyung;Lee, Eunryung;Kim, Seunghan
    • Journal of Korea Society of Industrial Information Systems
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    • v.26 no.1
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    • pp.21-29
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    • 2021
  • The emerging smart world based on IoT requires deployment of a large number of diverse sensors to generate data pertaining to different applications. Recent years have witnessed a plethora of IoT solutions beneficial to various application domains, IoT techniques also help boost agricultural productivity by increasing crop yields and reducing losses. This paper presents a predictive IoT smart farm platform for forcast services. We built an online agricultural forecasting service that collects microclimate data from weather stations in real-time. To demonstrate effectiveness of our proposed system, we designed a frost and pest forecasting modes on the microclimate data collected from weather stations, notifies the possibilities of frost, and sends pest forecast messages to farmers using push services so that they can protect crops against damages. It is expected to provide effectively that more precise climate forecasts thus could potentially precision agricultural services to reduce crop damages and unnecessary costs, such as the use of non-essential pesticides.

Observation of Items Fed by Noctiluca Scintillans around Dokdo in Spring (춘계 독도 주변해역 야광충의 먹이생물)

  • KANG, JUNG-HOON
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.25 no.4
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    • pp.160-172
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    • 2020
  • To understand on-site feeding traits of Noctiluca scintillans with unprecedented high abundances around Dokdo in spring, 2014, the composition and abundance of food items in the digestive vacuole of the species were analyzed. Abundances of N. scintillans ranged from 4,328~17,791 inds.m-3 around Dokdo during the study. The incidence of preyed items in the vacuole of N. scintillans ranged by averaged 32% (24~50%) in the surface waters around Dokdo. Diverse preyed items consisted of fecal pellet, protozoans, pollen, diatoms, dinoflagellates, copepods and ichtyoplankton, while fecal pellets were dominantly fed by N. scintillans (43%) and next by protozoans (19%), pollen (18%), diatoms (7%) and dinoflagellates (4%). Fecal pellet, protozoans and pollen were relatively preferred by N. scintillans compared to diatoms, dinoflagellates and ichtyoplankton based on the incidence in the food vacuoles. Present results indicated that high abundance of N. scintillans found around Dokdo was likely associated with non-phytoplankton items rather than previously well-known diatoms as prey during the study.

The First Report on the Acanthocephalan Infection of the Dybowskii's Brown Frogs (Rana dybowskii) Collected Inside and Outside the Commercial Frog Farms in Korea (국내 개구리 양식장 내·외에서 채집된 북방산개구리(Rana dybowskii)의 구두충 감염 최초보고)

  • Kim, Jong-Sun;Koo, Kyo-Soung;Park, Jae-Jin;Kwon, Sera;Choia, Woo-Jin;Cho, Han-Na;Park, Daesik
    • Korean Journal of Environment and Ecology
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    • v.30 no.4
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    • pp.694-704
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    • 2016
  • Various infectious and parasitic diseases are known to be the main factors that cause decline in the global amphibian population. In Korea, commercial frog farms have been running since 2005. However, until now, studies on diseases including studies on parasitic diseases that occur in farm frogs have not been conducted. In this study, we studied and compared the acanthocephalan infection rates and the number of parasites in the body cavity, stomach, and small and large intestines of the Dybowskii's brown frogs collected from inside and outside the frog farms in Inje, Goesan, Gongju and Boryeong. In addition, we classified the acanthocephalan parasites into genera by analyzing their nuclear 18S rRNA genes. On an average, 51.7% of the investigated frogs were infected by acanthocephalans, which belong to the Centrorhynchus genus. The infection rate of the frogs collected in the Inje farm was 15%, significantly lower than those from the Goesan, Gongju and Boryeong farms. The rate in Goesan was 55%, which is lower than Gongju (80%) and Boryeong (90%) although it is not statistically significant. No difference was found in the infection rate and in the number of parasites in male and female frogs and between the groups collected from inside and outside of the farms. The number of infected parasites negatively correlated with the body condition of the frogs. The most parasites were found in the stomach followed by the small and large intestines and the body cavity. This study is the first report on the Centrorhynchus acanthocephalan infection of amphibians in Korea and it suggests the necessity for acanthocephalan parasite management and for conducting further disease-related studies in commercial frog farms.

Use of Parasites for Stock Analysis of Salmonid Fishes (연어과 어류의 계군분석을 위한 기생충의 활용)

  • Kim, Jeong-Ho
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.12 no.2
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    • pp.112-120
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    • 2007
  • This paper reviews the use of parasites as 'biological tags' for studying stock analysis of salmonid fishes. Numerous definitions of stock concepts exist, but most of them essentially define a group of fish as having similar biological characteristics and being self-reproducing as stocks. It is important to manage fish stocks for human consumption and sustainable production and especially for salmonid fishes. Because these fry are considered as each country's property, it is necessary to identify and discriminate each fish stock in the open sea. Methods of separating fish stocks are very diverse. Artificial tags, parasites, otoliths scales and genetic characters have been used for stock analysis and each method has advantages and disadvantages. Of these parasites can be good biological tags because they are applied by nature at no cost. Parasites can be infected with susceptible host fishes when they enter into certain areas. Then if they move to the outside and are caught researchers can infer that the fish had been in the endemic area for a period of time during their life. Hence the host fish can be considered as naturally 'tagged' by parasites. However, if they do not pass the parasites-endemic. area, they will harbour no parasites. Therefore, researchers can discriminate each fish stocks and trace their migration routes with these biological tags. In this paper, several examples on the use of parasites as biological tags for studying salmonids, as well as other species, are listed. The advantages and limitations of parasites as biological tags are also discussed. Chum salmon (Oncorhynchus keta), the main salmonid species migrating to Korea, is distributed all around the North Pacific. Korean chum salmon are generally thought to move to the Sea of Okhotsk, the western North Pacific and the Bering Sea. However, there is no clear information on the distribution and migration pathways of Korean chum salmon, and no markers exist for separating them from others yet. Recent Korean chum salmon stock analysis including parasites information are mentioned.

Design and Implementation of Produce Farming Field-Oriented Smart Pest Information Retrieval System based on Mobile for u-Farm (u-Farm을 위한 모바일 기반의 농작물 재배 현장 중심형 스마트 병해충 정보검색 시스템 설계 및 구현)

  • Kang, Ju-Hee;Jung, Se-Hoon;Nor, Sun-Sik;So, Won-Ho;Sim, Chun-Bo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.10 no.10
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    • pp.1145-1156
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    • 2015
  • There is a shortage of mobile application systems readily applicable to the field of crop cultivation in relation to diseases and insect pests directly connected to the quality of crops. Most of system have been devoted to diseases and insect pests that would offer full predictions and basic information about diseases and insect pests currently. But for lack of the instant diagnostic functions seriously and the field of crop cultivation, we design and implement a crop cultivation field-oriented smart diseases and insect pests information retrieval system based on mobile for u-Farm. The proposed system had such advantages as providing information about diseases and insect pests in the field of crop cultivation and allowing the users to check the information with their smart-phones real-time based on the Lucene, a search library useful for the specialized analysis of images, and JSON data structure. And it was designed based on object-oriented modeling to increase its expandability and reusability. It was capable of search based on such image characteristic information as colors as well as the meta-information of crops and meta-information-based texts. The system was full of great merits including the implementation of u-Farm, the real-time check, and management of crop yields and diseases and insect pests by both the farmers and cultivation field managers.

Genetic variance of Tuchomonns uaginclis isolates by Southern hybridization (Southern hybridization에 의한 질편모충의 유전학적 다양성)

  • 류재숙;민득영
    • Parasites, Hosts and Diseases
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    • v.36 no.3
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    • pp.207-212
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    • 1998
  • In the present study, genomic DNAs were purified from Korean isolates (KT8, KT6, KT-Kim and KT-Lee) and foreign strains (CDC85, IR78 and NYH 286) of 1Trichomonas voslnalis, and hybridized with a probe based on the repetitive sequence cloned from T. uqfinolis to observe the genetic differences. By Southern hybridization, all isolates of T. uoSinoLis except the NYH386 strain had 11 bands. Therefore all isolates examined were distinguishable into 3 groups according to their banding patterns; i) KT8, KT6 and KT-Kim isolates had 11 identical bands such as 1 kb, 1.2 kb, 1.6 kb, 1.9 kb, 2.3 kb. 27 kb, 3.2 kb, 2.4 kb, 3.8 kb, 4.9 kb and 6.0 kb, ii) The metronidazole-resistant IR78 strain had the some bands as KT-Lee isolate at bands of 1 kb, 1.2 kb, 1.6 kb. 1.8 kb, 2.1 kb, 2.5 kb, 2.7 kb, 2.9 kb, 3.4 kb, 5.0 kb and 6.0 kb, Bands of CDC85, metronidazole-resistant strain, were similar to those of IR78 and KT-Lee, except that 3.2 kb replaced 2.9 kb. iii) NYH286 particularly had 12 bands and bun patterns were similar to IR78 with a few exceptions as follows; i) 6.2 kb in place of 6.0 kb, ii) 2.0 kb and 2.2 kb instead of 2.1 kb. Through the results obtained, genetic variance of T. uoginnlis isolates was demonstrated by Southern hybridization.

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농업기술 - 장마철 약용작물 병해충 방제기술

  • Park, Cheol-Gyu
    • 농업기술회보
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    • v.51 no.4
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    • pp.28-29
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    • 2014
  • 장마철 집중호우로 인해 배수불량 지대에서 작물이 장기간 침수되면 뿌리활력 저하로 인한 습해와 토양전염성병, 탄저병, 점무늬병 등 지상부 병해가 나타나다. 이번달에는 고품질 약용작물을 생산하기 위해 장마철 전 후 약용작물의 병해충 방제방법에 대해 알아본다.

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