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http://dx.doi.org/10.13047/KJEE.2016.30.4.694

The First Report on the Acanthocephalan Infection of the Dybowskii's Brown Frogs (Rana dybowskii) Collected Inside and Outside the Commercial Frog Farms in Korea  

Kim, Jong-Sun (Dept. of Biology, Kangwon National Univ.)
Koo, Kyo-Soung (Dept. of Biology, Kangwon National Univ.)
Park, Jae-Jin (Dept. of Biology, Kangwon National Univ.)
Kwon, Sera (Dept. of Biology, Kangwon National Univ.)
Choia, Woo-Jin (Dept. of Biology, Kangwon National Univ.)
Cho, Han-Na (Dept. of Biology, Kangwon National Univ.)
Park, Daesik (Division of Science Education, Kangwon National Univ.)
Publication Information
Korean Journal of Environment and Ecology / v.30, no.4, 2016 , pp. 694-704 More about this Journal
Abstract
Various infectious and parasitic diseases are known to be the main factors that cause decline in the global amphibian population. In Korea, commercial frog farms have been running since 2005. However, until now, studies on diseases including studies on parasitic diseases that occur in farm frogs have not been conducted. In this study, we studied and compared the acanthocephalan infection rates and the number of parasites in the body cavity, stomach, and small and large intestines of the Dybowskii's brown frogs collected from inside and outside the frog farms in Inje, Goesan, Gongju and Boryeong. In addition, we classified the acanthocephalan parasites into genera by analyzing their nuclear 18S rRNA genes. On an average, 51.7% of the investigated frogs were infected by acanthocephalans, which belong to the Centrorhynchus genus. The infection rate of the frogs collected in the Inje farm was 15%, significantly lower than those from the Goesan, Gongju and Boryeong farms. The rate in Goesan was 55%, which is lower than Gongju (80%) and Boryeong (90%) although it is not statistically significant. No difference was found in the infection rate and in the number of parasites in male and female frogs and between the groups collected from inside and outside of the farms. The number of infected parasites negatively correlated with the body condition of the frogs. The most parasites were found in the stomach followed by the small and large intestines and the body cavity. This study is the first report on the Centrorhynchus acanthocephalan infection of amphibians in Korea and it suggests the necessity for acanthocephalan parasite management and for conducting further disease-related studies in commercial frog farms.
Keywords
AMPHIBIAN; DISEASE; PARASITE; REARING; FROG; CLASSIFICATION;
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Times Cited By KSCI : 8  (Citation Analysis)
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1 Amin, O.M. (2013) Classification of the acanthocephala. Folia Parasit. 60(4): 273-305.   DOI
2 Amin, O.M., R.A. Heckmann and N. van Ha (2014) acanthocephalans from fishes and amphibians in Vietnam, with descriptions of five new species. Parasite 21: 53, doi: 10.1051/parasite/2014052.   DOI
3 Amin, O.M., R.A. Heckmann, E. Wilson, B. Keele and A. Khan (2015) The description of Centrorhynchus globirostris n. sp. (Acanthocephala: Centrorhynchidae) from the pheasant crow, Centropus sinensis (Stephens) in Pakistan, with gene sequence analysis and emendation of the family diagnosis. Parasitol. Res. 114: 2291-2299   DOI
4 Bataille A., J.J. Fong, M. Cha, G.O.U. Wogan, H.J. Baek, H. Lee, M.S. Min and B.W. Waldman (2013) Genetic evidence for a high diversity and wide distribution of endemic strains of the pathogenic chytrid fungus Batrachochytrium dendrobatidis in wild Asian amphibians. Mol. Ecol. 22: 4196-4209   DOI
5 Bayoumy, M.E., O.K. Abd El-Dady and H.A.M. Osman (2006) Site adaptations of Acanthogyrus (Acanthosentis) tilapiae: observations through light and scanning electron microscopy. J. Vet. Sci. 7: 339-342   DOI
6 Bhattacharya, S.B (2007) Handbook on Indian Acanthocephalan. Zoological Survey of India, Calcuta Repor Graphics, Kolkata, India.
7 Daszak, P., L. Berger, A.A. Cunningham, A.D. Hyatt, E. Green and R. Speare (1999) Emerging infectious diseases and amphibian population declines. Emerg. Infect. Dis. 5: 735-748   DOI
8 Bush, S.E., D.W. Duszynski and B.B. Nickol (2009) Acanthocephala from amphibians in China with the description of a new species of Pseudoacanthocephalus (Echinorhynchida). J. Parasitol. 95(6): 1440-1445   DOI
9 Choi, C.J., H.J. Lee, J.H. Go, Y.K. Park, J.Y. Chai and M. Seo (2010) Extraintestinal migration of Centrorhynchus sp. (acanthocephala: Centrorhynchidae) in experimentally infected rats. Korean J. Parasitol. 48(2): 139-143.   DOI
10 Crompton, D.W,T. and B.B. Nickol (1985) Biology of the Acanthocephala. Cambridge University Press, New York, USA.
11 Densmore, C.L. and D.E. Green (2007) Diseases of amphibians. IALR J. 48(3): 235-254   DOI
12 Kim, K.H., S.J. Ahn, S.J. Kim and H.C. Park (2013) Effect of temperature on mass mortality of the larval toad (Bufo bufo gargarizans) in Mangwel Pond. Korean J. Environ. Biol. 32(4): 340-346.(in Korean with the English abstract)
13 Jeon, S.G. (2006) Fish Parasitology. SusanTimes, Seoul.(in Korean)
14 Jeong, Y.J., J.T. Kim and G.H. Suh (2014) Case report: mass death of frogs (Rana dybowskii) caused by septicemia in artificial raising farm. Korean J. Vet. Serv. 37(3): 203-212   DOI
15 Kang, H.M., H. Lee, S.G. Yim and Y.D. Kim (2013) Histopathology of a acanthocephalan infection in swamp eel, Monopterus albus. J. Fish. Pathol. 26(3): 303-310 (in Korean with the English abstract)   DOI
16 Kim, D.J. (1998) Bolbosoma koreana n. sp. from salmon (Oncorhynchus masou var masou) in Korea. MS thesis, KyungHee University, Seoul, Korea.(in Korean with the English abstract)
17 Kim, K.H. (1993) Analysis of distribution pattern of parasitic helminths of anurans in Korea and effects on the density of host population. Korean J. Environ. Biol. 11: 110-115(in Korean with the English abstract)
18 Kim, J.T., J.Y. Park, H.S. Seo, H.G. Oh, J.W. Noh, S.W. Kim and H.J. Youn (2001) Identification of acanthocephala discovered in changran-pickles and myungran-pickles. J. Ver. Sci. 2: 111-114.
19 Kim, S., A.H. Eom, D. Park and N.Y. Ra (2008) Detection of infectious fungal diseases of frogs inhabiting in Korea. Mycobiology 36(1): 10-12   DOI
20 Kim, S., M.Y. Sim, A.H. Eom, D. Park and N.Y. Ra (2009) PCR detection of ranavirus in Gold-spotted pond frogs (Rana plancyi chosenica) from Korea. Korean J. Environ. Biol. 27(1): 110-113
21 Petersen, C.E., R.E. Lovich, C.A. Phillips, M.J. Dreslik and M.J. Lannoo (2016) Prevlaence and seasonality of the amphibian Chytrid fungus Batrachochytrium dendrobatids along widely separated longitudes across the United States. EcolHealth Doi: 10.1007/s10393-016-1101-4.   DOI
22 Sim, H.S., J.K. Kim, D.I. Kim and D. Park (2012) Ecological characteristics of leech ectoparasitism on mountain frogs in Odaesan National Park. Korean J. Herpetol. 4: 1-7
23 Tkach, V.V., O.I. Lisitsyna, J.L. Crossley, T.T. Binh and S.E. Bush (2013) Morphological and molecular differentiation of two new species of Pseudoacanthocephalus Petrochenko, 1958 (Acanthocephala: Echinorhynchidae) from amphibians and reptiles in the Philippines, with identification key for the genus. Syst. Parasitol. 85: 11-26
24 Yang, H.J., H.J. Baek, R. Speare, R. Webb, S.K. Park, T.H. Kim, K.C. Lasater, S.P. Shin, S.H. Son, J.H. Park, M.S. Min, Y.J. Kim, K. Na, H. Lee and S.C. Park (2009) First detection of the amphibian chytrid fungus Batrachochytrium dendrobatidis in free-ranging populations of amphibians on mainland Asia: survey in South Korea. Dis. Aquat. Org. 86: 9-13   DOI
25 Yu, J.D. (1986) A study on the acanthocephala (Pallisentis cleatus van cleave, 1928) in Monopterus albus Z. MS thesis, Chonbuk National University, Jeonju, Korea.(in Korean with the English abstract)
26 Jeong, A., H.J. Yang, H.J. Baek, Y.M. Ko, H. Lee and M.S. Min (2010) Distribution of the amphibians infected by chytrid fungus (Bd) in South Korea. Korean J. Herpetol. 2: 9-15.
27 Park, D., M.S. Min, K.C. Lasater, J.Y. Song, J.H. Suh, S.H. Son and R.H. Kaplan (2014) The conservation of amphibians in Korea. In: H. Heatwole, I. Das(eds.), Conservation Biology of Amphibians of Asia. Natural History Publications Sdn. Bhd. Malaysia.
28 Park, G.M. (2012) Pathogenic parasites of Korean wild reptiles and amphibians: a study of implications for pathogen transmission in a changing climate. Korean J. Nat. Conserv. 6(2): 94-101 (in Korean with English abstract)   DOI
29 Petrochenko, V.I. (1971) Acanthocephala of Domestic and Wild Animals. US Department of Agriculture and National Science Foundation, Washington DC, USA.
30 Posada, D. and K.A. Crandall (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14: 817-818.   DOI
31 Rachowicz, L.J., R.A. Knapp, J.A.T. Morgan, M.J. Stice, V.T. Vredenburg, J.M. Parker and C.J. Briggs (2006) Emerging infectious disease as a proximate cause of amphibian mass mortality. Ecology 87: 1671-1683.   DOI
32 Haustein, T., M. Lawes, E. Harris and P.L. Chiodini (2010) An eye-catching acanthocephalan. Clin. Microbiol. Infec. 16(6): 787-788   DOI
33 Sahar, M.M., T.A. Madani, I.Z. Al Mohsen and E.L. Almodovar (2006) A child with an acanthocephalan infection. Ann. Saudi. Med. 26: 321-324.   DOI
34 Shin, D.W. and U.T. Shim (1984) Studies on the cystacanth of acanthocephala from the mesentery of Natrix tigrina. Chungnam Med. J. 11(1): 23-33. (in Korean with the English abstract)
35 Siegel, S. and N.J. Castellan (1988) Nonparametric Statistics for the Behavioral Sciences(2nd ed.). McGraw-Hill, New York.
36 Ducatez, S. and R. Shine (2016) Drivers of extinction risk in terrestrial vertebrates. Conserv. Lett. DOI:10.1111/conl.12258   DOI
37 Fong, J.J., T.L. Cheng, A. Bataille, A.P. Pessier, B. Waldman and V.T. Vredenburg (2015) Early 1900s detection of Batrachochytrium dendrobatidis in Korean amphibians. PLoS ONE 10(3): e0115656. doi: 10.1371/journal.pone.0115656   DOI
38 Hong, S.T., S.H. Lee, J.Y. Chai and B.S. Seo (1985) A study on the infection status of tadpoles and frogs by the metacercariae of Fibricola seoulensis in Korea. Korean J. Parasitol. 23(1): 73-78   DOI
39 Huelsenbeck, J.P. and F. Ronquist (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17: 754-755   DOI
40 IUCN Red List (2014) Http://www.iucnredlist.org/initiatives/amphibians/analysis.
41 Mazzoni, R., A.A. Cunningham, P. Daszak, A. Apolo, E. Perdomo and G. Speranza (2003) Emerging pathogen of wild amphibians in frogs (Rana catesbeiana) farmed for international trade. Emerg. Infect. Dis. 9(8): 995-998   DOI
42 Kim, S.R., J.S. Lee, J.H. Kim, M.J. Oh, C.S. Kim, M.A. Park and J.J. Park (2011) Fine structure of Longicollum pagrosomi (Acanthocephala: Pomphorhynchidae) and intestinal histopathology of the red sea bream, Pagrus major, infected with acanthocephalans. Parasitol. Res. 109: 175-184.   DOI
43 Larkin, M.A., G. Blackshields, N.P. Brown, R. Chenna, P.A. McGettigan, H. McWilliam, F. Valentin, I.M. Wallace, A. Wilm, R. Lopez, J.D. Thompson, T.J. Gibson and D.G. Higgins (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23(21): 2947-2948.   DOI
44 Lee, J.H. and D. Park (2016) Encyclopedia of Korean Amphibians. Nature and Ecology, Seoul, Korea.
45 Mehlhorn, H. (2008) Encyclopedia of Parasitology(3rd ed.). Springer, Germany.
46 Miller, D.L., M.J. Gray, S. Rajeev, A.C. Schmutzer, E.C. Burton, A. Merrill and C.A. Baldwin (2009) Pathologic findings in larval and juvenile anurans inhabiting farm ponds in Tennessee, USA. J. Wildlife Dis. 45(2): 314-324   DOI
47 Ministry of Environment. (2005) Wildlife Animal and Plant Protection Law: Article # 14 (1-3). (in Korean)
48 Najiah, M., S.W. Lee, W. Wendy, L.W. Tee, M. Nadirah and S.H. Faizah (2009) Antibiotic resistance and heavy metals tolerance in gram-negative bacteria from diseased American bullfrog (Rana catesbeiana) cultured in Malaysia. Agric. Sci. China 8(10): 1270-1275   DOI
49 Near, T.J., J.R. Garey and S.A. Nadler (1998) Phylogenetic relationships of the acanthocephala inferred from 18S ribosomal DNA sequences. Mol. Phylogenet. Evol. 10:287-298   DOI