• Korean Journal of Plant Tissue Culture
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• v.24 no.5
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• pp.305-311
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• 1997

Bacillus thuringiensis, a gram-positive soil bacterium, is characterized by its ability to produce crystalline inclusions during sporulation. The crystal proteins exhibit a highly specific insecticidal activity. An insecticidal crystal protein (ICP), Cry II A, is specifically toxic to both lepidopteran and dipteran insects. In this study, tobacco plants transformed by the cry II A gene have been generated. The Cry II A crystal protein was purified from E. coli JM103 harboring cry II A gene by differential solubility. The activated Cry II A was prepared by tryptic digestion. The purified protoxin (70 kDa) and the activated toxin (50 kDa) were analyzed by SDS-PAGE. To generate the transgenic tobacco having cry II A gene, the cry II A gene was subcloned to a plant expression vector, pSRL2, having two CaMV 35S promoters. The recombinant plasmid was transformed into tobacco (N. tabacum var. Petit Havana SR1) by Agrobacterium-mediated leaf disc transformation. Through the regeneration, six putative transgenic tobacco plants were obtained and three transformants were confirmed by Southern blot analysis. It has been found that one plant had single copy of cry II A gene, another had two copies of the gene, and the third had a truncated gene. After the immunochemical confirmation of cry II A expression in plants, the transgenic tobacco plants will be used to study the genetics of future generation with the insecticidal crystal protein gene cry II A.

• Journal of Korean Society of Forest Science
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• v.98 no.3
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• pp.339-350
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• 2009

This study was carried out to investigate distributional condition of rare plants and useful plant resources, and to verify distributional patterns of understory vegetation associated with the upper layer's vegetation structure. Total 59 families, 160 genera, 218 kinds of vascular plants were identified at the study site including 6 rare plants designated by Korea Forest Service (Lloydia triflora Bak., Trillium kamtschaticum Pall., Lilium distichum Nakai, Anemone koraiensis Nakai, Iris odaesanensis Y.N. Lee, Viola diamantica Nakai). Twenty three species of useful plant resources were also identified at the site; 8 of them showed clustered distributions and the others were prone to scatter. Actual vegetation of this study area consisted of one natural community dominated by Quercus mongolica Fisch. and three disturbed communities of Larix kaempferi (Lamb.) Carriere, Abies holophylla Max. and/or a herbaceous vegetation resulting from forest removal and strong wind of mountain top. This classification was strongly supported by cluster analysis based on the surveyed plot data. Distributional patterns of understory vegetation within forest stand were somewhat related to overstory vegetation structure, but showed a different tendency according to site condition, species composition, and competitive pressure among understory vegetation. Therefore, in order to protect the important understory components as forest genetic resources, forest treatments such as density control of overstory should be implanted based on understanding of impact on understory's dynamics and growing condition.

• Korean journal of communication and information
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• v.44
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• pp.7-43
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• 2008

This study aims to identify a new direction in the consumer-brand relationship by quantitatively approaching from the psychological aspect consumers' brand love, which is the most powerful strategic element in forming an emotional consumer-brand relationship. What significance does brand love have in a consumer-brand relationship? Brand love is the most fundamental in the emotional consumer-brand relationship. Through brand ownership, consumers add meaning to their lives, and love brands by expressing themselves. Love is a scale that understands consumer-brand relationship from the most interpersonal aspect and can be considered as the most essential element in an empathetic relationship. Consumers' brand love not only determines brand image and reliability but through brand loyalty, it also influences market competitiveness. In addition, as a medium that can overcome brand crisis, it serves as a turning point of the relationship and can enhance the strength of consumers' brand love. Under such generational and theoretical background, this study took a psychological approach on love in emotional consumer-brand relationship. Focusing on the most representative theory and classification of love, Sternberg's Triangular Theory of Love, types of brand love were examined. 552 questionnaires were distributed through a survey with mobile phone service and product brands, and a factor analysis was conducted. Types of brand love revealed through the results of this study were very similar to Sternberg's types of love, and it was found that the types of brand love varied based on product category. It can be suggested that the concept of brand love is an important strategic idea in brand communication.

• Journal of Korean Home Economics Education Association
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• v.31 no.3
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• pp.57-71
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• 2019

This study intended to analyze the ethical clothing consumption behavior of female adolescents and use it as a basic data for education. Specifically, the female adolescents were classified according to their shopping orientation and then the differences among these groups were examined in terms of their ethical consumption behavior of clothing products, i.e., buying, using and disposing. As a result, three groups were identified: pleasure-seeking, intermediate, independence pursuing according to the shopping orientation sub-factors (loyal, enjoyment, impulsive, imitative and independent). The pleasure-seeking group were more likely to conduct ethical use behavior of clothing products, while the independence-pursuing group conducted more ethical buying and ethical disposing behavior. The lower their desire to enjoy shopping itself, the more cautious they are about their own decisions, and the more confident they about buying from the brand and store they liked, the more likely they were to conduct ethical buying behavior of clothing products. On the other hand, when the emotional and desire-seeking tendencies are combined with independent shopping tendencies, the more likely they conduct ethical use behaviors. In addition, the more they make independent purchasing decisions, the more likely they are to conduct ethical disposal behaivors. The results of this study suggest that providing detailed consumer education that considers individual differences in shopping orientation is needed.

• Journal of Digital Convergence
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• v.18 no.10
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• pp.459-467
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• 2020

The number of SNS false and exaggerative advertising has been consistently increasing nowadays. Among others, millennials who use SNS most frequently and enjoy e-commerce have become victims of false and exaggerative advertising. Thus, this study is designed to examine millennial consumers' attitude toward SNS false and exaggerative advertising through in-depth interview. Study findings suggest that millennials have very negative attitude toward SNS false and exaggerative advertising regardless of if they are victims or not. In particular, millennials who are victims of SNS false and exaggerative advertising have negative attitude toward SNS companies as well as advertised brands on SNS. Millennial consumers think that SNS companies need to come up with guidelines to regulate SNS false and exaggerative advertising, and government also needs to apply proper measures. Since SNS false and exaggerative advertising may have negative impacts on millennials' purchase intentions as well as brand loyalty, companies need to consider millennial consumers' characteristics when it comes to launching SNS advertising targeting millennials. This finding provides practical implications for marketers.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.33 no.2
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• pp.549-557
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• 2013

An flood inundation map is able to convey spatial distribution of inundation to a decision maker for flood risk management. A roughness coefficient with unclear values and a discharge obtained from the stage-discharge rating equation are key sources of uncertainty in flood inundation mapping by using a hydraulic model. Also, the uncertainty analysis needs an observation for the flood inundation, and satellite images is useful to obtain spatial distribution of flood. Accordingly, the objective of this study is to quantify uncertainty arising roughness and discharge in flood inundation mapping by using a hydraulic model and a satellite image. To perform this, flood inundations were simulated by HEC-RAS and terrain analysis, and ISODATA (Iterative Self-Organizing Data Analysis) was used to classify waterbody from Landsat 5TM imagery. The classified waterbody was used as an observation to calculate F-statistic (likelihood measure) in GLUE (Generalized Likelihood Uncertainty Estimation). The results from GLUE show that flood inundation areas are 74.59 $km^2$ for lower 5 % uncertainty bound and 151.95 $km^2$ for upper 95% uncertainty bound, respectively. The quantification of uncertainty in flood inundation mapping will play a significant role in realizing the efficient flood risk management.

• Culinary science and hospitality research
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• v.19 no.5
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• pp.206-224
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• 2013

The purpose of this study was to examine the formation of customer's repeated use that is real long-term commitment in franchise/chain coffee shops. 457 effective samples were analyzed using PASW 18.0 and AMOS 18.0. The results of the analysis of the relationships between user experience, customer satisfaction, customer commitment and repeated use are as follows. First, it was presented that atmosphere, price fairness and coffee product quality had a positive impact on customer satisfaction. Second, customer satisfaction had a positive impact on customer commitment and repeated use. Finally, there was the mediating effect of continuous commitment between customer satisfaction and repeated use. Based on the results of the above analysis, the managerial implications for increasing repeated use of coffee shops are as follows. First, the strategy for enhancing performance of user experience is to try to improve atmosphere(facilities, ambient/lighting), price fairness, coffee product quality(taste, flavor, temperature). Second, the strategy for forming repeated use is to enhance customer commitment(affective commitment, continuous/normative commitment).

• Sijohaknonchong
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• v.19 no.1
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• pp.207-234
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• 2003

The purpose of this research paper is to study the characteristics of Huang Yunseok's sijo and it's implications for his poetic intentions. Huang Yunseok's sijo is didactic in its presentation of facts and ethical lessons. The two main facts it presents are royal favors and his clan's pride. Royal favors concern. for example, encouragement of the king, the king's birthday and deathday, and securing a government position by the king's order. His clan's pride concerns, for example, the geographically wonderful place of Huang Yunseok's ancestor's tomb, his ancestor's great conduct, his clan's beautiful tradition, his birth place, and his birth dream. The main content of the ethical lessons concerns cultivation of the human mind and practicing Confucian ethics. Examples of the former describe the purpose of Confucian study, the ethical doctrine that human being's inborn nature is good, the character of the human mind, and the method of cultivating the human mind. Examples of the latter describe the moral rules to govern the Five Human Relations, separation between husband and wife, and discrimination between males and females. The poetic intention of Huang Yunseok's sip consists of political and economic self-promotion and theoretical devotion to Confucian ideology. Proud of his educated, high Confucian clan, he tries to get self-promotion politically and economically by vowing loyalty to the king. He implies devotion to the Confucian ideology through his description of the practice of the Confucian moral rules, the clinging to traditional Neo-Confucianism, and his insistence on the Neo-Confucian theory that all human beings and animals are the same in their original nature. In conclusion, the didactic character of Huang Yunseok's sip stems from his intention to promote himself politically and economically and his theoretical devotion to Confucian ideology. The way of indicative expression originates from the didactic character of Huang Yunseok's sijo.

• Journal of Korea Water Resources Association
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• v.39 no.5 s.166
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• pp.431-440
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• 2006

Recently, water conflicts are rapidly increasing and more serious. So, it needs to establish the reasonable water allocation rules and criteria. This study aims at performing inter-sectoral water allocation with a focus on economic effciency and social utility. To allocate water among the sectoral water uses, water shortage damage functions were estimated and then converged to the utility functions. Finally, each sectoral water uses are allocated by applying 'law equimarginal utility' to maximize social utility. Also weighting factor which reflects scale and characteristic of water demand in a region was estimated to perform the inter-regional water allocation. The water allocation rule was applied to the future water-deficit situation in Han-river basin. As a result, domestic water use was allocated more sufficient agricultural and industrial water use. Also, the water shortage occurs severely in the rural area like Gangwon-do because of its low urbanization and industrialization. This study suggests a alternative view of the economic water allocation which have difficulty under water supply mechanism in Korea.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.11
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• pp.8096-8105
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• 2015

The purpose of this research is to understand influence of personal turnover factors and work turnover factors on job satisfaction in career decision-making and to analyze the moderating effects of supervisor support in their relations. Meaningful difference between one group with high supervisor support and other group with low supervisor support is demonstrated in regard with a course; job burnout ${\rightarrow}$ job satisfaction, and work overload ${\rightarrow}$ job satisfaction. Hence from a perspective of the course of job burnout ${\rightarrow}$ job satisfaction and work overload ${\rightarrow}$ job satisfaction, it can be argued that a group with low supervisor support yields more influence. Also it is shown that conflicts on balancing between job and family do not have influence on job satisfaction for either group with high supervisor support or with low supervisor support. As the result of the research shows, it bears a quite significant meaning that career development/lack of opportunity out of personal turnover intentions disclose difference between these two groups with different levels of supervisor support. Especially, a group of flight attendants which females are consisted of a majority of employees would require that their supervisors have more emotional interactions with each employee, so as that better opportunity would be given to the employees. To this end, utmost efforts are needed that deep and genuine relations with a supervisor can be built up.