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포기거머리말(Zostera caespitosa Miki, 거머리말과)의 생물지리학적 연구: I. 서식환경과 형태적인 특징

  • Lee, Sang-Yong;Kwon, Chun-Joong;Min, Won-Ki;Heo, Seung;Choi, Chung-Il
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.05a
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    • pp.406-407
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    • 2000
  • 한반도에 서식하는 해초(Seagrass) 중 거머리말속(Zostera, Zosteraceae)은 거머말(Zostera marina), 애기거머리말(Z. japonica), 왕거머리말(Z. asiatica), 수거머리(Z. caulescens), 포기거머리말(Z. caespitosa) 5종이 기재되었다 (Miki, 1932). 이 해들은 연안의 사질 또는 사니질에 서식하며 생태계에 중요한 역할을 담당하고 있다. 그러나 연안의 매립과 개발로 인해 이들 해초지의 감소와 연안생태계의 붕괴가 진행되고 있으나 해초에 대한, 특히 Zostera속에 대한 연구는 미진하다.(중략)

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Production and Purification of Alkaline Protease from Bacillus sp. CW-1121 (Bacillus sp. CW-1121이 생성하는 Alkaline Proteas의 생산 및 정제)

  • Lee, Woo-Je;Son, Gyu-Mok;Choi, Cheong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.4
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    • pp.388-394
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    • 1991
  • Alkaline protease producing bacteria were isolated from soil and identified as Bacillus sp. CW-1121. It was found that the production of alkaline protease reached to maximum in 5 day of fermentation at 4$0^{\circ}C$. The enzyme was purified by ammonium sulfate precipitation, gel filtration on Sephadex G-150 and DEAE-cellulose ion-exchange chromatography. The homogeneity of the purified enzyme was verified by polyacrylamide gel electrophoresis. The enzyme was purified 5.72 fold and yield of the enzyme purification was 16.71%. When the purified enzyme was applied to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the molecular weight was estimated to be 55, 000.

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Effect of Acanthopanacis Cortex Boiling Extract Solutions on Fat Accumulation in the Obese Rats Induced by High Fat Dietary (오갈피의 열수추출액이 고지방식이에 의한 비만유도 흰 쥐의 지방 축적에 미치는 영향)

  • Sung, Tae-Soo;Son, Gyu-Mok;Bae, Man-Jong;Choi, Cheong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.1
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    • pp.9-16
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    • 1992
  • This study was carried out to evaluate the effect of Acanthopanacis Cortex boiling extract solutions on the fat accumulation in the obese rats induced by the oral high fat administration for six weeks. Total cholesterol, neutral fat and adipose acid of ACR groups were lower than the control group. During the feeding experiment, LDL and VLDL were increased while HDL was decreased in all groups. Insulin and cortisol were higher than the control group, due to the fat accumulation. Based on the above results, it was shown that it is possible to improve fat accumulation induced by high fat dietary through using the oral administration of Acanthopanacis Cortex boiling extract solutions.

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Image Segmentation and Coding Using Edge Tracing (에지추적에 의한 영상 분할 및 부호화)

  • Choi, Cheong;Lee, Sang-Mi;Kim, Nam-Chul;Son, Hyon
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.26 no.3
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    • pp.105-112
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    • 1989
  • A new simple edge-based segmentation method composed of edge tracing, region filling, and post processing is proposed. Solving so called the small gap problem common to most of edge-based methods, this method segments images so completely as to be suitable for image coding. Experimental results show that our methods has much less (1/3) computation time than Perkins' one, and its reconstructed images is good on visual perception.

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Organic Acids and Volatile Flavor Compounds in Traditional Andong Sikhe (전통 안동식혜의 유기산 및 향기성분)

  • Woo, Hi-Seob;Choi, Cheong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.2
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    • pp.208-213
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    • 1995
  • Andong Sikhe in Korea was prepared and fermented at 5$^{\circ}C$ and the taste and flavor compounds were evaluated. Major flavor components were identified by gas chromatography-mass spectrometer as camphene, sabinene, 1-(1, 5-dimethyl-4-hexyl)-4-methyl-benzene, alpha-zingibirene, farnesene, 2, 6-bis(1, 1-dimethylethyl)-4-metethyl-phenol, beta-sesquiphellandrene, calalene, tetradecanoic acid, and 9, 12-octadecanoic acid. The concentration of nonvolatile organic acid such as lactic acid, oxalic acid and citric acid were 18.10mg/100g, 1.04mg/100g and 1.37mg/100g, respectively, and those of other nonvolatile organic acid were a little. The pH and acidity of Andong Sikhe were 4.06 and 0.32 during fermentation and storage.

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한국재래메주에서 분리한 Scopulariopsis brevicaulis가 생성하는 Protease의 특성 및 작용양상

  • Choi, Cheong;Choi, Kwang-Soo;Kim, Sung;Lee, Seon-Ho;Son, Jun-Ho;Choi, Hee-Jin;Lee, Sang-Seon;An, Bong-Jeon
    • Microbiology and Biotechnology Letters
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    • v.25 no.1
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    • pp.56-61
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    • 1997
  • An alkaline protease producing microorganism was isolated from korean traditional Meju and identified as Scopulariopsis brevicaulis. The optimum culture condition of Scopulariopsis brevicaulis for the production of alkaline protease was as follow: 2% soluble starch, 0.2$, tryptophan, 0.1% (NH$_{4}$) $_{2}$S$_{2}$O$_{8}$ 0.2% NaHPO$_{4}$, pH 7.5, 35$\CIRC $C. The optimum pH and temperature for the enzyme activity of alkaline protease producing Scopulariopsis brevicaulis were pH 9.0 and 50$\circ $C, respectively. The enzyme was relatively stable at pH 6.0~11.0 and at temperature below 40$\circ $C. The activity of the enzyme was inhibited by Hg$^{2+}$ whereas Cu$^{2+}$ gave rather activating effects on the enzyme activity. Phenylmethanesulfonyl fluoride inhibited the enzyme activity. This result indicates that serine is very important role in this enzyme. Km value for casein was 1.2410$^{4}$ M/L, V$_{max}$ value for casein was 25.99 $\mu $g/min. This enzyme hydrolyzed casein more rapidly than the hemoglobin.

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Inhibition Effect against Tyrosinase of Condensed Tannins from Korean Green Tea (한국산 녹차로부터 분리한 축합형 탄닌의 tyrosinase 저해효과)

  • Kim, Jin-Ku;Cha, Woen-Seup;Park, Joon-Hee;Oh, Sang-Lyong;Cho, Young-Je;Chun, Sung-Sook;Choi, Cheong
    • Korean Journal of Food Science and Technology
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    • v.29 no.1
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    • pp.173-177
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    • 1997
  • For the utilizing of tannins in the functional foods and natural inhibitor against browning reaction by tyrosinase in foods, inhibition effect against tyrosinase of tannins from Korean green tea was determined. Acetone extract from Korean green tea showed inhibition effect against tyrosinase. The gallocatechin compounds showed higher inhibition effect than the catechin compounds. In terms of stereo isomers, (-)-epicatechin compounds had higher inhibition effect than the (+)-catechin compounds. The monomer had higher inhibition effect than the dimer.

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Inhibitory Effect of Condensed Tannins Isolated from Korean Green Tea against Xanthine Oxidase (한국산 녹차로부터 분리한 축합형 탄닌의 Xanthine Oxidase 저해효과)

  • Cho, Young-Je;Chun, Sung-Sook;Choi, Cheong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.4
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    • pp.418-422
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    • 1993
  • For the purpose of utilizing tannins in the functional foods and crude drugs the xanthine oxidase inhibition of tannins isolated from Korean green tea was determined. Acetone extract from Korean green tea showed inhibitory effect against the xanthine oxidase. The galloyl tannins showed higher inhibitory activity against xanthine oxidase than the nongalloyl tannins. In terms of stereo isomers, (-)-epicatechins had higher inhibitory activity than the (+)-catechins. The synergistic activity was also observed. Tannins isolated from Korean green tea appeared to be incompetitive inhibitor against the xanthine oxidase.

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Studies on Artificial Hatching of Hibernating Eggs, Bombyx mori L. (월년잠종의 인공부화에 관한 연구)

  • 김윤식;손해용
    • Journal of Sericultural and Entomological Science
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    • v.18 no.1
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    • pp.7-10
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    • 1976
  • This experiments were carried out to know hatching power on treatments of cold temperature and hydrochioric acid Iron Late January to Early February. Used silkworm variety was Jam 103${\times}$ Jam 104 laying in Spring. The results may be summarized as follows: 1. The resting of silkworm eggs activated to Late January, to longer cold treatment was, the more effects of activating increased completely. So activated eggs were possible to incubation. 2. How to the light or short hours of hycrochloric acid were compare with heavy or long each other, it was inclined that the former seems to be worse than the latter. 3. On the interaction of cold treatments and hydrochloric acid, in case of short cold treatment, hatching power was better effect all treatments of hydrochloric acid than control, but no difference between specific gravity or treatment hours. On tile contrary, in case of more longer of cold treatments, hatching power depended not only upon the specific gravity, but also upon the treatment hours.

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Separation and Purification of Endo-polygalacturonase from Korean Jujube (한국산 대추로부터 Endo-polygalacturonase 분리 및 정제)

  • Choi, Cheong;Chun, Sung-Sook;Cho, Young-Je;Woo, Heui-Seob;Kim, Tae-Wan;Heo, Young-Hoon
    • Applied Biological Chemistry
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    • v.37 no.4
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    • pp.243-247
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    • 1994
  • Endo-polygalacturonase was purified from Jujube. The purification procedures included DEAE-cellulose ion exchange chromatography and gel filtration on Sephdex G-100. Enzyme was purified as a single protein band and purification yield was about 6%. When the purified enzyme was applied to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the molecular weight was estimated about 19,000. Purified enzyme formed hexagonal board type.

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