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Changes in lipid and Fatty Acid Composition in Korean Native Meju during Fermentation (한국 재래식 메주 발효과정에 있어서 지질 및 지방산 조성의 변화)

  • Son, Yang-Don;Choi, Chun-Un;An, Bong-Jeun;Son, Gu-Mok;Choi, Cheong
    • Applied Biological Chemistry
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    • v.28 no.4
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    • pp.226-232
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    • 1985
  • Changes in lipid and fatty acid composition in Korean native Meju were investigated at one week interval over 6 weeks of fermentation. For the systematically, salicic acid column chromatography and gas liquid chromatography were used. Following results were obtained. 1. The lipid fraction obtained from the soared soybean and the cooked soybean were mainly composed of $72.47%{\sim}92.35%$ of neutral lipid, phospholipid and glycolipid were 4.64% and 4.88%, respectively. During fermentation period, lipid content decreased to 80.59%, but glycolipid and phospholipid contents increased. 2. The triglyceride contents of nonpolar lipids prepared from the cooked soybean and the soaked sobean was 89.66% and 87.83% respectively. Free fatty acid, diglyceride and sterol contents increased during fermentation, whereas triglyceride content decreased. 3. Lipids extracted from the soaked soybean and the cooked soybean were composed of 54.58% linoleic acid, $22{\sim}20%$ oleic acid, $10{\sim}12%$ palmitic acid and $3{\sim}5%$ stearic acid. 4. During the Korean native Meju fermentation in palmitic acid decreased from the second week and stearic acid through $3rd{\sim}4th$ week. Oleic acid and linoleic acid content decreased gradually, but linolenic acid content increased. 5. During the fermentation, myristic acid content of glycolipid fraction increased. Lipase activity reached to the maximum at the 3rd week.

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Removal effect of Cadmium by Polyphenol Compound Extracted from Persimmon Leaves(Diospyros kaki folium) (감잎(Diospyros kaki folium)으로부터 분리한 Polyphenol 화합물의 카드뮴 제거효과)

  • Jo, Guk-Young;Choi, Hee-Jin;Son, Jun-Ho;Bae, Du-Kyung;Park, Mu-Hee;Woo, He-Sob;An, Bong-Jeon;Bae, Man-Jong;Choi, Cheong
    • Applied Biological Chemistry
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    • v.43 no.3
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    • pp.213-217
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    • 2000
  • In order to investigate the removal effect of cadmium by polyphenol compound extracted from persimmon leaves(Diospyros kaki folium), animal test was done. Two fractions such as F-1 and F-2 from persimmon leaves were compared with their safety and function. The food intake of group F-1 and F-2 considerably decreased within 1% level. Cadmium addition influenced to rat growth(a tested animal), but food efficiency ratio(FER) wasn't shown any considerable difference in F-1 and F-2, as compared to the control. Cadmium content of liver, kidney and femur considerably decreased within 1% level in F-1 and F-2, compared to the control, cadmium content of liver decreased 25% in F-1, 28% in F-2 3150 decreased 22% md 25% in kidney. In femur, also decreased 53% in F-1 and 59% in F-2 respectively. The test of cadmium content in feces indicate that the content considerably increased within 1% level in both group F-1 and F-2, as compared to the control(42% in F-1, 54% in F-2). As shown the above results, have seen the removal effect of cadmium by polyphenol compound.

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The Developmental Characterization of the Sericin jam(Bombyx mori), a Mutant in the Synthesis and Secretion of Fibroin (세리신잠(Nd-s. $Nd^H$의 발육 특성)

  • 김수연;손해룡
    • Journal of Sericultural and Entomological Science
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    • v.45 no.1
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    • pp.6-9
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    • 2003
  • This experiment was done to know developmental characteristics of Sericin jam. Incubation periods were 10 day 2 hr, and 10 day 1 hr. 11 day 1 hr for Nd-s jam, N $d^{H}$ jam, and Baegok jam, respectively. Hatching rates were 83.9, 83.3 and 96.0% for Nd-s jam, N $d^{H}$ jam, and Baegok jam. Larval periods were, 20 days 1 hr for Nd-s jam, 20 days 5 hrs for N $d^{H}$ jam, and 22 days 12 hrs for Baegok jam. Death rate of larvae was highest in N $d^{H}$ iam, followed by Nd-s jam and Baegok jam. Pupation rate was highest in Baegok Jam followed by Nd-s jam and that of N $d^{H}$ jam was the lowest among the three. Cocoon weight was 1.39, 1.08, and 2.01 g for Nd-s jam, N $d^{H}$ jam, and Baegok jam, respectively. Shell weight were 13, 3, and 48 cg for Nd-s jam, N $d^{H}$ jam, and Baegok jam. Cocoon shell ratios were 9.0% for Nd-s jam, 2.8% for N $d^{H}$ jam and 23.9% for Baegok jam. Cocoon sizes were 30.6${\times}$15.8 mm for Nd-s jam, 24.7${\times}$14.9 mm for N $d^{H}$ jam and 35.8 ${\times}$ 20.5 mm(1${\times}$w) for Baegok jam.w) for Baegok jam.

Phonology and Morphometrics Change of Zostera marina L. Population at Duksan Port in the Eastern Coast of Korea (동해 덕산항에 생육하는 거머리말(Zostera marina L.) 개체군의 생물계절학과 형태 변이)

  • 이상룡;이성미;김정하;최청일
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.8 no.2
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    • pp.70-77
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    • 2003
  • From March 1998 to August 2000, the phenological and morphometric changes of Zostera marina L. were examined at the Duksan Port in the eastern coast of Korea. Morphometric characteristics, phenological stage, shoot density, biomass of Z. marina population and environmental parameters were also measured. Nutrient levels in water column varied over the season. Morphometric characteristics of vegetative shoot changed with season; shoot heights ranged from 54.2 cm (March) to 100.0 cm (October). Reproductive shoots appeared from mid-March to early September of which the height was ranged from 97.8 cm (March) to 213.0 cm (July). The flowering phase started at 12$^{\circ}C$ and the fruit development was resulted up to 21$^{\circ}C$. The seed maturing was developed at 22$^{\circ}C$-$25^{\circ}C$. Shoot density and biomass in permanent quadrate (0.25 m$^2$) were significantly different among seasons ranging from 38 to 136 shoots (mean 80.3$\pm$6.5) for shoot density, and 190 g dry wt m$^{-2}$ in October 1998 to 922 g dry wt m$^{-2}$ in June 1998 for biomass respectively. Relationships between shoot morphometrics and physico-chemical parameters were not significantly correlated. Seasonal changes in water temperature seemed responsible for the replacement of reproductive phases and the annual changes of shoot morphometrics in Z. marina populations.

Qualities of Bread Added with Korean Persimmon(Diospyros kaki L.folium)Leaf Powder (한국산 감잎가루를 첨가한 빵의 품질)

  • Bae, Jong-Ho;Woo, Hi-Seob;Choi, Hee-Jin;Choi, Cheong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.5
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    • pp.882-887
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    • 2001
  • The purpose of this study was to investigated quality properties of breads prepared from wheat flour with persimon leaf power. Results showed that the volume of bread was lessened, the weight of bread went up, and baking loss rate diminished as persimmon leaf powder content incremented. The experiment showed that bread was maintained in the low level of springiness and cohesiveness was relatively reduced as time lapsed, whereas 3.0 and 5.0% test group remained significantly high level. There was a rapid increment in chewiness and gumminess, which tended to show significantly low as compared to control group, Results of sensory evaluation showed that the preference scores decreased as the persimmon leaf powder contents increased (p<0.05), flavor made no significant differences up to 3.0% test group, however, 5.0% test group containing elutionsuch as catechin or tannin produced sour and bitter taste so strongly that made if feel uncomfortable with. The augment of content indicated a low prederence in terms of texture, in an overall preference had no significant differences as compared to the control group, and 0.5% test group. Through the results of these of these experiments, we can conclude that the highest quality of persimmon leaf powder content is no more than 0.5% in making bread added with persimmon leaf powder.

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A Study on the Preparation of Traditional Andong sikhe with Lactic Acid Bacteria (유산균을 이용한 전통안동식혜의 제조방법에 관한 연구)

  • Choi, Cheong;Son, Gyu-Mok
    • Journal of the Korean Society of Food Culture
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    • v.7 no.3
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    • pp.259-270
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    • 1992
  • The changes in life style today appear many ways. Many housewives turn away from home preparation of the time consuming traditional foods, such as 'Andong sikhe'. The importance, however, of succeeding the traditional cuisines is getting appreciated widely nowadays. This study aimed to investigate the preparation of Andong sikhe by use of pure culture inoculation and the improvement of storage stability by the addition of stabilizers to the product. Lactobacillus delbreuckii was selected for the pure culture inoculation in the fermentation. The changes in chemical composition such as total acidity, sugar content, amino acid and various forms of nitrogen during fermentation were determined. The changes in pH of the product, the enzyme activities and the population of lactic acid bacteria were also followed in the process of fermentation. The Lactobacillus dominated in the beginning of the fermentation but the Streptococcus out numbered the former as the fermentation proceeded. The crude protein content increased up to the 4th day of fermentation but slowly decreased there after. The pH of the product rapidly decreased to 4.2 by the 2nd day of fermentation. The total acidity reached to the 0.38% by the 2nd day of fermentation and kept on increasing slowly during the fermentation. The free sugar consisted of 6 kinds including maltose and one unknown sugar. The amino form nitrogen increased up to 38.5mg% at the 2nd day of fermentation and the product tasted best at this time. The ammonia form nitrogen, water soluble and salt soluble protein decreased during fermentation. Proline and aspartic acid were the two major free amino acids. The free methionine increased while the free lysine decreased in the process of fermentation. The major amino acids of water soluble and salt soluble protein were glutamic acid and aspartic acid. The arginine content of salt soluble protein increased as the fermentation proceeded. Linoleic, palmitic and oleic acid were the three major fatty acids and occupy 90% or more of the total fatty acids. The activities of acid protease and liquefying amylase reached to the maximum at the 4th day of fermentation while those of saccharogenic amylase and lipase reached to the peak at the 2nd day of fermentation.

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Studies on the Development of Food Resources from Waste Seeds -II. Chemical Composition of Apple Seed- (폐기종실(廢棄種實)의 식량자원화(食糧資源化)에 관(關)하여 -제(第) 2 보(報) : 사과씨의 화학적(化學的) 조성(組成)-)

  • Yoon, Hyung-Sik;Choi, Cheong;Oh, Man-Jin
    • Korean Journal of Food Science and Technology
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    • v.15 no.2
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    • pp.128-132
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    • 1983
  • The apple seed contained 25.96% of crude fat and 37.62% of crude protein. The lipid fractions obtained by cilicic column chromatography were mainly composed of about 93.52% neutral lipid, whereas compound lipid was only 6.48% level. Among the neutral lipid separated by thin layer chromatography, triglyceride was 92.17%, sterol ester, sterol, diglyceride and free fatty acid were 3.53, 2.25, 1.44 and 0.56, respectively. The predominent fatty acids of total and neutral lipids were linoleic acid (59.79-69.37%) and oleic acid (20.04-29.82%), but those of glycolipid and phojspholipid were linoleic acid (29.20-36.04%). The major fatty acids of triglyceride separated from neutral lipid were oleic acid (44.31%), linoleic acid (36.66%) and palmitic acid (12.48%). The salt soluble protein of apple seed was highly dispersible in 0.02M sodium phosphate buffer containing about 1.0M $MgSO_4$, and the extractability of seed protein was 37%, Glutamic acid was the major amino acid in salt soluble protein, followed by arginine and aspartic acid. The eletrophoretic analysis showed three bands in apple seed protein, and the collection rate of the main protein fraction purified by Sephadex G-100 and G-200 was 76.6%. Glutamic acid, aspartic acid and arginine were the major amino acids of the main apple seed protein. The molecular weight for the main protein of the apple seed was estimated to be 45,000.

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Water Quality and Chlorophyll-a at the Birth Stage of a Large Reclaimed Estuarine Lake in Korea (Lake Hwaong) (간척하구호 (화옹호) 태동기의 수질과 엽록소-a 변화)

  • Kim, Ho-Sub;Chung, Mi-Hee;Choi, Chung-Il;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.36 no.4 s.105
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    • pp.455-462
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    • 2003
  • This study evaluated the change of water quality and chlorophyll - a at the birth stage of a large reclaimed estuarine lake (Lake Hwaong) of which the dike was finally constructed in March, 2002. Physico -chemical parameters and chlorophyll - a were investigated along a longitudinal transect, including 3 in-lake sites and 1 out-lake site from June to November, 2002. Salinity at all in-lake sites was over 21 psu during the study period, indicating that lake is still in the seawater phase. Salinity was periodically diluted at times when precipitation was high, especially in August. Chemocline was established in July near the dam site, and correspondingly vertical profile of dissolved oxygen was very clear during that Period. Total nitrogen and phosphorus concentrations at all lake sites were in the eutrophic range, and they were especially high at the stream inlet site. Nutrients concentration was not much varied vertically but significantly varied temporally, and correlated significantly with precipitation and chlorophyll-a concentration, indicating that inflowing water from the watershed seemed not to improve lake water by dilution but cause eutrophication of the lake, and thereby stimulate phytoplankton development. Based on the analyses of nutrient ratio (N/P) and trophic state deviation, both phosphorus and nitrogen appeared to limit phytoplankton growth in the lake. Phosphorus limitation appeared to be probable at all in-lake sites with being most severe at the stream inlet site. Nitrogen limitation seemed to occur at both in-lake and out-lake sites. These results indicate that in Lake Hwaong experiencing the very early stage of a reclaiming lake, water quality and phytoplankton development appear to be affect-ed largely by salinity and hydrology and nutrients from the inflowing water. Lake biogeochemistry is still very unstable, and thus further long-term study is necessary to understand the effects of seawater to freshwater conversion on lake biology and water chemistry.

A Fast Processor Architecture and 2-D Data Scheduling Method to Implement the Lifting Scheme 2-D Discrete Wavelet Transform (리프팅 스킴의 2차원 이산 웨이브릿 변환 하드웨어 구현을 위한 고속 프로세서 구조 및 2차원 데이터 스케줄링 방법)

  • Kim Jong Woog;Chong Jong Wha
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.42 no.4 s.334
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    • pp.19-28
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    • 2005
  • In this paper, we proposed a parallel fast 2-D discrete wavelet transform hardware architecture based on lifting scheme. The proposed architecture improved the 2-D processing speed, and reduced internal memory buffer size. The previous lifting scheme based parallel 2-D wavelet transform architectures were consisted with row direction and column direction modules, which were pair of prediction and update filter module. In 2-D wavelet transform, column direction processing used the row direction results, which were not generated in column direction order but in row direction order, so most hardware architecture need internal buffer memory. The proposed architecture focused on the reducing of the internal memory buffer size and the total calculation time. Reducing the total calculation time, we proposed a 4-way data flow scheduling and memory based parallel hardware architecture. The 4-way data flow scheduling can increase the row direction parallel performance, and reduced the initial latency of starting of the row direction calculation. In this hardware architecture, the internal buffer memory didn't used to store the results of the row direction calculation, while it contained intermediate values of column direction calculation. This method is very effective in column direction processing, because the input data of column direction were not generated in column direction order The proposed architecture was implemented with VHDL and Altera Stratix device. The implementation results showed overall calculation time reduced from $N^2/2+\alpha$ to $N^2/4+\beta$, and internal buffer memory size reduced by around $50\%$ of previous works.

Reproductive Phenology of Four Korean Seagrasses, Zostera caespitosa, Z. caulescens, Z. japonica and Z. marina (한국산 해초 포기거머리말, 수거머리말, 애기거머리말과 거머리말의 생물계절학)

  • Lee, Sung-Mi;Lee, Sang-Yong;Choi, Chung-Il
    • Ocean and Polar Research
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    • v.27 no.2
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    • pp.125-133
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    • 2005
  • This study described the phonology and reproductive potential of four species of Korean seagrasses, Zostera caespitosa, Z. caulescem, Z. Japonica and Z. marina. Z. caespitosa and Z. caulescens sampled from a mixed stand at the subtidal area of Yulpo Bay, Geojedo of the South Sea of Korea in November 2002 and August 2003. Z japonica and Z. marina occurred at the depth between the middle intertidal and shallow subtidal (<1m below mean sea level) of Seungbongdo (in Yellow Sea) samples collected in February and October 2003. The sexual reproductive phase of the four Zostera species was apparently different in timing of flowering, reproductive period, fruiting and seed maturing. Z. caespitosa flowered from February to early May $(10-16^{\circ}C)$, and its seed production completed in early May. The reproductive shoots of Z. caulescens began to appear in January $(9^{\circ}C)$, and its flowering followed from February to June $(10-19^{\circ}C)$. The flowers of Z. japonica were observed from July to September $(18-22^{\circ}C)$, and its seeds matured from August to September. The most commonly I marina flowered from April to August $(7-21^{\circ}C)$ and developed into seeds in July. Z. caulescens, the largest plant, had the highest number of seeds per shoot and longest spadix length. Z. marina, which was intermediate In size, recorded the highest reproductive potential. The study indicates that the reproductive phase and potential of the four species of seagrass from Korea are highly related to water temperature, and the populations of these species show a perennial lifespan with a low sexual reproductive input.