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Physiological Characteristics and Anti-Obesity Effect of Enterococcus faecalis MD366 isolated from Raw Milk (원유에서 분리한 Enterococcus faecalis MD366의 생리적 특성 및 비만 억제 효과)

  • Park, Sun-Young;Cho, Seong-A;Han, Noo-Ri;Lim, Sang-Dong
    • Journal of Dairy Science and Biotechnology
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    • v.32 no.2
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    • pp.147-156
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    • 2014
  • The aim of this study was to investigate the physiological characteristics and anti-obesity effects of E. faecalis MD366 isolated from raw milk. E. faecalis MD366 inhibited lipase activity ($65.0{\pm}0.9%$) and differentiation of 3T3-L1 adipocytes ($27.4{\pm}1.4%$) at a concentration of $100{\mu}g/mL$ ($10^8CFU/g$ E. faecalis MD366). The optimum growth temperature of E. faecalis MD366 was $37^{\circ}C$. Among 16 tested antibiotics, E. faecalis MD366 demonstrated the highest sensitivity to novobiocin and the highest resistance to neomycin, kanamycin, and vancomycin. The strain also showed high acid phosphatase activity. Moreover, E. faecalis was relatively tolerant to bile juice and acid, and displayed high resistance to Escherichia coli, Salmonella Typhimurium, and Staphylococcus aureus (80.4%, 60.2%, and 65.4%, respectively). These results demonstrate that E. faecalis MD366 can be potentially used as a probiotic with anti-obesity effects.

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Biological Characteristics and Control of Annual Bluegrass (Poa annua) (Annual Bluegrass의 생물학적 특성과 방제)

  • Lee, Sang-Kook
    • Weed & Turfgrass Science
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    • v.2 no.2
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    • pp.122-130
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    • 2013
  • The object of this study is to review the current states of the characteristics and strategies to control annual bluegrass to apply information to the circumstance of South Korea. Annual bluegrass is one of the most widespread turfgrass species which has great ability to produce seedhead and shoot growth. It also has ability to tolerate low mowing height and to form uniformity of turfgrass when it is established. Annual bluegrass is well-known as weak turfgrass for high and low temperature. High rate of nitrogen and phosphorus improves growth of annual bluegrass. To control annual bluegrass, deep and infrequent irrigation is more effective than light and frequent irrigation. Clipping removal is more effective than clipping return to control annual bluegrass. Prodiamine, bensulide, and dithiopyr are applied as pre-emergence herbicide, and ethofumesate and bisbyribac-sodium are used as post-emergence herbicide. Paclobutrazol and flurprimidol are used as plant growth regulator. Trinexapac which is one of the most popular plant growth regulators (PGRs) in South Korea is not proper to control annual bluegrass because it accelerates improve growth of annual bluegrass in summer. Although chemical control is mainly used in South Korea, combination of cultural and chemical control may be the strategy to maximize effectiveness to control annual bluegrass.

Application in Conductive Filler by Low-Temperature Densification and Synthesis of Core-Shell Structure Powder for Prevention from Copper Oxidation (구리 산화 방지를 위한 Core-Shell 구조 입자 합성과 저온 치밀화를 통한 도전성 필러 응용)

  • Shim, Young Ho;Park, Seong-Dae;Kim, Hee Taik
    • Applied Chemistry for Engineering
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    • v.23 no.6
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    • pp.554-560
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    • 2012
  • Recently, it has been increasing trend to use conductive materials as electronics and communication technology in electronics industry are developing. The noble metal such as Ag, Pt, Pd etc. are mostly used as conductive materials, To reduce production cost, alternative materials with similar characteristics of noble metals are needed. Copper has advantages, i.e its electronic properties are similar to noble metals and low cost than noble metal, but its use has been restricted because of oxidation in air. In this study, the tin film was coated on copper by electroless plating to protect copper from oxidation and to confirm the effects of temperature, pH, amount of $SnCl_2$, and feeding speed in plating conditions. Additionally, we apply $Cu_{core}Sn_{shell}$ powder as conductive filler with low-temperature densification and analysis by SEM, XRD, FIB and 4-Point Probe techniques. As result of the study, tin film was coated well on copper and was protected from oxidation. After low-temperature densification treatment, the meted tin made chemical interconnections with copper. Accordingly, conductivity was increased than before condition. We hope $Cu_{core}Sn_{shell}$ powder to replace noble metals and use in the electronic field.

Extraction of Quercetin and Its Glucosides from Onion Edible Part Using Solvent Extraction and Various Extraction Assisting Methods (용매 추출과 병용 추출법을 이용한 양파 과육으로부터 quercetin 관련물질의 추출)

  • Jin, Eun Young;Park, Young-Seo;Jang, Jae Kweon;Chung, Myong-Soo;Park, Hoon;Shim, Kun-Sub;Choi, Young Jin
    • Food Engineering Progress
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    • v.13 no.2
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    • pp.147-153
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    • 2009
  • Quercetin is one of the main flavonoids from onion. To use quercetin as a functional component for onion food products, the effects of various extraction assisting methods such as juicing methods, microwave, ultrasound and enzyme treatments on the yield of quercetin and its glucosides were investigated. For conventional solvent extraction, the highest yield of quercetin and its glycosides was achieved with 0.8 mL/g of 60% methanol at 50$^{\circ}C$ for 15 min. The juicing methods using mixer and screw showed no influence on the yield. Microwave and ultrasound treatments showed 2.14 times and 2.06 times more quercetin yields than non-treated extraction, respectively. For cellulase and viscozyme treatments, the highest yields of quercetin were achieved with 0.5 mL/g of 1% enzyme-0.1M sodium acetate (pH 5.2) buffer solution. Cellulase and viscozyme treatment improved quercetin yield 1.65 times and 2.29 times more than non-treated one, respectively.

Biological Activity in Functional Cosmetic of Purple Sweet Potato Extracts (자색 고구마추출물의 기능성 화장품 활성)

  • Choi, Jae-Hong;Kim, Jin-Sung;Jo, Bun-Sung;Kim, Jeung-Hoan;Park, Hye-Jin;An, Bong-Jeun;Kim, Myung-Uk;Cho, Young-Je
    • Food Science and Preservation
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    • v.18 no.3
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    • pp.414-422
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    • 2011
  • The optimum condition for phenolics contents extraction from purple sweet potato was 12 hrs. in 50% ethanol. The electron donating scavenging activities (DPPH), ABTS radical cation decolorization (ABTS) and antioxidant protection factor (PF) of Jami, Yeonjami and Shinjami were higher than general sweet potato, and thiobarbituric acid reaction substance (TBARs) was below 30%. The minimum inhibitory concentration (MIC) against Staphylococcus aureus and Escherichia coli on skin were each 5,000 and 2,500 ppm in all purple sweet potatoes, and MIC of Jami was the lowest as 2,500 ppm against Staphlococcus epidermidis. The whitening (tyrosinase inhibitory) activity of purple sweet potatoes was the highest as 62.5% and 48.7% in Jami water and ethanol extracts. The anti-inflammation (hyaluronidase inhibitory) activity of purple sweet potatoes was the highest as 25.3% and 94.4% in Jami water and ethanol extracts. The safety of cosmetic with Jam; extracts was assessed by various safety profiles. pH and viscosity change of essence for 90 days was not detected. Cosmetic was stable to temperature and light for 90 days. The result to measure changes of skin color and pore size of the skin was that an aged skin was more effective than young skin.

Cultivation of Paecilomyces tenuipes using Mini-kit, small culture container (소규모 재배상을 이용한 생동충하초 재배)

  • Nam, Sung-Hee;Lee, Kwang Gill;Yeo, Joo Hong;Lee, Heui Sam;Hwang, Jae Sam;Choi, Young-Cheol;Park, Kwan-Ho
    • Journal of Sericultural and Entomological Science
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    • v.50 no.2
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    • pp.116-121
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    • 2012
  • Cordyceps and its allies fungi has been described as a secret medicine that gives eternal youth and a long life. Some species of Cordyceps are sources of biochemicals, such ascordycepin, with interesting biological and pharmacological properties. Hence, it has been studied to uncover its pharmacological effect. We attempted to study the formation of fruiting bodies and to develop means of mass production Korean isolate of Paecilomyces tenuipes has been inoculated into silkworms, where it reproduced using culture container, mini-kit successfully. Culture container, mini-kit is composed of a cylinder-shaped body and lid. The container is made of translucent polyethylene terephthalate. The size of the container is $82{\times}75mm$, reduced by 10 times as compared with the conventional culture kit. The mini kit has many advantages - high culture amount, ability of maintaining optimal humidity, parasite-free cultivation and high-end appearance. With the kit, the optimal cultivation condition is under $22^{\circ}C$, culture period of 53 days. And synnemata of P. tenuipes could be kept fresh for 14 days at the temperature of under $10^{\circ}C$. Therefore, the Min-kit can be used in both ways as a culture container and a packing kit for end-user customers.

Sensor technology for environmental monitoring of shrimp farming (새우양식 환경 모니터링을 위한 센서기술 동향 분석)

  • Hur, Shin;Park, Jung Ho;Choi, Sang Kyu;Lee, Chang Won;Kim, Ju Wan
    • Journal of Sensor Science and Technology
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    • v.30 no.3
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    • pp.154-164
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    • 2021
  • In this study, the IoT sensor technology required for improving the survival rate and high-density productivity of individual shrimp in smart shrimp farming (which involves the usage of recirculating aquaculture systems and biofloc technology) was analyzed. The principles and performances of domestic and overseas water quality monitoring IoT sensors were compared. Furthermore, the drawbacks of existing aquaculture monitoring technologies and the countermeasures for future aquaculture monitoring technologies were examined. In particular, for farming white-legged shrimp, an IoT sensor was employed to collect measurement indicators for managing the water quality environment in real-time, and the IoT sensor-based real-time monitoring technology was then analyzed for implementing the optimal farming environment. The results obtained from this study can potentially contribute to the realization of an autonomous farming platform that can improve the survival rate and productivity of shrimp, achieve feed reduction, improve the water quality environment, and save energy.

Impurity analysis and acid leaching purification of silica minerals (실리카광물의 산침출 정제와 불순물 분석법 연구)

  • Lee, Kil Yong;Yoon, Yoon Yeol;Cho, Soo Young;Chae, Young-Bae
    • Analytical Science and Technology
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    • v.20 no.6
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    • pp.516-523
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    • 2007
  • Purification of silica mineral has been investigated by acid leaching of pulverized silica. A series of studies has been carried out on the effect of leaching silica powder as a function of the leaching time at the constant temperature of $80^{\circ}C$ in oxalic acid, aqua regia, and two mixed acids of HF/HCl, $HF/HNO_3$. The impurities of silica and leachantes were measured by neutron activation analysis (NAA), inductively coupled plasma atomic emission spectrometry (ICP-AES), atomic absorption spectrometry, x-ray fluorescence (XRF) method and wet analysis (WA). Certain metals, such as sodium, calcium, iron, aluminium and titanium, have been found in concentrations of hundreds or even thousands of mg/kg. Comparison of purification processes of silica and analytical methods of impurities in the silica was conducted in this study.

Production of Dendropanax morbiferus extract containing multi-functional ingredients by serial fermentation using Bacillus subtilis HA and Lactobacillus plantarum KS2020 (고초균-젖산균의 순차적 복합 발효를 통한 복합 기능성 물질 함유 황칠나무 추출물의 생산)

  • Su-Jin Son;Hye-Mi Kang;Yun-Ho Park;Mi-Hyang Hwangbo;Sam-Pin Lee
    • Food Science and Preservation
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    • v.31 no.1
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    • pp.138-148
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    • 2024
  • The production of poly-γ-glutamic acid (γ-PGA) and γ-aminobutyric acid (GABA) was optimized by serial fermentation of Dendropanax morbiferus extract (DME) using Bacillus subtilis HA and Lactobacillus plantarum KS2020. The 1st alkaline fermentation was performed on 60% DME including 2% glucose and 10% monosodium ʟ-glutamate (MSG) as a precursor. The 1st fermented DME had 57 mg% tyrosine. Consequently, the 2nd lactic acid fermentation for 5 days increased the tyrosine content of 106 mg%. The mucilage containing γ-PGA showed a high content of 3.50% on the first day of alkaline fermentation and then increased to 4.10% after 2 days. The precursor (MSG) remaining in the 1st fermented DME was efficiently converted to GABA by the 2nd lactic acid fermentation in the presence of 5% skim milk, 1.5% glucose and 0.5% yeast extract, resulting in the production of 18.29 mg/mL GABA. The viable cells of lactic acid bacteria increased and indicated 9.49 log CFU/mL on the fermentation for 5 days, and the acidity of co-fermented DME indicated the highest value of 1.55%. Conclusively, the serial fermented DME has multi-functional ingredients containing γ-PGA, GABA, peptides and probiotics.

Changes of Major Components and Microorganisms during the Fermentation of Korean Ordinary Kochujang (한국재래식(韓國在來式)고추장숙성중(熟成中)의 주요성분(主要成分) 및 미생물(微生物)의 변화(變化))

  • Ahn, Cheol-Woo;Sung, Nack-Kie
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.16 no.3
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    • pp.35-39
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    • 1987
  • The present study was attempted to obtain the basic data concerning a reasonable preparing method and the optimum fermentation conditions of Kochujang (Red pepper paste). To establish the standard qualify of Kochujang, changes of the chemical composition and the numbers of bacteria and feasts in Kochujang during fermentation were observed. Moisture, salts and crude ash contents of Kochujang were not changed significantly during fermentation. Titrable acidity and amino nitrogen gradually increased with the time-passed, whereas crude fat gradually decreased with the time-elapsed. And reducing sugar and total nitrogen increased until 40 days, but slightly decreased after this period. The numbers of bacteria and yeasts in the ingrients for the preparation of Kochujang were $3.9{\times}10^7/g$, $1.5{\times}10^3/g$ in red pepper powder, $7.6{\times}10^4/g$, $2.8{\times}10^2/g$ in salts. respectively, but those of sugar and malt were not more than 100/g. Microbial counts in Kochujang during fermentation increased until 40 days, but those are gradually decreased after that.

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