• KSBB Journal
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• v.5 no.3
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• pp.279-291
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• 1990

Partial hydrolysed and deacetylated chitin, CHITA and CHITB as supports of immobilized enzyme were obtained by treatment of acid and base respectively. Glutaraldehyde, bifunctional reagent, was employed for crosslinking between $\beta$-glucosidase and support. Immobilized enzyme activities of CHITA-Gase and CHITB-Gase were determined with the reaction of p-nitrophenol-$\beta$-D-glucopyranoside(PNG) in batch reactor, CSTR and PFR. Their optimum temperature, pH and enzymatic characteristics including Km and Vmax values were observed with variation of the flow rates. Mass transfer coefficient(h), effectiveness factor(η), deactivation rate(kd ) of two immobilized enzymes were also examined to compare efficiency of reactors.

• Microbiology and Biotechnology Letters
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• v.15 no.6
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• pp.375-380
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• 1987

D-$\alpha$-Amino-$\varepsilon$-carpolactam racemase (EC 5.1.1) and L-$\alpha$-amino-$\varepsilon$-caprolactam hydrolase (EC 3.5.2) were fractionated from cell-free extracts of Alcaligenes eutrophus A52 using ammonium sulfate precipitation and DEAE-cellulose ion exchange chromatography. It was made sure that D-$\alpha$-amino-$\varepsilon$-caprolactam was converted to L-$\alpha$-amino-$\varepsilon$-caprolactam by racemase, and then hydrolyzed into L-lysine by hydrolase in Alcaligenes eutrophus A52. For the conversion of D-$\alpha$-amino-$\varepsilon$-caprolactam into L-lysine by cell-free extracts of Alcaligenes eutrophus A52, the optimum temperature and pH were 6$0^{\circ}C$ and 8.5 respectively. The results showed that 0.5% D-$\alpha$-amino-$\varepsilon$-caprolactam was converted to L-lysine at 55$^{\circ}C$ for 10 hr with a conversion rate of 98% by cell-free extracts containing 3.1mg of protein.

• Journal of the Korean Applied Science and Technology
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• v.33 no.1
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• pp.176-185
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• 2016

Yeonsan Ogae has been known as supporting health and high efficacy of treatment. In recent days, as the efficacy of functional peptides has known, the optimization of oligo peptides production and its characteristics from Ogae legs has been performed. Response surface method was used to perform the optimizaion of enzyme hydrolysis. The range of processes was temperature ( 40, 50 and $60^{\circ}C$), pH( pH 6.0, 7.0 and 8.0 ), and enzyme( 1, 2 and 3% ). The degree of hydrolysis, amino acids, molecular weight of products were analyzed. The optimum process of enzyme hydrolysis were determined as temperature $58^{\circ}C$, pH 7.5, and enzyme concentration 3%. At optimum conditions, the degree of hydrolysis after 2 h reaction was 75-80%. The amino acid and were 168.131 mg/100 g, respectively. The molecular weight of products by using MALDI-TOF was ranged from 300 to 1,000 Da.

• Journal of Aerospace System Engineering
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• v.18 no.2
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• pp.71-78
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• 2024

During the initial thermal design process, determining the thermal effect of various design variables in a complex orbital thermal environment is time-consuming. To save time in the initial design phase, it is necessary to quickly derive optimal design parameters and predict the temperature. To address these challenges, Veritrek, a software specialized in optimal design using a reduced-order model (ROM), was released in 2018. In this paper, we utilized the Veritrek software to build a reduced-order model, conduct sensitivity analysis, and perform optimal design analysis for a graphite sheet-based cryogenic cooler thermal control system. The goal was to determine the optimal design values for the number of graphite sheet layers, radiator area, and thickness that would meet the allowable temperature of the cryogenic cooler.

• Proceedings of the KIEE Conference
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• 2015.07a
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• pp.418-419
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• 2015

지중케이블이 전력구에 포설되어 운영되는 경우 케이블 부하에 따른 발열로 전력구 내부온도가 상승하게 된다. 이 때 전력구 내부온도가 $40[^{\circ}C]$를 초과하는 경우, 케이블의 상시 허용전류의 크기가 설계 값보다 작아짐으로써 케이블 송전용량이 감소하는 문제점이 발생한다. 또한 전력구 내부온도는 산업안전보건기준에서 $37[^{\circ}C]$ 이하로 유지할 것을 권고하고 있어 안정적인 전력공급을 위한 대책 수립 및 최적의 운영을 위하여 전력구 내부 케이블의 부하전류 변동에 따른 온도변화 추이 예측이 요구된다. 따라서 본 논문에서는 수도권에 설치되어 운영 중인 지중케이블의 부하변동에 따른 전력구 내부온도 변화 특성을 검토하고자 한다.

• Proceedings of the KAIS Fall Conference
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• 2010.05b
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• pp.996-999
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• 2010

고압의 가스를 이용하여 고온 가스와 저온 가스를 분리하거나 입자상 물질의 분리에 사용할 수 있는 장치인 볼텍스 튜브의 에너지 분리 특성을 파악하기 위하여 $100Nm^3$/hr급 볼텍스 튜브를 제작하고 이에 대한 실험을 진행하였다. 저온측의 유량비와 오리피스 직경 및 볼텍스 튜브의 길이가 온도에 미치는 영향을 분석하였다. 오리피스 직경 0.6D에서 최적의 온도 분리 효과를 나타내었으며, 0.8D의 경우 그 효과가 미미하였다. 또 한 오리피스 직경이나 길이에 관계없이 저온유량비가 약 0.9부근에서 고온측의 온도가 최고점을 나타내었고, 볼텍스 튜브 길이는 저온측 온도 변화에 미미한 영향을 미치나, 오리피스 직경의 변화는 최저 온도점이 나타나는 저온 유량비에 상당한 영향을 미쳤다. 본 연구의 결과는 $100Nm^3$/hr급 볼텍스 튜브의 최적화를 위한 기초자료로 활용될 수 있을 것이다.

• Korean Journal of Food Science and Technology
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• v.29 no.2
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• pp.343-347
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• 1997

Effect of culture conditions on the fermentation of wheat flour solution by mixed lactic acid bacteria of Lactobacillus brevis, L. fermentum and L. plantarum was investigated. The optimum temperature for the fermentation of wheat flour solution was $35^{\circ}C$ because pH decreased the lowest value and TTA (total titrable acidity) increased the highest value at this temperature. In aerobic condition, fermentor was purged with air at 1.0 vvm and was purged with nitrogen gas at 1.0 vvm in anaerobic condition. The decrease of pH and the increase of TTA in aerobic condition were higher than those in anaerobic condition. In aerobic condition, the optimum condition of oxygen supply was found to be oxygen transfer rate coefficient of $60\;hr^{-1}$ which corresponded to agitation speed of 250 rpm in a 5 L fermentor. Repeated fed-batch cultures were performed using pH-stat in order to increase the productivity of fermented wheat flour. With increasing the repeated fraction of culture volume, mean cycle time increased but maximum operation time decreased. However, the volume of produced broth per culture volume per time and total volume of produced broth per culture volume were maximum at the repeated fraction of culture volume of 20%. In a repeated fed-batch fermentation of wheat flour solution using mixed lactic acid bacteria, the culture condition was optimum at temerature of $35^{\circ}C$, aeration rate of 1.0 vvm, oxygen transfer rate coefficient of $60\;hr^{-1}$, and repeated fraction of culture volume of 20%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.2
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• pp.228-233
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• 2001

This study was carried out to characterize the enzymatic properties and effectiveness on the firmness of pectinesterase(PE) and polygalacturonase(PG) which are present in carrot cell wall, and to determine the blanching condition of raw carrot. Crude enzyme was extracted from carrots and used as PE and PG. The optimum pH of PE and PG activity were 7.0 and 5.0, respectively. NaCl enhanced PE and PG activities, particularly at 0.15 M and 0.10 M, respectively. Although the optimum temperature of PG ($70^{\circ}C$) was higher than that of PE ($50^{\circ}C$), PE ($Z-value=8.76^{\circ}C$) was more heat-stable than PG($Z-value=6.67^{\circ}C$). Blanching condition was determined as at $55^{\circ}C$ for 60 min in 0.03 M $CaCl_2$, 0.1 M NaCl and pH 7.0 from measuring firmness after blanching at various temperature ($50~70^{\circ}C$) and time (5~60 min).

• The Korean Journal of Mycology
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• v.9 no.1
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• pp.31-37
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• 1981

Mycelial growth and fruit body formation of Diehlomyces microsporus were best on mushroom spawn extract medium and rice bran extract medium, respectively. L-asparagine, fructose and glucose were good nutrient sources for mycelial growth. Optimum temperature for mycelial growth ranged at $25{\sim}28^{\circ}C$. Maximum mycelial growth occurred at pH 5.5 while optimum pH for ascospore germination was 6.0. Mycelial mats of D. microsporus did not survive at $60^{\circ}C$ for 60 minutes while ascospores at $80^{\circ}C$ for 120 minutes. Damages of fruit body of Agaricus bisporus caused by D. microsporus were maximum when the fruit bodies were infected at spawning and casing on the compost. The truffle disease could be controlled by basamid with $100{\sim}150 ppm$ treating on the compost after filling.

• Proceedings of the Korea Water Resources Association Conference
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• 2006.05a
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• pp.1400-1404
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• 2006

생물학적 질소, 인 제거 공정(이하 BNR)의 운전에 있어서 최적 유입수의 C/N(COD/TKN)비, SRT 및 온도의 범위 및 정량적 수치 등은 유기물 뿐 만아니라 질소, 인의 처리 효율에 있어서 매우 중요하다. 특히, 외국과 다른 저농도 유기물 특성을 보이는 국내 하수에 대해서는 BNR 공정의 선택과 설계 및 운전인자의 선별이 무엇보다도 중요한 역할을 한다. 본 연구에서는 IAWQ에서 제시한 ASM No.2d를 기초로 하여 만들어진 전산모형인 Envirosim사의 Biowin 프로그램을 시뮬레이션 도구로 활용하여, 국내 하수에 비교적 적용하기 용이한 A2/O 공정과 MUCT 공정에 대한 유기물, 질소 및 인처리 효율을 비교하고 유입수의 C/N와 SRT 및 온도에 따른 질소, 인 처리 특성과 유출수의 거동 등을 파악하였다. 시뮬레이션 결과, 국내 하수에서는 A2/O 보다는 MUCT 공정이 질소, 인 처리효율이 더 크게 나타났다. 온도와 SRT가 일정한 상태에서 C/N비는 7이상에서 TKN과 TP제거효율이 양호하게 나타났고, 온도와 C/N비를 일정한 조건에서는 SRT가 7일을 넘어서면 효율이 급격히 낮아지는 현상을 관찰할 수 있었다. 온도조건 실험에서는 $20^{\circ}C$이하, 특히 국내 하수처리장에 BNR 적용시 설게조건인 $13^{\circ}C$에 근접해서는 TKN의 제거효율은 급격히 떨어지는 반면에 인 제거효율이 상승하는 것으로 나타났다.