• Journal of Life Science
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• v.16 no.6
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• pp.1001-1005
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• 2006

The objective of this study was carried out to evaluate the suitability of simple sequence repeat (SSR) markers for genetic diversity assessment and identification of rice varieties. The 23 primers selected from 50 SSR primers showed polymorphisms in the 21 rice varieties. The 2 to 9 SSR alleles were detected for each locus with an average of 3.00 alleles per locus. The polymorphism information content (PIC) ranged form 0.091 to 0.839. Based on band patterns, UPGMA cluster analysis was conducted. These varieties were separate into 4 groups corresponding to rice ecotype and pedigree information and genetic distance of cluster ranging from 0.59 to 0.92. The 4 SSR primer sets (RM206, RM225, RM418, RM478) selected form 23 polymorphic primers were differentiated all the rice variety from each other by markers genotypes. These markers may be used wide range of practical application in variety identification of rice.

• Journal of KIISE:Information Networking
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• v.28 no.2
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• pp.277-287
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• 2001

Test derivation methods suitable for interoperability testing of communication protocols were proposed in [1,2, 3] and applied to the TCP and the ATM protocols, The test cases that were generated by them deal with only the control part of the protocols. However, in real protocol testing, the test cases must manage the data part as well. For complete testing, in principle we must test all possible values of data part although it is impractical to do so. In this paper, we present a method generating the interoperability test suite for both the data part and the control part of protocols with the example of Tep connection establishment. In this process, we make use of experimental design techniques from industrial engineering to minimize the size of test suite while keeping testing capability. Experimental design techniques have been used for protocol confom1ance testing but not for intcruperability testing so far. We generate the test suite for data part by this method and show a possibility that we can test interoperability of protocols with the minimum number of test cases while maintaining the testing power.

• Journal of Food Hygiene and Safety
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• v.35 no.1
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• pp.51-59
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• 2020

This study evalutated the risk of foodborne illness from Vibrio spp. (Vibrio vulnificus and Vibrio cholerae) through sea squirt consumption. The prevalence of V. vulnificus and V. cholerae in sea squirt was evaluated, and the predictive models to describe the kinetic behavior of the Vibrio in sea squirt were developed. Distribution temperatures and times were collected, and they were fitted to probabilistic distributions to determine the appropriate distributions. The raw data from the Korea National Health and Nutrition Examination Survey 2016 were used to estimate the consumption rates and amount of sea squirt. In the hazard characterization, the Beta-Poisson model for V. vulnificus and V. cholerae infection was used. With the collected data, a simulation model was prepared and it was run with @RISK to estimate probabilities of foodborne illness by pathogenic Vibrio spp. through sea squirt consumption. Among 101 sea squirt samples, there were no V. vulnificus positive samples, but V. cholerae was detected in one sample. The developed predictive models described the fates of Vibrio spp. in sea squirt during distribution and storage, appropriately shown as 0.815-0.907 of R² and 0.28 of RMSE. The consumption rate of sea squirt was 0.26%, and the daily consumption amount was 68.84 g per person. The Beta-Poisson model [P=1-(1+Dose/β)^-α] was selected as a dose-response model. With these data, a simulation model was developed, and the risks of V. vulnificus and V. cholerae foodborne illness from sea squirt consumption were 2.66×10^-15, and 1.02×10^-12, respectively. These results suggest that the risk of pathogenic Vibrio spp. in sea squirt could be considered low in Korea.

• Korean journal of applied entomology
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• v.29 no.3
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• pp.209-215
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• 1990

Some preliminary studies were conducted to find out whether the levee-burning could justifiable for the suppression of insect pests, particularly the smaller brown planthopper (Laodelphax striatellus F.). Density surveys on pests and their enemies (mostly spiders) were carried out upto the mid May at an experimental paddy field located in Suwon after of it's levee $(72\times1m)$ was burned on Feb. 20, 1987. Results were discussed in relation to density recovering of both pests and their possible enemies (spiders) and summarized as below. Not a single individual of any pest or enemy was found from the levee upto sometime after the levee-burning. Grasses started to grow more vigorously in burned ares than in unburned upto about 60 days after the burning. And densities of both pest and enemies grew higher in burned areas than in unburned from about 75 days after the burning (in Early may). It is suspected that all individuals of pests and enemies fond from the burned areas could have immigrated from the surrounding areas. If levee-burning was carried out in much wider areas, much longer time would be needed to recover the density of both pests and enemies to the center region of the burning. Wingless spiders would require even longer time than winged pest species to re-establish in the center region of the widely burned field. Pirata subpiraticus, the most abundant spider species in Korean paddy fields, starts to move about and searches for food at above $9^{\circ}C$ which is somewhat lower than the critical temperature for the pest species. Thus P. subpiraticus would require more food than other pest species early in the spring, and therefore, it would have lower probability to survive than pest species particularly in burned areas. Experiments for pest suppression with levee-burning would better be carried on in much wider areas, and its justification seems to be discussed after man other disciplines related to both pests and their natural enemies were throughly studied together with their density surveys. However, according to the present point of vie, the opinion that levee-burning is helpful for controlling pests which over winter on levee areas could not be justifiable.

• Food Science and Preservation
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• v.24 no.1
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• pp.27-35
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• 2017

Microbiological, physicochemical, and organoleptic properties of fresh-cut vegetables (FVs), carrots, green peppers, cherry tomatoes, and paprika after X-ray irradiation were evaluated to verify food quality suitable for the immune-depressed patients. Total concentrations of aerobic bacteria in non-irradiated samples, except for cherry tomatoes, were 1.63-3.34 log CFU/g. Irradiation dose exceed 0.4 kGy was used for carrots and green peppers whereas the sterilization dose of 0.2 kGy was used for both cherry tomatoes and paprika. A dose of 0.4 kGy was tentatively determined as the minimum allowable dose for sterilization of the FVs, based on $D_{10}$-values of X-ray irradiation (0.11-0.32 kGy) for Escherichia coli, Listeria monocytogenes, and Salmonella Typhimurium inoculated on the samples. With respect to the physiological properties, only hardness was significantly decreased as the absorbed dose increased; however, there were no significant differences in hardness of the sterilized samples using X-rays at 0.4 kGy compared with those of non-irradiated samples (p<0.05). Moreover, overall acceptance scores of the sterilized FVs were higher than 5.0 points on a 7-point scale, indicating a good organoleptic quality. In a survey on preference of hospitalized patients with cancer (n=50), the average scores for the sterilized FVs, except for carrots, were higher than 4.0 points. In conclusion, it is considered that the FVs, except for carrots, sterilized using X-rays at 0.4 kGy could be served to immune-depressed patients as hygienically safe foods with acceptable organoleptic properties.