• Title/Summary/Keyword: 총 소화관

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Studies on the Food Organisms of Bivalve, Mactra veneriformis in Shore of Kunsan (군산연안 동죽의 먹이생물에 대한 연구)

  • Ryou Dong-Ki;Kim Yong-Ho
    • Journal of Aquaculture
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    • v.8 no.2
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    • pp.99-115
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    • 1995
  • The food organisms of bivalve, Mactra veneriformis Reeve were investigated, from August 1993 to July 1994, at an intertidal sandflat area in shore of Kunsan. In the digestive track of M. veneriformis, total 60 genera (120 species) were found and consisted of both 52 phytoplankton genera (31 Diatoms, 7 Dinophyceae, 6 Chrysophyceae, 5 Cyanophyceae, and 3 Chlorophyceae) and 8 zooplankton genera (4 Radiolaria, 2 Ciliata, and 2 Crustaceae). The number of planktons species from digestive track readed the peak in March and October, and that in August is the lowest. The dominant planktons of the environment and the digestive track of M veneriformis are not coincidence. These planktons were roughly divided into two tropes as round and bar. The former was less than $200{\mu}m$ in diameter and the latter less than $20{\mu}m$ in diameter and $500{\mu}m$ in length.

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Studies on the Feeding Activity and Environmental Tolerance of Geoduck Clam, Panope japonica (코끼리조개의 섭식활동 환경내성에 관한 연구)

  • 이채성
    • Journal of Aquaculture
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    • v.10 no.2
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    • pp.213-218
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    • 1997
  • Experiments on the feeding activity and environmental tolerance of geoduck clam, Panope japonica caught at the coastal water of Kangwon province located in the East Sea of Korea were performed from January to December, 1994. Twenty genera and 33 species of plankton were found in the digestive tract, and most of them are phytoplankton. In spring, 21 species of planktonic foods were indentified, but in sumer, only 11 species were identified. Nitzschia longissima and Rizosolenia alata were present evey month. Weight of the digestive tract was fluctuate seasonally : higher winter and spring, and lower summer. Adult geoduck clams could be tolerated within the range of 5 to $20^{\circ}C$ water temperature, and they could tolerated considerably well temperature than higher temperature within the range.

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Effects of Cecal Ligation and Colostomy on Food and Water Intake and loafer Excretion in Chickens Fed Restrictedly and Freely (닭에 있어서 사료섭취의 자유 및 제한급여시킬 때의 사료섭취량, 음수량 및 수분 배설량에 미치는 맹장결찰 및 인공항문 수술의 효과)

  • Son, J.H.
    • Korean Journal of Poultry Science
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    • v.29 no.4
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    • pp.259-263
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    • 2002
  • It was examined whether the ceca and the back-flow of urine into the lower intestine are involved in water intake and excretion in chickens and food intake affects those. Colostomy significantly increased water intake, total water excretion and the ratio of the water intake to food intake in the ceca-ligated chickens under restrict and ad libitum feeding conditions (P<0.05), but the increases were much larger in chickens fed ad libitum than in those fed restrictedly. Cecal ligation increased water intake, total water excretion and the ratio of water intake to food intake in the colostomised chickens which were fed freely (P<0.05). but not in those fed restrictedly, None of colostomy and cecal ligation affected the resultant water balances in chickens under both feeding conditions. Colostomy increased food intake in the ceca-ligated chickens (P<0.05), but no increase by cecal ligation was observed in colostomised chickens. It is concluded that the lower intestine takes a very important role in water recovery from urine to maintain water balance in chickens.

Survey of Expressed Sequence Tags from Tissue-Specific cDNA Libraries in Hemibarbus mylodon, an Endangered Fish Species (멸종위기 어류 어름치 Hemibarbus mylodon (Cypriniformes)로부터 조직별 EST library 제작 및 발현 유전자 탐색)

  • Bang, In-Chul;Lim, Yoon-Hee;Cho, Young-Sun;Lee, Sang-Yoon;Nam, Yoon-Kwon
    • Journal of Aquaculture
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    • v.20 no.4
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    • pp.248-254
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    • 2007
  • Representative cDNA libraries were constructed from various tissue sources of Hemibarbus mylodon, an endangered freshwater fish species in Korea, for the mining of expressed sequence tags (ESTs). Randomized and non-normalized EST analysis was performed with 7 unidirectional cDNA libraries generated from brain, intestine, kidney, liver, muscle, ovary or testis. Of 3,383 ESTs in total, the number of singleton was 2,029, and 333 contigs containing 1,354 ESTs were assembled (percent of unigene = 70.0%). Abundantly expressed gene transcripts and broad clustering of putative gene function were tissue-specific in general, and the redundancy was also variable among those libraries. Over half of H. mylodon ESTs were matched with orthologues from other teleosts among which zebrafish gene sequences were the most frequent in those matches. This initial setting of EST libraries achieved in the present study would be a fundamental basis for the banking of gene resources from this endangered fish species.

Identification of Enteric Bacteria from Nephila clavata (한국산 무당거미(Nephila clavata)에서 분리한 장내 세균의 동정)

  • 문은영;오현우;맹필재;배경숙
    • Korean Journal of Microbiology
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    • v.37 no.1
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    • pp.1-8
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    • 2001
  • Spiders are carnivores that prey upon insects and other small arthropods through digestion of food outside the body. Although spider poison may contain proteolytic enzymes, these are thought to play an insignificant role in actual digestion. The source of active proteolytic enzymes can be either the digestive tract cells of spider, or natural microbial flora in the digestive tract of spider. In this study, digestive tracts from the spider, Nephila clavata, were screened for bacteria that have protease or lipase activity. A total of $10^3-10^5$ CFU was recovered from a spider and more than 90% of them showed protease and lipase activity respectively. Of the microbial isolates, 63.3% showed protease or lipase activity, and 50% of these showed both protease and lipase activity. Some of the isolates were characterized using a battery of chemical, phenotypic and genotypic methods. Eleven Gram negative bacteriaa (Acinetobacter calcoaceticus, A. haemolyticus, Alcaligenes faecalis, Cedecea davisae, C. neteri, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas fluorescens, Serratia marcescens, Stenotrophomonas maltophilia, Suttonella indologenes) and 11 Gram positive bacteria (Bacillus cereus, B. coagulans, B. pasteurii, B. thuringiensis, Cellulomonas flavigena, Corynebacterium martruchotii, Enterococcus durans, E. faecalis, Micrococcus luteus, Staphylococcus hominis, S. sciuri) were identified.

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Esterase Isozymes of Beet Armyworm, Spodoptera exigua(H bner), with Development and Tissues (발육 및 조직에 따른 파밤나방(Spodoptera exigua (H bner)) 에스테라제 동위효소)

  • 강성영;김용균
    • Korean journal of applied entomology
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    • v.37 no.2
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    • pp.179-185
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    • 1998
  • The effect of physiological factors of beet armyworm, Spodoptera exigua (Hubner), on esterase variation was analyzed by comparing electrophoretic esterase isozymes. Each esterase isozyme was also characterized by substrate and inhibitor specificities. A total of 28 esterase isozymes were separated on 10% nondenaturing polycarylamide gel electrophoresis (PAGE). These isozymes were denoted from El to E28 according to cathodal migration distances. There was a variation in esterase isozymes among developmental stages. Larvae and pupae had more isozymes than did adults. Eggs had only eight isozymes. The isozymes of El and E2 were specific only in the first instar larvae. Esterases also showed variation according to different tissues. More kinds of esterase isozymes were found in epidermis and gut tissues than in hemolymph and fat body. Some isozymes were specific in epidermis (from El to E6), gut (E10, El 1, E25, E26, and E27), and hemolymph (E18). Among 10 naphthyl esters, a-naphthyl propionate was the most reactive substrate to the esterase isozymes. The isozymes were classified into cholinesterases (El0 and E24), arylesterases (E4, E9, E17, E19, E21, and E23), and carboxylesterases (the others) on the basis of inhibition by the esterase inhibitors-eserine, dichlorovos, moncrotophos, and paraoxon.

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Studies on Photosensitized Oxidation in the Lipids of Irish moss, Laver and Oyster (진두발, 김 및 굴의 지질에 있어서 광증감 산화에 관한 연구)

  • KIM Kui-Shik;KOIZUMI Chiaki;BAE Tae-Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.3
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    • pp.355-360
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    • 1997
  • In order to investigate the influence of photosensitized oxidation in the sun-dried irish moss (Chondrus Ocellatus), laver (Porphyra Yezoensis) and ultra violet irradiated oyster (Crassostrea gigas) the oxidation of lipid and isomers of hydroperoxides were analyzed by gas chromatography-mass spectrometry. The lipid contents of oyster, irish moss and layer were $2.7\%,\;0.1\%,\;0.1\%$ of respectively. Peroxide value, 56,7 meq/kg in the raw oyster was increased of 100.9 meq/kg by the U.V, irradiation for 4 hours. Also the peroxide values of the irish moss and laver were increased by the sun-drying. In the identification of hydroperoxides isomers by trimethylsily (TMS) derivative of photo-oxidized lipid from oyster, irish moss and laver, the proportions of positional isomer, 9-OOH and 13-OOH were dominant than those 10-OOH and 12-OOH.

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Molecular Characterization and Expression Analysis of a Glutathione S-Transferase cDNA from Abalone (Haliotis discus hannai) (북방전복 (Haliotis discus hannai)에서 분리한 Glutathione S-transferase 유전자의 분자생물학적 고찰 및 발현분석)

  • Moon, Ji Young;Park, Eun Hee;Kong, Hee Jeong;Kim, Dong-Gyun;Kim, Young-Ok;Kim, Woo-Jin;An, Cheul Min;Nam, Bo-Hye
    • The Korean Journal of Malacology
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    • v.30 no.4
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    • pp.399-408
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    • 2014
  • Glutathione S-transferases (GSTs) are a superfamily of detoxification enzymes that primarily catalyze the nucleophilic addition of reduced glutathione to both endogenous and exogenous electrophiles. In this study, we isolated and characterized a full-length of alpha class GST cDNA from the abalone (Haliotis discus hannai). The abalone GST cDNA encodes a 223-amino acid polypeptide with a calculated molecular mass of 25.8 kDa and isoelectric point of 5.69. Multiple alignments and phylogenetic analysis with the deduced abalone GST protein revealed that it belongs to the alpha class GSTs and showed strong homology with disk abalone (Haliotis discus discus) putative alpha class GST. Abalone GST mRNA was ubiquitously detected in all tested tissues. GST mRNA expression was comparatively high in the mantle, gill, liver, and digestive duct, however, lowest in the hemocytes. Expression level of abalone GST mRNA in the mantle, gill, liver, and digestive duct was 182.7-fold, 114.8-fold, 4675.8-fold, 406.1-fold higher than in the hemocytes, respectively. Expression level of abalone GST mRNA in the liver was peaked at 6 h post-infection with Vibrio parahemolyticus and decreased at 12 h post-infection. While the expression level of abalone GST mRNA in the hemocytes was drastically increased at 3 h post-infection with Vibrio parahemolyticus. These results suggest that abalone GST is conserved through evolution and may play roles similar to its mammalian counterparts.

Molecular Characterization and Expression Analysis of Peroxiredoxin 2 cDNA from Abalone (Haliotis discus hannai) (참전복(Haliotis discus hannai)에서 분리한 peroxiredoxin 2 유전자의 분자생물학적 고찰 및 발현분석)

  • Moon, Ji Young;Park, Eun Hee;Kong, Hee Jeong;Kim, Young-Ok;Kim, Dong-Gyun;An, Cheul Min;Nam, Bo-Hye
    • Journal of Life Science
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    • v.24 no.12
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    • pp.1291-1300
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    • 2014
  • Peroxiredoxins (Prxs) are a ubiquitous family of antioxidant enzymes that participate in a variety of biological processes, including $H_2O_2$-mediated signal transduction, molecular chaperoning, and mitochondrial function. In this study, we isolated and characterized a Prx 2 cDNA from abalone (Haliotis discus hannai). The abalone Prx 2 cDNA encoded a 199-amino acid polypeptide that belongs to a class of typical 2-Cys Prxs that contain peroxidatic and resolving cysteines. The deduced abalone Prx 2 protein showed strong homology (64-99%) with Prx 2 proteins from other species, including mollusk, fish, amphibians, and mammals, and it was most closely related to disk abalone (H. discus discus) Prx 2. Abalone Prx 2 mRNA was ubiquitously detected in tested tissues, and its expression was comparatively high in the mantle, gills, liver, foot, and digestive duct. The expression level of abalone Prx 2 mRNA was 106.7-fold, 51.9-fold, and 437.8-fold higher, respectively, in the gills, digestive duct, and liver than in the muscles. The expression level of abalone Prx 2 mRNA in the liver peaked at 6 hr postinfection with Vibrio parahemolyticus and decreased at 12 hr postinfection. The expression level of abalone Prx 2 mRNA in hemocytes was drastically increased at 1 hr postinfection with V. parahemolyticus. These results suggest that abalone Prx 2 is conserved through evolution and that it may play a role similar to that of its mammalian counterpart.

Comparison of the Chicken Large Intestine to the Large Intestine of Pigs and the Rumen of Cows (닭의 대장을 돼지의 대장이나 소의 제 1위 (Rumen)와 비교 연구)

  • Nahm K.H.
    • Korean Journal of Poultry Science
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    • v.33 no.1
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    • pp.81-95
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    • 2006
  • The large intestine of the chicken differs both anatomically and physiologically from the pig's large intestine and the men of the cow. The chicken's large intestine is less developed than the pig's large intestine or the cow's lumen. This paper summaries these differences. The chicken's large intestine contains a microbiological population similar to that found in the rumen. The chicken's caeca especially contains a large number of microorganisms, but this population varies according to age, fred, maturity, antibiotic use and etc.. Protein is an essential nutrient for the formation of intestinal microvilli. A study showed that the length of the small intestine was 63 % of the total gastrointestinal tract (GIT) length, while caecum was 8.1 %, and the colon and rectum were 4.6 %. The establishment of the microbial population of the small intestine occurs earlier than that of the caeca, but the identity of approximately 90 % of microbial population of the chicken GIT is hon. Recent studies have shown that energy, volatile fatty acid (VFA) and electrolytes that are found in the large intestine may be absorbed to a certain degree. The chicken small intestine is the primary location for digestion with a variety of enzymes being secreted here. Much research is being conducted into the digestion of sucrose thermal oligosaccharide caramel (STOP), fructooligosaccharides (FOS), mannanoligosaccharide (MOS), galactooligosaccharides (GOS) and isomalto-oligosaccharides (IMO) in the chicken caeca and large intestine. Excessive fibre content in the feed has detrimental effects, but proper fibre supplementation to chicken diets can improve the length and capacity of the small intestine.