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http://dx.doi.org/10.5352/JLS.2014.24.12.1291

Molecular Characterization and Expression Analysis of Peroxiredoxin 2 cDNA from Abalone (Haliotis discus hannai)  

Moon, Ji Young (Biotechnology Research Division, NFRDI)
Park, Eun Hee (Biotechnology Research Division, NFRDI)
Kong, Hee Jeong (Biotechnology Research Division, NFRDI)
Kim, Young-Ok (Biotechnology Research Division, NFRDI)
Kim, Dong-Gyun (Biotechnology Research Division, NFRDI)
An, Cheul Min (Biotechnology Research Division, NFRDI)
Nam, Bo-Hye (Biotechnology Research Division, NFRDI)
Publication Information
Journal of Life Science / v.24, no.12, 2014 , pp. 1291-1300 More about this Journal
Abstract
Peroxiredoxins (Prxs) are a ubiquitous family of antioxidant enzymes that participate in a variety of biological processes, including $H_2O_2$-mediated signal transduction, molecular chaperoning, and mitochondrial function. In this study, we isolated and characterized a Prx 2 cDNA from abalone (Haliotis discus hannai). The abalone Prx 2 cDNA encoded a 199-amino acid polypeptide that belongs to a class of typical 2-Cys Prxs that contain peroxidatic and resolving cysteines. The deduced abalone Prx 2 protein showed strong homology (64-99%) with Prx 2 proteins from other species, including mollusk, fish, amphibians, and mammals, and it was most closely related to disk abalone (H. discus discus) Prx 2. Abalone Prx 2 mRNA was ubiquitously detected in tested tissues, and its expression was comparatively high in the mantle, gills, liver, foot, and digestive duct. The expression level of abalone Prx 2 mRNA was 106.7-fold, 51.9-fold, and 437.8-fold higher, respectively, in the gills, digestive duct, and liver than in the muscles. The expression level of abalone Prx 2 mRNA in the liver peaked at 6 hr postinfection with Vibrio parahemolyticus and decreased at 12 hr postinfection. The expression level of abalone Prx 2 mRNA in hemocytes was drastically increased at 1 hr postinfection with V. parahemolyticus. These results suggest that abalone Prx 2 is conserved through evolution and that it may play a role similar to that of its mammalian counterpart.
Keywords
Abalone; Haliotis discuss hannai; peroxiredoxin; reactive oxygen species; Vibrio parahemolyticus;
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