• Title/Summary/Keyword: 초파리

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Effect of Popcorn, Wolbachia Variant, on Development and Reproduction in Drosophila melanogaster (Wolbachia 변이종 Popcorn이 초파리의 발육과 생식에 미치는 영향)

  • 한상미;민경태;한명세;이상철
    • Korean journal of applied entomology
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    • v.39 no.3
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    • pp.193-197
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    • 2000
  • Wolbachia, vertically transmitted bacterial endosymbionts, is known to induce cytoplasmic incompatibility (CI), parthenogenesis, or feminization in some insect species. The Wolbachia found to exist symbiotically as a non-virulent form in Drosophila melanogaster, however popcorn is a lethal strain of Wolbachia, which causes tissue degeneration and early death of its adult host. Popcorn-infected flies showed the delayed duration of an egg and larvae, and their pupal period and life span reduced. The oviposition and egg-hatching rate of popcorn-infected flies were decreased 15% and 80%, respectiv~ly, compared to those of the normal Wolbachia-infected flies. The pupation and emergence rates of popcorn-infected flies were 67% and 65%, respectively. When popcorn-infected flies were crossed with Wolba-chia-infected flies, and vice versa, both crosses resulted in a significant reduction in egg production, egg¬hatching, pupation, and emergence rate, and their progeny revealed the popcorn syndrome. When popcorn-infected male flies were crossed with uninfected females, popcorn was not detected in their progeny. Popcorn also maternally transmitted in flies, but it did not induce CI and affected on its host life cycle as a virulence.

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Effect of Plant Extracts with Superoxide Dismutase-like Activity on Survival of Fruit Flies under Oxidative Stress (Superoxide Dismutase유사활성을 지닌 식물체가 Oxidative Stress를 받고 있는 초파리의 수명에 미치는 영향)

  • Han, Dae-Seok;Kwak, Jae-Hyock;Kim, Sang-Hee;Kim, Seok-Joong
    • Korean Journal of Food Science and Technology
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    • v.28 no.5
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    • pp.865-869
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    • 1996
  • Biological effect of aqueous extracts of 12 plants which showed superoxide dismutase (SOD)-like activity in vitro was evaluated using Drosophila melanogaster. Survival percentage of the flies was a criterion of effect when the flies were exported to paraquat, which generated superoxide anion radical in vivo. When flies were co-administered with paraquat and aqueous extracts of garlic, lettuce, kiwi, and nameko, they showed no defensive effect against of oxygen toxicity. If flies were exposed to 60 mM paraquat after adaptation to feed containing plant extracts with SOD-like activity for 10 days, however, survival percentage of flies fed with phytochemicals was $35{\sim}63%$ while that percentage of flies fed without phytochemical was only 11%. This result indicated that adaptation of flies to plant extracts with SOD-like activity could prevent the flies from oxidative injury. On the other hand, lettuce, kiwi, nameko, onion, persimmon, fern brake and cauliflower showed a reparative effect on an oxidative stress. Dropwort, shiitake, agaric mushroom and broccoli did not show such an effect.

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A New Species , Microdrosophila chejuensis (Diptera ; Drosophilidae) from Korea (한국산 Microdrosophila 속 초파리 1 신종)

  • 이택준;김남우
    • Animal Systematics, Evolution and Diversity
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    • v.6 no.1
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    • pp.161-164
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    • 1990
  • One species of Korean Drosophiliae, Microdrosophila (Microdrosophila) Chejuensis , n. sp. was described as new to science and the male genitalia was illustrated. All seven species in the genus Microdrosophila including the new species are known to be distributed in Korea at present.

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Three New Species and Two Unrecorded Species of Drosophilidae from Korea (한국산 초파리과의 3 신종과 2미기록종)

  • 강영선;이택준;방규환
    • The Korean Journal of Zoology
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    • v.8 no.2
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    • pp.19-22
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    • 1965
  • Scaptomyza 속의 1 신종, drosophila 속의 2 신종의 기재와 Amiota 속의 한국미기록2 종을 기록했다. Scaptomyza(Scaptomyza) choi.sp.nov. 제주 -각씨 초파리(신칭) , Drosophila (Hirtodrosophila) nigripennis sp. nov. 검은톱니-초파리(신칭), Drosophila (Hirtodrosophila) pseudonokogiri sp.nov. 헛톱니-초파리(신칭) Amiota (Amiota) stylopyga WAKAHAMA& OKADA , 1958 Amiota (Amiota) dispina OKADA, 1960.

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Regulation of the Drosophila Fascin by Raf Signaling (Raf 신호에 의한 초파리 fascin의 조절)

  • Pyo, Jung-Hoon;Choi, Na-Hyun;Lee, Shin-Hae;Kim, Young-Shin;Yoo, Mi-Ae
    • Journal of Life Science
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    • v.19 no.7
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    • pp.866-870
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    • 2009
  • Fascin, a cytoskeleton actin binding protein, functions in cell adhesion and cell migration. Fascin is also known as a candidate biomarker for various cancers, however, regulatory mechanisms of fascin expression remains little understood. In this study, we found an abnormal bristle phenotype, which is similar to that of the Drosophila fascin mutant, in Drafmutant flies. Hence, we investigated whether fascin expression is regulated by Raf signaling. RT-PCR and Western blot analysis showed that Drosophila fascin expression was down-regulated in Draf mutant flies and the level was increased in larvae expressing the oncogenic form of Draf (Draf$^{got}$) under the GAL4-UAS system. Immunostaining analysis showed increased fascin in the hemocytes over-expressing Draf$^{got}$. Our results indicate that fascin expression is regulated by Raf signaling and suggest that Raf signaling may play an important role in the actin cytoskeleton-associated developmental process and tumor progression via regulation of fascin gene.

Newand Unrecorded Species of Drospphilidae in Korea (한국산 초파리과의 신종과 미기록종)

  • 이택준
    • The Korean Journal of Zoology
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    • v.7 no.2
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    • pp.37-40
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    • 1964
  • Drosophila 속의 2 신종이 기재와 Microdrosophila 속의 한국산미기록 3 종을 기록했다. Drosophila magnidentta sp.nov. 국명 : 큰니초파리(신칭) Drosophila takadai sp.nov. 국명 : 흐린별초파리(신칭) Microdrsophila (Microdrosophila ) fuscata OKADA, 1960 Microdrosophila (Microdrosophila) urashimae OKADA, 1960 Microdrosophila (Oxystyloptera) matsudairai OKADA , 1960

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Ecological Studies of Drosophila Populations in Korea (초파리집단의 생태학적 연구)

  • 이택준
    • The Korean Journal of Zoology
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    • v.5 no.1
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    • pp.13-20
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    • 1962
  • 1. 1957년 8월 7일부터 18일까지 계룡산에서 고도차분포를 조사하기 위하여 고도별 4 지점에서 과물 (복숭아, 도마도) trap를 사용하여 오전 6시와 오후 6시에 동시에 초파리를 채집하였다. 그리고 sweeping 도 하여 총 33 종을 채집하였는데 다음종들은 높은 지점에서 발견되었다. Microdrosophila purpurata, Mycodrosophila basalis, Parascaptomyz disticha, Scaptomyza aplicalis , Drosophila histrioides, D.difasciata, D.histrio. 그리고 초파리의 성비가 고도에 따라 변동하였음을 관찰하였는데 특히. D. suzukii 는 저지에서 충이 현저히 많았던 것이 700미 고지에서는 거의 1 : 1의 성비를 보여주었다. 300 미높이의 (B) 지점에서 D.lacertosa 와D. Coracina 의 일주기활동은 반대적이었는데 D.lacertosa 는 습도가 높은 아침에 주로 활동하고, D.coracina는 이와 반대로 주로 오후에 활동하고 있음을 알았다. 2. 초파리 집단의 계절적 변동을 조사하기 위하여 1958년 4월부터 11월까지 매일 중순삼일씩 공주잠업시험소구내 상전에서 그리고 동년 5월 터 11월까지는 매일 초순삼일식 계룡산에서 각각 5개의 과물 trap를 설치하고 비교관찰하엿따. 매월 초파리종들의 계절적 변동은 3 표와 4 표에 기재된 바와같다. 특히 D.auraria는 두장소에서 우점종이었는데 공주잠업시험소구내 상전에서는 9월에 최고에 달했고, 계룡산에서는 8월에 최고에 달하는 unimodality를 표시하였다. 3 . 초파리의 일주기활동을 조사하기 위하여1959년 6월부터 10월까지 공주잠업시험소구내 상전에서 매월 2일간씩 2시간마다 채집을 하였다. 이 지방에서 우점종인 D.auraria 는 매월 일주기활동에 있어서 bimodality를 형성하였는데 9, 10월에는 두 peak는 서로 근접되었다. 그리고 6월 26일 2시간마다 sweeping 법으로 채집한 D.auraria의 일주기활동도 비교연구하였는데 거의 같은 현상을 나타냈다.

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Validation of Domain Combination Based Protein-Protein Interaction Prediction Method Using Human and Fly Proteins (인간 및 초파리 단백질을 대상으로 한 도메인 조합 기반 단백질-단백질 상호작용 예측 기법 검증)

  • Jang Woo-Hyuk;Han Dong-Soo;Kim Hong-Soog;Lee Sung-Doke
    • Proceedings of the Korean Information Science Society Conference
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    • 2005.11b
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    • pp.235-237
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    • 2005
  • 도메인 조합 기반의 단백질-단백질 상호작용 예측 기법(DCPPIP)은 효모 단백질에 대하여 뛰어난 정확도를 보여준다. 그러나 다른 종에서의 예측 정확도 및 기법의 유효성은 아직까지 검증되지 않고 있다. 본 논문에서는, 초파리 및 인간 단백질을 이용한 예측 정확도 검증 및 이종간의 상호작용 예측 실험의 결과를 기술한다. 초파리와 인간 단백질의 실험에서는 각각 10,351개와 2,345개의 상호작용 단백질 쌍이 사용되었다. 초파리와 인간의 상호작용 단백질 쌍 중 $80\%$$20\%$를 각각 학습집단 및 실험집단으로 사용하였으며. 상호작용이 없는 단백질 쌍의 학습집단은 1배에서 5배까지 변화시키면서 예측 정확도를 관찰하였다. 정확도는 실험집단 중 학습집단과 도메인이 완전히 혹은 부분적으로 겹치는 쌍들에 대하여 계산하였다. 이 결과 초파리에서는 약 $77\%$의 민감도와 $92\%$의 특이도가 확인되었고 인간 단백질에 대하여는 약 $96\%$의 민감도와 $95\%$의 특이도를 보여주었다. 이종간의 상호작용 예측 실험은 효모, 초파리, 효모+초파리에 해당하는 학습집단 각각을 바탕으로 Human, Mouse, H. pylori, E. coli, C. elegans 등의 단백질 상호작용 예측을 수행하였다. 실험 결과 학습집단의 도메인이 실험집단의 도메인과 많이 겹칠 수륵 높은 정확도를 보여주었으며, 도메인 집단간의 유사도를 나타내기 위해 고안한 Domain Overlapping Rate(DOR)는 상호작용 예측 정확도의 중요한 요소임을 찾아 내었다.

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Mushrooms Utilized as Natural Breeding Sites by Wild Drosophilids on Jeju Island, Korea (야생초파리의 번식처로 이용되는 제주도산 버섯)

  • Kim, Won-Taek;Oh, Duck-Chul
    • The Korean Journal of Mycology
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    • v.31 no.3
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    • pp.134-140
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    • 2003
  • Wild Mushrooms collected in Mt. Halla of Jeiu Island around altitude of $200{\sim}1000\;m$ from 1999 to 2000 were studied to investigate utilization as the natural breeding sites by wild drosophilids. Thirty-five mushroom species belonging to the 19 genera of 12 families from 2 orders were used as natural breeding sites by drosophilids. Adult flies emerged from 14 species of mushrooms belonging to the 11 genera of 8 families from 2 orders were identified. From the rest of 21 species of mushrooms, only larvae or/and pupae were reaped. Among 35 species of mushrooms, 34 species belonged to order Agaricales and only one species, Sarcodon scabrosum, belonged to order Aphyllophorales. In terms of composition of mushroom families, total 35 species belonging to 12 families were composed of 10 species of Russulaceae, 6 species of Amanitaceae, 4 species of each Tricholomataceae and Agaricaceae, 3 species of Boletaceae, 2 species of Strophariaceae and each one species of rest 6 families. Concerning the composition of the genus, 35 species belonging to 11 genera were composed of 8 species of Russula, 6 species of Amanita, 4 species of Agaricus, 2 species of Lactarius, and each one species of rest 7 genera. In terms of the mushroomsize preference of drosophilid flies for breeding sites, most flies preferred comparatively large mushrooms above 5 cm of pileus size in diameter, Fourteen species of drosophilids were identified as mushroom-breeding species. Among these, one and five species belonged to genera Mycodrosophila and Hirtodrosophila respectively, The genus Drosophila included the most abundant 8 species. Drosophila bizonata and Hirtodrosophila sexvittata bred on 6 species of mushrooms respectively, D. unispina bred on 3 species of mushrooms, rest species of Drosophila bred on one or two species of mushrooms. From the result of this study and other literatures, it could be presumed that much more mushroom species could serve as the natural breeding sites for greater number of drosophilid species than those examined in this study on Jeju Island.

한국산 초파리 집단의 유전 생화학적 연구: 노랑 초파리의 $\alpha$-Glycerophosphate dehydrogenase allele에 대하여

  • 정용재;한영수;정영란
    • The Korean Journal of Zoology
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    • v.25 no.3
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    • pp.123-129
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    • 1982
  • In order to establish a biochemical genetic system in Drosophila populations in Korea, the $\\alpha$-glycerophosphate dehydrogenase alleles of eleven natural populations of D. melanogaster in Korea were examined by means of agarose gel electrophoresis. The results obtained are presented below: 1. $\\alpha$-Glycerophosphate dehydrogenase ($\\alpha$-GPDH) allele is scored for eleven natural populations of D. melanogaster in Korea, resulting that $\\alpha$-GPDH is found to be widely polymorphic for two electrophoretic variants. 2. The heterozygosity of $\\alpha$-GPDH is calculated as $40\\sim50%$. 3. The frequency of the FF genotype of $\\alpha$-GPDH is found to be roughly same as the SS genotype, but less than the FS genotype. 4. The F gene of $\\alpha$-GPDH is distributed almost frequently as the S gene.

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