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http://dx.doi.org/10.5352/JLS.2009.19.7.866

Regulation of the Drosophila Fascin by Raf Signaling  

Pyo, Jung-Hoon (Department of Molecular Biology, College of Natural Science, Pusan National University)
Choi, Na-Hyun (Department of Molecular Biology, College of Natural Science, Pusan National University)
Lee, Shin-Hae (Department of Molecular Biology, College of Natural Science, Pusan National University)
Kim, Young-Shin (Department of Molecular Biology, College of Natural Science, Pusan National University)
Yoo, Mi-Ae (Department of Molecular Biology, College of Natural Science, Pusan National University)
Publication Information
Journal of Life Science / v.19, no.7, 2009 , pp. 866-870 More about this Journal
Abstract
Fascin, a cytoskeleton actin binding protein, functions in cell adhesion and cell migration. Fascin is also known as a candidate biomarker for various cancers, however, regulatory mechanisms of fascin expression remains little understood. In this study, we found an abnormal bristle phenotype, which is similar to that of the Drosophila fascin mutant, in Drafmutant flies. Hence, we investigated whether fascin expression is regulated by Raf signaling. RT-PCR and Western blot analysis showed that Drosophila fascin expression was down-regulated in Draf mutant flies and the level was increased in larvae expressing the oncogenic form of Draf (Draf$^{got}$) under the GAL4-UAS system. Immunostaining analysis showed increased fascin in the hemocytes over-expressing Draf$^{got}$. Our results indicate that fascin expression is regulated by Raf signaling and suggest that Raf signaling may play an important role in the actin cytoskeleton-associated developmental process and tumor progression via regulation of fascin gene.
Keywords
Drosophila; fascin; singed; Raf; gene expression;
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1 Kureishy, N., V. Sapountzi, S. Prag, N. Anilkumar, and J. C. Adams. 2002. Fascins, and their roles in cell structure and function. Bioessays. 24, 350-361   DOI   ScienceOn
2 Melnick, M. B., L. A. Perkins, M. Lee, L. Ambrosio, and N. Perrimon. 1993. Developmental and molecular characterization of mutations in the Drosophila-raf serine/threonine protein kinase. Development 118, 127-138
3 Otto, J. J., R. E. Kane, and J. Bryan. 1979. Formation of filopodia in coelomocytes: localization of fascin, a 58,000 dalton actin cross-linking protein. Cell 17, 285-293   DOI   ScienceOn
4 Paterson, J. and K. O'Hare. 1991. Structure and transcription of the singed locus of Drosophila melanogaster. Genetics 129, 1073-1084
5 Patwari, P. and R. T. Lee. 2008. Mechanical control of tissue morphogenesis. Circ. Res. 103, 234-243   DOI   ScienceOn
6 Phelps, C. B. and A. H. Brand. 1998. Ectopic gene expression in Drosophila using GAL4 system. Methods 14, 367-379   DOI   ScienceOn
7 Ramos, J. W. 2008. The regulation of extracellular signalregulated kinase (ERK) in mammalian cells. Int. J. Biochem. Cell Biol. 40, 2707-2719   DOI   ScienceOn
8 Xie, J., J. Qian, J. Yang, S. Wang, M. E. Freeman 3rd, and Q. Yi. 2005. Critical roles of Raf/MEK/ERK and PI3K/AKT signaling and inactivation of p38 MAP kinase in the differentiation and survival of monocyte-derived immature dendritic cells. Exp. Hematol. 33, 564-572   DOI   ScienceOn
9 Bryan, J., R. Edwards, P. Matsudaira, J. Otto, and J. Wulfkuhle. 1993. Fascin, an echinoid actin-bundling protein, is a homolog of the Drosophila singed gene product. Proc. Natl. Acad. Sci. U. S. A. 90, 9115-9119   DOI   ScienceOn
10 Buda, A. and M. Pignatelli. 2004. Cytoskeletal network in colon cancer: from genes to clinical application. Int. J. Biochem. Cell Biol. 36, 759-765   DOI   ScienceOn
11 Cant, K., B. A. Knowles, M. S. Mooseker, and L. Cooley. 1994. Drosophila singed, a fascin homolog, is required for actin bundle formation during oogenesis and bristle extension. J. Cell Biol. 125, 369-380   DOI   ScienceOn
12 De Arcangelis, A., E. Georges-Labouesse, and J. C. Adams. 2004. Expression of fascin-1, the gene encoding the actin-bundling protein fascin-1, during mouse embryogenesis. Gene Expr. Patterns 4, 637-643   DOI   ScienceOn
13 Howe, A. K., A. E. Aplin, and R. L. Juliano. 2002. Anchorage-dependent ERK signaling--mechanisms and consequences. Curr. Opin. Genet. Dev. 12, 30-35   DOI   ScienceOn
14 Guan, Y., P. L. Woo, N. M. Rubenstein, and G. L. Firestone. 2002. Transforming growth factor-alpha abrogates the glucocorticoid stimulation of tight junction formation and reverses the steroid-induced down-regulation of fascin in rat mammary epithelial tumor cells by a Ras-dependent pathway. Exp. Cell Res. 273, 1-11   DOI   ScienceOn
15 Hashimoto, Y., M. Parsons, and J. C. Adams. 2007. Dual actin-bundling and protein kinase C-binding activities of fascin regulate carcinoma cell migration downstream of Rac and contribute to metastasis. Mol. Biol. Cell 18, 4591-4602   DOI   ScienceOn
16 Helfman, D. M. and G. Pawlak. 2005. Myosin light chain kinase and acto myosin contractility modulate activation of the ERK cascade downstream of oncogenic Ras. J. Cell Biochem. 95, 1069-1080   DOI   ScienceOn
17 Kolch, W. 2005. Coordinating ERK/MAPK signalling through scaffolds and inhibitors. Nat. Rev. Mol. Cell Biol. 6, 827-837   DOI   ScienceOn
18 Asha, H., I. Nagy, G. Kovacs, D. Stetson, I. Ando, and C. R. Dearolf. 2003. Analysis of Ras-induced overproliferation in Drosophila hemocytes. Genetics 163, 203-215
19 Adams, J. C. 2004. Roles of fascin in cell adhesion and motility. Curr. Opin. Cell Biol. 16, 590-596   DOI   ScienceOn
20 Adams, J. C., J. D. Clelland, G. D. Collett, F. Matsumura, S. Yamashir, and L. Zhang. 1999. Cell-matrix adhesions differentially regulate fascin phosphorylation. Mol. Biol. Cell 10, 4177-4190   DOI   ScienceOn
21 Brand, A. H. and N. Perrimon. 1993. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development. 118, 401-415
22 Bros, M., X. L. Ross, A. Pautz, A. B. Reske-Kunz, and R. Ross. 2003. The human fascin gene promoter is highly active in mature dendritic cells due to a stage-specific enhancer. J. Immunol. 171, 1825-1834   DOI