• Title/Summary/Keyword: 초저온냉동

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Continuous-Flow Analysis for Determination of Nitrate Using Hydrazine-Copper Method in Plan (Hydrazine-Copper 방법을 이용한 연속흐름제어장치를 통한 식물체의 nitrate 분석)

  • Park, Yang-Ho;Park, So-Hyeon;Lee, Ju-Young;Jang, Byoung-Choon;Lee, Ki-Sang
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.4
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    • pp.204-208
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    • 2006
  • This study is to describe continuous-flow analysis (CFA) for the determination of nitrate using hydrazine-copper in plant material and to test precision of this method compared with that of methods, which are RQflex method and salicylic acid method. Samples were leaves of watermelon, cucumber, melon and tomato. Nitrate values measured by the RQflex method were greater than those measured by CFA or salicylic acid method. The correlation of nitrate values between those measured by CFA and salicylic acid method was $R^2=0.9671$, and those measured by CFA between those measured by RQflex method was $R^2=0.9739$. Recovery rate of nitrate added to tissue extract by CFA method was $99.7{\pm}0.25%$.

Soft Start-up Algorithm of Single-Phase Induction Motor Based on Full-bridge Inverter (풀 브리지 인버터 기반 단상 유도전동기의 소프트 기동 기법)

  • Kim, Tae-Seong;Kang, Hyung-Do;Hwang, Seon-Hwan
    • Journal of IKEEE
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    • v.22 no.2
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    • pp.258-265
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    • 2018
  • This paper proposes an algorithm for reducing the starting current when the single-phase induction motor starts and analyzes its operation. Generally, the single-phase induction motors require several starters to generate the starting torque due to their structural characteristics. In this paper, a capacitor-start / capacitor-run method of the single-phase induction motor is basically adopted. This conventional method is efficient and has a large starting torque, but it generates about 5 ~ 6 times of inrush current at startup. As a result, the freezer starting device and peripheral devices are damaged and life time may be reduced. To reduce the inrush current, the current control algorithm based on the virtual dq model is presented to control the starting current. In addition, it validates the proposed algorithm through experiments to smooth transit from start-up operation to the rated operating region.

Cryogenic Distillation Simulation for Hydrogen Isotopes Separation (수소 동위원소 분리를 위한 초저온증류공정 모사)

  • Noh, Sanggyun;Rho, Jaehyun;Cho, Jungho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.9
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    • pp.4643-4651
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    • 2013
  • In this study, we have surveyed the new technologies in the cryogenic distillation of ITER, equilibrium reactors and helium refrigeration cycle contained in the isotope separation system (ISS). We also have collected thermodynamic and transport properties for $H_2$, HD, $D_2$, HT, DT and $T_2$ components of which properties are not built in a general purpose chemical process simulators such as Aspen Plus and PRO/II with PROVISION. Verification works have been performed to compare between literature data and simulation results. For the simulation of ISS involving six hydrogen isotope components, four distillation columns and two equilibrium reactors are used for the separation of $D_2$ and DT from $T_2$.

Development of Rabbit Brain Tumor Model Using VX2 Cells and Verification with the MRI in Neuroradiologic Research (Neuroradiology 연구를 위한 VX2 세포를 이용한 토끼 뇌종양 모델 제작과 MRI를 이용한 검증)

  • Yong-Woo Kim;Seon Hee Choi;Hak Jin Kim
    • Journal of the Korean Society of Radiology
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    • v.84 no.2
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    • pp.441-453
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    • 2023
  • Purpose To evaluate the development, location, and volume of a VX2 carcinoma using four inoculation methods in a rabbit brain. Materials and Methods Inoculation of a VX2 cell suspension was performed 1) on the appointed day, 2) seven days after storing a VX2 carcinoma in a freezer or 3) seven days after storing a VX2 carcinoma in a deep freezer after sacrificing the donor rabbits. 4) Without sacrificing the rabbits, the VX2 cell suspension was obtained using a gun biopsy, inoculation was performed on the appointed day. MR imaging was performed 10 days after inoculation. Brain tissues were obtained the day after. The development, location, and volume of the tumor were evaluated. Results Seventeen of the 18 rabbits inoculated on the appointed day developed tumors (average tumor volume, 106.32 mm3). One of five inoculated seven days after storing the VX2 tumor in the freezer, and three of five inoculated seven days after storing the VX2 tumor in the deep freezer developed tumors. Inoculation with a VX2 cell suspension obtained with a gun biopsy from five rabbits revealed development of tumors in only two rabbits. The tumors mostly developed in the superficial cortex. Conclusion TVX2 rabbit brain tumor model is easy to develop and revealed variable reproducibility. This model can be applicable in radiologic imaging, treatment planning, interventional treatment and drug delivery research. VX2 cell can be successfully innoculated into the brain using variable methods under researcher's variable conditions.

Development of Analytical Reference Material for Proficiency Test of Pesticide Multi-residue Analysis in Green-pepper (풋고추 농약다성분분석 정도관리용 분석표준물질 개발)

  • Kim, Jong-Hwan;Choi, Sung-Gil;Oh, Young-Gon;Kwon, Young-Sang;Hong, Su-Myeong;Sung, Mun-Hyun;Lee, Se-Ja;Hwang, Sun-Young;Seo, Jong-Su
    • The Korean Journal of Pesticide Science
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    • v.20 no.3
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    • pp.211-220
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    • 2016
  • This study was to develop the analytical reference material of green-pepper for multi-residue analysis of pesticides. According to the ISO Guide 35, ISO Guide 13528 and EURL-PT protocol, the homogeneity, stability, assigned value and uncertainty were calculated to assess if it was suitable to be used as the proficiency test or quality control. The values of the within-bottle standard variation ($s_{wb}$) and the between-bottle standard variation ($s_{bb}$) were 1.7~3.7% of assigned value according to the requirement of the ISO guide 35. And, the uncertainty ($u^*{_{bb}}$) due to inhomogeneity was 0.8~1.1% for all pesticides. The storage stabilities of ten-pesticides at various conditions were assessed. For all target pesticides, the slop ($b_1$) values were smaller than the corresponding values of $[t_{0.95,n-2}{\times}s(b_1)]$ specified by the ISO guide 35, indicating that there were no statistically significant decreases in the concentration of the target pesticides when the analytical reference material was stored at room temperature ($20{\sim}30^{\circ}C$) for 7 days, freezing ($-20^{\circ}C$) for 30 days and deep freezer ($-80^{\circ}C$. except for bifenthrin, fenpropathrin) for 245 days. For proficiency test by using it developed by Korea Institute of Toxicology, inter-lab test was performed with eight organization performing the residual pesticide analysis. We found that there were some different results among them. Some were assessed as questionable or unacceptable for two pesticides and one organization didn't analyze the six pesticides. From these results, this green-pepper analytical reference material containing ten-pesticides could be used as a tool for the proficiency test to improve the reliability or consistency for pesticide residue's results.

Evaluation of a Rapid Diagnostic Antigen Test Kit Ribotest Mycoplasma® for the Detection of Mycoplasma pneumoniae (Mycoplasma pneumoniae 감염의 신속 항원 검사 키트 "Ribotest Mycoplasma®"의 진단적 평가)

  • Yang, Song I;Han, Mi Seon;Kim, Sun Jung;Lee, Seong Yeon;Choi, Eun Hwa
    • Pediatric Infection and Vaccine
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    • v.26 no.2
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    • pp.81-88
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    • 2019
  • Purpose: Early detection of Mycoplasma pneumoniae is important for appropriate antimicrobial therapy in children with pneumonia. This study aimed to evaluate the diagnostic value of a rapid antigen test kit in detecting M. pneumoniae from respiratory specimens in children with lower respiratory tract infection (LRTI). Methods: A total of 215 nasopharyngeal aspirates (NPAs) were selected from a pool of NPAs that had been obtained from children admitted for LRTI from August 2010 to August 2018. The specimens had been tested for M. pneumoniae by culture and stored at $-70^{\circ}C$ until use. Tests with Ribotest $Mycoplasma^{(R)}$ were performed and interpreted independently by two investigators who were blinded to the culture results. Results: Among the 215 NPAs, 119 were culture positive for M. pneumoniae and 96 were culture negative. Of the culture-positive specimens, 74 (62.2%) were positive for M. pneumoniae by Ribotest $Mycoplasma^{(R)}$, and 92 of the 96 (95.8%) culture-negative specimens were negative for M. pneumoniae by Ribotest $Mycoplasma^{(R)}$. When culture was used as the standard test, the sensitivity and specificity of Ribotest $Mycoplasma^{(R)}$ were 62.2% and 95.8%, respectively. Additionally, the positive predictive value, negative predictive value, and overall agreement rates with Ribotest $Mycoplasma^{(R)}$ were 94.9%, 67.2%, and 77.2%, respectively. Conclusions: A positive test result of Ribotest $Mycoplasma^{(R)}$ suggests a high likelihood of culture-positive M. pneumoniae infection. However, a negative test result should be interpreted with caution because nearly one-third of negative test results reveal culture-positive M. pneumoniae infections.

Diagnostic Evaluation of the BioFire® Meningitis/Encephalitis Panel: A Pilot Study Including Febrile Infants Younger than 90 Days (BioFire® Meningitis/Encephalitis Panel의 진단적 유용성 평가: 90일 미만 발열영아에서의 예비 연구)

  • Kim, Kyung Min;Park, Ji Young;Park, Kyoung Un;Sohn, Young Joo;Choi, Youn Young;Han, Mi Seon;Choi, Eun Hwa
    • Pediatric Infection and Vaccine
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    • v.28 no.2
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    • pp.92-100
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    • 2021
  • Purpose: Rapid detection of etiologic organisms is crucial for initiating appropriate therapy in patients with central nervous system (CNS) infection. This study aimed to evaluate the diagnostic value of the BioFire® Meningitis/Encephalitis (ME) panel in detecting etiologic organisms in cerebrospinal fluid (CSF) samples from febrile infants. Methods: CSF samples from infants aged <90 days who were evaluated for fever were collected between January 2016 and July 2019 at the Seoul National University Children's Hospital. We performed BioFire® ME panel testing of CSF samples that had been used for CSF analysis and conventional tests (bacterial culture, Xpert® enterovirus assay, and herpes simplex virus-1 and -2 polymerase chain reaction) and stored at -70℃ until further use. Results: In total, 72 (24 pathogen-identified and 48 pathogen-unidentified) CSF samples were included. Using BioFire® ME panel testing, 41 (85.4%) of the 48 pathogen-unidentified CSF samples yielded negative results and 22 (91.7%) of the 24 pathogen-identified CSF samples yielded the same results (enterovirus in 19, Streptococcus agalactiae in 2, and Streptococcus pneumoniae in 1) as those obtained using the conventional tests, thereby resulting in an overall agreement of 87.5% (63/72). Six of the 7 pathogen-unidentified samples were positive for human parechovirus (HPeV) via BioFire® ME panel testing. Conclusions: Compared with the currently available etiologic tests for CNS infection, BioFire® ME panel testing demonstrated a high agreement score for pathogen-identified samples and enabled HPeV detection in young infants. The clinical utility and cost-effectiveness of BioFire® ME panel testing in children must be evaluated for its wider application.