Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
권호 표지

Continuous-Flow Analysis for Determination of Nitrate Using Hydrazine-Copper Method in Plan

Hydrazine-Copper 방법을 이용한 연속흐름제어장치를 통한 식물체의 nitrate 분석

  • Park, Yang-Ho (National Institute of Agricultural Science and Technology) ;
  • Park, So-Hyeon (National Institute of Agricultural Science and Technology) ;
  • Lee, Ju-Young (National Institute of Agricultural Science and Technology) ;
  • Jang, Byoung-Choon (National Institute of Agricultural Science and Technology) ;
  • Lee, Ki-Sang (National Institute of Agricultural Science and Technology)
  • Received : 2005.11.02
  • Accepted : 2006.02.05
  • Published : 2006.08.28
Download PDF
⟨ Previous Next ⟩

Abstract

This study is to describe continuous-flow analysis (CFA) for the determination of nitrate using hydrazine-copper in plant material and to test precision of this method compared with that of methods, which are RQflex method and salicylic acid method. Samples were leaves of watermelon, cucumber, melon and tomato. Nitrate values measured by the RQflex method were greater than those measured by CFA or salicylic acid method. The correlation of nitrate values between those measured by CFA and salicylic acid method was $R^2=0.9671$, and those measured by CFA between those measured by RQflex method was $R^2=0.9739$. Recovery rate of nitrate added to tissue extract by CFA method was $99.7{\pm}0.25%$.

이연구의 목적은 연속흐름제어장치를 통한 식물체의 nitrate 분석방법 및 RQflex 방법 및 salicylic acid 방법과 비교를 통해 그 정확성을 확인하고자 함에 있다. 분석은 메론, 수박, 오이, 그리고 토마토 잎을 현장에서 채취 후, 분석시까지 초저온 냉동고에 보관후 분석하였다. RQflex로 측정한 결과가 CFA방법이나 salicylic acd 방법보다 약간 높게 나왔으며, CFA방법과 salicylic acid 방법과의 상관관계는 $R^2=0.9671$을 보였으며, RQflex와의 상관관계는 $R^2=0.9739$를 보였다.

Keywords

References

  1. Alves, B. J.. R, L., Zotarelli, A.S Resende, J.C. Polidoro, S. Urquigara, and R. M. Boddey. 2000. Rapid and sensitive determination of nitrate in plant tissue using flow injection analysis. Commumnicaion of Soil Sci. Plant Anal. 31 : 2739-2750 https://doi.org/10.1080/00103620009370623
  2. AOAC method .1995. Official methods of analysis 16th edn. AOAC, pp8-9
  3. Bertotti, M., and D. Pletcher .1997. Amperometric determination of nitrite via reaction with iodide using microelectrodes. Anal. Chin. Acta. 337 : 49-55 https://doi.org/10.1016/S0003-2670(96)00431-X
  4. Brinkered, E. F., and O. E. Kolari. 1975. The history and use of nitrite and nitrate in the curing of meet. Food Cosmo Toxi. 13: 655-661 https://doi.org/10.1016/0015-6264(75)90157-1
  5. Buttv S. B., M. Riaz, and M. Z. Iqbal .2001. Simulaneous determination of nitrate in the curing of meet. Food and Cosmetics Toxicology. 13 : 655-661 https://doi.org/10.1016/0015-6264(75)90157-1
  6. Cataldo, D.A, M. Haroon, L.E. Schrader, and V.L. Youngs. 1975. Rapid colorimetric determination of nitrate in plant tissue. Comm. Soil Sci. Plant Anayl. 6 : 71-80 https://doi.org/10.1080/00103627509366547
  7. Haghighi, B., and A. Tavassoli. 2001. Flow-injection analysis of nitrate by reduction to nitrite and gas-phase molecular absorption spectrometry. Fresenius J. Anal. Chem. 371: 1113-1118 https://doi.org/10.1007/s002160101070
  8. Liang, B., M. Iwatsuki, and T. Fukasawa. 1994. Catalylic spectrophotometric determination of nitrite using the chlorpromazine hydrogen-peroxide dedox reaction in acetic-acid medium. Analyst. 119: 2114-2117
  9. Li, J. Z., X. C. Wu, R.Yuan, H. G .. Lin and R. Q.Yu .1994. Cobalt phthalocyanine derivatives as neutral carriers for nitrite-sensitive poly (vinyl chloride) membrane electrodes. Analyst. 119 : 1363-1366 https://doi.org/10.1039/an9941901363
  10. MAFF. Ministry of Agriculture, Fisheries and Food .1992. Nitrate and nitrite and N-niroso compounds in food: second report, Food surveillance paper 32
  11. Mattew J. M., D. James, G. C. Richard. 2001. Detection and determination of nitrate and nitrite: a review. Talanta. 54 : 785-803 https://doi.org/10.1016/S0039-9140(01)00323-X
  12. Oztekin, N., M.S. Nuku and F. B. Erim .2002. Simultaneous determination of nitrite and nitrate in meat products and vegetables by capillary electrophoresis. Food Chem. 76 : 103-106 https://doi.org/10.1016/S0308-8146(01)00287-4