• Title/Summary/Keyword: 초기희석

Search Result 225, Processing Time 0.023 seconds

New Adaptation Process to Serum Free Media for the Growth of Serum-requiring Human Cell Line (혈청 요구성 세포의 생육을 위한 무혈청 배지의 새로운 적응 공법)

  • 김영남;유호금
    • KSBB Journal
    • /
    • v.7 no.2
    • /
    • pp.132-138
    • /
    • 1992
  • Sprial adaptation technique of conditioned media has been applied to cultivate human cell line which can not survive in a serum free mdium without adding any growth factors in basal medium Doubling time and scu-PA production from serum free adapted cells were 5 days and 890 (IU/mL), respectively in a T-flask, whose values were not much lower than the productivity of 1100(IU/mL) from 5% serum containing medium. It was required to use conditioned media for attaching cells on microcarriers when cells were inoculated into a spinner vessel. Then, cells could continuously grow in serum free medium with having specific growth rate of 0.106 (1/day) and specific scu-PA production rate of $1.58{\times}10_{-5}$(IU/cell/day) in batch cultivation.

  • PDF

Preparation of α-Si3N4 Powder, in Reaction System Containing Molten Salt, by SHS (Part 3. Reaction Mechanism) (용융염계에서 자전연소합성법에 의한 α-Si3N4 분말의 제조 (Part 3. 반응기구))

  • Yun, Ki-Seok;Yang, Beom-Seok;Park, Young-Cheol;Won, Chang-Whan
    • Journal of the Korean Ceramic Society
    • /
    • v.41 no.12 s.271
    • /
    • pp.907-914
    • /
    • 2004
  • The nitridation mechanism of Si by SHS at $Si-NaCl-NH_{4}Cl-NaN_3$ system was investigated in this work. It was revealed that NaCl as a diluent was helpful to the perfect nitridation reaction by retarding the growth of Si particle resulted from the melting of Si at the initial stage of the nitridation reaction. And $NH_{4}Cl\;and\;NaN_3$ formed NaCl through decomposition and combination, and the preheating of pellet was helpful to the nitridation reaction in this process. The main nitridation mechanism of this system was liquid-gas reaction. The optimum porosity of the pellet for the nitridation of ${\alpha}-Si_{3}N_4$ was $67-69\%$.

A Study on Industrial Media for Production of Lactic acid in Batch and Continuous Fermentations (회분식 및 연속배양에 있어서 고농도 젖산의 생산을 위한 공업용 배지연구)

  • 김양훈;이기범;문승현
    • KSBB Journal
    • /
    • v.14 no.2
    • /
    • pp.181-187
    • /
    • 1999
  • We have investigated industrial media for lactic acid fermentation to reduce the cost of nitrogen sources. Corn steep liquor (CSL) was successfully used at 5% (v/v) in batch fermentations. Use of soluble CSL improved the productivity about 20% with an advantage of clearer fermentation broth. Yeast extract-complemented CSL improved the productivity about 20% with an advantage of clearer fermentation broth. Yeast extract-complemented CSL media further increased the increased the productivity. It was found that 3.1 g/L yeast extract and 5% CSL could be an effective substitute for 15 g/L yeast extract in 10% glucose medium. Brewing yeast was also used as a sole nitrogen source equivalent to 5% CSL. A continuous culture coupled with cell-recycle by microfiltration at the dilution rate of 0.05-0.065 h-1 led to the highest lactic acid productivity. Lactic acid was recovered by electrodialysis from the cell free broth. Depleted cell free broth supplemented with 5-10 g/L of yeast extract performed reasonably in batch and continuous cultures. Reuse of the fermentation broth may reduce the cost of raw materials as well as minimize the fermentation wastes.

  • PDF

A study on the measurement of thermophysical properties of ${Al}_{2}{O}_{3}, {Si}_{3}{N}_{4}$ and SiC series by a single rectangular pulse heating (방향파 펄스 가열에 의한 ${Al}_{2}{O}_{3}, {Si}_{3}{N}_{4}$, SiC 계열의 열물성치 측정에 관한 연구)

  • 차경옥;장희석;이흥주
    • Transactions of the Korean Society of Mechanical Engineers
    • /
    • v.14 no.1
    • /
    • pp.145-156
    • /
    • 1990
  • In this study, thermophysical properties of the engineering ceramic materials such as $Al_{2}$O$_{3}$, Si$_{3}$N$_{4}$ and SiC were measured b y a single rectangular pulse heating method. The values of thermal diffusivities, specific heats, and thermal conductivities were measured as a function of temperature ranging form room temperature to 1300K. The measured thermal properties of one group of ceramic material were compared with those of other group and discussed in detail in connection with the chemical composition. Thus, some criteria for thermal design with the engineering ceramic materials were proposed.

An Experimental Study for the Hydraulic Behavior of Artificial Rock Joint under Compression and Shear Loading (압축과 전단 하중을 받는 인공 암석 절리의 수리적 거동에 관한 실험적 연구)

  • 이희석;박연주;유광호;이희근
    • Tunnel and Underground Space
    • /
    • v.10 no.1
    • /
    • pp.45-58
    • /
    • 2000
  • Cyclic shear test system, which is capable of measuring flow rate inside rock joint, was established to investigate the hydraulic behavior of rough rock joints under various loading conditions. Laboratory hydraulic tests during compression and shear were conducted for artificial rough rock joints. Prior to tests, aperture characteristics of specimens were examined by measuring surface topography. Permeability changes under compression were well approximated with several hydraulic model. Hydraulic behavior conformed to dilation characteristics in the first stage, and permeability increased with increase of dilation. As the shear displacement progressed, flow rate became somewhat constant due to gouge production and offset of apertures. Hydraulic behavior under cyclic shear loading was also influenced by the degradation of asperities and gouge production. In addition. the relation between hydraulic aperture and mechanical aperture under compression and shear loading was investigated and compared.

  • PDF

Relationship between Softwood Lumber Thicknesses and Drying Rate and Drying Time Factor in Vacuum-Press Drying (가열판 압체식 진공건조에서 침엽수 제재두께와 건조속도 및 건조시간계수의 관계)

  • Jung, Hee-Suk;Lee, Jun-Ho;Kang, Wook;Lee, Nam-Ho
    • Journal of the Korean Wood Science and Technology
    • /
    • v.29 no.1
    • /
    • pp.1-8
    • /
    • 2001
  • Red pine (Pinus densiflora), white pine (Pinus koraiensis), larch (Larix leptolepis) and Western hemlock (Tsuga heterophylla) lumber were vacuum-press dried under three drying schedules to investigate current moisture contents (MC), drying rates and drying time factors related to five lumber thicknesses of 3, 5, 7, 9 and 11cm. Current MC could be estimated by drying factors such as lumber thickness, initial Me and drying time. Average drying rate from 30% to 15% Me was the highest for Western hemlock below fiber satuartion point, red pine, white pine and larch in the order of their magnitude. Drying rate curvilinearly decreased as lumber thickness increased. Drying time factor curvilinearly increased as lumber thickness increased.

  • PDF

Thermophilic Anaerobic Digestion of Animal Carcasses (동물 사체의 고온 혐기성 소화)

  • Kim, Sang-Hyoun;Sung, Shi-Hwu
    • Journal of the Korea Organic Resources Recycling Association
    • /
    • v.16 no.1
    • /
    • pp.31-38
    • /
    • 2008
  • Animal carcasses have always been and continue to be a major burden in animal production. The main aim of this study is to evaluate the feasibility of thermophilic anaerobic digestion for animal carcasses. A batch test using ground meat and organ as the model substrate showed that animal carcasses arehighly biodegradable at thermophilic anaerobic condition. The volatile solids (VS) destruction and $CH_4$ yieldranged from 52.7 to 58.5% and from 220 to 243 mL/g VS, respectively, at initial substrate VS in the range of 1.5~7.7%. However, high ammonia concentration inhibited continuous operation at substrate VS above 2.5%. As ammonia is formed during the degradation of proteineous organic materials, the major constituent of animal carcasses, the only way to reduce the ammonia concentration would be dilution. Co-digestion with other waste stream without high nitrogen content is recommended as an economically feasible approach for thermophilic digestion of animal carcass.

  • PDF

Evaluation of Interpretability for Generated Rules from ANFIS (ANFIS에서 생성된 규칙의 해석용이성 평가)

  • Song, Hee-Seok;Kim, Jae-Kyeong
    • Journal of Intelligence and Information Systems
    • /
    • v.15 no.4
    • /
    • pp.123-140
    • /
    • 2009
  • Fuzzy neural network is an integrated model of artificial neural network and fuzzy system and it has been successfully applied in control and forecasting area. Recently ANFIS(Adaptive Network-based Fuzzy Inference System) has been noticed widely among various fuzzy neural network models because of outstanding performance of control and forecasting accuracy. ANFIS has capability to refine its fuzzy rules interactively with human expert. In particular, when we use initial rule structure for machine learning which is generated from human expert, it is highly probable to reach global optimum solution as well as shorten time to convergence. We propose metrics to evaluate interpretability of generated rules as a means of acquiring domain knowledge and compare level of interpretability of ANFIS fuzzy rules to those of C5.0 classification rules. The proposed metrics also can be used to evaluate capability of rule generation for the various machine learning methods.

  • PDF

Development of Technology Mapping Algorithm for CPLD by Considering Time Constraint (시간제약 조건을 고려한 CPLD 기술 매핑 알고리즘 개발)

  • Kim, Hi-Seok;Byun, Sang-Zoon
    • Journal of the Korean Institute of Telematics and Electronics C
    • /
    • v.36C no.6
    • /
    • pp.9-17
    • /
    • 1999
  • In this paper, we propose a new technology mapping algorithm for CPLD under time constraint. In our technology mapping algorithm, a given logic equation is constructed as the DAG type, then the DAG is reconstructed by replicating the node that outdegree is more than or equal to 2. As a result, it makes delay time and the number of CLBs to be minimized. Also, after the number of multi-level is defined and cost of each nodes is calculated, the graph is partitioned in order to fit to k that is the number of OR term within CLB. The partitioned nodes are merged through collapsing and bin packing is performed in order to fit to the number of OR term within CLB. In the results of experiments to MCNC circuits for logic synthesis benchmark, we can shows that proposed technology mapping algorithm reduces delay time and the number of CLBs much more than the existing tools of technology mapping algoritm.

  • PDF

The Characteristics of Carbon Dioxide Fixation by Chlorella sp. HA-1 in Semi-continuous Operation (반연속식 운전에서 Chlorella sp. HA-1의 이산화탄소 고정화 특성)

  • Lee, Jae-Young;Kang, Hyun-Ah;Yang, Ji-Won
    • KSBB Journal
    • /
    • v.14 no.6
    • /
    • pp.742-746
    • /
    • 1999
  • The microalgal, Chlorella sp. HA-1, had good $CO_2$ fixation efficiency compared to other algal strains at the same operating condition. In this study, Chorella sp. HA-1 showed similar tolerance both 10% and 20% $CO_2$ concentration. By optimization of the major operation variables such as pH, initial cell concentration, light intensity, the $CO_2$ fixation rate could be raised to a reasonably high value, 372 $gCO_2/m^2{\cdot}day$ in a 3 L internally illuminated photobioreactor. In order to maintain the $CO_2$ fixation rate for a long time, the method of semi-continuous operation was employed, in which dilution ratio was the controlling parameter. Starting with the dilution ratio of 0.5 with the increased increment of 0.1, the constant $CO_2$ fixation rate was obtained.

  • PDF