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Effect of Different Nutrient Solution and Light Quality on Growth and Glucosinolate Contents of Watercress in Hydroponics (배양액의 종류 및 광질이 물냉이의 생육 및 Glucosinolate 함량에 미치는 영향)

  • Choi, Jae Yun;Kim, Sung Jin;Bok, Kwon Jeong;Lee, Kwang Ya;Park, Jong Seok
    • Journal of Bio-Environment Control
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    • v.27 no.4
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    • pp.371-380
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    • 2018
  • Aim of this study was to investigate the effects of different nutrient solutions and various light qualities generated by LED on the growth and glucosinolates contents of watercress (Nasturtium officinale) grown under hydroponics for 3 weeks. The seeds of watercress were sown on crushed rockwool media and raised them for two weeks. They were transplanted in a semi-DFT (deep flow technique) hydroponics system. A controlled-environment room was maintained at $20{\pm}1^{\circ}C$ and $16{\pm}1^{\circ}C$ temperatures and $65{\pm}10%$ and $75{\pm}10%$ relative humidity (day and night, respectively), with a provided photosynthetic photon flux density (PPFD) of $180{\pm}10{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and a photoperiod of 16/8h. To find out the best kinds of nutrient solutions for growing watercress, Otsuka House 1A (OTS), Horticultural Experiment Station in Korea (HES), and Netherland's Proefstaion voor Bloemisterij en Gasgroente (PBG) were adapted with initial EC of $1.0-1.3dS{\cdot}m^{-1}$ and pH of 6.2, irradiating PPFD with fluorescent lamps (Ex-1). Either monochromatic (W10 and R10) or mixed LEDs (R5B1, R3B1, R2B1G1, and W2B1G1) were irradiated with a differing ratio of each LED's PPFD to understanding light quality on the growth and glucosinolates contents of watercress (Ex-2). Although significant difference in the shoot growth of watercress was not found among three nutrient solutions treatments, but the root fresh weight increased by 13.7% and 55.1% in PBG and OTS compared to HES, respectively. OTS increased the gluconasturtiin content by 96% and 65% compared to PBG and HES. Compared with the white light (W10), the red light (R10) showed a 101.3% increase in the shoot length of watercress. Increasing blue light portion positively affected plant growth. The content of total glucosinolates in watercress was increased by 144.5% and 70% per unit dry weight in R3B1 treatment compared with R2B1G1 and W10 treatments, respectively. The growth and total glucosinolates contents of the watercress were highest under R3B1 among six light qualities.

Transfer of Isolated Mitochondria to Bovine Oocytes by Microinjection (미세주입을 이용한 난자로의 분리된 미토콘드리아 전달)

  • Baek, Sang-Ki;Byun, June-Ho;Kim, Bo Gyu;Lee, A ram;Cho, Young-Soo;Kim, Ik-Sung;Seo, Gang-Mi;Chung, Se-Kyo;Lee, Joon-Hee;Woo, Dong Kyun
    • Journal of Life Science
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    • v.27 no.12
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    • pp.1445-1451
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    • 2017
  • Mitochondria play a central role in energy generation by using electron transport coupled with oxidative phosphorylation. They also participate in other important cellular functions including metabolism, apoptosis, signaling, and reactive oxygen species production. Therefore, mitochondrial dysfunction is known to contribute to a variety of human diseases. Furthermore, there are various inherited diseases of energy metabolism due to mitochondrial DNA (mtDNA) mutations. Unfortunately, therapeutic options for these inherited mtDNA diseases are extremely limited. In this regard, mitochondrial replacement techniques are taking on increased importance in developing a clinical approach to inherited mtDNA diseases. In this study, green fluorescence protein (GFP)-tagged mitochondria were isolated by differential centrifugation from a mammalian cell line. Using microinjection technique, the isolated GFP-tagged mitochondria were then transferred to bovine oocytes that were triggered for early development. During the early developmental period from bovine oocytes to blastocysts, the transferred mitochondria were observed using fluorescent microscopy. The microinjected mitochondria were dispersed rapidly into the cytoplasm of oocytes and were passed down to subsequent cells of 2-cell, 4-cell, 8-cell, morula, and blastocyst stages. Together, these results demonstrate a successful in vitro transfer of isolated mitochondria to oocytes and provide a model for mitochondrial replacement implicated in inherited mtDNA diseases and animal cloning.

A Study on the trend of change in the number of elementary school students in Kyeonggi-do (경기도 초등학교 학생수 변화 경향에 대한 조사 연구)

  • Yoon, Yong-Gi;Choi, Ki-Seok
    • The Journal of Sustainable Design and Educational Environment Research
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    • v.20 no.1
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    • pp.43-54
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    • 2021
  • The results of the research and analysis are as follows: First, from the existing 57 types, 51 types were additionally discovered through the Gyeonggi-do research and analysis, and a total of 108 types could be subdivided. Second, as a result of the survey and analysis of Gyeonggi-do, 17 new unique types and the causes were found Third, out of 7 less than 10 years old schools, the Ns1 and Ns2 types were accounting for almost all of them. Of the 19 mid-term new schools, the Nm7 type accounted for 32.3%, and of the 41 long-term new schools, the most occupied type was Nl13 (33.5%). Among the 20 long-term rising types of 20 to 30 years old schools, the Ai19 type accounted for 22.6%, and the Ai8 type accounted for 19.4%. Among the 21 long-term decline types in existing schools, 17.0% was for the Ad5 type, 16.1% for the Ad15 type, 15.2% for the Ad10 type, 10.0% for the Ad7 type, and 8.1% for the Ad8 type.

K-Ar and $^{40}$ Ar/$^{39}$ Ar Ages from Metasediments in the Okcheon Metamorphic Belt and their Tectonic Implication (옥천 변성대 변성퇴적암의 K-Ar및 $^{40}$ Ar/$^{39}$ Ar 연대와 그 의의)

  • 김성원;오창환;이덕수;이정후
    • The Journal of the Petrological Society of Korea
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    • v.12 no.2
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    • pp.79-99
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    • 2003
  • Muscovite and biotite from 52 metasediments and 5 granites in the Hwasan area, the southwest of the Okcheon metamorphic belt and the Miwon-Jeungpyeong area, central Okcheon metamorphic belt were dated by the K-Ar and $^{40}$ Ar/$^{39}$ Ar methods. Muscovite and biotite ages from metapelitic and psammitic rocks (metasediments) of the Boeun and Pibanryeong units in the Hwasan area are concentrated in the mid-Jurassic (149-180 Ma). K-Ar and $^{40}$ Ar/$^{39}$ Ar ages for metapelitic and psammitic rocks of the Boeun and Pibanryeong units in the Miwon-Jeungpyeong area show complicated age distribution. Muscovite and biotite ages are classified by three groups, 142-194 Ma, 216-234 Ma, and 241-277 Ma. Younger (Cretaceous) ages occur only in metasediments close to Cretaceous granitic rocks in the southeastern region and the older ages of 216-277 Ma are restricted to the middle Part of the Jeungpyeong area. Most ages in the other area of the central Okcheon metamorphic belt fall between 142-194 Ma (Jurassic). K-Ar and $^{40}$ Ar/$^{39}$ Ar ages for granite from the northern part in the both the southwest and central Okcheon metamorphic belt also gave middle Jurassic ages (156-168 Ma). The similar ages from both metasediments and granites in the study areas indicate simultaneous cooling of both rocks to 300-350$^{\circ}C$ during the middle Jurassic. The state of graphitization of carbonaceous material of all metasediments in the study areas Indicates fully ordered graphite falling within a small range, from 3.353 to 3.359 ${\AA}$, which indicate amphibolite facies regional metamorphism. In the southern sector of the Boeun unit from the Hwasan area, metamorphic grade indicated by mineral paragenesis during regional intermediate-P/T metamorphism is greenschist facies. Whereas, the $d_{002}$ values for carbonaceous materials in the same sector show fully ordered graphite (ca. 500$^{\circ}C$) indicating amphibolite facies. This result with the concentration of mica ages of metasediments into the middle Jurassic, the presence of low-P/T thermal metamorphic zone (>500$^{\circ}C$) in the metasediments close to the Jurassic granite and the regional intrusion of Jurassic granites and their middle Jurassic intrusion and cooling ages may indicate the low-P/T regional thermal event during the early(\ulcorner)-middle Jurassic after main intermediate-P/T metamorphism which formed main mineral assemblage regionally in the study area. The regional thermal event failed, however, to reset the mineral assemblage of regional intermediate-P/T metamorphism except for narrow aureole (1-2 km) around Jurassic granite because e duration of thermal effect was relatively short by repid cooling of the Jurassic granite. In the middle part of the Jeungpyeong area, central Ogcheon metamorphic belt, muscovite and biotite K-Ar ages from 5 samples are 263-277 Ma and 241-249 Ma, respectively. An intermediate-P/T metamorphism is currently accepted to have occurred between 280 and 300 Ma. Therefore, the muscovite and biotite ages can be interpreted as cooling ages after Ml metamorphism indicating rapid cooling to ca 350$^{\circ}C$ between 280-300 Ma and 263-271 Ma, and biotite ages indicate slower cooling to ca. 300$^{\circ}C$ between 263-277 Ma and 241-249 Ma. However, more detail study is needed to confirm why the Permian to Triassic ages occur only in the middle Part of the Jeungpyeong area.a.

A Study of Factors Affecting the Adoption of Cloud Computing (기업의 Cloud Computing 서비스 도입의도에 영향을 미치는 Cloud Computing 특성 요인에 관한 연구)

  • Kim, Dong-Ho;Lee, Jung-Hoon;Park, Yang-Pyo
    • The Journal of Society for e-Business Studies
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    • v.17 no.1
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    • pp.111-136
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    • 2012
  • The global recession has made it more difficult for companies to invest in IT, and they are increasingly aware of the environmental costs of so doing. In these circumstances, cloud computing has emerged as a new paradigm in the business IT sector. Governments, institutes and companies around the world, as well as specifically in Korea since 2009, have turned to this model of providing IT resources. This study is concerned to identify those characteristics of cloud computing that affect its introduction on a company's part; it offers a theoretical framework describing cloud services and seeks to establish causal linkages between antecedent factors and a company's introduction and application of this form of IT provision. The features of cloud computing in particular contexts that the study selected for analysis were its scalability, speed, security, potential compatibility with existing services, efficiency, economic feasibility, dependency and credibility. The study thus related these to whether or not cloud computing was adopted, verifying adjustment effects for cloud services. On the basis of a survey of enterprise IT decision-makers, it emerged through a statistical analysis of correlations that cloud computing's efficiency, economic feasibility and credibility had an effect on its introduction. This study's results should be of use to vendors and potential purchasers of cloud computing services. It is one of the first pieces of research on cloud computing from the customer perspective, based on the perceived characteristics of cloud services as they are seen and valued by users.

Growth Characteristics and Adaptability of Introduced Achyranthes bidentata Blume in Korea (도입 우슬의 생육특성 및 국내 적응성)

  • Kim, Dong-Hwi;Sung, Jung-Sook;Kim, Myeong-Seok;Park, Chun-Geun;Park, Hee-Woon
    • Korean Journal of Medicinal Crop Science
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    • v.14 no.3
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    • pp.158-162
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    • 2006
  • Achyranthes bidentata was recorded to new medicinal crop in a revised 8th edition of the Korean pharmacopoeia. Therefore, A. bidentata began to use for same purpose with Achyranthes japonica which was cultivated since old times in Korea. This study was conducted to investigate the adaptability of A. bidentata in Korea. The germination rate and speed of A. bidentata seeds were higher than those of A. japonica in $15^{\circ}C\;and\;25^{\circ}C$. The growth of A. bidentata was greater than that of A. japonica until the 60 days after transplanting, but growth after that was the opposite. There were remarkable differences between two species in growth characteristics such as flowering date, leaf fall date, plant height, stem color and no. of branches. The flowering and leaf fall date of A. bidentata were earlier than A. japonica by July 7 and September 26. The plant height and number of branches of A.japonica were longer and more than A. bidentata, but the cluster length and no. of flowers per cluster of that were shorter and fewer than this. Number of supporting roots of A. bidentata and A. japonica was 10.7 and 14.6 per plant, respectively. The average yield was not different between two species. The yield of A. bidentata was 166 kg/l0 a in Suwon and 309 kg/10 a in Naju of Korea. The suitable cultivation region of A. bidentata was judged to southern area of Korea.

A Hybrid Genetic Algorithm for the Identical Parallel Machine Total Tardiness Problem (동종 병렬기계에서 납기지연 최소화를 위한 혼합형 유전 알고리즘의 개발)

  • Choe, Hong-Jin;Lee, Jong-Yeong;Park, Mun-Won
    • Proceedings of the Korean Operations and Management Science Society Conference
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    • 2004.05a
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    • pp.624-627
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    • 2004
  • 본 연구는 동일한 병렬기계에서의 총 납기지연의 합을 최소화하는 일정계획 문제에 대해 다룬다. 이 문제는 Lenstra et al. (1977)에 의해 NP-hard로 알려져 있으며, 작업의 수와 기계의 수가 큰 현실적 문제에 대해 적절한 시간 내에 최적해를 찾는다는 것은 사실상 불가능하다. 따라서 본 연구에서는 이 문제를 해결하기 위하여 혼합형 유전 알고리즘(hybrid genetic algorithm)을 제안한다. 혼합형 유전 알고리즘에서는 임의로 발생시킨 모집단에 대해 먼저 유전 알고리즘(genetic algorithm)이 세대를 진행하며 해를 개선한다. 유전 알고리즘이 일정기간동안 더 이상 해를 개선하지 못하면, 부분탐색 알고리즘(local-search algorithm))이 유전 알고리즘의 모집단의 개체들에 대해 해의 개선을 시도한다. 즉, 부분 탐색 알고리즘은 모집단 속의 각각의 개체를 초기해로 하여 모집단 내의 개체 수만큼의 부분 최적해(local optimum)들을 구한다. 이렇게 구한 부분 최적해들로 새로운 모집단을 구성하면 다시 유전 알고리즘이 진행된다. 이 과정을 종료조건에 이를 때까지 번갈아가며 반복 수행한다. 본 연구에서 제안한 유전 알고리즘에서는 Bean(1994)이 제안한 Random key 방법으로 개체를 표현하였으며 Park(2000)이 제안한 3가지 교차 연산자들을 채용하였다. 부분탐색 알고리즘을 위해서는 쌍대교환(pair-wise interchange) 방법을 통해 이웃해를 생성하였다. 선행실험을 통하여 제안한 혼합형 유전알고리즘에서 사용하는 다양한 모수(parameter)값들을 최적화하였으며 알고리즘의 성능을 비교하기 위하여 기존의 알고리즘과도 비교실험을 수행하였다.복적인 지표가 채택되는 경우를 포함하고 있다. 셋째는 추상적이며 측정이 어려운 지표를 채택하고 있는 경우이다. 여기에는 지표에 대한 정확한 정의가 이루어져 있지 않아 피 평가자가 불필요하거나 과다한 평가 자료를 준비해야 하거나 평가자로 하여금 평가 시 혼돈을 유발할 가능성이 있거나, 또는 상위개념의 평가항목과 하위개념의 평가항목이 혼재되어 구분이 모호한 경우를 포함하고 있다. 바탕으로 '생태적 합리성'이라는 체계적인 지식교육을 거쳐서, '환경정의' 의식의 제고로 이어가고, 굵직한 '환경갈등'의 상황에서 뚜렷한 정치적 태도와 실천을 할 수 있는 '생태적 인간상'의 육성으로 나아갈 수 있어야 한다는 것이 필자의 생각이다. 이를 위해서는 어찌되었건 체험학습 영역에서는 환경현안에 대한 사회적 실천을 '교육 소재'로 삼을 수 있어야 하며, 교과학습 영역에서는 한국사회의 환경현안에 대한 정치경제적 접근을 외면하지 말고 교과서 저작의 소재로 삼을 수 있어야 하며, 이는 '환경관리주의'와 '녹색소비'에 머물러 있는 '환경 지식교육'과 실천을 한단계 진전시키는 작업으로 이어질 것이다. 이후 10년의 환경교육은 바로 '생태적 합리성'과 '환경정의'라는 두 '화두'에 터하여 세워져야 한다.배액에서 약해를 보였으나, 25% 야자지방산의 경우 50 ${\sim}$ 100배액 어디에서도 액해를 보이지 않았다. 별도로 적용한 시험에서, 토마토의 경우에도 25% 야자지방산 비누 50 ${\sim}$ 100배액 모두 약해를 발생하지 않았으나, 오이에서는 25% 야자지방산 비누 100배액에도 약해를 나타내었다. 12. 이상의 결과, 천연지방산을 이용하여 유기농업에 허용되는 각종의 살충비누를 제조할 수 있었으

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Analysis of Consumption of Homemade Organically Processed Food Analysis of The Carbon Emission Reduction Effect from No-Tillage in Pepper (Capsicum annuum L.) Cultivation (고추의 무경운 재배에 따른 탄소저감효과 분석)

  • Lee, Gil-Zae;Choi, Yoon-Sil;Yang, Seung-Koo;Lee, Jin-Hong;Yoon, Sung-Yee
    • Korean Journal of Organic Agriculture
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    • v.20 no.4
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    • pp.503-518
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    • 2012
  • Korean type of no-tillage cultivation method which was applied on this study used the ridge and the furrow and constantly recycling them as it was suitable for Korea's weather and farming conditions. This no-tillage cultivation was reported to have little negative impact such as reduction of production (Kwon et al., 1997). In addition, it was found to have a lot of benefits as it requires less agro-materials and energy costs as well as shortened working hours because tillage operation is not needed. (Yang et al., 2012). According to an analysis, no-tillage cultivation can reduce greenhouse gas emissions by $344.7kgCO^2$ (58%) in every 10a ($1,000m^2$) compared to ordinary pepper farming technique (Korea averages). Direct-indirect reduction effects from using fertilizer and using less amount of energy were 92% and 44% respectively both of which can be considered very high. Besides the direct effects of no-tillage cultivation, soil management using no-tillage technique raises carbon sequestration effect on soil as time goes on (West & Marland, 2002), that is why the technique is expected to have constant carbon emission reduction effect. For theses reasons, distribution and expansion of Korean type no-tillage cultivation are expected to play a role as major agro-green technologies for achieving our goal of reducing greenhouse gas emissions in agricultural sector.

The Quality of Cooked Rice Prepared by Both an Electric Cooker and Electric Pressure Cooker, with Different Storage Conditions (전기 압력 밥솥 밑 전기 밥솥 취반미의 저장 조건에 따른 품질 특성)

  • Han, Gwi-Jung;Park, Hee-Joeng;Lee, Hye-Yoen;Park, Young-Hee;Cho, Yong-Sik
    • Korean journal of food and cookery science
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    • v.23 no.5
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    • pp.635-643
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    • 2007
  • This study was carried out to investigate the quality of cooked rice prepared by-an electric pressure cooker (EPC) and an electric cooker (EC) during storage at different temperatures. Four rice cultivars were used (Ilpoom, Saechucheong, Sindongjin, and Sura). The cooked rice samples were stored at room and warm temperatures for 48 hr, and at refrigerator temperatures for 10 days. Immediately after cooking, the moisture content of the EPC rice was lower than that of the EC rice; yet pH was higher for the EPC rice. The L-value of the EPC rice was reduced due to an increasing b-value. The overall acceptability of the EPC rice was high; in particular, it scored high for a roasted nutty flavor and texture. During storage, moisture content and pH decreased regardless of the cooking method. L-, a- and b-values were similar at room and refrigerator temperatures over the entire period, whereas the a-value (greenness) of the EC rice stored at warm temperature slightly decreased and the b-value (yellowness) increased after 24 hr, resulting in a decreasing L- value. Also, sensory characteristics were not significantly different for the samples stored at room temperature for 48 hr; however, at warm and refrigerator temperatures, the sensory characteristics were significantly different after 24 hr and 3 days, respectively. In conclusion, the quality of the cooked rice was effectively preserved during storage at room temperature, and qualify deterioration oaf dependent on using warming equipment as well as the rice cultivar.

Luminescence Characteristics of Blue Phosphor and Fabrication of a UV-based White LED (UV 기반 백색 LED용 청색 형광체의 발광특성 및 백색 LED 제조)

  • Jung, Hyungsik;Park, Seongwoo;Kim, Taehoon;Kim, Jongsu
    • Korean Journal of Optics and Photonics
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    • v.25 no.4
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    • pp.216-220
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    • 2014
  • We have synthesized a $CaMgSi_2O_6:Eu^{2+}$ blue phosphor via a solid-state reaction method. The $CaMgSi_2O_6:Eu^{2+}$ phosphor has monoclinic structure with a space group of C2/c (15), and an emission band peaking at 450 nm (blue) due to the $4f^7-4f^65d$ transition of the $Eu^{2+}ion$. The emission intensity at $100^{\circ}C$ is 54% of the value at room temperature. A white LED was fabricated by integrating a UV LED (400 nm) with our blue phosphor plus two commercial green and red phosphors. The white LED shows a color temperature of 3500 K with a color rendering index of 87 (x = 0.3936, y = 0.3605), and a luminous efficiency of 18 lm/W. The white LED shows a luminance maintenance of 97% after operation at 350 mA for 400 hours at $85^{\circ}C$.