• Title/Summary/Keyword: 체성분

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Antioxidant Activity and Grain Properties of Colored Rice Derived from Insertional Mutagenesis Progenies (벼 종피색 변이체에 대한 항산화 활성 분석과 미립특성)

  • Yi, Gihwan;Lee, Hyun-Suk;Sohn, Jae-Keun;Kim, Kyung-Min
    • Journal of Life Science
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    • v.22 no.12
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    • pp.1628-1636
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    • 2012
  • This study examined the antioxidant activity of the dark purple rice seeds from the rice line, MGI079, derived from insertional mutagenesis. The contents of polyphenolic compounds were 1.3 and 1.9-fold higher in the MGI079-2-1 and MGI079-2-6 rice lines than in the donor cultivar MGI079. Flavonoid contents were 6.4-fold higher in the MGI079-2-1 line. The MGI079-2-1 line showed a 24.4-fold higher activity in DPPH free radical scavenging compared to the MGI079 line. The anthocyanin content of the MGI079-2-6 line was more than 106.4-fold higher than the MGI079 line and 1.4-fold higher than the Heugnam line. Anthocyanin content in colored rice grains was negatively correlated with Hunter's L, a, and b values, with the correlation coefficients of $-5.64^{**}$, $5.21^{**}$ and -1.15, respectively. The grain length/width of a mutant of MGI079 segregated to a medium and bold type compared to the medium type of MGI079. However, the 1,000 grain weight was decreased to 13.6~19.6 g compared to 19.8 g for MGI079. Amylose content of the endosperm was 5.6~23.8% higher than in the MGI079 line. The grain of mutants of MGI079 was distinguished by its starch characteristics. The higher antioxidant activity of the MGI079-2-1 and MGI079-2-6 lines indicated functional characteristics associated with high-value resources, so future breeding should focus on the development of pigments in colored rice in new varieties.

Effect of Leaf Aqueous Extracts from Some Gymnosperm Plant on the Seed Germination, Seedling Growth and Transplant of Hibiscus syriacus Varieties (수종 나자식물의 잎 수용 추출액이 무궁화의 품종별 종자발아와 유식물 및 초기생장에 미치는 영향)

  • 배병호;김용옥
    • The Korean Journal of Ecology
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    • v.26 no.1
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    • pp.39-47
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    • 2003
  • The leaf aqueous extracts from five gymnosperms plant were investigated for allelopathy with five Hibiscus syriacus varieties. The leaf aqueous extract of Pinus rigida had the highest total phenolic compound of 2.21mg/L, whereas the soil under Pinus koraiensis canopy had the highest total phenolic compound of 1.38mg/L. Fourteen phenolic compounds were isolated from five gymnosperm plants by HPLC. Among them, phenolic compounds were the highest in P. rigida (320.56 g/mg) with the primary compound 5-sulfosalicylic acid (312.55 g/mg). The correlation between leaf total phenolic compound and pH was not significant, while the total phenolic compound of the leaf extract changed soil pH. The relative seed germination of H. syriacus varieties showed 25% was threshold concentration. The germination rates of varieties were similar to the control group or showed slight stimulation to treatment of P. koraiensis extract. H. syriacus Cambanha was similar to the control group or showed stimulation in all treated groups. H. syriacus Seohohyang showed stimulation in both root and shoot growth compared to the control group. In other varieties except Seohohyang, shoot growth was similar to the control group, while root growth was stimulated in all treated groups. The extracts of tested gymnosperms showed significantly more stimulation to transplanted Seohohyang seedlings, whereas others were similar to control or inhibited in the greenhouse. The dry weight of Seohohyang was greater in all treated groups than the control group, while other varieties were inhibited. All gymnosperm extracts stimulated the chlorophyll contents of Seohohyang and H. syriacus Koyoro but other varieties were not significantly affected. Accordingly, it is suggested that Seohohyang seems the most desirable when planted within these five gymnosperms.

Behaviors of Arsenic in Paddy Soils and Effects of Absorbed Arsenic on Physiological and Ecological Characteristics of Rice Plant lll. Effect of Water Management on As Uptake and the Growth of Rice Plant at As Added Soil (토양중(土壤中) 비소(砒素)의 행동(行動)과 수도(水稻)의 비소흡수(砒素吸收)에 의(依)한 피해생리(被害生理) 생태(生態)에 관(關)한 연구(硏究);Ⅲ.물관리(管理)가 수도의 비소흡수(砒素吸收) 및 생육(生育)에 미치는 영향(影響))

  • Lee, Min-Hyo;Lim, Soo-Kill-H
    • Korean Journal of Environmental Agriculture
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    • v.6 no.1
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    • pp.1-6
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    • 1987
  • A pot experiment was conducted to find out the effect of water management on the growth and uptake of arsenic and inorganic nutrients of rice plant at As added soil. The arsenic were added to soil at the levels of As 0, 10, 50, 100 and 150 ppm, respectively. Water management was done with two ways: intermittent irrigation from ten days after transplanting, and continuous submersion until harvest. Higher soil As levels increased As content in plant but reduced growth rate. Aresenic content in plant was considerably reduced with intermittent irrigation compared to continuous submersion. Rice growth showed also same trend. With increasing As levels in soil, N content in plant was increased but P, K, Ca, Mg, $SiO_2$, Fe and Mn content in plant were tend to be decreased. These inorganic nutrients in plant were also much absorbed in continuous submersion compared to intermittent irrigation. Soil pH was slightly increased with increasing As levels in soil while soil Eh has no relationship with soil As levels. On the other hand, soil pH was higher in the treatment of continuous submersion than that of intermittent irrigation but soil Eh showed reverse trend. With increasing As levels in soil, water soluble-As and Ca-As fractions in soil tend to be increased with continuous submersion, but these fractions has no tendency with intermittant irrigation.

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Mathematical Transformation Influencing Accuracy of Near Infrared Spectroscopy (NIRS) Calibrations for the Prediction of Chemical Composition and Fermentation Parameters in Corn Silage (수 처리 방법이 근적외선분광법을 이용한 옥수수 사일리지의 화학적 조성분 및 발효품질의 예측 정확성에 미치는 영향)

  • Park, Hyung-Soo;Kim, Ji-Hye;Choi, Ki-Choon;Kim, Hyeon-Seop
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.1
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    • pp.50-57
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    • 2016
  • This study was conducted to determine the effect of mathematical transformation on near infrared spectroscopy (NIRS) calibrations for the prediction of chemical composition and fermentation parameters in corn silage. Corn silage samples (n=407) were collected from cattle farms and feed companies in Korea between 2014 and 2015. Samples of silage were scanned at 1 nm intervals over the wavelength range of 680~2,500 nm. The optical data were recorded as log 1/Reflectance (log 1/R) and scanned in intact fresh condition. The spectral data were regressed against a range of chemical parameters using partial least squares (PLS) multivariate analysis in conjunction with several spectral math treatments to reduce the effect of extraneous noise. The optimum calibrations were selected based on the highest coefficients of determination in cross validation ($R^2{_{cv}}$) and the lowest standard error of cross validation (SECV). Results of this study revealed that the NIRS method could be used to predict chemical constituents accurately (correlation coefficient of cross validation, $R^2{_{cv}}$, ranging from 0.77 to 0.91). The best mathematical treatment for moisture and crude protein (CP) was first-order derivatives (1, 16, 16, and 1, 4, 4), whereas the best mathematical treatment for neutral detergent fiber (NDF) and acid detergent fiber (ADF) was 2, 16, 16. The calibration models for fermentation parameters had lower predictive accuracy than chemical constituents. However, pH and lactic acids were predicted with considerable accuracy ($R^2{_{cv}}$ 0.74 to 0.77). The best mathematical treatment for them was 1, 8, 8 and 2, 16, 16, respectively. Results of this experiment demonstrate that it is possible to use NIRS method to predict the chemical composition and fermentation quality of fresh corn silages as a routine analysis method for feeding value evaluation to give advice to farmers.

The Changes of Hardness and Microstructure of Dongchimi according to different kinds of water (물의 종류를 달리한 동치미의 경도 변화 및 세포벽 관찰)

  • 심영현;안기정;김지은
    • Korean journal of food and cookery science
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    • v.20 no.1
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    • pp.86-94
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    • 2004
  • The purpose of this study was to investigate the changes of hardness and microstructure of Dongchimi cooked with various source of water(distilled water, purified water, Cho Jung Carbonated Natural water). This study was conducted to observe the changes of pH, total acidity, salt content, turbidity, texture and microstructure. Dongchimi cooked with source of water of water was fermented at 10$^{\circ}C$ for 46 days. The changes of pH on Dongchimi cooked with various source of water decreased in all samples during fermentation period, and then showed a slowly decrease after 12 days of fermentation. The total acidity of Dongchimi cooked with Cho Jung Carbonated Natural water was arrived slowly at best tasting condition 0.3 ∼ 0.4 point compared with other conditions. So Dongchimi cooked with Cho Jung Carbonated Natural water was continued to the best tasting condition for end of fermentation. At early stage of fermentation, the changes of turbidity of Dongchimi used Cho Jung Carbonated Natural water showed highly as compared with other test condition for 12th days of fermentation. The maximum cutting force of chinese radish of Dongchimi showed the highest value among all at the 25th day of ripening and then decreased gradually. The maximum cutting force of chinese radish of Dongchimi used Cho Jung Carbonated Natural water was the highest compared with other conditions at 25th day of fermentation. The calcium content of Dongchimi juice used Cho Jung Carbonated Natural water was observed hish at the early stage of fermentation and showed the highest value at 25th day of ripening. The calcium content of chinese radish and Dongchimi juice of Dongchimi cooked with water purifier was lower than that of Dongchimi cooked with Cho Jung Carbonated Natural water, and was higher than that of Dongchimi cooked with Distilled water at the early stage of fermentation. The magnesium content in all samples increased gradually from the early stage of fermentation. The microstructure showed disintegration appearance of middle lamella and cell wall during fermentation period.

MICROLEAKAGE OF THE EXPERIMENTAL COMPOSITE RESIN WITH THREE COMPONENT PHOTOINITIATOR SYSTEMS (3종 광중합개시제를 함유한 실험용 복합레진의 미세누출도)

  • Kim, Ji-Hoon;Shin, Dong-Hoon
    • Restorative Dentistry and Endodontics
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    • v.34 no.4
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    • pp.333-339
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    • 2009
  • This study was done to determine if there is any difference in microleakage between experimental composite resins, in which various proportions of three component photoinitiators (Camphoroquinone, OPPI, Amine) were included. Four kinds of experimental composite resin were made by mixing 3.2% silanated barium glass (78 wt.%, average size; 1 ${\mu}m$) with each monomer system including variously proportioned photoinitiator systems used for photoinitiating BisGMA/BisEMA/TEGDMA monomer blend (37.5:37.5:25 wt.%). The weight percentage of each component were as follows (in sequence Camphoroquinone, OPPI, Amine): Group A - 0.5%, 0%, 1% / Group B - 2%, 0.2%, 2% / Group C - 0.2%, 1%, 0.2% / Group D - 1%, 1%, 2%. Each composite resin was used as a filling material for round class V cavities (diameter: 2/3 of mesiodistal width; depth: 1.5 mm) made on extracted human premolars and they were polymerized using curing light unit (XL 2500, 3M ESPE) for 40 s with an intensity of 600 mW/$cm^2$. Teeth were thermocycled fivehundred times between $50^{\circ}C$and $550^{\circ}C$for 30s at each temperature. Electrical conductivity (${\mu}A$) was recorded two times (just after thermocycling and after three-month storage in saline solution) by electrochemical method. Microleakage scores of each group according to evaluation time were as follows [Group: at first record / at second record; unit (${\mu}A$)]: A: 3.80 (0.69) / 13.22 (4.48), B: 3.42 (1.33) / 18.84 (5.53), C: 4.18 (2.55) / 28.08 (7.75), D: 4.12 (1.86) / 7.41 (3.41). Just after thermocycling, there was no difference in microleakage between groups, however, group C showed the largest score after three-month storage. Although there seems to be no difference in microleakage between groups just after thermocycling, composite resin with highly concentrated initiation system or classical design (Camphoroquinone and Amine system) would be more desirable for minimizing microleakage after three-month storage.

Prelaunch Study of Validation for the Geostationary Ocean Color Imager (GOCI) (정지궤도 해색탑재체(GOCI) 자료 검정을 위한 사전연구)

  • Ryu, Joo-Hyung;Moon, Jeong-Eon;Son, Young-Baek;Cho, Seong-Ick;Min, Jee-Eun;Yang, Chan-Su;Ahn, Yu-Hwan;Shim, Jae-Seol
    • Korean Journal of Remote Sensing
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    • v.26 no.2
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    • pp.251-262
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    • 2010
  • In order to provide quantitative control of the standard products of Geostationary Ocean Color Imager (GOCI), on-board radiometric correction, atmospheric correction, and bio-optical algorithm are obtained continuously by comprehensive and consistent calibration and validation procedures. The calibration/validation for radiometric, atmospheric, and bio-optical data of GOCI uses temperature, salinity, ocean optics, fluorescence, and turbidity data sets from buoy and platform systems, and periodic oceanic environmental data. For calibration and validation of GOCI, we compared radiometric data between in-situ measurement and HyperSAS data installed in the Ieodo ocean research station, and between HyperSAS and SeaWiFS radiance. HyperSAS data were slightly different in in-situ radiance and irradiance, but they did not have spectral shift in absorption bands. Although all radiance bands measured between HyperSAS and SeaWiFS had an average 25% error, the 11% absolute error was relatively lower when atmospheric correction bands were omitted. This error is related to the SeaWiFS standard atmospheric correction process. We have to consider and improve this error rate for calibration and validation of GOCI. A reference target site around Dokdo Island was used for studying calibration and validation of GOCI. In-situ ocean- and bio-optical data were collected during August and October, 2009. Reflectance spectra around Dokdo Island showed optical characteristic of Case-1 Water. Absorption spectra of chlorophyll, suspended matter, and dissolved organic matter also showed their spectral characteristics. MODIS Aqua-derived chlorophyll-a concentration was well correlated with in-situ fluorometer value, which installed in Dokdo buoy. As we strive to solv the problems of radiometric, atmospheric, and bio-optical correction, it is important to be able to progress and improve the future quality of calibration and validation of GOCI.

Effect of Reduced Fertilization Considering Residual Soil Nutrients on Rice Yield and Salt Removal in Greenhouse Vegetables and Rice Cropping System (토양 잔존 양분을 고려한 시설채소 후작 벼의 감비 재배에 따른 벼 수량과 토양 염류 제거 효과)

  • Jeon, Weon-Tai;Park, Hyang-Mi;Chung, Joung-Bae;Park, Ki-Do;Park, Chang-Young;Yang, Won-Ha
    • Korean Journal of Soil Science and Fertilizer
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    • v.38 no.2
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    • pp.85-91
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    • 2005
  • Nutrients are built up in paddy soils after greenhouse vegetable cultivations with relatively high rates of chemical fertilizers and composts during winter season, and the continuous nutrient accumulation is problematic in crop cultivation. Rice cultivation after greenhouse vegetables is one option for removing the accumulated nutrients in the soils. The object of this experiment was to examine the effect of reduced fertilization to rice on the removal of accumulated soil nutrients and rice yield in greenhouse vegetables and rice cropping system. Experiments were carried out at Changwon and Uiryeong in Gyeongnam province in 2001. The cropping systems were watermelon-rice and pumpkin-watermelon-watermelon-rice in Changwon and Uiryeong, respectively. The soils were Gangseo series (coarse loamy, mixed, nonacid, mesic family of Aquic Fluventic Eutrochrepts) at Changwon and Hampyeong series (fine loamy, mixed, mesic family of Fluvaquentic Dystrochrepts) at Uiryeong. Treatments of conventional fertilization ($N-P_2O_5-K_2O=11-4.5-5.7$, $kg\;10a^{-1}$), no basal fertilization, no top dressing, and no fertilization were included in the experiments. Plant growth and total nitrogen content in the plant were greater as the amount of fertilizer applied were increased. Whereas $SiO_2/T-N$ rate in rice plant and nitrogen use efficiency were greater as the amount of fertilizer applied were reduced. Rice yields were not significantly different among the treatments of conventional, no top-dressed, and no-basal fertilization in Uiryeong, and the rice yields were significantly also not different between the treatments of conventional and no top-dressed in Changwon. The removal of salts in soils after rice cultivation was the highest at the treatment of no-basal fertilization in both of the sites. Therefore, reduced fertilization for rice cultivation after greenhouse vegetables could remove salts accumulated in paddy soils without any significant reducing of rice yield.

The Optimization and Verification of an Analytical Method for Sodium Iron Chlorophyllin in Foods Using HPLC and LC/MS (식품 중 철클로로필린나트륨의 HPLC 및 LC/MS 최적 분석법과 타당성 검증)

  • Chong, Hee Sun;Park, Yeong Ju;Kim, Eun Gyeom;Park, Yea Lim;Kim, Jin Mi;Yamaguchi, Tokutaro;Lee, Chan;Suh, Hee-Jae
    • Journal of Food Hygiene and Safety
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    • v.34 no.2
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    • pp.148-157
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    • 2019
  • An optimized analytical method for sodium iron chloriphyllin in foods was established and verified by using high performance liquid chromatography with attached diode array detection. An Inertsil ODS-2 column and methanol-water (80:20 containing 1% acetate) as a mobile phase were employed. The limit of detection and quantitation of sodium iron chloriphyllin were 0.1 and 0.3 mg/kg, respectively, and the linearity of calibration curve was excellent ($R^2=0.9999$). The accuracy and precision were 93.9~104.95% and 2.0~7.7% in both inter-day and intra-day tests. Recoveries for candy and salad dressing were ranged between 93 and 104% (relative standard deviation, (RSD) 0.3~4.3%), and between 83 and 115% (RSD 1.2~2.0%), respectively. Liquid chromatography mass spectrometry was used to verify the main components of sodium iron chlorophyllin which were Fe-isochlorin e4 and Fe-chlorin e4.

The Structual Restoration on Gyeongju-Style Piled Stone-Type Wooden Chamber Tombs (경주식 적석목곽묘의 구조복원 재고)

  • Gweon, Yong Dae
    • Korean Journal of Heritage: History & Science
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    • v.42 no.3
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    • pp.66-87
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    • 2009
  • The definition of the structure of wooden chamber tomb(piled stone-type) is as follows. It is a tomb with wooden chamber, and stones were piled on top of the wooden chamber, and then a wooden structure was placed on top of the piled stones, and more stones were piled on top of the wooden structure, and sealed with clay. Of course this definition can vary according to periods, the buried, etc. Gyeongju-style piled stone type wooden chamber tombs have some distinguished characteristics compared to general definition of piled stone type wooden chamber tombs. Outside the wooden chamber, either stone embankments or filled-in stones were layed out, and pilet-in stones are positioned right above the wooden chamber, and almost every class used this type, and finally, it is exclusively found in Gyeongju area. First generations of this Gyeongju-style piled stone type wooden chamber tombs appeared in first half of 5th century. These tombs inherited characteristics like ground plan, wooden chamber, double chamber(inner chamber and outer chamber), piled stones, burial of the living with the dead, piled stones, from precedent wooden chamber tombs. However these tombs have explicit new characteristics which are not found in the precedent wooden chamber tombs such as stone embankments, wooden pillars, piled stones(above ground level), soil tumuluses. stone embankments and wooden pillars are exclusively found on great piled stone type above-ground level wooden chamber tombs such as the Hwangnamdaechong(皇南大塚). Stone embankments, wooden pillars, piled stones(above ground level) are all elements of building process of soil tumuluses. stone embankments support outer wall of above-ground level wooden chambers and disperse the weight of tumuluses. Wooden pillars functioned as auxiliary supports with wooden structures to prevent the collapse of stone embankments. Piled stones are consists of stones of regular size, placed on the wooden structure. And after the piled stones were sealed with clay, tumulus was built with soil. Piled stones are unique characteristics which reflects the environment of Gyeongju area. Piled stone type wooden chamber tombs are located on the vast and plain river basin of Hyeongsan river(兄山江). Which makes vast source of sands and pebbles. Therefore, tumulus of these tombs contains large amount of sands and are prone to collapse if soil tumulus was built directly on the wooden structure. Consequently, to maintain external shape of the tumulus and to prevent collapse of inner structure, piled stones and clay-sealing was made. In this way, they can prevent total collapse of the tombs even if the tumulus was washed away. The soil tumulus is a characteristic which emerges when a nation or political entity reaches certain growing stage. It can be said that after birth of a nation, growing stage follows and social structure will change, and a newly emerged ruling class starts building new tombs, instead of precedent wooden chamber tombs. In this process, soil tumulus was built and the size and structure of the tombs differ according to the ruling class. Ground plan, stone embankments, number of the persons buried alive with the dead, quantity and quality of artifacts reflect social status of the ruling class. In conclusion, Gyeongju-style piled stone type wooden chamber tombs emerged with different characteristics from the precedent wooden chamber tombs when Shilla reached growing stage.