• Proceedings of the Plant Resources Society of Korea Conference
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• 2012.05a
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• pp.3-3
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• 2012

자원식물은 인간의 천연물에 대한 관심 고조와 미래 생물자원으로서의 경제적 가치가 재인식되어 미국을 비롯한 선진국은 오래전부터 유용한 식물자원 보존과 이를 이용한 산업화에 정부가 앞장서 추진하고 있다. 더욱이 우리나라는 국토면적에 비해 많은 식물종이 분포하고 뚜렷한 4계절과 다양한 토양특성으로 우수한 기능성식물자원이 많으며 이들 식물자원의 이용과 효능에 대한 풍부한 지식이 전통적으로 이어져 왔다. 그럼에도 불구하고 우수한 자원식물에 대한 보존과 이용이 과학화 체계화되지 못하여 세계적인 품종이나 제품개발은 미흡한 실정이다. 그동안 우리나라는 토종자원에 대한 관심과 꾸준한 연구개발로 농촌진흥청 보존 농업유전자원은 세계 6위 수준('10년말 291천점) 이며, 멀티기능 유용물질 함유 작물 개발 및 실용화 기술수준도 기술력 지수 세계7위로 높게 평가되고 있다. 그러나 이들 자원식물로부터 유용물질을 이용하여 세계시장을 선도하는 천연물신약, 건강기능성식품, 한방화장품, 천연 색소 및 향료 등의 제품개발은 미흡하여 앞으로 유망 자원식물을 이용한 체계적이고 과학적인 고부가 산업 육성을 위한 집중적인 투자가 필요한 시점이다. 자원식물을 이용한 천연물 신약개발은 고부가가치 제품과 산업재산권 확보가 가능하므로 수입 대체와 기술 및 제품수출이 충분히 이루어질 수 있다고 보여 현재 연구개발 중인 신약후보 도출 및 임상개발 과제의 수요를 반영한 후 단계별 대규모 신약개발 노력이 필요하다 하겠다. 건강기능 식품 개발은 고령사회 진입과 노령인구 증가로 노화억제 및 고령자 건강유지용 식품비중이 지속적으로 높아질 것으로 추정되어 약용식물을 소재로 개인 맞춤형 나노바이오 건강기능성 식품 개발을 통한 다양한 제품개발이 요구된다. 또한 우리나라 화장품분야의 전반적인 기술수준은 세계최고 기술의 62% 수준이나 화장품 소재의 자원식물 발굴과 나노기술 접목을 통한 효능의 극대화는 소비자의 웰빙 트랜드와 외국화장품과의 차별화 전략에 맞물려 국내 한방화장품의 세계화가 가능한 품목 중 하나이다. 식용 및 염색용 색소도 자원식물이 풍부하고 천연색소 시장이 크게 확장되고 있어 자원식물 중 경제성과 기술적 개발 가능성을 고려하여 단계적인 개발이 요구된다. 특히, 우리의 식물자원, 전통지식, 과학기술이 통합되어 효능을 현저하게 상승시키는 천연물신약, 건강보조식품, 화장품 등이 개발된다면 세계시장을 선도할 날도 멀지 않을 것으로 기대한다.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.1
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• pp.37-47
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• 2019

Recently, due to the increase in skin diseases caused by particulate matter, endocrine disruptor and environmental changes, the trend of development of cosmetic materials has been shifting to the more safe and effective ingredients based on natural materials rather than existing synthetic compounds like steroids and antihistamines. This study aimed to develop a new natural cosmetic materials using oriental herbs such as Eucommiae cortex, Alpinia oxyphylla Miquel and Bombyx batryticatus. First, DPPH assay was performed to examine the antioxidative activity of the herbal extract (Mix) and 98.8% DPPH radical scavenging activity was confirmed at $400{\mu}g/mL$ concentration of it. In order to confirm the whitening efficacy of oriental herbal extracts(mix), the amount of melanin synthesized after stimulation of ${\alpha}-MSH$ with B16F10 cells was measured. Results showed that it was decreased to 27.1% comparing with the only ${\alpha}-MSH$ treated group, which confirmed the whitening efficacy. Also, both nitric oxide(NO) production and iNOS and COX-2 expression were significantly reduced in RAW264.7 macrophages activated by LPS in the presence of the extracts(Mix). The mRNA expression of the inflammatory cytokines such as $IL-1{\alpha}$, $IL-1{\beta}$, IL-6, and $TNF-{\alpha}$ was also analyzed to confirm the inhibition effect of the extracts on inflammation. Finally, to confirm the enhancement of skin barrier function, the expression of claudin 1 gene, a tight junction protein, was observed using human keratinocyte HaCaT cells and increased as concentration dependent manner. From these results, it is concluded that the oriental herbal extracts(Mix) containing Eucommiae cortex, Alpinia oxyphylla Miquel and Bombyx batryticatus is effective for antioxidant, anti-inflammation, skin whitening, and skin barrier and thus could be applied as a new natural cosmetic material.

• Journal of the Korean Applied Science and Technology
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• v.38 no.6
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• pp.1568-1576
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• 2021

Here, we conducted the study on the possibility of using FK-13, a short analog of human-derived antibacterial peptide LL-37, as a cosmetic preservative to discover a natural cosmetic preservative that is safe for human body. For the purpose, FK-13 composed of 13 amino acids was synthesized by solid-phase peptide synthesis, and purified using reversed phase-high performance liquid chromatography (RP-HPLC). The purity and molecular weight were confirmed by liquid chromatography-mass spectrometry (LC-MS) analysis. FK-13 showed high antimicrobial activity on the three gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, and Staphylococcus epidermidis), the three gram-negative bacteria (Escherichia coli, Salmonella typhimurium, and Pseudomonas aeruginosa), and also even the fungus Candida glabrata. FK-13 had a broad spectrum of antibacterial activity, showing a suitability as a cosmetic preservative. In addition, FK-13 showed high thermostability and higher antibacterial activity in a comparative test with existing natural herbal cosmetic and chemical preservatives. Therefore, as FK-13 is a safe material and has high antibacterial activity at a low concentration, it is likely to be applied as a peptide natural cosmetic preservative that can replace existing chemical preservatives.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.26-27
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• 2018

With entry into force of the Nagoya Protocol to promote the fair sharing of the benefits of accessing and utilizing genetic resources, much support has been given to research on the development of biomaterials and products using domestic natural resources. Conservation and resource-saving of native species became very important through Nagoya Protocol enactment. The trend of cosmetic industry has been shifing from use synthetic chemicals to natural biomaterials, due to the safety regulations on new materials, ban on animal experiments, and expansion of cosmeceuticals range. In addition, functional cosmetic range has been expanded from whitening, wrinkle improvement, and ultraviolet shielding, to hair loss, hair loss alleviation, acne relaxation, and moisturizing of atopic skin, thus causing the activation of research about field of efficacy evaluation on natural biomaterials and commercialization. Chungbuk province is fostering the bio industry as a key industry for regional economic growth. For this purpose, Osong Biotechnology Complex/Ochang Science Industrial Complex in middle area, Jecheon biovalley in northern region, and Chungju Enterprise city have been established, thus playing a pivotal role in Bio innovative cluster in Korea. In particular, it was established the osong cosmetics clinical research support center to develop the cosmetics industry in chungbuk, thereby supporting clinical trials, efficacy evaluations, overseas certification, and overseas market entry in order to advance into the global market. In addition, oriental plants such as astragalus propinquus, schisandra chinensis, eucommia, alpiniae oxyphyllae fructus and biancaea sappan are being actively studied as global cosmetic ingredients through the promotion of various national research and development projects using natural materials in chungbuk province. The chungbuk natural product industry is expected to grow further throughout cosmetics industry development in the future, as companies and research institutes are actively promoting the secure index of effective material in natural products and effective material commercialization.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2011.10a
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• pp.8-8
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• 2011

인류는 자연으로부터 식량과 질병 치료를 위하여 자연스럽게 약초를 얻을 수 있었다. 약 6만 년 전 네안데르탈인의 무덤에서 오늘날 향료로 쓰이는 약초가 발견 될 정도로 인류가 약초를 이용한 역사는 오래 되었다. 약초는 난치병, 노화, 성인병 등 현대의학에서 미비점을 보완하는 대체의약 산업의 원료이자 새로운 생물자원의 보고로서, 그 중요성이 커지고 있다. 새로운 생물자원 소재로 약초는 약용성분을 함유한 식물자원의 확보 및 개발 등 고부가산업의 소재로 선진국에서 연구개발의 경쟁이 치열하며, 약초산업은 천연소재 신약과 기능성 식품, 화장품, 생활용품 등으로 확대되고 있다. 의약품 분야에서는 새로운 시대의 중요한 생명산업으로서 약초는 한약뿐 아니라 신약 개발을 위한 소재로도 부각되고 있으며, 항암제인 택솔, 신종플루 치료제인 타미플루 등 천연물 약의 가능성은 이미 증명되었다. 식품 분야에서는 재배기술 발달과 웰빙 트렌드 속에서 약초는 신선채소와 기능성 식품으로 변신하고 있다. 한약재의 이미지를 벗어난 약초는 음료와 과자, 술 등으로 활용되고, 비빔밥 등 다양한 약선음식으로도 개발되고 있다. 산업소재의 활용분야에서는 약초는 특히 화장품 산업의 소재로 각광받고 있다. 이미 다양한 화장품 회사에서 한방화장품을 출시하여 세계 속으로 진출하고 있다. 또한 약초는 천연 색소이자 생활 용품의 소재로 활용되고 있는데, 약초를 활용한 염색약과 여성 생리대, 샴푸 등의 인기가 높다. 약초산업의 부가가치 증대를 위하여 한약재 위주의 약초산업을 천연물 신약, 기능성 식품, 화장품, 산업 소재 등 고부가가치 시장으로 확장시키고, 우리 약초 산업의 세계화를 추진해야 한다. 연구개발 투자 확대와 빠르게 변화하는 시장을 반영하여 연구 방향을 수립하고, 산학연 및 생산자단체 등과 새로운 약초 산업의 블루오션 창출을 위해 협력을 해야 할 것이다. 또한 약초의 생산기술 안정화를 통해 산업화 기반을 구축하여, 약초 공급의 안정화, 약초 생산 농가 소득 안정화가 필요하며, 마지막으로 변해가는 약초 산업의 트렌드에 부응할 수 있는 품질관리와 유통 질서 확립을 위한 정책과 제도의 선진화가 뒷받침 되어야 할 것이다.

• Journal of the Korean Chemical Society
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• v.54 no.6
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• pp.707-710
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• 2010

Mt. Jiri, where is located in southwestern area of Korea, can be a treasury of herbal medicine plants grown wild. More than 1200 species currently classified as herbs are grown or cultivated. Recently, the studies on the safflower which attracts the interests in the inhibition of skin aging in the modern people have been actively investigated, and this study aims to confirm that polyphenol compound, the main ingredient of safflower, can be a natural material to cause the delayed effect on the skin aging, inhibiting the generation of wrinkle in the 30 to 40s middle aged females. Primarily, this study is to confirm the anti-oxidizing effect by DPPH assay in vitro, and to evaluate the efficacy of inhibition or improvement on the wrinkle generation in the face skin by clinical trials, additionally. In addition, we are confident to contribute on the improvement of functional cosmetic industry, developing the sample product of anti-aging mask pack which has the main ingredient with the extract of safflower.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.2 s.51
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• pp.153-159
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• 2005

In this study, we investigated the application of an extract from Ephedra sinica which has been composed of traditional Korean medicine as a whitening ingredient. The extract of Ephedra sinica which was obtained from the mixture of methanol and water (1:1) the inhibitory effect of tyrosinase. Then, Ephedra sinica was extracted by two different solvents. One was water and the other was methylene chloride. Only, the water extract of Ephedra sinica showed the inhibitory effort of tyrosinase; the anti-tyrosinase activity with $0.2\%$ of the water extract was $60.6\%$. But the extract of Ephedra sinica in methylene chloride fraction showed little inhibitory effect on tyrosinase. The inhibitory effect of the concentrated water extract of Ephedra sinica was tested on L-DOPA auto-oxidation and melanin synthesis in B-16 melanoma. In L-DOPA auto-oxidation, $0.5\%$ of the concentrated water extract showed $87\%$ of inhibition of L-DOPA auto-oxidation and the $0.75\%$ concentrated Ephedra sinica extract in wafer fraction inhibited $98.8\%$ of that. In melanin synthesis of B-16 melanoma, the concentrated water effect of Ephedra sinica inhibited $70.2\%$ or $79.9\%$ of inhibitory effect on that at the concentration of $0.05\%$ or $0.075\%$, respectively. For verifying the skin whitening effect of the concentrated water extract of Ephedra sinica in vivo, we performed the clinical test of that. The research showed the significant skin whitening effect of a cream containing $0.5\%$ Ephedra sinica extract and the statistical analysis showed a significant difference (p < 0.05) between sample (containing $0.5\%$ Ephedra sinica extract) and placebo after 10 weeks.

• KSBB Journal
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• v.22 no.5
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• pp.271-277
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• 2007

Natural pigments have many applications like colouring agent, pigments, food additives, and antiseptics. At present, instead of synthetic pigments that have contributed to the development of industry, many kinds of natural pigments have been developed. The constituents of gardenia fruits, Gardenia jasminoides ELLIS, are traditionally known as herb medicine and natural dyes/pigments due to the customer is needs. The fruits produce yellow carotenoid pigments and iridoid compounds. The two main components in the yellow pigments are called crocin and crocetin. The extraction mode of yellow pigment from Gardenia is depended upon the extraction time, temperature, and volume of solvent. Red pigments or blue pigments formed from geniposide and amino acids have been reported a lot. Geniposide, the principal iridoid glucoside contained in gardenia fruit, was hydrolyzed to genipinic acid or genipin as a precursor for the pigment by enzymatic or chemical reaction. These red or blue pigments prepared with materials hydrolyzed of geniposide and amino acid and had properties governed by the electrostatic character of the amino acid. The pigments showed good stability to heat and pH but were gradually bleached by light while the other natural pigments are unstable in light, heat, acid, and base solution. The safety of the pigments was considered to be of little virulences in comparison to synthetic pigments.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.33 no.2
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• pp.79-86
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• 2007

The aim of this study was to investigate the inhibitory effects of Forsythia suspensa fruit extracts (FSfE) ($0.1{\sim}1.0%$) and their active component on melanogenesis. FSfE dose-dependently inhibited melanin synthesis (up to $63.1{\pm}3.1%$ at the concentration of 1.0%) without cell cytotoxicity. We purifed one active compound from FSfE and identified its structure. It was identified as 4-[(3,4-Dimethoxyphenyl)methyl)]dihydro-3-[(4-hydroxy-3-methoxyphenyl)methyl]-2(3H)-furanone (arctigenin) by $^1H-NMR,\;^{13}C-NMR$, and Mass analysis. Arctigenin also inhibited melanin synthesis in a dose dependent manner (up to $52.7{\pm}3.1%$ at the concentration of $3.0{\mu}g/mL$). In order to verify the whitening activity of the cream containing 3% FSfE, we performed the clinical test with twenty five female volunteers for 8 weeks. Bioengineering analyses and visual assessment by doctors at the initial time point and 8 weeks after applications showed that cream containing FSfE have significant whiteining effect (paired t-test). From the results, we conclude that the FSfE can be used as a useful whitening agent.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.4 s.54
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• pp.305-310
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• 2005

To obtain effective and safe depigmenting agents, we investigated the effects of Scirpi rhizoma, a medicine among Chinese herbs, on melanogenesis. Dried S. rhizoma was refluxed with 70% aqueous ethanol and the extract was evaporated to dryness. To determine the effects as a whitening agent, various in vitro tests were performed such as free radical scavenging activity, melanin formation assay, tyrosinase activity and expression of tyrosinase, TRP-1 and TRP-2(western blot and RT-PCR) in B16 melanoma cells. S. rhizoma showed scavenging activities of free radicals and reactive oxygen species (ROS) with the $IC_{50}\;of\;638{\mu}g/mL$ against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and $21.7{\mu}g/mL$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. S. rhizoma significantly inhibited melanin production in B16 melanoma cells. S. rhizoma treatment(48 h) suppressed the biosynthesis of melanin up to 27% at 100{\mu}g/mL$ and reduced tyrosinase activity up to 31% at $100{\mu}g/mL$ in B16 melanoma cells. S. rhizoma was also able to significantly inhibit tyrosinase and TRP-1 expression in protein and mRNA level. These results suggest that S. rhizoma inhibited melanin biosynthesis by regulating tyrosinase activity and expression in B16 melanoma cells. Therefore, S. rhizoma may be useful as a new antioxidant and whitening agent to inhibit melanogenesis.