• The Korean Journal of Ecology
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• v.22 no.1
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• pp.59-63
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• 1999

To verify allelopathic effects, seed germination and seedling growth test, chemical analysis and bioassay of selected species were carried out with naturally occurring chemicals of Artemisia capillaris. Seed germination ratio of Calamagrostis arundinacea. Youngia denticulata and Lactuca indica var. laciniata showed decrease in proportion to increase in aqueous extracts concentration of A. capillaris. while that of Cosmos bipinnatus and Leonurus sibiricus did not. However, dry weight growth of selected species treated with the same extracts as the above experiment was inhibited remarkably compared to the germination test. In the test at different concentrations of essential oil from A. capillaris, seedling growth of A. princeps var. orientalis and Plantago asiatica was suppressed according to the concentration of the essential oil, and root growth of the selected species was more inhibitory than that of shoot growth. Thirty-six chemical compounds were identified from A. capillaris plant by gas chromatography. Seven compounds out of 36 were bioassayed, and terpinen-4-ol was the most toxic among the tested substances.

• Journal of Convergence for Information Technology
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• v.10 no.7
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• pp.232-239
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• 2020

This study evaluated the antioxidant and anti-inflammatory activity of ethanol extracts using three parts of the chives plant: the bulb, the leaf, and the flower. As a result of DPPH and ABTS radical scavenging ability, the scavenging activity of the flower extract was higher than that of the bulb and leaf. In addition, as a result of FRAP analysis, antioxidant activity increased in all extracts depending on the extract concentration. The total polyphenol content was high in the following order: flower (11.29±0.37 mgGAE/g) > leaf (6.61±0.14 mgGAE/g) > bulb (5.7±0.67 mgGAE/g) extract. The cytotoxicity of the three extracts against rat macrophage RAW264.7 cells and HaCaT cells, both of which are human cutaneous keratinocyte cell lines, was minimal. NO by LPS was generated as a result of examining the anti-inflammatory activity of each extract through the NO colorimetric analysis method and ELISA. TNF-α secretion was decreased to a significant level in the flower ethanol extract. Therefore, these results indicate that there is a high possibility that the ethanol extract of chives, a natural plant resource, can be used as a cosmetic raw material.

• The Korean Journal of Community Living Science
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• v.16 no.4
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• pp.95-102
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• 2005

The present study was performed to investigate the 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging and Proly1 endopeptidase (PEP) inhibitory activities of plant extracts. The whole extract of Fragaria yezoensis inhibited the DPPH radical by $90.4\%$ and the stem of Gingko biloba, Gardenia jasminoides for. grandiflora and Rhododendron yedoensa var. poukhanene, the loaves of Rhododendron mucronulatum var. ciliatum, Gardenia jasminoides for. grandiflora and Corylus sieboldiana var. mandshurice, the fruit of Cornus officinalis, and the root of Gingko biloba showed high DPPH radical scavenging activities. In the case of PEP inhibitory activities, high inhibition was observed in the whole Plant of Fragaria ananassa, Fragaria yezoensis and Hypericum erectum, the stem of Actinidia arguta and Rhododendron yedoensa var. poukhanese, the leaves of Rhododendron yedoensa var. poukhanense and Rosa davurica, the fruit of Cornus officinalis. and the root of Acer okamotoanum. There was significant correlation (P=0.000) between DPPH radical scavenging and prolyl endopeptidase inhibitory activities, thus some of plant extracts such as whole Fragaria yezoensis, fruit of Cornus officinalis had high activities in both DPPH-scavenging and prolyl endopeptidase inhibition. Therefore, it is required to examine the mechanical interaction between DPPH-scavenging and prolyl endopeptidase inhibitory activities and further studying plant extracts with both these activities is desired to develop agents for preventing and treating of Alzheimer's disease.

• Journal of Practical Agriculture & Fisheries Research
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• v.25 no.1
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• pp.20-35
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• 2023

Chemical preservatives have a good effect on antibacterial activity, but many side effects on the human body have been reported. Recently, the development of natural preservatives that are harmless to the human body and have preservative functions and self-efficacy is active. In addition, in order to increase the absorption rate of natural products by the human body, the method of fermentation using strains is also increasing. Therefore, this study selected varieties that are harmless to the human body and have good antibacterial activity. 1. The yield of origin, thickness and solvent was investigated. Scutellaria baicalensis Georgi was made in China and received a yield of 21.88% from 50% ethyl alcohol extract. Salvia miltiorrhiza Bunge was made in Korea and received a yield of 25.62% from 50% ethyl alcohol extract. Dryopteris crassirhizoma Nakai was made in China and received a yield of 6.50% from 70% ethyl alcohol extract. 2. The solid fermentation with the S. baicalensis and S. miltiorrhiza with B. Subtilis yield gained 24.40%, 39.30%, and D. crassirhizoma obtained 11.10% yield when fermented with L. casei. 3. After the liquid fermentation, a clear zone of 9mm was identified for the S. aureus strain in the S. baicalensis, and the antibacterial activity was not confirmed in S. miltiorrhiza and D. crassirhizoma. 4. When the S. baicalensis was fermented with L. Casei, it showed high antibacterial activity in C. albicans and S. aureus. S. miltiorrhiza showed antibacterial activity in S. aureus when it was solid with S. cerevisiae. When the spectators were solid with L. casei and S. cerevisiae, antibacterial activity was high in E. coli and S. aureus. Overall, the antibacterial activity after fermentation was much higher than when fermented. 5. The change in active ingredients was baicalin 101.57, baicalein 28.26, and wogonin 5.33mg/g in the S. baicalensis that did not ferment solid. When solid fermentation with S. cerevisiae, the content of baicalinin with baicalin 94.31, baicalein 30.41, and wogonin 3.57mg/g was found to have increased. S. miltiorrhiza that was not fermented, salvianolic acid A was 1.82mg/g, and when fermented with S. cerevisiae, it increased to 5.70mg/g. The active ingredients of the spectators were flavaspidic acid AP, flavaspidic acid PB, flavaspidic acid AB, and flavaspidic acid BB.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.102-102
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• 2018

중국유(中國柚)의 주요성분은 비타민 C가 레몬보다 10배나 많이 함유되어 있고 또한 과피 안쪽에 펙틴 성분이 다량으로 함유되어 있다. 특히 과피가 일반 감귤류 보다 10배 이상 두꺼워 비타민P의 ?량 또한 다량으로 함유되어 있다. 즉, 유기산, 비타민P와 비타민C가 매우 많이 함유되어 있어서 예로부터 중국에서는 감기와, 피부노화, 피로를 방지하는데 사용되어져 왔으며 이러한 효능은 유기산에서도 많이 발생되었다. 그 밖에 비타민B군과 당질, 단백질등이 다른 감귤류 품종보다 많고, 모세혈관을 보호하는 헤스페리딘(비타민P)이 다량으로 함유되어 있어서 심혈관 질환 장애와 뇌졸증, 뇌출혈 등을 억제하는 효과를 가지고 있다. 또한 몸 안에 쌓여 있는 노폐물 등을 밖으로 배출시켜주는 효과가 양호하여 피부미용에 효과가 있는 것으로 알려져 있다. 본 실험에서는 중국유(中國柚)의 항산화활성과 총 폴리페놀 함량을 조사하였던 바, 과육보다 과피에서 폴리페놀 함량이 높게 나타났으며 항산화 활성도 과피에서 매우 양호한 결과를 나타내었다. 중국유(中國柚)의 품종별로 보면 과피가 두껍고 과육이 붉은색을 띈 품종에서 항산화 효과가 더 양호한 반응을 나타내었다. 이러한 결과는 과육에 함유되어있는 안토시아닌의 함량의 차이인 것으로 생각된다. 특히 과피가 과육의 추출과정에서 에탄올보다 메탄올 추출물에서 더 양호한 결과를 보였다. 이러한 결과들로 미루어볼 때 중국유(中國柚)의 항산화 활성은 천연화장품의 신소재로서의 가치를 가지고 있다고 판단된다.

• Journal of Life Science
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• v.31 no.11
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• pp.1037-1045
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• 2021

The prevalence of obesity is increasing worldwide, and since obesity is associated with dietary factors and sedentary lifestyles, it is a disease that is readily developing in the modern population. Because obesity is accompanied by serious complications such as diabetes and cardiovascular disease, prevention and treatment are important. Currently, drugs such as liraglutide and phentermine are used to treat obesity by suppressing appetite and inducing gastrointestinal motility delay. However, various side effects may occur, including thyroid cancer, cardiovascular problems, and central nervous system disorders. Therefore, to explore an obesity treatment method with relatively few side effects, a method known as "fat browning" was introduced to change white adipose tissue into brown adipose tissue to increase energy consumption. Ongoing studies are attempting to find effective natural substances to safely induce browning. Many natural substances have been identified. The induction of browning by treatment with natural substances generally involves three mechanisms: positive control of browning-inducing factors, inhibition of differentiation into white adipose tissue, and the activation of mechanisms related to browning. In this study, we describe plant extracts with known browning-inducing effects, such as strawberry, black raspberry, cinnamomum cassia, and Ecklonia stolonifera extracts. We also summarize the underlying mechanisms of action identified thus far, including the signaling pathway mediated by these extracts to induce browning. Furthermore, the effects of brown adipose tissue generated through browning on heart disease as an endocrine organ disruptor are discussed.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.263-263
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• 2022

물봉선속(Impatiens L.)은 일년생 또는 다년생 초본으로 우리나라에 5-7종이 분포한다. 물봉선의 화장품용 항산화제 및 천연 방부제로서의 유용성이 밝혀졌으며, 최근 항염, 항산화 등 생리활성에 대한 연구가 국내 자생하는 물봉선 및 노랑물봉선을 대상으로 수행된 바 있으나 이하 분류군에 대한 연구는 미비한 실정이다. 본 연구에서는 국내 자생하는 물봉선속 분류군들의 항산화활성과 생리 활성물질의 함량을 분석하고 지역 간 분류군별 차이를 확인하고자 하였다. 따라서 국내 자생하는 물봉선속 분류군들의 항산화활성을 검정하기 위하여 DPPH, ABTS, TPC, TFC 4가지 방법을 이용하여 분석하였다. 국내 자생하는 물봉선속 5분류군 가야물봉선(Impatiens atrosanguinea (Nakai) B.U.Oh & Y.P.Hong), 노랑물봉선(Impatiens noli-tangere L), 물봉선(Impatiens textorii Miq), 미색물봉선(Impatiens noli-tangere var. pallescens Nakai), 처진물봉선(Impatiens furcillata Hemsl)이 12개 지역에서 수집되었으며, 잎 추출물(70% 에탄올)에 대해 분석되었다. 물봉선속 분류군들의 잎 추출물의 DPPH 라디칼 소거 활성 검정 결과 가야물봉선(4.91 ± 3.00 mgAAE/g)이 가장 높았고 처진물봉선(1.77 ± 0.55 mgAAE/g)이 가장 낮았으며, ABTS의 경우 가야물봉선(3.14 ± 1.35 mgAAE/g)로 가장 높았고 미색물봉선(1.87 ± 0.16 mgAAE/g)이 가장 낮았다. TPC의 경우 미색물봉선(5.48 ± 1.05 ugGAE)이 가장 높았고 노랑물봉선(2.78 ± 1.98 ugGAE)이 가장 낮았으며, TFC의 경우 물봉선(0.70 ± 0.20 ugGAE/g)이 가장 높았고 노랑물봉선(0.45 ± 0.08 ugGAE/g)이 가장 낮게 나타났다. 수집지역별로는 각각 DPPH와 ABTS의 경우 노랑물봉선, TPC의 경우 가야물봉선, 노랑물봉선, 물봉선, TFC의 경우 처진물봉선이 지역별 차이를 보였다. 이번 연구 결과를 토대로 국내 자생하는 물봉선속 분류군 별 항산화활성과 생리활성물질 차이를 확인할 수 있었고 추후 유용 소재로써의 이용과 우수 개체선발에 도움이 될 것으로 사료 된다.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.303-311
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• 2018

In this study, a preliminary evaluation of the antioxidant and anti-inflammatory activity of the Ficus erecta var. sieboldii (Miq.) King (FES) leaf extract has been performed to assess its potential as a natural resource for food and medicinal materials. FES was extracted using 70% EtOH and then fractionated sequentially using n-hexane, $CH_2Cl_2$, EtOAc, and n-BuOH. To screen for antioxidant and anti-inflammatory agents effectively, the inhibitory effect of the FES extracts on the production of oxidant stresses (DPPH, xanthine oxidase, and superoxide) and pro-inflammatory factors (NO, iNOS, COX-2, $PGE_2$, IL-6, and $IL-1{\beta}$) in the murine macrophage cell line RAW 264.7 activated with lipopolysaccharide (LPS) was examined. Among the sequential solvent fractions of FES, the $CH_2Cl_2$ and EtOAc fractions showed decreased production of oxidant stresses (DPPH, xanthine oxidase and superoxide), and the hexane and $CH_2Cl_2$ fractions of FES inhibited the production of pro-inflammatory factors (NO, iNOS, COX-2, and $PGE_2$). The $CH_2Cl_2$ fraction also inhibited the production of pro-inflammatory cytokines ($TNF-{\alpha}$, IL-6, and $IL-1{\beta}$). These results suggest that FES has a significant effects on the production of oxidant stresses and pro-inflammatory factors and may be used a natural resource for antioxidant and anti-inflammatory agents.

• Korean Journal of Environmental Agriculture
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• v.16 no.4
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• pp.347-355
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• 1997

For the selection of plants contained pesticidal active conpounds, 31 families 59 species of native plants were collected and biological activites of their crude extracts against brown planthopper (Nilaparvata lugens), Xanthomonas oryzae pv. oryzae and allelopathy were examined. Among the screened plants, the crude extracts from the leaves of Ricinus communis and Sophora angustifolia showed 100% and 82% of mortality on brown planthopper at the concentration of 1% (w/v) respectively. Mixed crude extracts of Sophora angustifolia root and Melia azedarach seed exhibited 128${\sim}$155% of synergistic effects on the mortality of brown planthopper. In case of fungicidal activity, the crude extracts from the leaves of 8 plants including Chrysanthemum indicum were inhibitory of the growth of Xanthomonas oryzae pv. oryzae at 1%(w/v) soluction. Seed germination of radish (Raphanus sativas) and barnyardgrass (Echinochloa crus-galli) was inhibited by 0.5%(w/v) leaf extracts of Pinus densiflora and Quercus acutissima. MeOH extracts of Pinus denislora leaves gave 100% inhibition in seed germintion of radish at 2%(w/v) soluction and showed a complete inhibition in seedling growth of barnyardgrass as well as radish at 5% solution.

• Journal of Applied Biological Chemistry
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• v.59 no.1
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• pp.13-17
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• 2016

As natural plant-based industry has been expanded, the use of oriental medicinal plants as cosmeceutical material received a lot of attentions in the cosmetic industry. Among various medicinal plants, Potentilla chinensis have drawn interests for its biological effects. Although several attempts were tried to study its biological effect as medicinal plant, only limited results were reported to evaluate its biological effect as cosmeceutical material. In this study, we examined the possibility of root extract from Potentilla chinensis as a cosmeceutical material because the root part has been reported to have several kinds of health promoting effects. After extraction of roots, biological evaluation including anti-inflammation, anti-wrinkle, whitening effect and moisturizing effect was performed. As a result, the root extract showed remarkable biological activities through stimulating synthesis of elastin and aquaporin-3, and inhibiting melanin synthesis, cyclooxygenase-2 expression and expression of metallopeptidase-1.