• Korean Journal of Medicinal Crop Science
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• v.14 no.1
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• pp.49-55
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• 2006

Antioxidative and antibacterial activities of endemic plants extracts in Korea were investigated. Hydrogen radical scavenging activity was 99.72% in an elm tree, 99.725 in a Job's tears, 99.575 in an eggplant, and 94.025 in a barrenwort. Antibacterial activity of ethanol extract from 23 different species of wild plants were determined. The gull nut was showed the strongest antibacterial activities (16.0-19.0 mm) and also showed high antibacterial activities in a pine neddles, a gulmyungja, a wild ginger plants, a ginko (9.5-11.5 mm). Only extracts of Saurrranceae extract showed the antibacterial activity in Bacillus subtilus, Stapylococcus aureus, Escherichia coli, Samonella entetotidis. An antibacterial activities were showed weakly in an esungcho and an eggplant. A ground ivy showed Antibacterial activity on Stapylococcus aureus. A barren wort showed in antibacterial activity on Stapylococcus aureus, and E. coli. This results were suggested that many edemic plants resources contains antioxidative and antibacterial substances.

• Korean Journal of Plant Resources
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• v.31 no.2
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• pp.109-116
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• 2018

Erectile dysfunction (ED), also known as impotence, is the inability to attain and sustain an erection firm enough to have sexual intercourse. Frequent ED may be a symptom of health problems including heart disease, obesity, alcoholism, stress, smoking, and depression, that need treatment. This study aimed to effect of complex extract (CPL) including Cornus officinalis on sexual function factor in the erectile dysfunction rat model. The erectile dysfuction rat model was induced by cimetidine (500 mg/kg in 5% ethanol, oral injection 2 weeks). Rats were oral administered with different concentration of CPL in rat erectile dysfunction model. As a results, sexual function factors (NO, cGMP) significantly improved in CPL treated groups (CPL-300, 600, 900 mg/kg) compared to CON group. Serum testosterone was increased in a dose-dependent manner after CPL treatment. Furthermore, administrations of CPL restored lumen areas of the prostate in the erectile dysfunction rat model. These results indicated that CPL alleviated erectile dysfunction by increasing sexual function factor and testosterone in rat model. CPL could be used to natural treatement for erectile dysfunction. However, further study is required to identify active ingredient and its mechanism of erectile dysfunction.

• Korean Journal of Food Science and Technology
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• v.32 no.3
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• pp.736-741
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• 2000

The abilities of petroleum ether-extracts prepared from 75 plants to inhibit tyrosinase activity were evaluated in reverse micelles composed of isooctane/AOT(100 mM)/phosphate buffer(20 mM, pH 8.0) containing tyrosinase(105.3 units/mL) and 3,4-dihydroxyphenylalanine(0.18 mM). Compared with control which has no plant extracts, garlic could completely inhibit in vitro melanogenesis by tyrosinase, and Chinese quince, sweet potato, onion, radish bud and apple did more than 60%. Lipophilic extracts of medicinal plants and herbs such as rosemary, coriander, cinnamomi ramulus, crataegii fructus, ramulus biotae folium, mume fructus, menthae herba, eucommiae cortex and clove also inhibited tyrosinase activity more than 60%. When the extraction yield of lipophilic materials was considered together with their inhibition effect on tyrosinase, it was possible to select plants of which tyrosinase inhibitors could be produced in high quantity from unit weight. Using reverse micelles, the analysis of the capacity of lipophilic materials to inhibit tyrosinase activity which was difficult up to present could be possible.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2012.03a
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• pp.69-69
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• 2012

지구상에는 약 40만 종의 식물이 존재하는 것으로 알려져 있으며 이 중에 는 질병의 치료를 위한 약재로 이용되거나 나무는 대부분 목재 등으로 이용된다. 향나무(Juniperus chinenesis L.)는 항균성 및 방충성이 뛰어난 상록수로서 목재는 조각재나 가구재로 사용되고 민간 및 한의학에서 다양한 증상의 약재로 쓰이고 있으며 부패한 냄새를 제거할 목적이나 향재 및 향료로도 쓰이고 있다. 열매의 정유성분은 화장품, 술, 캔디 등에 사용되며 cedrol은 향료보류제, 유분은 훈향료, 목부는 고혈압, 곽란, 심복통, 통기파혈에 쓰인다. 향나무는 폴레페놀 성분이 함유되어 있고 피톤치드가 많이 나오는 것으로 알려져 있으며 심재의 폴리페놀 화합물은 항암, 항균, 항알러지, 노화방지 및 심장질환 등을 예방하거나 지연시키는 등 광범위한 약리학적 활성을 나타내어 식품, 의약품, 화장품, 향료등 다양한 분야에 활용되고 있다. 향나무 추출물에 관한 연구로는 성분분석과 생리활성 등에 관한 의약학 분야의 다양한 연구와 항산화, 항균활성 등 약리작용에 연구가 최근 다양하게 이루어지고 있는 것을 볼 수 있다. 측백나무속에 속하는 나무들은 대부분 정유 성분을 함유하고 있는 특성으로 항균성 등 바이오 기능성 소재 개발을 위한 연구가 활발히 진행되고 있으나, 향나무 추출물의 염색성에 관한 연구는 찾아보기 어려운 실정이다. 이에 본 연구에서는 다양한 용매를 이용하여 향나무의 잎, 열매, 수피, 심재 각 부위 추출물을 이용하여 견직물에 대한 염색성을 살펴봄으로써 향나무 추출물을 활용한 천연염색과 염재로서의 가능성을 확인하고자 하였다. 실험 결과 증류수를 염액 용매로 염색한 결과 모든 추출물의 잎, 열매 염색포의 색상은 대부분 옅은 Y계열로 나타났으며 향나무의 수피와 심재 염색포는 대부분 적색기미가 강한 YR계열로 나타났다. 에탄올 혼합액을 염액 용매로 하여 염색한 결과 향나무의 잎과 열매의 색상은 대부분 Y계열의 색상을 나타냈고 유기용매 추출염색포에서 정유성분으로 추정되는 물질 때문에 균염이 어려웠으며 수피와 심재 염색포에서 대부분 R계열의 색상이 뚜렷이 나타났다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.103-103
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• 2020

눈개승마 (Aruncus dioicus)는 장미과(Rosaceae) 눈개승마속(Aruncus)에 속하는 다년생 초본식물로 울릉도에서 자생하는 잎줄기 채소이다. 독특한 향기가 특징이며, 인삼맛, 두릅맛, 고기맛이 나는 산채로 세가지 맛이 난다고 하여 예로부터 삼나물로도 불러왔다. 눈개승마에는 caffeic acid, 1-O-Caffeoyl-β-D-glucopyranose 등의 항산화 성분이 다량 함유되어 있으며, 전초는 해독, 편도선염에 효과가 있어 약용으로 쓰이고 어린순은 탄수화물과 무기물 함량이 풍부하다. 비지는 대두를 이용하여 두부를 제조하는 과정에서 발생되는 것으로서 대두로부터 수용성 단백질이 추출되었지만 나머지 약 23%의 단백질, 57%의 식이섬유와 이소플라본 등의 유용한 성분을 다량 포함하고 있다. 또한 비지의 단백질은 다른 식품에는 부족한 황 함유 아미노산과 리신의 함량이 비교적 많고, 두부에 거의 없는 식이섬유가 다량 함유된 고 식이섬유 소재이다. 그리하여, 이러한 콩비지를 고부가가치 식품 소재로 활용하기 위해 건조 미세분말 비지를 제조하고, 이를 산채와의 혼합 가공을 통한 기능증진 효과를 연구하였다. 일반적으로 산채는 쓴맛 제거와 보관성 증진을 위해 데침공정을 주로 하나 산채를 물에 데칠 경우 산채의 유용성분이 대부분 손실된다. 눈개승마의 경우 유용성분이 17.25%, 그 중 폴리페놀 성분이 약 90% 손실된다. 본 연구에서는 기존의 데침공정 대신 찜공정을 이용하여 산채의 유용성분을 보존하고 콩비지를 첨가하여 산채 유용성분의 생이용성 증진 효과를 연구하였다. 눈개승마를 콩비지와 혼합하여 고온고압 가공함으로써 산채의 쓴맛을 줄이고 보관성을 높이는 동시에 눈개승마 폴리페놀의 단량체화를 통해 눈개승마 단독가공에 비하여, 단량체 비율이 약 100~200배 증가하는 것을 확인하였다. 본 연구를 통하여 콩비지의 고부가가치 소재로의 가능성을 확인하였을 뿐만 아니라 산채의 다이어트·건강기능식품 소재로의 산업화에도 기여할 수 있을 것으로 기대된다.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.3
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• pp.307-317
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• 2019

To develop sustainable new natural anti-aging ingredients from Korean native plants, we investigated the cultivation potential of Nymphoides indica using the eco-friendly aquaponics system, and tested the anti-aging effects from N. indica extracts. N. indica could be grown in aquaponics system using floating leaved deep water culture method, and propagated through rhizome propagation. It was confirmed that the nitrate ($80{\mu}g/mL$), potassium ($63.5{\mu}g/mL$) and water temperature ($25^{\circ}C$) greatly affected the cultivation of the N. indica. In addition, synergistic effects were found when two major components (3,7-di-O-methylquercetin-4'-O-${\beta}$-glucoside & sweroside) were present at more than about $5{\mu}g/mL$. The extract had a significant effect on the recovery of skin cells damaged by environmental pollutant such as $benzo[{\alpha}]pyrene$, ammonium nitrate, formaldehyde. It also suppressed $PGE_2$, $TNF-{\alpha}$ and COX-2, and inhibited the production of MMP-1. Taken together, the results suggested that the standardized extracts of N. indica cultivated in the aquaponics has considerable potential as a new cosmetics ingredient with an anti-aging effect.

• Food Science and Preservation
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• v.24 no.8
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• pp.1113-1121
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• 2017

The purpose of this study was to determine antioxidant and physiological activities of water and 70% ethanol extracts from Ligularia fischeri by extraction methods. The yield of water and ethanol extracts from Ligularia fischeri was 15.23% and 17.45%, respectively. The polyphenol and flavonoid contents of ethanol extracts of Ligularia fischeri (LEE) were $17.17{\pm}4.38mg/g$, $35.06{\pm}6.69mg/g$, respectively. The electron donating ability and SOD like activity, and ABTS radical ability of all Ligularia fischeri extracts were increased in a dose dependent manner, and those was the highest in LEE. Nitrite scavenging ability was higher in pH 1.2 than that in pH 3.0, and ethanol extract showed higher ability in pH 1.2 and 3.0. The xanthine oxidase and inhibition effect of all Ligularia fischeri extracts on tyrosinase were dose-dependently increased, and those was the highest in ethanol extracts of Ligularia fischeri. Reducing power was 1.2 at extract concentration $1,000{\mu}g/mL$ in water and ethanol extracts of Ligularia fischeri and the highest in water extract of Ligularia fischeri at concentration of $62.5-500{\mu}g/mL$. These results may contribute to development of processed food and health functional food with Ligularia fischeri.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.297-300
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• 2002

Dental caries suppression effect of herb extracts were measured in comparison with sucrose. The result of susceptibility test for dental caries by using Streptococcus mutans KCTC 3065, Streptococcus cricetus KCTC 3292, Streptococcu rattus KCTC 3294, Actinomyces naeslundii KCTC 9013 indicated that Astragalus membranaceus BUNGE was effective than another herbs.

• Journal of Life Science
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• v.22 no.6
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• pp.788-793
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• 2012

High concentration of antibiotics has been used to treat the outbreak of edwardsiellosis caused by Edwardsiella tarda in aquaculture. However, not all of the bacteria have been killed with high concentrations of antibiotics treatment by the formation of persister cells with a dormant state. The main objective of this study was to kill persister cell using antibiotics with the addition of natural plant compounds. Antibiotics used in this study consist of 100 mg/ml florfenicol and 100 mg/ml amoxicillin. Ten natural plant compounds with persister cell inhibitor activity to E. coli were obtained from Protein Engineering and Systems Biology Lab. of Sungkyunkwan University. The persister cell inhibition activities of those natural plant compounds were evaluated in test tube. Concentrations of the antibiotics were in the ranges of 25~200 ${\mu}g/ml$. The persister cell formation was observed after 16 hours of culture. Persister cells were killed by antibiotics with natural plant compounds. Among ten natural plant compounds, Gynostemma pentaphyllum, Mallotus japonicus, and Orixa japonica showed persister cell formation inhibition activities. The optimal concentrations of G. pentaphyllum, M. japonicus, and O. japonica for the inhibitor of persister cell formation were 100 ${\mu}g/ml$, 100 ${\mu}g/ml$, and 200 ${\mu}g/ml$, respectively. In vivo study was carried out to evaluate the effect of the antibiotics with natural plant compounds using aquacultural fish, olive flounder, as test animals. G. pentaphyllum, M. japonicus, and O. japonica of 30 ${\mu}g/ml$, 10 ${\mu}g/ml$, and 10 ${\mu}g/ml$ with antibiotics reduced cumulative mortalities, showing the effectiveness of persister cell inhibition.

• Journal of agriculture & life science
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• v.45 no.6
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• pp.213-226
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• 2011

This study was performed to investigate the effect of essential oils and ethanolic extracts of approximately 650 plant species on acetylcholine esterase (AChE) enzyme activity using Ellman's colorimetric method in 96-well microplates. The results showed that the ethanolic extracts from twig of Sophora subprostrata, twig of Phellodendron amurense, seed of Corylopsis coreana, and essential oil (EO) from Citrus paradisi, Cupressus sempervirens, Ocimum basilicum, Pinus sylvestris and Rosmarinus officinalis inhibited more than 80% of AChE activity. Among these, EO from Pinus sylvestris, C. sempervirens and C paradisi exhibited higher values of AChE inhibitory activity, which were 75, 84 and 99% at a concentration of 50 ug/ml, respectively. Finally, EO from C paradisi (grapefruit, GEO) showed the highest inhibitory activity towards AChE, which showed 91% of inhibition at a concentration of 20 ug/ml. We also examined the anti-dementia effects of GEO in mouse by passive avoidance test and Morris water maze test. The model mouse (male, ICR) of dementia (negative control) was induced by administration of scopolamine (1 mg/kg body weight). The latency time of sample group administrated with GEO (100 mg/kg, p.o.) increased significantly as compared with negative control on passive avoidance test. There were significant recovery from the scopolamine-induced deficits on learning and memory in water maze test through daily administrations with GEO (100 mg/kg, p.o.). From these results, we conclude that GEO treatment might enhance the cognitive function, suggesting that the EO of C. paradis may be a potential candidate for improvement of perceptive ability and dementia.