• Applied Biological Chemistry
• /
• v.43 no.4
• /
• pp.247-252
• /
• 2000

As functionality investigation of Korean traditional soybean fermentation foods, an antihypertensive peptide was separated during Chunggugjang fermentation by Bacillus subtilis CH-1023 and investigated inhibitory effect against angiotensin converting enzyme. After incubation at $20^{\circ}C,\;30^{\circ}C,\;40^{\circ}C,\;50^{\circ}C,\;60^{\circ}C$ for the $0{\sim}72$ hrs, protein content, protease activity and angiotensin converting enzyme inhibitory rate were determined. The protein content and protease activity were increased and reached maximum at 60 hrs fermentation with $40^{\circ}C$ and decreased after the 60 hrs fermentation. The optimum condition for antihypertensive peptide from Chunggugjang was appeared for 60 hrs at $40^{\circ}C$. Crude extract of Chunggugjang was partially purified by Amicon YM-3 membrane filtration and Sephadex G-10, G-25 gel filtration. The purified peptide showed inhibitory rate of 94.3% with 0.5 mg peptide content. The most prominent amino acid composition of the peptide from Chunggugjang was alanine, followed by phenylalanine, histidine.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.29 no.4
• /
• pp.737-742
• /
• 2000

As functionality investigation of a soybean fermentation food, a angiotensin converting enzyme inhibitory peptide was separated during natto fermentation by Bacillus natto and inhibitory effect was investigated. After incubation at each 2$0^{\circ}C$, 3$0^{\circ}C$, 4$0^{\circ}C$, 5$0^{\circ}C$, 6$0^{\circ}C$ for the 0~72 hr, protein content, protease activity and angiotensin converting enzyme inhibition were determined. The protein content and protease activity were increased and reached maximum at 60 hr fermentation with 4$0^{\circ}C$ and decreased after the 60 hr fermentation during natto fermentation. The optimum condition for angiotensin converting enzyme inhibitors was appeared at fermentation for 60 hr at 4$0^{\circ}C$. Crude extract of natto was partially purified by Amicon membrane YM-3 and Sephadex G-10, G-25 gel filtration, stepwise. The inhibitory rate was increased in a concentration dependent manner, espcially the most potent activity about 74.74% at 1.0 mg peptide content. The most prominent amino acid of the peptide from natto was alanine, followed by phenylalnine, histidine.

• Applied Biological Chemistry
• /
• v.35 no.1
• /
• pp.23-29
• /
• 1992

Bromelain was purified from Korean pineapple, Ananas comosus, L. The enzyme was purified about 21 fold by DEAF-cellulose ion-exchange chromatography and gel filtration on Sephadex G-150. Purified enzyme was confirmed as active single band by polyacrylamide electrophoresis and the molecular weight was estimated to be about 22,000 by SDS-PAGE. The optimum pH and temperature were 6.0 and $60^{\circ}C$, respectively. The range of its stability to the pH and temperature were respectively 5.0 to 7.0 and below $50^{\circ}C$. It was found that $Mn^{2+}$ increased the enzyme activity, whereas $Mg^{2+}\;and\;Fe^{2+}$ decreased it abruptly. The purified enzyme was inhibited by p-chloromercuribenzoic acid, indicating that reactive SH groups are required for the enzyme activity. The reaction of the enzyme followed typical Michaelis-Menten kinetics with Km value of $5.747{\times}10^{-4}\;M\;and\;Vmax\;of\;131.58\;{\mu}g/min$ for casein. When meat was treated with the enzyme, free soluble nitrogen and amino acid nitrogen increased as enzyme concentration increased.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.47 no.4
• /
• pp.79-89
• /
• 2010

Recently, distributed video coding (DVC) has been actively studied for low complexity video encoder. The complexity of the encoder in DVC is much simpler than that of traditional video coding schemes such as H.264/AVC, but the complexity of the decoder in DVC increases. In this paper, we propose the Region-Of-Interest (ROI) based DVC with low decoding complexity. The proposed scheme uses the ROI, the region the motion of objects is quickly moving as the input of the Wyner-Ziv (WZ) encoder instead of the whole WZ frame. In this case, the complexity of encoder and decoder is reduced, and the bite rate decreases. Experimental results show that the proposed scheme obtain 0.95 dB as the maximum PSNR gain in Hall Monitor sequence and 1.87 dB in Salesman sequence. Moreover, the complexity of encoder and decoder in the proposed scheme is significantly reduced by 73.7% and 63.3% over the traditional DVC scheme, respectively. In addition, we employ the layered belief propagation (LBP) algorithm whose decoding convergence speed is 1.73 times faster than belief propagation algorithm as the Low-Density Parity-Check (LDPC) decoder for low decoding complexity.

• Applied Biological Chemistry
• /
• v.47 no.1
• /
• pp.55-60
• /
• 2004

Changes of cell wall components and softening enzyme during the preparation of persimmon pickles with soy sauce and soy paste were investigated. The contents of alcohol insoluble substance and cell wall extracted from persimmon pickles in soy sauce and soy paste were gradually decreased to the 20th day of storage and then decreased rapidly, but the contents of water soluble material extracted from persimmon pickles in soy sauce and soy paste was increased during the storage time $(0{\sim}50\;days)$. The contents of lignin, pectin and acid-soluble hemicellulose of persimmon pickles in soy sauce and soy paste were decreased during the storage, but contents of alkali-soluble hemicellulose was increased. The contents of cellulose almost did not change during storage of pickles. The hardness of persimmon pickles in soy sauce and soy paste was gradually increased up to the 30th day of storage and then decreased. The activities of polygalacturonase and pectinesterase as softening enzyme in persimmon pickles with soy sauce and soy paste increased during storage. And ${\beta}-galactosidase$ activity was slightly increased to the 30th day of storage and after increased rapidly.

• Korean Journal of Food Science and Technology
• /
• v.20 no.1
• /
• pp.63-71
• /
• 1988

Total lipid contents of tomato fruits were 97.6mg% and composed of neutral lipid(45.1mg%), phospholipid(31.2mg%) and glycolipid(12.4mg%). The contents of neutral lipid were slowly increased from the period of climacteric rise, but those of glycolipid and phospholipid were slowly decreased at the end of storage period. Major fatty acids in all lipids were identified to be palmitic, stearic, linoleic and oleic acids. The contents of linoleic acid in all lipids at $25^{\circ}C$ and those in neutrallipid at $15^{\circ}C$ were decreased, while those in phospholipid were slightly increased during storage. The contents of palmitic acid in neutral lipid were decreased, whereas those in glycolipid and phospholipid have a tendency to increase during storage. As for normal atmospheric pressure-normal temperature(NAP-N) condition, volatiles from homogenated tomato fruits were positively correlated with palmitic acid of neutral lipid, whereas negatively correlated with linoleic acid. As for subatmospheric pressure-low temperature(SAP-L) condition, the relationship between volatiles and fatty acids of neutral lipid was similar to NAP-N condition. Volatiles were positively correlated with linoleic acid of glycolipid and stearic acid of phosholipid, whereas negatively correlated with oleic acid of glycolipid and palmitic acid of phospholipid, respectively.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2005.05b
• /
• pp.806-810
• /
• 2005

홍수로 인한 국민의 재산과 인명피해를 방지 또는 최소화하기 위한 방법으로 주요지점을 선정하여 홍수예보를 수행하고 있다. 이러한 홍수예보는 초기 강우법, 수위법 등의 단순한 방법으로 수행되었으나, 컴퓨터가 발달되면서 여러 형태의 홍수유출모형이 개발되어 현재에는 홍수유출모형을 이용한 홍수예보를 수행하고 있다. 낙동강홍수예보 시스템은 1987년 낙동강홍수통제소의 개소시 42개소의 수위관측소와 54개소의 우량관측소를 중심으로 43개 소유역을 구분하고 티센망을 구성하여 홍수예보시스템을 구축하였다. 홍수예보시스템이 구축된 후 여러 차례의 매개변수 개선과정을 거쳐 운용하고 있다. 반면, 지속적인 수문관측소의 증설이 이루어졌으나 이를 모형에 반영하지 않아 증설된 수위관측소를 반영하여 114개 소유역으로 구분하고 추가된 우량관측소를 이용하여 티센망을 구성하였으며, 당초 반영되지 않았던 소규모댐을 모형에 추가하여 예측의 정확도를 재고하였다. 증설된 수문관측소를 모형에 반영과 함께 우량자료의 신뢰성 및 안정성을 확보하기 위한 우량관측소의 확장방안을 제시하였다. 본 연구에서 수행한 낙동강홍수예보 시스템의 소유역을 114개로 나누고 기존의 유효우량산정법, 유역유출모형 하도유출모형의 매개변수를 전통적인 방법으로 재산정하여 홍수기에 적용한 결과 당초의 홍수예보시스템으로 예측한 결과보다 소유역의 적절하게 재구성하는 것으로도 예측의 정밀도가 개선되었음을 확인할 수 있었다. 그러나, 소유역의 수와 하도의 수가 증가하면서 발생하는 오차를 감소시킬 필요성이 있으며, 이는 지속적인 시스템의 운용을 통하여 모형구성을 개선함으로서 어느 정도 개선될 것으로 판단된다. 그밖에 기존의 매개변수산정을 전통적인 방법에서 우리나라 하천 또는 낙동강유역의 하천에 적당한 방법을 개발하여 개선시킬 필요성이 있다.>$4.3\%$로 가장 근접한 결과를 나타내었으며, 총 유출량에서도 각각 $7.8\%,\;13.2\%$의 오차율을 가지는 것으로 분석되어 타 모형에 비해 실유량과의 차가 가장 적은 것으로 모의되었다. 향후 도시유출을 모의하는 데 가장 근사한 유출량을 산정할 수 있는 근거가 될 것이며, 도시재해 저감대책을 수립하는데 기여할 수 있을 것이라 판단된다.로 판단되는 대안들을 제시하는 예비타당성(Prefeasibility) 계획을 수립하였다. 이렇게 제시된 계획은 향후 과학적인 분석(세부평가방법)을 통해 대안을 평가하고 구체적인 타당성(feasibility) 계획을 수립하는데 토대가 될 것이다.{0.11R(mm)}(r^2=0.69)$로 나타났다. 이는 토양의 투수특성에 따라 강우량 증가에 비례하여 점증하는 침투수와 구분되는 현상이었다. 경사와 토양이 같은 조건에서 나지의 경우 역시 $Ro_{B10}(mm)=20.3e^{0.08R(mm)(r^2=0.84)$로 지수적으로 증가하는 경향을 나타내었다. 유거수량은 토성별로 양토를 1.0으로 기준할 때 사양토가 0.86으로 가장 작았고, 식양토 1.09, 식토 1.15로 평가되어 침투수에 비해 토성별 차이가 크게 나타났다. 이는 토성이 세립질일 수록 유거수의 저항이 작기 때문으로 생각된다. 경사에 따라서는 경사도가 증가할수록 증가하였으며 $10\% 경사일 때를 기준으로 $Ro(mm)=Ro_{10}{\times}0.797{\times}e^{-0.021s(\%)}$로 나타났다.천성 승모판 폐쇄 부전등을 초래하는 심각한 선천성 심질환이다. 그러나 진단 즉시 직접 좌관상동맥-대동맥 이식술로 수술적 교정을 해줌으로써 좋은 성적을 기대할 수 있음을 보