• Microbiology and Biotechnology Letters
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• v.48 no.4
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• pp.506-514
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• 2020

4-Hydroxybenzyl alcohol (4-HB alcohol) is one of the major active components of Gastrodia elata Blume, with beneficial effects on neurological disorders such as headache, convulsive behavior, and dizziness. Here, we developed a metabolically engineered Corynebacterium glutamicum strain able to produce 4-HB alcohol from 4-hydroxybenzoate (4-HBA). First, the strain APS963 was obtained from the APS809 strain via the insertion of aroK from Methanocaldococcus jannaschii into the NCgl2922-deleted locus. As carboxylic acid reductase from Nocardia iowensis catalyzes the reduction of 4HBA to 4-hydroxybenzaldehyde (4-HB aldehyde), we then introduced a codon-optimized car gene into the genome of APS963, generating the GAS177 strain. Then, we deleted creG coding for a putative short-chain dehydrogenase and inserted ubiCpr encoding a product-resistant chorismate-pyruvate lyase into the pcaHG-deleted locus. The resulting engineered GAS355 strain accumulated 2.3 g/l 4-HB alcohol with 0.32 g/l 4-HBA and 0.3 g/l 4-HB aldehyde as byproducts from 8% glucose after 48 h of culture.

• Korean Journal of Plant Resources
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• v.35 no.1
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• pp.71-99
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• 2022

This study was conducted to investigate the vascular flora of Masan, located in Goseong-gun, Gangwon-do, and to comprehend the distribution of remarkable plants such as rare and Korea endemic plants and invasive alien plants. The survey was carried out the distribution of vascular plants 11 times per season from 2010 to 2018. A total of 619 taxa in 93 families, 328 genera, 529 species, 6 subspecies, 78 varieties and 6 forms were identified on mt. Masan. Among them, Korean endemic plants are 21 taxa including Saussurea diamantica Nakai and are plants designated by the Korea Forest Service are 20 taxa counting Saxifraga octopetala Nakai. Invasive alien plants were classified a total of 35 taxa inclusive of Trifolium pratense L. (WS), Aster pilosus Willd. (SS), Coreopsis lanceolata L. (SR), Rumex obtusifolius L. (SC) and Lindernia dubia (L.) Pennell (CS). The naturalization rate was 5% and the urbanization index was 10.0%, respectively. Although this study identified the diverse distribution of major plants such as rare and endemic plants around Masan, it was confirmed that invasive alien plants, which pose a great threat to forest biodiversity conservation and promotion, spread and distributed in a specific section Therefore, it is urgent to establish a conservation strategy and countermeasures.

• Conservation Science in Museum
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• v.26
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• pp.83-182
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• 2021

This study summarized the results of the conservation treatment and investigation on the production method of the golden crown (Treasure No. 339) excavated from Tomb No. 129 (also known as Seobongchong Tomb) in Noseo-dong, Gyeongju-si, Gyeongsangbuk-do Province. The golden crown from Seobongchong Tomb was discovered during the excavations conducted by the Museum of the Government-General of Korea in 1926 during the Japanese colonial era. It is currently in the collection of the National Museum of Korea. A total of six Silla golden crowns have survived in Korea, among which the crown from Seobongchong Tomb is the only example with a dome-shaped hemispherical decoration attached with a bird ornament while otherwise showing the typical features of Silla crowns. The crown had been repaired following its excavation using metallic materials and adhesives, but due to the partial deformation and damage brought about by the repair materials, it required further conservation treatment. This article describes in detail the overall process of the conservation treatment and the restoration of the original form of the golden crown from Seobongchong Tomb, particularly the method of reinforcing the joints to secure the stability of the crown. It presents the characteristics of the crown's production as revealed in the investigation during the conservation treatment, and further analyzes the relationship of this crown from Seobongchong Tomb with other Silla crowns through a comparison of their production techniques. The investigation revealed that the crown was primarily decorated with golden sequins at the time of its production. At a later point some of the sequins in the upright ornament were replaced with comma-shaped jade beads and additional comma-shaped jade beads were added to the headband. In order to determine if such modifications to the decoration had occurred with other Silla crowns, the decoration of the six extant Silla golden crowns were investigated. The crown from Cheonmachong Tomb features traces of this same modification to the decoration and possesses other similarities with the crown from Seobongchong Tomb.

• Journal of Korean Elementary Science Education
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• v.41 no.4
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• pp.616-626
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• 2022

This study aims to investigate the effect of the application of the brain-based learning model on the self-efficacy, creative problem-solving ability, and academic achievement of elementary school students in science classes. The participants consisted of 22 students from one class (experimental group) and 22 students from another class (comparison group) of J Elementary School in B Metropolitan city. The experimental group conducted science classes that applied the brain-based learning model, and the comparison group conducted general explanatory science classes according to textbooks and the guide books of the teachers. The study found that science classes that applied the brain-based learning model exerted positive effects on the three abovementioned skills. Based on the results, the study confirmed that the application of the model is an effective learning tool that increases the self-efficacy, creative problem-solving ability, and academic achievement of for elementary school students in science classes.

• Journal of Korean Medicine Rehabilitation
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• v.32 no.4
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• pp.1-8
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• 2022

Objectives This study is to investigate the effects and mechanisms of Gastrodia elata extract (GEE) on the high-fat diet-induced obesity model. Methods C57BL/6 mice were randomly assigned into 5 groups (n=10). Control group was fed normal diet (ND). Obesity group was fed 60% high fat diet (HFD). The other three groups were fed HFD with 100, 200, 500 mg/kg GEE. After five weeks, body weight, liver and epididymal fat weight, triglyceride concentration in liver and serum, sterol regulatory element-binding protein-1 (SREBP-1), acetyl-CoA carboxylase (ACC), fatty acid synthase, peroxisome proliferator-activated receptor 𝛾 (PPAR-𝛾), CCAAT/enhancer binding protein 𝛼 (C/EBP-𝛼) expression level, insulin concentration in serum were measured. Results The GEE (100, 200, and 500 mg/kg)-treated animals exhibited substantial decreases in body mass, liver weight and epididymal white adipose tissue collate to the HFD-fed group. GEE treatment also reduced hepatic and serum triglyceride level. Furthermore, GEE treatment significantly inhibited adipogenesis in the GEE group by reducing the protein expression of SREBP-1, ACC and the messenger RNA expression of PPAR𝛾, C/EBP-𝛼, which are adipocyte differentiation-related genes. Conclusions These research outcomes recommend that GEE is possibly valuable for the prevention of HFD-induced obesity via modification of various pathways related with adipogenesis and adipocyte differentiation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.2
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• pp.204-213
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• 1986

The objective of this paper was to examine the response of rice seedling emergence and early growth under the different temperature (day/night, 29/21$^{\circ}C$, 17/17$^{\circ}C$, 12/12$^{\circ}C$) and the different seeding depth (1 cm, 2 cm, 3 cm). The trial was carried out in the phytotron and field in the Crop Experiment Station, Suwon, Korea in 1985. Calcium peroxide-coated seeds were very effctive in promoting seedling emergence, seedling establishment and eary growth of rice. Coated seeds were more effective in low temperature condition (17/17$^{\circ}C$, 12/12$^{\circ}C$) than in high temperature (29/21$^{\circ}C$) at the phytotron trial. The deeper the seeding depth, the less the emergence and seedling establishment, and the available seeding depth was I cm in the direct seeding under the flooded soil. In the field trial (seeding date, May 1) the results for the emergence and seedling establishment were similar to those in the phytotron trial. Available cultivars for the direct seeding cultivation under the flooded soil were Cheonmabyeo, Namyangbyeo, Kihobyeo, Akibare, Nakdongbyeo and Dongjinbyeo in Japonica type, and Taebaegbyeo, Samgangbyeo and Gayabyeo in Indica x Jponica type. Coefficients of variation to the emergence and seedling establishment between rice cultivars were very high. Therefore, in the direct seeding cultivation under the submerged paddy, choice of rice cultivars and improved technique for direct seeding will be more necessary.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.4
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• pp.320-327
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• 1990

Experiments were conducted to investigate the method to raise seedling establishment rate and the optimum seeding dates and rates in direct seeding culture using transplanter under submerged field condition in Chuncheon area during 1986 to 1988. Direct seeding on submerged field using transplanting machine after sowing on seedling growing box contained with paddy or upland soil was possible. Higher rate of seedling establishment was obtained in case of sowing sprouted seed and intermittent irrigation after sowing. Seedling establishment rate was higher both in April 30 or May 10 seeding plot. Minimum daily average air and soil temperature to ensure more than 70 percent seedling establishment was 14.8$^{\circ}C$ and 16.3$^{\circ}C$ respectively. Optimum sowing date in the direct sowing was early May and optimum sowing rate was 200g per box, 6kg per l0a. In this case rice grain yield was 481kg per l0a and this is almost same yield level compared hand and machine transplanting.

• Journal of Life Science
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• v.33 no.3
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• pp.252-259
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• 2023

A decrease in ovarian function and sex hormone production with aging results in hormone deficiency, which causes menopause in women. Natural products have been investigated to develop estrogen-like substances to improve menopausal status in women. This study was performed to evaluate the efficacy of Gastrodia elata Blume extract (TVB-1000) in ovariectomized rats. After a recovery period of 7 days, the TVB-1000 treatment group was orally administered at 16, 40, and 100 mg/kg for 12 weeks. In the OVX-positive control group, 17β-estradiol was injected subcutaneously into the dorsal region of rats at a dose of 10 ㎍/kg. Results show that TG and LDL-C, which are cardiovascular disease markers, were significantly decreased in all concentrations of TVB-1000 administration in the treatment group, and the LDL-C/HDL-C ratio significantly decreased in the medium and high TVB-1000 concentrations of the treatment group. In addition, in the high concentration TVB-1000 treatment group, the weight of abdominal fat decreased with statistical significance. The low-dose and medium-dose administration groups showed statistical significance and the expression level of their ER-α increased, but ER-β did not show a significant change. The above experiment shows that owing to the estrogen-like effect of TVB-1000, it can be useful as a functional food material to prevent cardiovascular disease, which is one of the menopausal symptoms.

• Journal of Physiology & Pathology in Korean Medicine
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• v.36 no.6
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• pp.209-212
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• 2022

We recently reported that Gastrodia elata extracts (GEE) had an effects to protect against lipopolysaccharide-induced cognitive impairment in vivo model. In this study, we investigated the neuroprotective effects and the mechanism of action of GEE in hydrogen peroxide (H₂O₂)-induced cell death of SH-SY5Y human neuroblastoma cell. The SH-SY5Y cells were divided into five groups, including control(non-treated group), 100 μM H₂O₂, 100, 200, 500 ㎍/㎖ GEE+ 100 μM H₂O₂ groups. Pre- and co-treatment with GEE prevented cell death induced by 100 μM H₂O₂ for 24 h in SH-SY5Y cells. Our findings also showed that anti-oxidants enzymes (Cu/Zn superoxide dismutase, Mn superoxide dismutase, catalase) were up-regulated by 100 μM H₂O₂. But GEE suppressed H₂O₂-induced anti-oxidants enzymes decrease in a dose-dependent manner. Treatment with GEE also inhibited phosphorylation of eukaryotic initiation factor-2α (eIF-2α) and p38 by H₂O₂. Taken together, the neuroprotective effects of GEE in terms of recovery of antioxidant enzymes expression, down-regulation of eIF-2α and p38 phosphorylation, and inhibition of cell death are associated with reduced oxidative stress in SH-SY5Y cells.

• Korean Journal of Agricultural Science
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• v.16 no.1
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• pp.1-18
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• 1989

This experiment was undertaken to clarify derivation of resistance of Japonica type of rice varieties to rice blast in Korea and to classify Japonica type of rice varieties on the basis of their rice blast reaction in the blast nuisery test. 1. The resistance of Iljin, Kwancheok 9, Koshi, Baedal and Paldal, Jaekeon, Sinpung, Jinheung, Hokwang and Palkeum, and Gwangmyungbyeo and Yeongdeogbyeo to rice blast was derived from Kyudai Taicho Asahi 3, Futaba and 2067, respectively. 2. The resistance of Kwanok, Mankyeong and Nongbaek to the rice blast was derived from Kanto 55, Hokwang and Kusabue, and Ishigarisiroge, respectively. 3. The resistance of Seomjinbyeo, Sinseonchalbyeo, Donghaebyeo and Tamjinbyeo to the rice blast was derived from Milyang 20 and the source of resistance to the rice blast in Jinjubyeo and Daecheongbyeo was HR 769 or HR 1590. 4. The resistance of Dobongbyeo, Gwanagbyeo and Chiagbyeo to the rice blast was derived from Tjina, Kongo and Kuik 90, respectively. 5. The resistance of Seolagbyeo, Seonambyeo, Kihobyeo, Namyangbyeo, Samnambyeo, Seohaebyeo, Whaseongbyeo, Daegwanbyeo and Taeseongbyeo, and Sobaegbyeo, Odaebyeo and Unbongbyeo to the rice blast was derived from Fuji 280 and Fuji 269, respectively. 6. The source of resistance to the rice blast in Cheonmabyeo and Baegambyeo was BL 7 and Nongbaek, the resistance of Dongjinbyeo and Sangpungbyeo to the rice blast was derived from Satominori and Simokita, respectively. 7. Japonica type of rice varieties was classified into eleven varietal groups according to their reaction to the blast as follows. Eight varieties of Jinheung group, two varieties of Dongjinbyeo group, two varieties of Jinjubyeo group, three varieties of Gwanagbyeo group, four varieties of Sobaegbyeo group, one variety of Nongbaek group, two varieties of Baegambyeo group, five varieties of Sinseonchalbyeo group, five varieties of Seonambyeo group, two varieties of Taeseongbyeo group and some variety of Nagdongbyeo group.