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http://dx.doi.org/10.48022/mbl.2010.10008

Production of 4-Hydroxybenzyl Alcohol Using Metabolically Engineered Corynebacterium glutamicum  

Kim, Bu-Yeon (Major in Food Biotechnology, School of Food Biotechnology and Nutrition, Kyungsung University)
Jung, Hye-Bin (GeneChem Inc.)
Lee, Ji-Yeong (Major in Food Biotechnology, School of Food Biotechnology and Nutrition, Kyungsung University)
Ferrer, Lenny (Faculty of Biology, Bielefeld University)
Purwanto, Henry Syukur (Major in Food Biotechnology, School of Food Biotechnology and Nutrition, Kyungsung University)
Lee, Jin-Ho (Major in Food Biotechnology, School of Food Biotechnology and Nutrition, Kyungsung University)
Publication Information
Microbiology and Biotechnology Letters / v.48, no.4, 2020 , pp. 506-514 More about this Journal
Abstract
4-Hydroxybenzyl alcohol (4-HB alcohol) is one of the major active components of Gastrodia elata Blume, with beneficial effects on neurological disorders such as headache, convulsive behavior, and dizziness. Here, we developed a metabolically engineered Corynebacterium glutamicum strain able to produce 4-HB alcohol from 4-hydroxybenzoate (4-HBA). First, the strain APS963 was obtained from the APS809 strain via the insertion of aroK from Methanocaldococcus jannaschii into the NCgl2922-deleted locus. As carboxylic acid reductase from Nocardia iowensis catalyzes the reduction of 4HBA to 4-hydroxybenzaldehyde (4-HB aldehyde), we then introduced a codon-optimized car gene into the genome of APS963, generating the GAS177 strain. Then, we deleted creG coding for a putative short-chain dehydrogenase and inserted ubiCpr encoding a product-resistant chorismate-pyruvate lyase into the pcaHG-deleted locus. The resulting engineered GAS355 strain accumulated 2.3 g/l 4-HB alcohol with 0.32 g/l 4-HBA and 0.3 g/l 4-HB aldehyde as byproducts from 8% glucose after 48 h of culture.
Keywords
4-hydroxybenzyl alcohol; Gastrodia elata; Corynebacterium glutamicum; metabolic engineering; carboxylic acid reductase;
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Times Cited By KSCI : 5  (Citation Analysis)
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