• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제26권5호
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• pp.437-445
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• 2000

Object This study is focused on the comparison between $CO_2$ laser and scalpel by examining the overal process of wound healing after partial glossectomy of rats so that the result from the research could be easily applied to actual clinical environment. Method and Material In this research, 2mm defect was formed on both lateral borders of the tongue of rats by using $CO_2$ laser and scalpel respectively. 4rats were sacrified each time on the 1st, 2nd, 4th, 7th, 14th and 21th day according to a general method. After fabricating $5{\mu}m$ paraffin specimen, H&E staining and MT staining were performed. Results Compared to scalpel, wound healing by $CO_2$ laser leads to a earlier initiation of vascular proliferation and re-epithelization. Therefore, some influence is exerted on the early stage of wound healing. However, the two methods seem to undergo the similar healing process.

• 한국임상수의학회지
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• 제11권1호
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• pp.427-436
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• 1994

To investigate the effect of yeast glucan on wound healing in rabbits, histopathological findings and wound contraction after topical application of glucan on the open wound were observed. Formalin fixation and embedding of tissues at wound were prepared for histopathological findings at the other day after wounding. Wound size was measured for contraction. Macrophages appeared in the deeper site under the scab at day 1 after wounding in the glucan treated group, but not in the control. A number of macrophages, fibroblasts and a small number of neutrophils were observed at day 3 after wounding In the glucan treated group as compared with the control. Histopathological findings at day 5 after wounding in control were similar with those at day 3 in the glucan treated group. Glucan like granules were scattered in the cytoplasm of macrophages at day 5 in the glucan treated group. A great portion of wound reepithelialized at day 5 in the glucan treated group but a small portion of wound in the control. The Present study indicated that the treatment of 5,000$\mu\textrm{g}$/$m\ell$ of glucan has an effect on wound heal. ins in respect to migration of macrophage into the wound site, granulation tissue formation and epithelialization.

• 한국전문물리치료학회지
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• 제6권3호
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• pp.1-10
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• 1999

The purpose of this study was to determine whether high voltage pulsed current stimulation (HVPCS) would enhance wound healing in neuropathic rabbits. Ten rabbits were assigned to either an experimental or a control group. The wounded part around the peripheral neuropathy of the experimental rabbits was stimulated for two hours twice a day for six days under the following conditions: pulse frequency 80 pps, pulse duration $100{\mu}s$, and stimulation intensity 30~40 V. The results indicated that there was no difference in the wound closure between the experimental and control groups. The two groups showed similar aspects in collagen and reticulum, which were observed by colored Masson's trichome. While the rabbits in the control group had more or less thick fibers, the rabbits in the experimental group had thin and branched-shape fibers. The rabbits in the experimental group showed both strong responses in the shaping of elastic fibers and the increased aspects in fibroblast when compared with the control group.

• 대한수의학회지
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• 제37권3호
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• pp.629-637
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• 1997

This study was performed to determine the optimal dose of laser energy for wound healing. The skin wound with 8mm diameter was induced over the lumbar vertebrae of the rats, and wound squares, scab hardness, hematologic findings and histopathlogic findings according to irradiation of laser energy were studied. 1. Wound square was significantly reduced at Day 1 (p<0.01), 2 (p<0.01), 3 (p<0.05), 7 (p<0.01) and 8 (p<0.05), respectively in experimental groups, especially group II, compared with control group. 2. Scab hardness was significantly increased at Day 1 (p<0.01), 2 (p<0.01), 3 (p<0.05), 5 (p<0.01) and 7 (p<0.01), respectively in experimental groups, especially group II, compared with control group. 3. In hematological findings, red blood cells and white blood cells in experimental group were increased according to the lapse of days, but they were not significant. 4. In histopathologic findings, experimental groups, especially group II, revealed early scab formation, early appearance of phagocytes and fibroblast, rapid growth of granulation tissue and collagen, and promotion of wound healing in the result.

• Journal of Oral Medicine and Pain
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• 제7권1호
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• pp.5-26
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• 1982

In order to study the effects of traimcinolone acetonide on the wound healing of tongue, eight two healthy albino rats were asesthetized with pentothal. The wound, approximately 1mm in depth and 2mm in diameter, was created on the midlateral aspect of tongue with the tip of small rongeur fourcep. Forty one animals were employed in one of the following groups:(I) Control group: Received 0.5ml/100gm B.W. of saline suspension intralesionally at weekly interval until sacrificed.(II) Experimental group: Received $100\mu\textrm{g}/100gm$ B.W. of trimacinolone acet- onide suspension intralesionally at weekly interval until sacrificed. Biopsy specimens of wounds were taken at 6,18,24 hours, 2,3,5,7,14 and 28 days after wounding. The specimens were sectioned, and observed with light microscope and electron microscope. The results were as follows : 1. In the triamcinolone acetonide treated wound, the epithelial and fibroblastic regeneration were dealayed when compared with controls. 2. After triamcinolone acetonide treated, edema was decreased at the early stage of wound healing. 3. In the triamcinolone acetonide treated wound, the fibroblasts exhibited fine morphological changes and reduction of collagen formation. It seems that triamcinolone acetonide inhibits fibroblast function.

• 대한기관식도과학회:학술대회논문집
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• 대한기관식도과학회 1991년도 제25차 학술대회 연제순서 및 초록
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• pp.28-28
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• 1991

후두암의 수직절제 수술후의 후두 재건 목적은 기관절개를 통하지 않고 후두를 통한 호흡이 가능하도록 하면서 연하시 기도흡인을 피하기 위한 sphincter 능력의 보존, 그리고 발성이 가능하도록 성대진동 mechanism을 재건하는데 있다. 오늘날 수많은 후두학자들에 의해서 고안 개발된 다양한 재건 방법이 있다. 연자는 광범위한 후두절제에 따른 큰 결손을 메우기 위해 bulky하면서도 점막 상피의 재생이 용이한 재건 피판으로 흉유돌근골막 피판을 이용하여 후두 결손부를 재건하여 보았다. 방법은 성견 3마리를 대상으로하여 thiopental sodium 정맥주사로 전신마취를 시행한 후 설골에서부터 흉골상까지 경부 정중앙의 피부를 절개하고 후두를 노출시켜 후두 수직절제술을 시행하였다. 흉유돌근과 흉골에 부착된 골막을 박리하여 흥유돌근골막 피판을 제작한 후 골막이 후두강 안쪽으로 되도록하여 골막연과 후두점막을 봉합하였다. 그리고 3, 5, 9개월에 후두적출술을 시행하여 병리조직학적 및 수술후 경과를 관찰하였다. 3실험견 모두 기관절개술 없이 후두를 통한 호흡이 가능하였고 흡입성 폐렴이나 피하기종등의 합병증없이 창상이 치유되었으며 발성도 양호하였다. 이식된 골막위로 신생혈관의 출현과 함께 점막재생이 잘되 있었다. 단지 봉합사 주위에 소량의 육아종이 관찰되었다. 조직학적으로는 섬유조직위로 편평상피가 재생되었으며 성문하부에서는 일부 섬모가 있는 호흡기 점막도 관찰되고 골막하부에 신생골 형성은 관찰할 수 없었다. 골막 피판은 그 유연성 때문에 결손부위의 점막연에 맞춰도 tension이 없고 공기 누출이 되지 않게 봉합이 가능할 뿐 아니라 점막이 재생할 수 있는 frame-work의 역할을 하는 것으로 사료되었다. 이상과 같은 사실로 미루어 흉유돌근 자체가 견실하고 골막에 혈류공급이 잘되어 창상치유에 좋을 뿐 아니라 큰 후두결손부의 재건이 가능하리라고 사료되었다.로서 몇가지 앞으로의 치료지침에 도움이 되는 결과를 얻었기에 보고하는 바이다. 1) 성별 분포는 남자 16(39 %), 여자 25 (61%)이었으며 1 : 1.5의 빈도를 보였다. 2) 연령 분포는 20대와 30대에서 남녀 모두 25명으로 대부분을 차지하였다. 3) 부식제의 종류는 빙초산이 26명 (63.4 %)으로 대부분을 차지하였고 염산 7 (17.1 %) Lye 3 (0.7 %) 의 순이었다. 4) 음독후 12시간내에 식도경술을 받은 환자가 3명(0.7 %) 12-24시간에 받은 환자가 17명(41.5 %), 24 - 48시간에 받은 환자가 11명(26. 8%)으로 48시간 내에 시행받은 환자가 전체의 75.6%를 차지하였다. 5) 식도경 검사상 나타난 식도화상은 Grade I 11명 (26.8%) G.ade II 18(43.9%) Grade III 7명(17.1%) 이었으며 Grade II 인 경우가 18명(43.9%)로 가장 많았으며 Grade I 11명(26.8 %), Grade III 7명 (17.1 %) Normal 5명 (12.2 %) 순이었다. 6) 조기 식도경 검사에서 41명중 oral cavity burn이 없었던 경우가 15명(36. 1 %) 이었으며, oral cavity burn이 있었던 26명중 5명(19 .2 %)에서 Esophageal burn이 없었다 특히 Esophageal burn의 Grade II, III 25명 중 9명(29.6 %)에서 oral cavity burn이 없었다. 7) 식도 부식중 환자의 치료 원칙으로 Grade I, II, III에서 항생제 및 보존적 치료를 하였으며 Grade I에선 oral feeding을 시켰고 Steroid는 경우에 따라 투여하였으며 Grade III에선 원칙적으로 사용치 않았다. 식도조영술은 Grade I II III에서 3주 후에 모두 시행하였다. 8) 3주 후 식도조영술을 실

• Archives of Plastic Surgery
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• 제34권5호
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• pp.551-556
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• 2007

Purpose: Pseudomonas aeruginosa is an etiologic agent in serious wound infection. Pseudomonas aeruginosa infection is problematic because this organism is resistant to many antimicrobial drugs. The purpose of this study was to compare the bactericidal effect of commonly used topical agents and their effect on wound healing. Methods: Pseudomonas aeruginosa-infected full-thickness skin defect was developed on the mouse to compare 3 commonly used topical agents-Betadine, 2% Gentamicin solution and 0.3% Acetic acid with the control group. Wound size change, bacterial colony counts and histologic findings of each groups were analyzed. Results: The wound size decreased in all treated groups as compared with the control group. However, there was no statistical difference. Gentamicin solution group was showed the lowest bacterial colony count and statistically significant difference compared with the control group(p=0.032). Other treated groups were also effective against Pseudomonas aeruginosa, but not different statistically. Histologic findings revealed that epithelialization, granulation tissue formation and microvessel proliferation were increased and necrosis and inflammation were decreased in all treated groups compared to the control group, but not different statistically. Betadine group significantly increased granulation tissue formation compared to the control group (p= 0.041). Conclusion: There is no universal topical agent that enhances most aspects of wound healing while simultaneously decreasing the bacterial concentration. However, Gentamicin solution may be an optimal topical agent for Pseudomonas aeruginosa infected wound. Further study should experiment on human with Gentamicin solution to confirm a effect on Pseudomonas aeruginosa infected wound for clinical applications.

• 폴리머
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• 제37권4호
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• pp.462-469
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• 2013

Catechin(CTEC)은 천연항산화제로 알려져 있고 항염증에 효과적이며 피부 창상치료제로 응용되고 있다. 이 연구는 ${\beta}$-cyclodextrin(${\beta}$-CD)으로 나노입자화한 CTEC을 poly(vinyl alcohol)(PVA)/pectin(PT) 조성의 하이드로젤에 첨가하였다. 피부상처의 재상피화를 위해 CTEC과 ${\beta}$-CD을 분자복합(molecular complex) 방법에 의해 나노입자 제조를 수행하였다. 하이드로젤에 첨가된 CTEC 나노입자는 $250{\pm}17.5$ nm이며, 입자 내 CTEC의 담지 효율은 처음첨가량의 74%가 담지되었음을 확인하였다. CTEC는 하이드로젤 필름으로부터 pH7.4와 pH5.5 버퍼에서 72시간 동안 각각 $86.51{\pm}3.14%$와 $35.95{\pm}2.14%$의 방출을 보였다. 창상치료 실험에서 CTEC 나노입자가 적재된 PVA/PT 조성의 하이드로젤은 CTEC이 함유된 도포형 젤보다 빠른 치유력을 관찰할 수 있었다.

• Archives of Plastic Surgery
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• 제36권5호
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• pp.519-524
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• 2009

Purpose: This study was conducted to establish the most effective method of cell therapy by comparing and analyzing the level of wound healing after various cell delivery methods. Methods: Human mesenchymal stem cells were administered using 5 different methods on full thickness skin defects which were deliberately created on the back of 4 - week old mice using a 8 mm punch. Different modes of administration, cell suspension, local injection, collagen GAG matrix seeding, fibrin, and hydrogel mix methods were used. In each experiment group, $4{\times}105$ mesenchymal stem cells were administered according to 5 deferent methods, and were not for the corresponding control group. Results: The wound healing rate was fastest in the local injection group. The wound healing rate was relatively slow in the collagen matrix group, however, the number of blood vessels or VEGF increased most in this group. Conclusion: For rapid wound healing through wound contraction, it is advantageous to administer MSC by the local injection method. For the healing process of a wide area, such as a burn, the seeding of cells to collagen matrix is thought to be effective.

• 미생물학회지
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• 제55권2호
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• pp.87-102
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• 2019

대부분의 만성창상(chronic wounds)은 생물막으로 인해 상처 치유시 염증단계를 지속시킨다. 생물막은 항생제(antibiotics)에 대한 저항성을 가지며 침투력을 저하시키고 살균제(biocides)에 대한 내성을 지니며 국소면역반응을 약화시킨다. 또한 생물막은 주변의 조직에 단단히 붙어 있어 제거하는 작업이 매우 어렵다. 그러므로 주변 조직을 손상시키지 않으면서 단단한 생물막을 제거하는 전략은 매우 중요하다. 그 중에 하나가 분산기작을 이용한 생물막의 해체이며 지금까지 많은 연구가 수행되어 왔다. 본 고찰논문에서는 특별히 화학주성, 파지요법, 다당류, 다양한 효소(당분해효소, 단백질분해효소, DNA 분해효소), 계면활성제, 분산신호, 자기유도인자, 조절인자, 억제제 등이 소개되었으며 더 나아가 항생제 치료 및 다른 치료와의 병행을 통한 병합요법도 소개되었다. 앞으로 본 논문에서 제시된 생물막의 분산기작의 지식을 이용하여 만성 창상 감염치료의 가능성이 더 높아지길 기대한다.