Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
권호 표지

Comparison of Topical Agents for Bactericidal and Wound Healing Effect in Pseudomonas aeruginosa-infected Wound

Pseudomonas aeruginosa 감염창상에 사용되는 국소제제들의 항균효과 및 창상치유 효과의 비교

  • Min, Kyung Hee (Department of Plastic and Reconstructive Surgery, Eulji Medical College) ;
  • Hong, Sung Hee (Department of Plastic and Reconstructive Surgery, Eulji Medical College) ;
  • Kim, Eun Kyung (Department of Pathology, Eulji Medical College)
  • 민경희 (을지대학교 의과대학 성형외과학교실) ;
  • 홍성희 (을지대학교 의과대학 성형외과학교실) ;
  • 김은경 (을지대학교 의과대학 병리학교실)
  • Received : 2007.04.04
  • Published : 2007.09.10
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: Pseudomonas aeruginosa is an etiologic agent in serious wound infection. Pseudomonas aeruginosa infection is problematic because this organism is resistant to many antimicrobial drugs. The purpose of this study was to compare the bactericidal effect of commonly used topical agents and their effect on wound healing. Methods: Pseudomonas aeruginosa-infected full-thickness skin defect was developed on the mouse to compare 3 commonly used topical agents-Betadine, 2% Gentamicin solution and 0.3% Acetic acid with the control group. Wound size change, bacterial colony counts and histologic findings of each groups were analyzed. Results: The wound size decreased in all treated groups as compared with the control group. However, there was no statistical difference. Gentamicin solution group was showed the lowest bacterial colony count and statistically significant difference compared with the control group(p=0.032). Other treated groups were also effective against Pseudomonas aeruginosa, but not different statistically. Histologic findings revealed that epithelialization, granulation tissue formation and microvessel proliferation were increased and necrosis and inflammation were decreased in all treated groups compared to the control group, but not different statistically. Betadine group significantly increased granulation tissue formation compared to the control group (p= 0.041). Conclusion: There is no universal topical agent that enhances most aspects of wound healing while simultaneously decreasing the bacterial concentration. However, Gentamicin solution may be an optimal topical agent for Pseudomonas aeruginosa infected wound. Further study should experiment on human with Gentamicin solution to confirm a effect on Pseudomonas aeruginosa infected wound for clinical applications.

Keywords

References

  1. Mathes SJ: Plastic Surgery. 2nd ed., Philadelphia, Saunders Elsevier Inc., 2006, p 220
  2. Kim JI, Shin DH, Lee SK, Kim HJ, Choi JK: The effect of Yunkyo on open wound healing in rats. J Korean Soc Plast Reconstr Surg 31: 206, 2004
  3. Gamelli RL: International society for bum injuries survey: infection and infectious complications in worldwide burn units. Burns 30: 655, 2004 https://doi.org/10.1016/j.burns.2004.04.004
  4. Kwon H, Chang IS, Lee CK, Lim P: Clinical observation of the pressure sore. J Korean Soc Plast Reconstr Surg 23: 818, 1996
  5. Rosenthal M, Pfaller K: Medical Microbiology. 4th ed., St. Louis, Mosby Inc., 2002, P 297
  6. Mathes SJ: Plastic Surgery. 2nd ed., Philadelphia, Saunders Elsevier Inc., 2006, p 883
  7. Ulkur E, Oncul O, Karagoz H, Celikoz B, Cavuslu S: Comparison of silver-coated dressing($Acticoat^{TM}$), chlorhexidine acetate 0.5% ($Bactigrass^{\circledR}$), and silver sulfadiazine 1% ($Silverdin^{\circledR}$) for topical antibacterial effect in Pseudomonas aeruginosa-contaminated, full-skin thickness burn wounds in rats. J Burn Care Rehabil 26: 430, 2005 https://doi.org/10.1097/01.bcr.0000176879.27535.09
  8. Gilman AG, Goodman LS, Rall TW, Murad F: GOODMAN and GILMAN's the pharmacological basis of therapeutics. 7th ed., New York, Macmillan publishing company, 1985, P 1150
  9. Pyo HC, Kim YK, Whang KU, Park YL, Eun HC: A comparative study of cytotoxicity of topical antimicrobials to cultured human keratinocytes and fibroblasts. Korean J Dermatol 33: 895, 1995
  10. Damour O, Hua SZ, Lasne F, Villain M, Rouselle P, Collombel C: Cytotoxicity evaluation of antiseptics and antibiotics on cultured human fibroblasts and keratinocytes. Burns 18: 479, 1992 https://doi.org/10.1016/0305-4179(92)90180-3
  11. Reimer K, Wichelhaus TA, Schafer V, Rudolph P, Kramer A, Wutzler P, Ganzer D, Fleischer W: Antimicrobial effectiveness of povidone-iodine and consequences for new application areas. Dermatology 204: 114, 2002 https://doi.org/10.1159/000057738
  12. Bennett LL, Rosenblum RS, Perlov C, Davidson JM, Barton RM, Nanney LB: An in vivo comparison of topical agents on wound repair. Plast Reconstr Surg 108: 675, 2001 https://doi.org/10.1097/00006534-200109010-00011
  13. Vehmeyer-Heeman M, Van den Kerckhove E, Gorissen K, Boeckx W: Povidone-iodine ointment: no effect of split skin graft healing time. Burns 31: 489, 2005 https://doi.org/10.1016/j.burns.2004.11.018
  14. Brennan SS, Leaper DJ: The effect of antiseptics on the healing wound: a study using the rabbit ear chamber. Br J Surg 72: 780, 1985 https://doi.org/10.1002/bjs.1800721004
  15. Lineaweaver W, McMorris S, Soucy D, Howard R: Cellular and bacterial toxicities of topical antimicrobials. Plast Reconstr Surg 75: 394, 1985 https://doi.org/10.1097/00006534-198503000-00016