• Journal of Veterinary Clinics
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• v.21 no.2
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• pp.93-96
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• 2004

We examined the responses of PBMCs to house dust mite (HDM) allergen in atopic and healthy, non-atopic dogs to identify differences in lymphocyte reactivity that might reflect the immunologic status of atopic dermatitis. Thirteen of 20 (65%) atopic dogs showed a positive lymphocyte proliferative response to HDM allergen. The rate of response was significantly higher in the atopic dogs than that in healthy, non-atopic dogs insensitive to the allergen (P = 0.007). The proliferative responses were positively correlated with the level of HDM-specific IgE in serum (P = 0.035), and were thereby confirmed to reflect the activity of lymphocytes competent to promote IgE production. These results suggest that HDM-specific lymphocytes were present in peripheral blood and played a role in the pathogenesis of canine atopic dermatitis.

• Parasites, Hosts and Diseases
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• v.35 no.3
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• pp.149-154
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• 1997

A storage mite, Tyrophcgus putrescentiae, is recently known to be widely distributed in Korea, being commonly found in house dust, and may, therefore, be allergenically important. The purpose of this study was to develop mass rearing techniques for supplying a large quantity of allergens. The laboratory mouse food powder gave the highest yield, showing 1,251.5-fold increase in number after 10 weeks, and the mixed powder of laboratory mouse food and yeast (1 : 1) also gave same level of the production (1.203 1-fold increase in week 10). Several different combinations of temperature and relative humidity conditions were compared, and the maximum propagation was obtained at $25^{\circ}C$ and 64% RH, showing 960-fold increase in number. When the same amount of culture media was used the size of the culture container did not significantly influence the quantitative yield of T. putrescentice mites.

• Korean Journal of Human Ecology
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• v.22 no.6
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• pp.687-699
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• 2013

This study was carried out to investigate the antifungal activity and house dust mite repellent effect of cotton and silk fabrics dyed with Juniperus chinensis heartwood extracts by hot-water and methanol. The chemical composition of Juniperus chinensis heartwood extracts by hot-water and methanol was analyzed by GC-MS. In the case of antifungal activity, dyed fabrics with hot water extract had no effect against chaetomium globosum fungus. But showing no growth in fabrics dyed with methanol extract, dyed fabrics with methanol extract showed very excellent antifungal activity. Silk fabric dyed with methanol extract showed excellent house dust mite repellent effect of 94.3%~96.0% against dermatophagodes farinae. The composition of Juniperus chinensis heartwood extracts from hot-water and methanol was different. There were no terpene and its analogue peaks in hot-water extract. However there were terpene and its analogue peaks in methanol extract. From these GC-MS results, terpene was identified in methanol extract of Juniperus chinensis heartwood. Efficient ingredient of antifungal activity and house dust mite repellent effect was assumed terpene.

• Clinical and Experimental Pediatrics
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• v.49 no.6
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• pp.643-647
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• 2006

Purpose : Recently, incidences of chronic cough with allergic disease have been increased. The identification of specific allergens for chronically coughing children is significant for diagnosis, proper treatment and prevention. The aim of this study was to identify significant contributors for sensitization in chronically coughing children. Methods : A total of 106 children, aged 1-6 years, visiting for chronic cough(>3 weeks), were tested for inhalant multiple allergosorbent assay(MAST), eosinophil count and total IgE. Parents completed a questionnaire on past history of atopy(infantile eczema) and bronchiolitis, family history of allergy, breastfeeding, smoking, pets and floor coverings at home. Results : Significant contributors for sensitization were : age(4-6 years), infantile eczema, positive family history of allergy and smoking by family. Conclusion : We were able to identify significant contributors for sensitization in chronically coughing children.

• Research in Community and Public Health Nursing
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• v.13 no.1
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• pp.79-88
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• 2002

Purpose: The purpose of this study was to identify the indoor environment i.e. house type and age, ventilation method, use of bed & sofa, cockroach, ants, etc. on HDM (House dust mites), especially Der fI allergen. Method: Samples of dust from mattresses, pillows and the floor were collected by using a vacuum cleaner from April. 2000. The amount of Group I allergen(Der. fI) of HDM (house dust mites) were measured by two-site ELISA. Indoor Environmental characteristics were accessed by using questionnaires on 178 house wives living in the Seoul area. Results: The amount of HDM allergen (Der fI) was higher in sofa using house. There were higher concentrations of HDM allergen (Der fI) in sofas made from quilt material than for those that were made from leather. Homes that had pets like dogs living in them had higher concentrations of HDM allergen (Der fI) than without dogs. Conclusion: The photophobic mites thrive in dark. warm and humid environments; Items such as pillows. mattresses, box springs, blankets. carpets. and upholstered furniture should be considered ideal environments for the mite. Therefore, an indoor environmental control program should be carried out to avoid the HDM allergen.

• Journal of Veterinary Clinics
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• v.19 no.2
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• pp.215-218
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• 2002

It is known that house dust mites which settle on sofa, carpet, and dust in the house ignite asthma and allergic rhinitis. Dermatophagoides farinae and Dermatophagoides pteronyssinus distribute widely and densely in Korea. In this study we carried out to identify that shed hair of dogs which was applied topically with fipronil ($\Frontline^{circledR}$) kill house dust mites. We co-cultured house dust mites with fipronil-applied hair of dog during 12 hours, and then compared the death rate of mites here with that cultured without hair. The larger amount of hair, the more mites were killed. The death rate of mites was higher than control significantly until 2 weeks after $\Frontline^{circledR}$ application. Hair which had been shed at 3 days after $\Frontline^{circledR}$ application always killed the smaller number of mites than not shed. The present study suggested that the use of $\Frontline^{circledR}$ to our pets control house dust mites effectively.

• Proceedings of the Korean Environmental Health Society Conference
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• 2005.11a
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• pp.104-105
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• 2005

알레르기 유발물질은 알레르기 질환 뿐 아니라 호흡기 질환과 폐기능에도 영향이 있다고 알려져 있다. 이에 알레르기 항원에 따른 폐기능의 영향을 본 결과 실내 알레르겐 항원인 집먼지 진드기와 곰팡이가 폐기능에 영향을 준다고 나타났고 이러한 항원들이 살 수 있는 환경에서도 폐기능이 영향을 받았다. 이는 실내의 환기나 청결상태가 개인의 건강에 영향을 미칠 수 있다는 것을 본 연구가 뒷받침해 준다.

• The Journal of Pediatrics of Korean Medicine
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• v.35 no.4
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• pp.167-167
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• 2021

• Korean Chemical Engineering Research
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• v.60 no.4
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• pp.557-563
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• 2022

In order to measure the allergen penetration of micropore fabrics, it is necessary to develop a convenient and appropriate experimental method for measuring a pore size of micropore fabrics. In this study, a simple and economical experimental apparatus was developed for the analysis of the pore size of micropore fabrics by measuring the weight reduction rate. In addition, the allergen blocking properties was evaluated by measuring the pore sizes of various fabrics. According to this study, the size of the pores of the microporous fabric could be obtained by measuring the weight reduction rates. In addition, higher weight reduction rate was obtained as the suction pressure passing through the particle permeation device decreased and the suction time was increased. It is expected that the developed experimental method and apparatus can be utilized as an experimental standard for quality control methods to verify the effectiveness of micropore fabrics used for house dust mite blocking bedding.

• Journal of Korean Public Health Nursing
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• v.16 no.1
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• pp.30-44
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• 2002

The purpose of this study were to identify seasons variation of house dust mites allergen and perceived allergic symptom. The subjects were consisted of 29 family with allergy patients and 34 family without allergy patients. Perceived allergic symptoms were accessed and house dust was sampled from beds, floor of bedroom, kitchen and livingroom in spring(August), summer (July), fall(October) and winter(January) and tested using two-site ELISA. The results were as follows; 1) In all family, amount of house dust mites allergen(Der fI) of livingroom floor was the highest in summer$(4.73{\mu}g/1g\;of\;dust)$, and then fall$4.67{\mu}g/1g\;of\;dust)$, winter$(3.94{\mu}g/1g\;of\;dust)$, spring$(1.73{\mu}g/1g\;of\;dust)$. 2) In family with allergy patients, amount of house dust mites allergen(Der fI) of bedroom floor was highest in fall $(9.75{\mu}g/1g\;of\;dust)$. 3) In family with allergy patients, amount of house dust mites allergen(Der fI) of mattress was highest in fall$(8.23{\mu}g/1g\;of\;dust)$. 4) Perceived allergic symptom scores of family with allergy patients were higher than family without allergy patients in all seasons. In family without allergy patients, perceived allergic symptom scores was the highest in spring(4.29) and perceived allergic symptom scores of family with patients was the highest in winter(2.49). 5) The relationship of house dust mites allergen and perceived allergic symptom scores were positively related (r=.941, p=.000). Perceived allergic symptoms were correlated with amount of house dust mites allergen, That is, perceived allergic symptoms were became worse by house dust mites allergen. So House dust mites allergen reducing strategies and intervention should be recommended in further study.