• Journal of Life Science
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• v.20 no.1
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• pp.97-102
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• 2010

Streptomyces platensis YK-2, newly isolated from forest soil, produces transglutaminase (TGase), which catalyses an acyl transfer reaction between the primary grade amine and protein or $\gamma$-carboxyamide group of peptide bound glutamine residues. For a molecular genetic study of S. platensis, an effective transformation method was established by using a conjugal transfer of DNA from Escherichia coli to spores of actinomycetes. The highest transconjugation frequency of S. platensis was obtained on an MS medium containing 50 mM $MgCl_2$, using $5{\times}10^7\;E$. coli as a DNA donor and $1{\times}10^8$ spores without heat treatment as a host. We also identified that S. platensis contains a single attB site within an ORF encoding a pirin-homolog, and that its attB site sequence shows high homology to that of S. logisporoflavus. In addition, it was confirmed by phenotypic analyses of exconjugants that the introduction of heterologous DNA into the attB site of the S. platensis chromosome does not affect its morphological differentiation and TGase production.

• Korean Journal of Microbiology
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• v.31 no.1
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• pp.93-98
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• 1993

To investigate the organic matter transformation in aquatic environment, seasonal fluctuations of heterotrophic activity and microbia] extracellular enzyme activity were studied in Paldang Lake, Korea. The turnover time in the water column and the sediment at the station I fluctuated between 3 -I ,300 hrs and 17-170 hrs for glucose, 5 -1.900 hrs and 15-240 hrs for protein hydrolysate and 4-350 hrs and 15-230 hrs for acetic acid, respectively, indicating that the seasonal turnover time of organic substrates fluctuated drastically. The respiration ratios of glucose. protein hydrolysate and acetate were 23-32%, 38-41% and 22-28% in the water column and 34%, 61% and 41% in the sediment. respectively. These results showed that the respiration ratios in the sediment were higher than those in the water column regardless of kinds of organic substrates. The bacterial extracellular enzyme activities of $\alpha$-glucosidase. $\beta$-glucosidase, N-acetyl-$\beta$-D-glucosaminidase and aminopeptidase were 32-44%. 31-32%, 18-34% and 61-67% in the water column, and 34%. 40%, 23% and 65% in the sediment. respectively.

• Proceedings of the Korean Association of Geographic Inforamtion Studies Conference
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• 2004.10a
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• pp.27-40
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• 2004

When we derive multi-scale databases from a source spatial database, thegeometries and topological relations in the source database are transformed according to a predefined set of constraints. This means that the derived databases should be checked to see if the constraints are respected during the construction or updates of databases and to maintain the consistency of multi-scale databases. In this paper, we focus on the topological consistency between the source and derived databases, which is one of the important constraints to respect. In particular, we deal with the method of assessment of topological consistency, when 2-dimensional objects are collapsed to 1-dimensional ones. We introduce eight types of topological relations between 2-dimensional objects and 19 topological ones between 1-dimensional objects and propose four different strategies to convert 2-dimensional topological relations in the source database to 1-dimensional ones objects in the target database. With these strategies, we guarantee the topological consistency between multi-scale databases.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.9B
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• pp.1713-1720
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• 1999

This paper presents a new key frame extraction technique, for scene change detection, using the proposed AHIM (Accumulative Histogram Intersection Measure) from the DC image constructed by DCT DC coefficients in the compressed video sequence that is video compression standard such as MPEG. For fast content-based browsing and video retrieval in a video database, we also provide a novel coarse-to-fine video indexing scheme. In the extracted key frame, we perform the region segmentation as a preprocessing. First, the segmented image is projected with the horizontal direction, then we transform the result into a histogram, which is saved as a database index. In the second step, we calculate the moments and change them into a distance value. From the simulation results, the proposed method clearly shows the validity and superiority in respect of computation time and memory space, and that in conjunction with other techniques for indexing, such as color, can provide a powerful framework for image indexing and retrieval.

• Journal of the Korea Convergence Society
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• v.9 no.11
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• pp.491-500
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• 2018

The purpose of this study is to identify the types of nursing organization culture recognized by hospital nurses and to confirm the relationship between these nursing organizational culture and professional quality of life. A convenience sampling method was used to collect data from 216 participants who worked in a university hospital in C city, Chungbuk province. Data were analyzed using SPSS / WIN22.0 program. The significant predictors of Compassion Satisfaction, which is a subdivision of the professional quality of life, include Innovation-oriented culture, marital status, and the relationship - oriented culture. As for secondary traumatic stress, it has the significant predictors such as age, task-oriented culture and hierarchy-oriented culture. Burnout also turns out to be affected by the predictors of relationship - oriented culture, clinical career, and hierarchy-oriented culture. Based on these results, to create a innovation-oriented culture and relation-oriented culture within the organization, it is necessary to develop an intervention program for nurses' recognition change and improvement, in addition, studies that test the clinical effects are needed. In order to secure skilled nurses with rich clinical and career abilities, the working environment and treatment should be improved at the organization level.

• 재활복지
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• v.22 no.2
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• pp.83-102
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• 2018

The purpose of the this study was to investigate the association of self-stigma, quality of life, and self-esteem and analyze the mediating role of self-esteem in the relationship between self-stigma and quality of life among people with mental disabilities. For this purpose, the data were collected from 254 people with mental disabilities who utilize the community mental health centers located in D and K regions. Findings of the study indicated that there were statistically significant relationships among self-stigma, quality of life and self-esteem. In addition, self-esteem had partial mediating effects on the relationships between self-stigma and the quality of life domains such as physical, psychological, and environmental areas, while self-esteem was a full mediator in the relationships between self-stigma and social relationship in the quality of life. Based on the findings of, possible practice implications for improving the quality of life among people with mental disabilities by reducing self-stigma along with empowering their self-esteem and recommendation for further study were provided.

• Journal of Practical Engineering Education
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• v.13 no.2
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• pp.283-292
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• 2021

As universities have operated non-face-to-face semesters due to COVID-19, although instructors applying flipped learning to their classes also have changed it into non-face-to-face ways, there is still a lack of exploratory research on effectiveness of the new form of flipped learning. In this study, we explored the effectiveness of the non-face-to-face flipped learning by analyzing students' learning experiences throughout FGI and survey. By doing so, we sought to provide in-depth insights for successful implications of non-face-to-face flipped learning classes ultimately. The findings showed that many learners positively evaluated non-face-to-face flipped learning in terms of interactions, including quizzes, team activities, and interpersonal interactions (e.g., Q&A, feedback) with professors in non-face-to-face flipped learning classes. The result of the survey also showed significant differences in the pre-post test regarding learner's perceived learning achievement. Based on these findings, the implications were discussed.

• The Journal of Korean Philosophical History
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• no.31
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• pp.173-202
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• 2011

Hierophany, according to Mircea Eliade, is a natural manifestation of the initial sacred. Through hierophany, profane objects and sites transform their qualities and then they themselves become sacred. People in traditional societies, in an attempt to reenact the initial hierophany, tried to perform consecrating rituals to replicate the first moment of the initial hierophany, at the same time and site. This article focuses on the concrete sites of hierophany. In ancient China, we can see specific sites that manifest the sacred such as Kunlun(崑崙), Jianmu(建木) and zongmiao(宗廟). Kunlun has the characteristics as the Cosmic Mountain that is the realm of the gods that tiandi(天帝) built as his center on the Earth, and it bridges Heaven and Earth. Jianmu joins Heaven, Earth, and the underworld like a Cosmic Tree or Pillar. It can be stated that zongmiao, the royal ancestral temple, functions as the Center of the World, the axis mundi, in which religious human beings express their desire to live in a sacred site, and in which they can regain their initial purity of spirits by communicating with the gods and ancestors. These three are the sites of manifestation of the sacred in China.

• KSBB Journal
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• v.18 no.3
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• pp.211-216
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• 2003

The removal yield of dissolved organic matter in drinking water by biological activated carbon (BAC) process was investigated. The tested processes wer raw water-AC process (BAC1), raw water-ozonation-BAC process (BAC2), and raw water-ozonation-coagulation/sedimentation-BAC process (BAC3). The amounts of organic matter was measured as dissolved organic carbon (DOC), ulta-violet radiation at 254 nm wavelength ($UV_{254}$), total nitrogen (T-N), ammonia nitrogen (NH_3$-N), and total phosphate (T-P). As a results, 30.7% DOC was removed by BAC2 process, which showed higher removal efficiency than BAC1 or BAC3 processes. The removal yield of $UV_{254}$ in BAC1, BAC2, and BAC3 processes were observed as 45.3%, 44.6%, 58.4%, respectively. And the removal yield of ammonia nitrogen were 66%, 81%, 29% in each BAC processes. The optimal empty bed contact time (EBCT) of BAC processes was estimated as 10 minute. This study has shown that BAC process combined with ozone treatment was efficient for removing dissolved organic matter in water.

• Analytical Science and Technology
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• v.16 no.4
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• pp.292-298
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• 2003

In this study, the photooxidation and ozonation of humic acid (HA) in aqueous solution were conducted and the treated HA samples at different reaction time were analyzed using ultrafiltration techniques to evaluate the change of their molecular size distributions with its DOC removal. Molecular size distribution of untreated HA showed 41.5% in higher molecular size fractions (>30,000 daltons) and 15.2% in much smaller molecular size fraction (<500 daltons). As UV irradiation time was increased, it was observed that the degradation of the large molecules of the fraction of >30,000 daltons into much smaller molecules was increased. In UV system, the HA molecules of the fraction of <500 daltons became significantly more and its percentage was increased from 35.3% (UV only irradiation) to 58.9% ($UV/TiO_2$) and 87.8% ($UV/H_2O_2$) in the presence of the photocatalysis. Otherwise, ozonation of HA produced mainly the fraction of medium molecular size ranging from 3,000 to 30,000 daltons with much lower portion (<~7%) in the fraction of <500 daltons. In ozone only system, the fraction of 30,000~10,000 daltons occupied in 41.5% at 60 min of ozonation time. In $O_3/H_2O_2$ system, the fraction of 30,000~10,000 daltons and 10,000~3,000 daltons occupied in 38.9% and 36.2% respectively. Based on these results, we suggested applicable treatment process which could be combined with $UV/H_2O_2$, $UV/TiO_2$ and $O_3$, $O_3/H_2O_2$ system for more effective removal of humic acid in water treatment.