• Korean Journal of Food Science and Technology
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• v.48 no.3
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• pp.268-274
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• 2016

The purpose of this study was to investigate the immuno-stimulatory activity of the high-molecular-weight fraction (HMWF) of Cynanchum wilfordii (CW) extracts obtained by ultrafiltration in murine macrophage RAW 264.7 cells and to assess its immuno-stimulatory effect in mice. Ultrafiltration was performed with polyethersulfone membranes (30 kDa cutoff) in a cross-flow filtration system to obtain the HMWF of CW. The results showed that the HMWF increased the production of various cytokines such as tumor necrosis factor-${\alpha}$, interleukin-6, and nitric oxide in dose-ependent manners. In addition, HMWF treatment increased the relative spleen weight as well as splenocyte proliferation induced by concanavalin A or bacterial lipopolysaccharide in mice. Natural killer (NK) cell activity in the HMWF-treated group was significantly increased compared to that in the control group. These results suggest that the HMWF of CW can support the immune system through secretion of macrophage cytokines, thereby enhancing NK cell activity and murine splenocyte proliferation.

• Journal of Bio-Environment Control
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• v.22 no.4
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• pp.421-426
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• 2013

This study was conducted to investigate the effects of timing of nutrient starvation during transplant production on growth of runner plants and yield of strawberry 'Seolhyang' (Fragaria ${\times}$ ananassa). Nutrient solution supply at the level of EC (electrical conductivity) 0.8 $dS{\cdot}m^{-1}$ was terminated at interval about 10 days between July 25 and September 5. As a result, the growth of above-ground part was inhibited while root growth increased when the nutrient starvation treatment had been brought forward to July 25. It also reduced the T/R ratio significantly and chlorophyll content was tended to be lower than the other treatment. In addition, it significantly promoted the budding, flowering and harvest of first flower cluster. On the other hand, the period of harvest was delayed more than two weeks when the nutrients were continuously supplied after the middle of August. An accumulated marketable fruit yield per plant until the end of January and February was 169 and 266g, respectively in the treatment of nutrient starvation on July 25, which was 71 and 12% increase, respectively, as compared with those in the treatment of September 5. Therefore, the appropriate nutrient starvation in the late season of strawberry nursery period could be expected the increase in yield and income during the winter season by promoting the flower bud differentiation as reducing the endogenous nitrate level of the plantlet.

• Journal of the Korea Organic Resources Recycling Association
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• v.26 no.2
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• pp.11-18
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• 2018

Biochar is a carbon-rich solid product obtained by the pyrolysis of biomass. It has been suggested to mitigate climate change through increased carbon storage and reduction of greenhouse gas emission. The objective of this study was to evaluate carbon dioxide ($CO_2$) and nitrous oxide ($N_2O$) emissions from soil after various biochars addition. The biochars were produced by pyrolysing pear branch, rice hull and bean straw at $400{\sim}500^{\circ}C$. The treatments were consisted of a control without input of biochar and three type biochars input as 5.0 Mg/ha. Emissions of $CO_2$ and $N_2O$ from upland soil were determined using closed chamber for 8 weeks at $25^{\circ}C$ of incubation temperature. It was shown that the cumulative $CO_2$ were 207.1 to $255.2g\;CO_2/m^2$ for biochar input treatments and $258.6g\;CO_2/m^2$ for the control after experimental periods. The cumulative $CO_2$ emission was slightly decreased in biochar input treatment compared to the control. It was appeared that cumulative $N_2O$ emissions were $2,890.6mg\;N_2O/m^2$ for control, 379.7 to $525.2mg\;N_2O/m^2$ for biochar input treatment at the end of experiment. All biochar treatments were found to significantly reduce $N_2O$ emission by 82~87%. Consequently the biochar from byproducts such as pear branch, rice hull and bean straw could suppress the soil $N_2O$ emission. The results from the study imply that biochar can be utilized to reduce greenhouse gas emission from the upland field.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.4
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• pp.323-330
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• 2008

This study was carried out to evaluate the effect of application of cattle manure and chemical fertilizer on dry matter productivity and nutritive values of rye and hairy vetch according to two different sowing methods such as single or mixed-sowed cultivation. Dry matter and TDN yields for rye single culture, and rye and hairy vetch mixed culture were 7.2 and 4.0 ton/ha, and 8.0 and 4.4 ton/ha, respectively. They were significantly (p<0.05) higher than that (4.5 and 2.7 ton/ha) of hairy vetch single culture. Moreover, plots produced by mixed-sowing cultivation had a higher crude protein (CP) as 7.7% than that of only rye plot, and higher TDN and RFV as 55.8 and 79.4%, respectively. Within rye alone plots, completely cattle slurry application produced significantly higher annual DM and TDN yields (7.4 and 4.1 ton/ha) than those of P+K fertilization (7.2 and 3.9 ton/ha) as a chemical fertilizer and non-fertilizer (5.5 and 3.1 ton/ha). Cattle slurry application plot revealed 75.52 and 78.97% of N+P+K fertilization plot for annual DM and TDN, respectively. Within mixed-sowing cultivation with rye and hairy vetch, completely cattle slurry application produced 7.6 ton/ha DM and 4.5 ton/ha TDN, showing 79.50 and 86.77% of N+P+K fertilization plot, respectively. Furthermore, the CP content for cattle slurry plots was 8.5%, which was significantly (p<0.05) higher than those of other plots, and it was also highest in TDN (58.3%) and RFV (86). Overall, rye mixed-sowing with hairy vetch rather than rye alone sowing reduced DM yields, but leaded to enhancement of feed value and nutritive yields. And also, it would be expected that cattle slurry application in comparison with chemical fertilizers might result in reutilization of resources, improved forage quality and, extended harvest period by preventing feed value from rapidly deteriorating.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.2
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• pp.109-117
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• 1986

For the purpose of obtaining basic data which can be applied to processing of retort pouched shellfishes, retort pouched seasoned ark shell, Anadara broughtonii, was prepared. The frozen ark shell was thawed and seasoned with a mixed seasoning powder prepared with $10.0\%$ of sorbitol, $2.0\%$ of table salt and $0.5\%$ of monosodium glutamate at $5^{\circ}C$ for 10 hours, and then dried at $45^{\circ}C$ for 4 hours. The dried seasoned ark shell was coated with $1.0\%$ sodium alginate solution, dried with cola air blast for 2 hours and then vacuum-packed in the laminated plastic film bag (polyester/casted polypropylene= $12{\mu}m/70{\mu}m,\;15{\times}16cm$), and finally sterilized up to Fo=6.0 in hot water circulating retort at $121^{\circ}C$ for 10 minutes. The major fatty acids of raw ark shell and retort pouched seasoned ark shell products were 16:0, 20:5, 22:6, 18:0 and 18:3, and predominant free amino acids of those were lysine, arginine, glycine, alanine, glutamic acid and leucine. In nucleotides and its related compounds of raw ark shell and retort pouched seasoned ark shell products, the most abundant one was AMP, and total extract-N of those was chiefly consisted of free amino acids, betaine and nucleotide and its related compounds. During the processing procedure such as drying and sterilization, unsaturated fatty acids slightly decreased while saturated fatty acids increased, and total extract-N content decreased about a half. From the results of chemical and microbial experiments during storage, it was concluded that the products could be preserved in a good condition for 100 days at room temperature, and their duality could be improved by the coating treatment of sodium alginate solution.

• Korean Journal of Soil Science and Fertilizer
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• v.2 no.1
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• pp.31-38
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• 1969

This experiment was carried out to determine the effect of different levels of phosphate and potash firtilizers applied on yields and quality of leaf tobacco. It was done at all varying levels of phosphate and potassium fertilizers $S_0$ (non-fertilizer plot);N:P:K=0:0:0 (Kg/10a) $S_1$ (1/2 amount plot);N:P:K=10:7.5:10 (Kg/10a) $S_2$ (standard plot);N:P:K=10:15:20 (Kg/10a) $S_3$ (1.5 times plot);N:P:K=10:22.5:30(Kg/10a) $S_4$ (2.0times plot);N:P:K==10:30:40 (Kg/10a) Urea was applied as nitrogen, triple superphosphate as phosphate, potassium sulfate as potash source. The total amount of fertilizers was applied as basal dressing per one plant. This experiment was carried out by using randomized block design with 3 replication. The results obtained in terms of yields and quality are summarized as follows. 1. By increasing the amounts of phosphate and potassium fertilizers, growing status of the largest leaf length, the largest leaf width and stem size of tobacco increased in the order of $S_4>S_3>S_2>S_1>S_0$. 2. By increasing the amount of phosphate and potassium fertilizers, yield was increased in the order of $S_4>S_3>S_2>S_1>S_0$, This increase showed generally high significance among various levels of treatments. 3. The regressive equation was derived from the relationship between treatment levels and tobacco leaf yield. The most desirable treatment level for the maximum yield was estimated as the 2.87 times as much as the treatment level of phosphate and potasium fertilizers of standard plot. 4. By increased application of phosphate and potash fertilizers, the contents of $P_2O_5$ and $K_2O$ in the tobacco leaf became greater while the content of nicotine was decreased. Thus, a negative correlation between fertilizer application and nicotine content has been observed.

• Journal of Animal Science and Technology
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• v.45 no.4
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• pp.551-558
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• 2003

This study was conducted to investigate the effect of dietary wormwood powder (Artemisia montana Pampan; WP) supplementation on growing performance and fecal noxious gas emission in weanling pigs. One hundred and twelve pigs were alloted into four treatments and offered for 4 weeks one of the diets supplemented with 0% (control), 0.5% (0.5), 1.0% (1.0) and 2.0% (2.0) WP. Each treatments has four replicate with 7 pigs per replicate. ADG, ADFI and F/G were improved by 1.0 and 2.0 WP supplementation during 0d${\sim}$14d feeding. Feed intake of 1.0 WP diet was higher (P<0.05) than any other diet during 15d${\sim}$28d feeding. But there were no differences among the other treatments in the weight gain and feed conversion. During the whole feeding period, daily weight gain and feed intake of pigs fed 1.0 and 2.0 treatments were higher than those of pigs fed control and 0.5 diet. DM digestibility was significantly (P<0.05) higher in weanling pigs fed 0.5, 1.0 and 2.0 WP than that of the control. The protein digestibility was significantly improved in 1.0 WP treatment and the phosphorus digestibility was improved in 2.0 WP treatment. Excretion of nitrogen and phosphorus was significantly (P<0.05) decreased by 2.0 treatment than control. Emission of fecal ammonia after 48 hours storage in vinyl bag dramatically decreased by all the treatments except control. However, there was no statistical difference in fecal hydrogen sulfide emission among treatments. In conclusion, this study suggested that the dietary 1.0${\sim}$2.0 supplementation of WP can improve productivity of pigs decrease fecal $NH_3$ and $H_2S$ gas emission.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.1
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• pp.13-22
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• 2003

This pot experiment was conducted to find out the forage productivity and mineral contents in an orchardgrass sward affected by the compost, lime, and phosphorus applications on the newly reclaimed and arable pasture soils. This 3rd part was concerned with the effect of phosphorus application($P_{0}$ : control, $P_1$=1.25ｇ, $P_2$=2.50g, $P_3$=3.75g $P_2$$O_{5}$ pot). The results obtained are summarized as fellows; There were considerable differences between the newly reclaimed and arable pasture soils in the soil chemical characteristics as follows in general; poor~common in the newly reclaimed soil and common~good in the arable pasture soil in terms of soil fertility standard. Comparing with the arable pasture soil, the contents of available$ P_2$$O_{5}$ / in the newly reclaimed soil at end of experiment were considerably increased by the $P_2$$O_{5}$ applications, These effects in the newly reclaimed soil were positively influenced by the additional application of compost, but were adversely influenced by the additional application of lime. The seedling vigour and yield of orchardgrass were far better in the arable pasture soil than in the newly reclaimed soil. At the $P_{0}$ treatment, those were very poor in 也e newly reclaimed soil, but were good in the arable pasture soil. Comparing with $P_1$ treatment, the above effects of heavy $P_2$$O_{5}$ / applications($P_2$and $P_3$) were not recognized. On the newly reclaimed soil, the above effects were markedly enhanced by the applications of $P_2$$O_{5}$ with compost. The Ca, Mg, and P contents of orchardgrass were relatively higher on the arable pasture soil than on the newly reclaimed soil, whereas ere were no differences in the K contents.

• Journal of Animal Science and Technology
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• v.44 no.5
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• pp.549-560
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• 2002

This study, including two in vitro experiments and an in vivo experiment were conducted to evaluate effects of Passtein$^{(R)}$ on crude protein degradability, ruminal fermentation characteristics and nutrient digestibility. In in vitro experiment protein degradability was examined using borate-phosphate buffer and neutral detergent, and using protease from Stroptomyces griseus at 39$^{\circ}C$ for 0, 2, 4, 8, 12, and 48 h. In addition, an in vivo experiment was conducted in a switch back design and ruminal fermentation and nutrient digestibility were determined. Four ruminal-fistulated Holstein cows weighing 300kg in mean body weight randomly allotted to 2 treatments (control and Passtein$^{(R)}$ supplementation). Although there was no significant difference on protein fraction between treatments, it appears that Passtein$^{(R)}$ supplementation decreased buffer soluble protein fraction compared to control. Protein degradability was not affected by Passtein$^{(R)}$ from 0 h to 4 h, but decreased at 12 h and 48 h compared to control. Degradation of immediately degradable fraction was higher in Passtein$^{(R)}$ treatment, but degradation of fermentable fraction was lower in Passtein$^{(R)}$ treatment compared to control. The pH and $NH_3$-N concentration tended to increase in Passtein$^{(R)}$ treatment, but VFA production, microbial counts and enzyme activity tended to decrease in Passtein$^{(R)}$ treatment compared to control. In addition, nutrient digestibility in the total tract tended to increase in Passtein$^{(R)}$ treatment compared to control.

• Journal of Nutrition and Health
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• v.48 no.2
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• pp.157-166
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• 2015

Purpose: The effects of water extract and distillate from the mixture of black goat meat and medicinal herb on MG-63 osteoblast proliferation and mouse bone marrow derived osteoclast formation were investigated. Methods: Proximate composition, volatile basic nitrogen (VBN), mineral content, free amino acid composition and free fatty acid composition in black goat meat were determined. Water extract and distillate were prepared with three groups; goat meat only (BG-E, BG-D), six herbs added group (BG-E6, BG-D6), and eight herbs added group (BG-E8, BG-D8). Osteoblast proliferation, mineralization and calcium uptake activity of MG-63 cells were measured and tartrate resistant acid phosphatase activity of osteoclasts was analyzed. Results: Black goat meat had remarkably low fat and high level of calcium. Glutamic acid was the most abundant amino acid. Herbs added extract groups (BG-E6 and BG-E8) showed increased MG-63 cell proliferation in a concentration dependent manner, while all the distillates did not show the effect. All extracts and distillates showed significantly increased osteoblast mineralization depending on the concentration. In particular, herb added extract, BG-E6, increased 170.3% of control and the distillate of BG-D and BG-D6 increased up to 168.5% and 159.8%, respectively. Calcium uptake activities of all water extracts showed remarkable increase of BG-E6 and BG-E8 up to 615.5% and 628.1% of control, respectively. Ditillates had no effect except BG-D6. All water extracts significantly reduced the activity of tartrate-resistant acid phosphatase (TRAP) in osteoclasts derived from mouse bone marrow. Conclusion: Combination of black goat meat and medicinal herb increased the MG-63 cell proliferation and effectively inhibited osteoclast differentiation in both water extracts and distillate of them, which implies that they could be used as potent functional food materials for bone health.