• Journal of Marine Life Science
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• v.3 no.2
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• pp.59-66
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• 2018

The assessment of level of health of the tidal flats can be evaluate by health of organisms inhabit the tidal flats. It is possible to evaluate the precise health level of organisms inhabit the tidal flats using analysis of expression of biomarker genes. The purpose of this research is to evaluate the health of the tidal flats on the west coast using biomarker genes such as heat shock protein 70 (Hsp70), heat shock protein 90 (Hsp90), glutathione S-transferases (GST) and thioredoxin (TRX). These genes are stress, immune, and antioxidant related genes that can be used to look at the health of an organism through gene expression. In this study, we collected manila clam (Ruditapes philippinarum) in 8 analysis areas on the west coast. Expression of the genes was analyzed by RT-qPCR method. Results showed that, the expression of Hsp70, Hsp90, GST and TRX genes were differentially expressed in the 8 analysis areas. In particular, the expression of Hsp90 and GST or the expression of Hsp70 and TRX were similar. This means that there is a substance that reacts specifically to each gene. Therefore, I think suggest that the based on the results of physicochemical analysis, it can be selected genes suitable for analysis. These results suggest that Hsp70, Hsp90, GST and TRX were played roles in biomarker for assessment of the health of tidal flats.

• Journal of Wetlands Research
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• v.11 no.3
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• pp.1-8
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• 2009

The groundwater dependent ecosystem associated with a natural stream is the area where mixing and exchange of surface water and groundwater occurs due to large chemical and hydraulic gradients. Surface-groundwater interactions play an important role in biogeochemical processes in groundwater dependent ecosystems and make this area a hydro-ecological hot spot. The objective of this study is to characterize the groundwater dependent ecosystem in a natural stream where nitrate contamination of stream water is observed by means of hydrogeological, chemical, and biological methods. In this study, vertical flow exchange(hyporheic flow) rates between stream and groundwater were estimated based on vertical hydraulic gradients measured at mini-piezometers of various depths. To investigate the biological natural attenuation potential, biological analyses using polymerase chain reaction(PCR)-cloning methods were performed in this study. Results show that the veritical hyporheic water fluxes affect nitrate concentrations and bacterial densities in groundwater dependent ecosystems to some degree. Also, denitrifying bacteria were identified in hyporheic soils, which may support the biodegradation potential of the groundwater dependent ecosystems under certain conditions.

• Microbiology and Biotechnology Letters
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• v.23 no.3
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• pp.365-371
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• 1995

Filamentous bacteria and other large microorganisms, which are useful indicators of bulking and poeration, were identified by microscopic observation. Activated sludge samples were taken from the aeration basin of 5 municipal wasterwater plants and 6 industrial ones. Among the filamentous bacteria founds, Microthrix parvicella was most frequently present, followed by type 0041, type 1701 and Nocardia. This frequency of occurrence was similar to those reorted in USA and the Neterland. The morphology of filamentous bacteria observed were generally identical with those previously reported. except type 1701 which had slender filament diameter. Among protozoa, Vorticella was most frequently present, followed by Aspidisca, Opercularia and Difflugia. Philodina was the only metazoa observed. Both filamentous bacteria and protozoa would be useful indicator organisms. The potential for these organisms as indicators were discussed.

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.170-170
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• 2021

본 연구에서는 토지이용변화 예측 모형으로 산출된 토지이용도를 사용하여 용담댐 소유역의 지표유출량을 비교 및 분석하였다. 토지이용예측모형은 DynaCLUE 모형을 사용하였으며, 토지이용 면적 시나리오는 2000년, 2007년 및 2013년 실제 중분류 토지이용도를 기반으로 회귀식을 산정하였다. 모의된 토지이용도는 실제 토지이용도와 공간적인 분포 및 면적 비교를 통해 변환 탄성계수와 변환 행렬을 수정하여 검·보정하였다. DynaCLUE 모형으로 모의된 토지이용도는 공간적인 분포에서 초지가 실제 토지이용도와 차이가 발생하였으나, 각 토지이용별 면적을 비교한 경우 모의 토지이용도와 실제 토지이용도가 매우 유사하게 나타났다. CLUE 모형으로 모의된 토지이용도에서 발생하는 공간적인 불확실성은 복잡한 용담댐 소유역의 토지이용을 반영할 Driving factor가 부족하여 발생하는 것으로 판단된다. 산출된 모의 토지이용도를 SWAT 모형의 입력 자료로 사용하여 2013년 용담댐의 소유역 지표유출량을 모의하였다. SWAT으로 산정된 유출량의 보정은 SWAT-CUP의 SUFI-2 알고리즘을 이용했으며, 보정된 모의 지표유출량과 실제 유량 측정값을 비교한 결과 유의미한 비교 결과가 나타났다. 향후 토지이용예측모형을 이용하여 토지이용 변화를 수문 분석에 반영하는 추가 연구가 필요할 것으로 판단된다.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.833-834
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• 2001

A membrane immuno-chromatographic system that selectively separated plasma lipoproteins and generated a signal in proportion to the concentration of cholesterol within high-density lipoprotein (HDL) was investigated as a point-of-care device for the prognosis of coronary heart disease.

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2004.05a
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• pp.36-36
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• 2004

• Korean Journal of Environment and Ecology
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• v.28 no.4
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• pp.472-483
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• 2014

Major cities have strengthened their efforts to improve the environmental function of the urban forest through aspects of quantity and quality. Yet, it is difficult to propose both long-term improvement and management policy direction continuously due to a lack of evaluating method that social needs could be reflected. After all, effective strengthening of the function of an urban forest, composed of social and biotic environments, is essential to create evaluation criteria. Such data reflects a quantity variation and identifies the distribution and habitation of biotic sources as well. An assessment of utilization possibilities presents such values as a green connectivity, an ecological healthiness, and usage and access opportunities. Consequently, this research was executed to create an urban forest sustainability index that proposes a management and policy direction based on the evaluation of quantity and quality aspects. This study was processed by the following steps: a degree of importance of ecological aspect, a sub-classification index choice, and an examination of adequacy. This research was conducted from premises that urban forest had another functions comparisons to a natural forest. As a result, a criteria of sustainability should be considered to evaluate an ecological aspect, as well as the social and management aspects regarding human disturbance. This research is designed to develop an evaluation index for ecological aspect. In order to evaluate the ecological healthiness of an urban forest, a vegetation index considers aspects of related rarity, damage possibility, naturalness, area, and diversity. In a wildbird index-related study, forest duration and area were selected. Finally, a variance analysis is presented, inclusive of stability, naturalness, diversity, potentiality, and possibility of wildlife inhabitation, which is indicative of an urban forest sustainability index.

• Journal of Korean Society of Forest Science
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• v.87 no.3
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• pp.347-357
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• 1998

The objective of this study was to present the proper criteria which could be efficiently used for dividing the land-use zones in Korean national parks. Delphi technique was employed to collect data for this study. The Delphi process was designed with 3 round questionaries for Korean panel experts. A list of 30 criteria was obtained to be considered in dividing land-use zones. From the results, the biological category, in which 10 criteria were involved, occupied the most part of them. This means that the panels consider the biological criteria to be the most important ones under the serious situation of environmental deterioration. Using the 30 criteria emerged from this study, it could be possible to analyze the fitness of the existing land-use zoning system. Prior to the application of these criteria to each park, however, the areal characteristics should be surveyed to select the proper criteria. The new zoning system based on the regional characteristics of each park could be efficiently utilized for management of Korean national parks.

• Proceedings of the Korean Society of Computer Information Conference
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• 2022.01a
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• pp.153-154
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• 2022

본 논문에서는 역연령으로 구분되었던 기존 생물학적 성숙도에 기반한 체력 지표가 아닌 골성숙도를 활용한 생물학적 성숙도에 기반하여 유소년의 신체에 맞는 체계적인 운동을 추천하는 기법을 제안한다. 이를 통해 유소년의 성장기에 개인화된 운동능력 발달을 성취하게 함으로써 국민 체력 증진에 기여하고 체육 공교육 활성화 및 유소년 피트니스 관리 산업 발전에 도움이 될 것으로 기대한다.

• Clinical and Experimental Reproductive Medicine
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• v.16 no.2
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• pp.119-130
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• 1989

본 연구는 생식주기중 폐쇄여포액내에서 생물학적， 면역학적 특성을 나타내는 GTH 의 변화를 조사하고 steroid hormone과의 상관관계를 조사하며 국부조절인자로서 의 GTH의 역 활을 조사하고자 하였다. 가임기간중 215개의 여포와 IVF과정에서 185개의 여포를 얻어 여포액내 GTH의 생물학적 또는 면역학적 활성을 측정하였다. Bioactive LH(bLH)는 생쥐의 Leydig cell-testosterone production assay, bFSH는 흰쥐의 Sertoli cell aromatase assay로 측정 하였 다. Immunological GTH(iLH , iFSH) 는 MaiaClone RIA , Delfia kits를 사용하였다. 여포액내 iLH, iFSH , ihCG 는 hyperstimulation에 의해 형성된 여포의 크기와는 무관하였다. 또 hMG, huFSH 의 처리와도 상관성이 없었다. T의 농도가 높은 여포액내의 iFSH는 현저히 낮았으며 E, P 가 고농도인 여포의 ihCG 양은 현저히 낮았다. 과배란이 유도된 난소의 여포액내 iLH는 LH specific RIA로 측정시 3mIU/ml 이하이었다. 생식주기중 여포액내 bLH, bFSH는 배란기에 현저히 증가 하였다. 혈청내 GTH B/I ratio는 엘정한 반면 여포액내 LH，FSH의 생물학적， 면역학적 활성은 미수정란을 가지거나 폐쇄된 여포내의 활성보다 현저하게 높았다. 위의 결과로 보아 여포액내 생식소자극호르온은 면역학적활성보다 높은 생물학적 활성을 가지며， 생리적 현상의 지표가 된다고 추론된다. 또한 steroid, bGTH는 여포의 선택， 폐쇄를 구분하는 지표로 사용가능하며， 여포가 폐쇄될때 여포액내 B/I ratio가 현저히 낮아지는 것으로 보아 GTH의 활성이 감소되는 것으로 판단된다.