• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1992.05a
• /
• pp.35-35
• /
• 1992

산소라디칼-질병모델을 보다 간편한 방법으로 만들어 생화학적 발병 기전을 추구하므로서 신약창출에 필요한 기초자료를 제시하고자 본 실험을 실시하였다. 미세혈관의 혈류이상은 과잉의 활성산소를 생산하게되며 이로 인해 여러 종류의 난치성 질환 및 염증을 수반하는 많은 질병이 발병하게 된다. 일반적으로 흔히 이용되어지는 허혈-재관류모델이 아닌 환경독성물질을 활용한 병태모델을 만들어 이 모델조건에서 활성산소생성과 밀접한 관계를 갖는 잔틴 옥시데이즈(Xanthine Oxidase)의 변화와 과산화지질생성정도를 연관지어 검토하였다. 수은, 구리, 몰리브덴등의 금속이온들은 잔틴 디하이드로저네이즈(Xanthine dehydrogenase)로 부터 옥시데이즈(Oxidase)형으로의 전환을 촉진시켰으며 막독성의 지표로 이용되어지는 과산화지질의 생성도 현저히 증가시켰다. 또한 알데하이드류의 첨가실험에서도 잔틴산화효소의 형전환이 촉진되었으며 첨가한 알데하이드의 탄소수와 수소수 그리고 탄소와 탄소의 결합상태와도 밀접한 관계가 있음이 관찰되었다.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.19 no.11
• /
• pp.1029-1032
• /
• 2006

Monolayers of lipids on a water surface have attracted much interest as models of biological membranes, but also as precursors of multilayer systems promising many technical applications. They exhibit very interesting physico-chemical properties as two-dimensional and interfacial systems. Until now, many potential methodologies have been developed in order to gain a better understanding of the relationship between the structure and function of the monolayers.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.11 no.8
• /
• pp.628-636
• /
• 1998

LB layers L-$\alpha$-DLPC deposited by LB method were deposited onto n-type silicon wafer as Z-type film. Films made up of 8, 16 layers of lipid with long alkyl chain and the thickness of monolayer and multiayers was determined by ellipsometry. Ut was deposited Ag and Al onto LB layers and silicon wafer for electrode and small electrode exhibit high capacitiance and low lekage current. The C-V curves of the MLS capacitor shows very high saturation value of capacitance. And cross-sectional SEM image of MLD capacitor indicated the presence of pore with Al electrode and we found that the Ag is good for electrode metal.

• Electrical & Electronic Materials
• /
• v.9 no.7
• /
• pp.696-701
• /
• 1996

In this study, deposited lipid membranes on the electrode and detected thermally stimulated displacement current generated from it. The researchers examined displacement current of electric conduction organic monolayer generated due to orient change of monolayers alkylchain and changed of dipole moment vertical component due to thermally stimulated. We paid attention to the phase transition temperature obtained by the thermally stimulated displacement current of lipid membrane layers this time. We detected the thermally stimulated displacement current peak of layers. From above results the transition temperature dilauroylphosphatidylcholine layers is about 43.deg. C. This study also compared above results with those obtained by differential thermal analysis method.

• The Korean Journal of Malacology
• /
• v.22 no.1 s.35
• /
• pp.27-38
• /
• 2006

We investigated the reproductive cycle with gonad developmental phases of Solen grandis by histological observations. Seasonal changes in biochemical components of the adductor muscle, visceral mass, foot muscle and mantle were studied by biochemical analysis, from January to December, 2005. The reproductive cycle of this species can be classified into five successive stages: early active stage (December to January), late active stage (January to March), ripe stage (March to July), partially spawned stage (June to July) and spent/inactive stage (July to December). Total protein content was the highest in the foot muscle, the content was high in January (early active stage), the lowest in April (ripe stage), and was the highest in August (partially spawned stage). In the visceral mass, total protein content began to increase in February (late active stage) and reached a maximum in March (ripe stage). Thereafter, it gradually decreased between June and July (partially spawned stage). There was a strong negative correlation in total protein contents between visceral mass and mantle (r = -0.594, p = 0.042). Meanwhile there was a positive correlation between the adductor muscle and foot muscle, the correlation was not statistically significant (r = 0.507, p = 0.093). Total lipid content was the highest in the visceral mass; it was more than 2 to 5-fold higher than that in the adductor muscle, foot muscle, and mantle. Monthly changes in total lipid content were also most dynamic in the visceral mass. It was relatively higher between January and February, showed a maximum in March (the ripe stage), decreased rapidly from April to July (ripe and partially spawned stage), and gradually decreased from September to December (spent/inactive stage). There was a strong positive correlation in total lipid content between foot muscle and adductor muscle (r = 0.639, p = 0.025). Tthough a negative correlation was found between visceral mass and mantle (r = -0.392), the correlation was not statistically significant (p = 0.208). Glycogen contents changed within relatively narrow range and were similar among different tissues. There was no statistically significant correlation in glycogen contents among tissues.

• The Journal of Korean Medicine
• /
• v.22 no.2
• /
• pp.53-63
• /
• 2001

This study was carried out to investigate the physiological activityof the water extract from EA. The present study was done to investigate the effects of EA on cultured hepatocyte cell system and lipid peroxidation in $A{\beta}$ treatment conditions. Pretreatment of EA attenuated in cell cytotoxicity enhanced by increasing concentrations of $A{\beta}$. MDA level induced by $A{\beta}$ treatment was significantly increased and the level was slightly reduced by pretreatment of EA. The ability of EA to reduce cell death and MDA level induced by $A{\beta}$ suggest that EA may be a protective agent against free radical generating compounds such as $A{\beta}$. EA exhibited anti oxidative activity at all concentration tested.The extract was as good as antioxidative activity of the synthetic antioxidants, butylated hydroxy toluene and ascorbic acid. Furthermore, this was superior to that of natural antioxidant, a-tocopherol. In the presence of heavy metal ions ($Fe^{2+},{\;}Zn^{2+}$), EA showed strong antioxidative activity. The extracts showed about 3075% in the nitrite scavenging effect under pH 1.2 and $37^{\circ}C$ for 1 hr. There was significant difference among concentration of extracts.

• Journal of Life Science
• /
• v.13 no.2
• /
• pp.168-174
• /
• 2003

The chemical compositions and antioxidant activity of green tea extracts in medilite-extraction water were compared to that of distilled water(DW). Antioxidant activity was fetermined by the formation of thiobarbituric acid reactive substances (TBARS) in rat liver homogenates and microsomes and the scavenging activity of free radicals by DPPH ($\alpha$, $\alpha$'-diphenyl-$\beta$-picrylhydrazyl). The order of total polyphenolic compounds and extracted yield by extracts was medilite 325 mesh-extraction water, medilite 600 mesh-extraction water and distilled water(DW). The ranges of scavenging activity of green tea extracts in DPPH method were 60.95% - 64.51%. The inhibition ratios of TBARS formation in the rat liver homogenates and microsomal fractions were significantly lower with green tea extracts by DW-extraction than with both medilite 325 mesh and 600 mesh-extraction water. The concentration of iron ion of water containing medilite 325 mesh and green tea extracts and of water containing medilite 600 mesh and green tea extracts were significantly higher compared to DW. Therefore, this result suggested that enhanced concentration of iron ion in green tea extracts by medilite-extraction water containing high iron ion content was associated with enhanced peroxidation of the rat liver microsomal fractions. These results showed that total polyphenolic compounds, the % of yield and mineral compounds of green tea extracts were increased using medilite 325 mesh and 600 mesh-extraction water.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1996.04a
• /
• pp.231-231
• /
• 1996

Paraquat(1.1'-dimethyl-4,4'-bipyridinium ion, PQ)는 전세계적으로 가장 많이 사용되고 있는 농약으로 자살 또는 실수로 마시는 경우 예외없이 폐독성으로 사망하게 된다. 하지만 아직까지 임상에 사용되어지고 있는 효과적인 독성 경감제는 전무한 실정이다. 현재까지 밝혀진 paraquat의 주된 독성기전은 NADPH Cytochrome P$_{450}$ reductase에 의해 산화,환원 반응을 거치는 동안 free radical을 생성하여 세포막에 지질과산화를 일으켜서 세포막의 기능상실과 cell death를 일으킨다고 보고되고 있다. 따라서 본 연구에서는 항산화작용이 뛰어난 아미노산인 taurine(TA)의 radical scarvenging 효과에 의한 PQ 독성경감효과를 in vivo에서 검색하였고, in vitro에서 TA의 PQ 독성경감 mechanism을 밝히고자 하였다. in vivo에서 PQ의 간독성(s-GOT,s-GPT), 신장독성(BUN, Creatinine), 폐 및 전신독성(ALP, MDA, G-6-phosphatase)의 정도를 혈액 및 조직균질액 중에서 검색함으로써 TA의 독성억제효과를 측정하였다.

• YAKHAK HOEJI
• /
• v.38 no.2
• /
• pp.131-139
• /
• 1994

Calcein-encapsulated small unilamellar vesicles of various lipid composition were prepared using the sonication technique, and their stabilities at $20^{\circ}C$ were examined by measuring calcein leakage from the liposomes. The fluidity of these liposomal bilayers was also investigated by measuring the fluorescence polarization of DPH labelled into the liposomes. The results showed that liposomes made of PC mixtures with different acyl chain length were very stable, which may be due to the formation of interdigitated bilayer structure. The addition of cholesterol further stabilized these PC liposomes. However, addition of cholesterol reduced the encapsulation efficiences of liposomes. The fluidity of the liposomes was significantly decreased by cholesterol in the liquid crystalline state, but not changed in the gel state. These results suggest that the enhanced stability of PC mixture liposomes may be ascribed to the formation of stable interdigitated bilayer structure. In membrane-mimetic and drug-delivery studies, vesicles made of mixtures of various phospholipids are recommended instead of addition of cholesterol to the phospholipid.

• Korean Journal of Food Science and Technology
• /
• v.15 no.2
• /
• pp.195-201
• /
• 1983

Lipids isolated from three barley samples were identified and quantitated by column, thin layer and gas liquid chromatographic techniques. These lipids were shown to consist of 69.3-73.1% neutral lipids, 9.6-16.5% glycolipids, and 14.2-17.9% phospholipids. Among the neutral lipids, triglycerides were predominant (54.2 to 55.7%) with smaller amounts of 1,2-diglycerides, 1,3-diglycerides, free sterols, free fatty acids, steryl esters, and three unknown being present. Among the glycolipids, digalactosyl diglycerides (31.3 to 33.2%) and monogalactosyl diglycerides (26.2 to 29.6%) were the most abundant. Esterified steryl glycosides, steryl glycosides, cerebrosides, sulfolipids, and an unknown component were present as minor components. Of the phosopholipids, phosphatidyl cholines and serines, lysophosphatidyl cholines, and phosphatidyl ethanolamines were the major components, comprising over 80% of this class. The major fatty acids in the total and the three lipid classes were palmitic, oleic, linoleic and linolenic acids. However, the neutral lipids fraction contained more oleic acid than other lipid fractions, and the phospholipids fraction contained more palmitic acid than the other lipid fractions.