• Journal of Periodontal and Implant Science
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• v.30 no.2
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• pp.213-231
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• 2000

이 연구의 목적은 다공성의 CPP 내부에 쥐의 장골의 골수에서 유래된 세포를 접종하고 3차 원적으로 배양하여 CPP가 골 형성을 위한 조직공학의 지지체로 적용가능한가를 연구하는 것과 Calcium PolyPhosphate(CPP)의 돌연변이 유발성을 검사하는 것이다. 무수 ($Ca(H_2PO_4)$)를 condensation하여 무결정의 ($Ca(PO_3)$)를 얻고 이를 용융하고 냉각시킨 후 분쇄하여 Calcium polyphosphate(CPP) powder를 얻었다. 다공성의 CPP는 5% $SiO_2$를 첨가하여 sponge 형태로 $450-550{\mu}m$ 소공의 크기를 가지는 것과(CPP-45ppi) $200-300{\mu}m$의 소공의 크기를 가지는 것(CCP-60ppi) 2가지로 제작하였다. 각각의 CPP matrices는 $5mm{\times}5mm{\times}1mm$의 블록 형태로 만들었다. 체중 100g 내외의 백서에서 장골(femur, tibia)을 채취하여 백서의 장골 골수 세포를 분리하여 배양한 후 24well에 CPP block을 넣고 CPP block 당 $10^5$개의 배양한 세포를 접종하였다. 배양 1, 7, 14, 및 21 일째에 각 well에서 trypsin EDTA를 이용하여 2회 반복하여 cell을 분리하였고, 원심분리한 후 hemacytometer로 측정하였다. 또, 45ppi와 60ppi, 그 리 고 Tissue Culture Polystyrene(control group)에 접종, 배양된 세포들의 염기성 인산분해효소활성도를 배양 7, 14, 및 21 일째에 각각 측정하였다. 각 기간별로 배양된 세포-CPP 혼합체내에서 세포의 부착 및 증식과 형성된 조직의 3차원적 형태를 관찰하기 위하여 주사전자현미경하에서의 관찰하였다. CPP의 돌연변이 유발성 검사 (mutagenicity test)를 위해 hypoxanthine-guanine phosphoribosyl transferase(HPRT) assay를 하였다. NIH3T3 cell line과 CHO-K1 cell line으로 각각 $1000{\mu}g/m{\ell}$, $100{\mu}g/m{\ell}$, $10{\mu}g/m{\ell}$ 그리고 $1{\mu}g/m{\ell}$의 CPP 농도에서 측정하였다. 통계적 분석을 위해서 모든 측정은 각군당 4개체 이상 시험하였고, 각 측정값은 평균값${\pm}$표준편차로 나타내었다. 각 군간의 통계적 유의성 검정을 위해서 Analysis of variance(ANOVA)를 이용하였고 Tukey의 방법으로 사후분석을 실시하였다. 제작된 CPP matrices 소공들이 서로간에 연결이 잘 되어있는 형태였다. 두 가지로 제조된 CPP(45ppi와 60ppi) 모두에서 세포의 부착이 잘 일어났고, 부착된 세포의 분열도 잘 일어났다. 2 가지의 CPP 모두에서 7, 14, 21일째의 세포 수는 1일째에 비해 유의성 있게 증가하였다(P<0.01). 3차원적 구조인 Calcium PolyPhosphate에서 배양한 세포는 24well dish(tissue culture polystyrene)에서 평면적으로 배양한 대조군의 세포에서 보다 염기성 인산분해효소 (Alkaline Phosphatase)를 유의성 있게 높게 나타냈다. 주사전자현미경에서 세포-CPP 혼합체를 관찰한 결과, CPP block에 세포들이 잘부착되어 있었고, 시간이 지남에 따라 세포가 여러 층을 형성하면서 뭉치는 현상을 보였다. 또, HPRT assay 결과 , Calcium PolyPhosphate는 돌연변이 유발성을 보이지 않았다. 이상의 결과로 볼 때 CPP에는 세포부착이 잘 일어나고, 지지체 상에서 세포의 분열도 활발하게 일어나므로 골조직을 위한 조직공학의 우수한 지지체가 될 수 있을 것으로 사료된다.

• Journal of the Korean Geosynthetics Society
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• v.3 no.2
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• pp.3-11
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• 2004

Fabric Foam systems provide a variety of flexible bank protection for open channels and hydrulic structures. The structural performance and durability of conventional bank protection materials such as concrete, gravel, riprap and vegetation can be significantly improved by confining the materials within the cells of Fabric Foam system. This paper presents the results of field and laboratory tests carried out to evaluate the performance of new Fabric Foam System as a Bank Protection. The results of the tests confirmed effect of Fabric Foam System in Bank Protection.

• Journal of the Korean Geosynthetics Society
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• v.4 no.2
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• pp.47-56
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• 2005

Recently, geosynthetic reinforced earth walls are gradually replacing conventional concrete retaining walls for reasons of economy, expediency of construction, and aesthetics. A number of reinforced soil walls having more than 10m heights have been constructed to make more effective development in the country. However, mistakes in design and construction of reinforced earth walls have resulted in many troubles such as failure of reinforced earth walls, horizontal deformationor breakdown of facings, and so forth during or after construction. In this paper, a case study on global sliding failure of a geogrid-reinforced tiered wall is carried out to investigate the causes of the failure and suggest the proper countermeasures. From the subsurface investigation and field instrumentation, It is found that the cause of the global sliding failure was occurred by decreasing of bearing capacity of foundation ground induced by infiltration of rainwater.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2004.05a
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• pp.618-622
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• 2004

The vibration test system of SITC(Satellite Integration and Test Center) at KARI(Korea Aerospace Research Institute) has been used successfully for the environmental tests of a majority of korean space programs, such as KOMPSAT, Koreasat KITSAT, STSAT and KSR program since 1996. To meet the recent needs of large size test facility available for the vibrational tests of the huge launch vehicles and tole-communication satellites which will be developed in the near future, KARI undertook to construct the large size multi-electromagnetic shaking system with 3 $\times$ 3m head expander system. The new system will consist of three electromagnetic shakers which has 160 kN thrust force individually, and be able te sustain up to 8 tons test load and 300 kNm overturing moment. And to avoid the tremendous cost and effort to furnish the seismic block with large size and weight, it will adopt a Lin-E-Air type configuration with which the seismic block is less severe than a Solid-Truninon type. In addition, to fulfill the strong requirement of high overturning moment the additional guidance system including a central bearing system on a central support and several pad bearings around the head expander body is now considered. This paper describes the configuration and the design parameters of the multi-shaking system which is under development by KARI's engineers.

• Journal of Korean Tunnelling and Underground Space Association
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• v.7 no.4
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• pp.305-311
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• 2005

Shotcrete adhesive strength in large section tunnels in jointed rock masses plays an important role in preventing rock block from falling and shotcrete debonding due to blasting vibration. Nevertheless, it has not been considered as a major factor such as shotcrete compressive strength in design and construction. For this reason, the purpose of this study is to analyze the effect on shotcrete adhesive strength for large-sectioned tunnels. First, the parametric study using numerical model similar to Holmgren's punch-loaded test was executed for various range of adhesive strength. It shows that the shotcrete bearing capacity is linearly proportioned to the adhesive strength between shotcrete layer and blocks. And then, distinct element analysis of a jointed rock tunnel for an adhesive strength of 1 MPa and a conventional fully-bonded condition between the shotcrete layer and the excavation face was compared in order to evaluate the effect of the shotcrete adhesive strength.

• Explosives and Blasting
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• v.37 no.4
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• pp.26-35
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• 2019

In designing a gravity-type anchorage of earth-anchored suspension bridge, the contact friction between a blasted rock mass and the concrete anchorage plays a key role in the stability of the entire anchorage. Therefore, it is vital to understand the shear behavior of the interface between the blasted rock mass and concrete. In this study, a portable 3D LiDAR scanner was utilized to scan the blasted bottom surfaces, and rock surface roughness was quantitatively analyzed from the scanned profiles to apply to 3D FEM modelling. In addition, based on the 3D FEM model, a three-dimensional dynamic fracture process analysis (DFPA-3D) technique was applied to study on the shear behavior of the interface between blasted rock and concrete through direct shear tests, which was analyzed under constant normal load (CNL). The effects of normal stress and the joint roughness on shear failure behavior are also analyzed.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.4
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• pp.2006-2012
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• 2013

This case study deal with the investigation of various causes and analyses concerning the cases of the collapse of reinforced segmental retaining walls installed for newly constructing a peripheral road within the campus of ${\bigcirc}{\bigcirc}$ University located in Gyeonggi-do. As results of stability analyses and reviewing of design documents concerning collapsed reinforced segmental retaining walls, such a collapse appeared because of problems related to construction including poor-compacted backfill, the omission of the investigation on the bearing capacity, the length and space in the installation of reinforced materials, and drainage systems. Also, problems during diverse types of designing were confirmed involving the stability analysis of the entire slope stability to be considered during designing and failure in application of the proposed methods of FHWA or NCMA which are generally used for two-tier reinforced segmental retaining walls. In addition, based on these details of the stability assessment, the study proposed reinforcement solutions and construction methods for stabilizing reinforced segmental retaining walls to be reconstructed in the future.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.5
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• pp.372-378
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• 2016

A mound was constructed to install a caisson and sofa blocks underwater. The mound riprap, which were of uniform grade, size, shape, and specific gravity, formed the foundation for the support superstructure. Also, rubble leveling works were performed before installing structures such as caissons. In this study, underwater construction equipment was developed with a remotely controlled operating system and underwater environment monitoring system for unmanned underwater rubble leveling work. The performance of the developed equipment was verified using on-land and underwater tests. In addition to the performance verification, the construction process and economic efficiency of the equipment should be checked before applying it to the real construction field for commercial purposes. In this paper, a construction process using the developed equipment was proposed and compared with the existing rubble leveling method. The results demonstrated that the new construction method has higher economic efficiency and safety than the existing construction method.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.2
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• pp.1102-1108
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• 2014

This study was to compare the protection abilities of an SPF through ground or underwater casting. A mat of 1/10 scale was made and then mortar was placed on the ground and submerged conditions. A limit velocity of each mat was estimated with this experiment. As a result of the test, the mat failed because of the decrease of bearing power in the center of the waterway. On the one hand, the edge of the mat, where the velocity is slow, secures stability. The result of the limit velocity analysis suggests that a velocity of ground placement with 6.51m/s and underwater casting with 9.80m/s is the minimum to ensure stability. When SPF mat with a thickness of 0.50m is replaced with a concrete block, it is calculated to need a maximum thickness of 2.21m.

• Journal of the Korean Geotechnical Society
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• v.37 no.2
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• pp.33-48
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• 2021

The rock anchorage of a suspension bridge is an outstanding anchorage type from environmental and economical perspective, although it should be applied when the bearing foundation is fresh enough to resist large cable loads. In practice, geotechnical engineers have encountered difficulties in designing the anchorage structure due to the fact that the physical behaviors of rocks against cable loads have not yet been fully proved and its design method was not established yet. In this study, model tests and numerical studies were performed to evaluate the behavior of the rock anchorage system planned under hard rock layers in domestic islands, and results suggest that the shape of asymmetric rock wedges can resist the tension loads with self weight and shear resistance. Additionally, real scale trial tests were carried out to verify the accuracy of an inclined drilling penetrating hard rock layers to install tendon to the bearing plate.