• Title/Summary/Keyword: 지방 괴사

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Effect of Phenobarbital Pretreatment on the Hepatotoxicity of Carbon Tetrachloride in Rat (Phenobarbital 전처치가 사염화탄소 급성중독 흰쥐 간세포의 구조에 미치는 영향)

  • Byun, Young-Soo;Nam, Hae-Joo;Kim, Mi-Jin;Kim, Dong-Suk;Choi, Won-Hee;Lee, Tae-Sook
    • Journal of Yeungnam Medical Science
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    • v.9 no.1
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    • pp.137-148
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    • 1992
  • The purpose of this study was to evaluate the influence of phenobarbital(PB) on hepatotoxic effect of carbon tetrachloride($CCl_4$) which induces centrilobular necrosis in liver. Rats were injected intraperitoneally $CCl_4$ dissolved in olive oil by a dose of 0.4mg/kg. For change related to PB pretreatment, rats were injected $CCl_4$ 0.4mg/kg after PB pretreatment. The liver samples were taken in 6, 12, 24, 48, 72, and 120 hours after $CCl_4$ and/or FB injection. Extracted liver tissue was examined with light and electron microscopes. The results were sumarized as follows : 1. Light microscopic findings : In $CCl_4$ group, centrilobular necrosis developed from 6 hours after injection, was the most severe in 48 hours, and recovered after 72 hours. In addition to necrosis, fatty change and pale cell change were accompanied. In PB-$CCl_4$ group, necrosis occurred from 6 hours after $CCl_4$ injection and continued to 72 hours, and the degree of necrosis was more severe than that of $CCl_4$ group and pale cell change was decreased. 2. Electron microscopic findings: In $CCl_4$ group, the early principal change was clumping and vesicular dilatation of endoplasmic reticulum. In PB-$CCl_4$ group, the degenerative change of endoplasmic reticulum was aggrevated and the mitochondria also revealed severe degenerative change. According to the results, it was revealed that $CCl_4$ hepatotoxicity primarily began with the damage of endoplasimic reticulum, then damage of other cell organelles and cell necrosis followed, and these cytotoxic effects were aggrevated by PB pretreatment.

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Ultrastructural and Histochemical Study on the Epithelia of Digestive Tract of a Korean Slug, Incilaria fruhstorferi (한국산 산민달팽이 ( Incilaria fruhstorferi ) 소화관 상피조직의 미세구조 및 조직화학적 연구)

  • 이정찬;장남섭;한종민
    • The Korean Journal of Malacology
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    • v.13 no.2
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    • pp.143-160
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    • 1997
  • 한국산 산민달팽이 Incilariafruhstorferi의 소화관을 부위별로 관찰하고, 소화관 상피조직을 구성하는 세포의 종류와 분포수 그리고 분비되는 과립 등을 미세구조적, 조직화학적으로 관찰하고 그 결과를 보고하고자 한다. 산민달팽이의 소화관은 식도, 위, 장, 직장 등으로 구성되어있으며, 식도는 다시 전식도, 소낭, 후식도로 나누어지고, 장은 전장과 중장, 후장 등으로 구분되었다. 소화관을 부위별롸 관찰한 결과 섬모원주상피세포 2종, 녹색과립세포 3종, 청색과립세포, 점액세포, 투명세포, 망상형세포 그리고 괴사형세포 등 모두 10종류가 확인되었다. 섬모원주세포는 A형과 B형 등 두 종으로 나뉘며, A형은 세포의 상단 자유면에 섬모와 미세융모가 밀생된데 비해, B형은 전자밀도가 높아서 검게 보이고, 장과 직장에서만 관찰되는 특징을 보였다. 섬모의 구조는 9 x 2 + 2 axoneme이었다. 녹색과립세포는 미세구조에 의해 A형, B형 그리고 C형등 3종으로 나누어지며, 소낭과 후식도, 위, 직장에서 주로 관찰되었다. A형과 B형은 Sudan black에 양성인 지방과립(1.36 x 1.6$\mu\textrm{m}$)만을 포함하고 있는데비해 C형은 글리코겐 과립도 포함하고 있었다. 청색과립세포는 10종류의 세포 중 가장 키가 크고 (35$\mu\textrm{m}$), Millon반응에 양성을 보이는 둥근과립(직경, 1.3$\mu\textrm{m}$)을 포함하고 있는데 과립들은 단백질성으로 확인되었다. 이들은 중장에서만 관찰되었다. 장과 직장에서 주로 많이 관찰되는 점액세포는 세포질 속에 전자밀도가 낮은 투명한 과립과 전자밀도가 높아서 검게 보이는 둥근과립(크기, 1.33 x 0.89$\mu\textrm{m}$)들을 포함하고 있었는데, 이들은 미성숙 시기에는 투명과립(직경, 2.66$\mu\textrm{m}$)으로 관찰되었다. PAS-alcian blue(pH 2.5)반응에서 투명과립은 alcianophillia로 나타난 반면, 검은 과립은 PAS에 양성반을을 보이며, 각각 산성점액과 중성점액과립으로 확인되었다. 투명세포는 광학현미경 관찰에서 A형과 B형으로 구분되었으나, A형은 전자현미경 관찰에서 신경내분비세포로 확인된 반며, B형은 지방과립을 소지한 지방저장세포로 각각 확인되었다. 신경내분비세포가 소지한 과립의 크기는 0.16$\mu\textrm{m}$ 정도였다. 망상형세포는 주로 위에서 관찰되는 형태가 불규칙한 작은 세포로서 세포질에 비해 큰 핵을 소지하고 있었다. 소량의 세포질 돌기에는 사립체와 과립성소포체를 포함하고 있다. 괴사형세포는 후장과 직장에서 주로 많이 관찰되고, 점액세포의 점액과립이 분비된 후, 붕괴되는 과정에서 형성된 것으로 확인되었다.

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Primary Cardiac Lipoma Combined with Chest Pain Like Angina Pectoris (협심증 증상을 동반한 심낭내 원발성 지방종)

  • Kim Jung-Tae;Oh Tae-Yoon;Chang Woon-Ha
    • Journal of Chest Surgery
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    • v.39 no.4 s.261
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    • pp.320-322
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    • 2006
  • A 60-year-old male was admitted because of dyspnea and angina like chest pain. Noninvasive findings showed a mass at the posterior aspect of the left atrium. The mass was removed under cardiopulmonary bypass. An encapsulated adipose mass, which originated from the left atrium without any invasion to the pericardium, was completely excised. Histological examination showed mature adipose tissue with partial necrosis, confirming the diagnosis of lipoma. We report a rare of case of massive left atrial lipoma occupying the pericardial space.

Avascular Necrosis of Femoral Head on Bone Scan (대퇴골두 무혈성 괴사의 뼈스캔상의 병기)

  • Yang, Hyung-In;Kim, Eui-Jong;Kim, Deog-Yoon;Ryu, Kyung-Nam;Cho, Kyung-Sam
    • The Korean Journal of Nuclear Medicine
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    • v.28 no.2
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    • pp.206-213
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    • 1994
  • We studied 90 patients(179 femoral heads) with avascular necrosis of femoral head, who had been performed X-ray, bone scan and MRI to compare of the findings of AVN on bone scan between each other, retrospectively. The patients were 82 males and 9 females, their mean age was 45 years. Radiographic stages were classified by Steinberg modification, radionuclide stages were classified as followed; stage o(or type 0) : normal, stage 1 : faint ring like uptake around the femoral head, stage 2: intense ring like uptake, stage 3: irregular increased uptake with central photon defect, stage 4 : Intense diffuse increased uptake at femoral head and stage 5 : hip joint deformity with relatively mild increased uptake. The findings of MRI were classified according to extent, location, early or advanced lesion, signal intensity of the lesion and joint effusion. 156(87%) of 179 femoral heads had avascular necrosis, 68(75.5%) of 90 patients had bilateral AVN, 35 femoral heads had early stage and 120 had advanced stage. The detection rate of AVN by X-ray and bone scan were 85% (134), 91.6% (143), respectively. Early AVN with atypical types of bone scan showed larger extent, moderate to large amount of joint effusion, soft tissue hypertrophy within joint, and secondary degenerative changes. Bone scan had relatively high detection rate in the diagnosis of AVN of femoral head, and demonstrated various types depending on the disease stage.

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Triglycerides increase mRNA Expression of Pro-inflammatory Cytokines Via the iNOS in Jurkat T lymphocyte and U937 Monocyte Cell Lines (Jurkat T 림프구와 U937 단핵구에서 중성지방 처리 시 iNOS를 통한 염증성 사이토카인의 mRNA 발현 증가)

  • Chang, Jeong Hyun
    • Journal of the Korean Society of Radiology
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    • v.13 no.1
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    • pp.133-140
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    • 2019
  • Triglycerides (TG) are one of the triggers of chronic inflammatory lesions in the blood vessels. In the key factors in the development of inflammatory diseases, Pro-inflammatory cytokines such as tumor necrosis factor-alpha $(TNF-){\alpha}$ and interleukin-1 beta ($IL-1{\beta}$) contribute to the development of inflammatory lesions by recruiting other immune cells in the inflamed area or causing cell necrotic death. In this study, I investigated the effect of Jurkat T lymphocytes and U937 monocytes involved in vascular inflammation development on the expression of $TNF-{\alpha}$ and $IL-1{\beta}$ on exposure to TGs. In Jurkat cells, mRNA expression of $TNF-{\alpha}$ is increased by exposure to TGs. However, the expression levels of $TNF-{\alpha}$ and $IL-1{\beta}$ were increased by TGs in U937 cells. To investigate whether inducible nitric oxide synthase (iNOS) is involved in the increase of expression of $TNF-{\alpha}$ and $IL-1{\beta}$ by TGs, treatment of W1400 (an iNOS inhibitor) resulted in recovery of expression level both $TNF-{\alpha}$ and $IL-1{\beta}$. Based on the present study, it was confirmed that the expression of $TNF-{\alpha}$ and $IL-1{\beta}$ in monocytes and T lymphocytes. This increased cytokines contribute to development of vascular inflammatory lesions. In addition, iNOS is involved in the increase of $TNF-{\alpha}$ and $IL-1{\beta}$ expression by TGs.

Liposclerosing Myxofibrous Tumor - A case report - (지방경화성 점액섬유종(Liposclerosing myxofibrous tumor) - 1예 보고 -)

  • Yoo, Chang-Young;Jung, Eun-Sun;Park, Gyeong-Sin;Lee, Youn-Soo;Jee, Won-Hee;Kang, Chang-Suk;Lee, Kyo-Young
    • The Journal of the Korean bone and joint tumor society
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    • v.12 no.2
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    • pp.126-130
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    • 2006
  • Liposclerosing myxofibrous tumor (LSMFT) is a fibro-osseous lesion of the bone with a marked predilection for the intertrochanteric region of the proximal femur. It is characterized by a complex mixture of histological elements including fibrous dysplasia-like features, myxofibrous tissue, lipomatous area, ischemic ossification, xanthoma cells and pseudo-Paget's bone. Though some consider LSMFT as a variant of the fibrous dysplasia, intraosseous lipoma, or other benign osseous lesions, recently LSMFT is emerged as a genuine clinicopathologic entity. We experienced a 48-year female patient with typical histologic and radiologic findings of LSMFT. It was located at the intertrochanteric area of the femur. Radiologically, the lesion was radiolucent and ground-glass appearance with sclerotic rim in the plain film and magnetic resonance imaging. Histologically, myxofibrous tissue, lipomatous area and fibrous dysplasia-like features were predominant findings.

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Effects of Apolipoprotein A-I on Apoptosis and Cytokine Production in Human Neutrophils (인간 호중구의 세포사멸과 시토카인 분비에 대한 아포지방단백 A-I의 영향)

  • Kang, Hyung-Kon;Choi, Jae-Hyung;Huh, Jae-Taeck
    • Journal of Life Science
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    • v.21 no.1
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    • pp.22-30
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    • 2011
  • Apolipoprotein A-I (apoA-I) has anti-inflammatory and anti-oxidative properties. This study was designed to investigate whether apoA-I affects apoptosis and cytokine production of human blood neutrophils in an in vitro culture system. Spontaneous apoptosis of neutrophils was significantly delayed by apoA-I. In addition, high density lipoprotein containing apoA-I also delayed apoptosis of neutrophils. Apoptosis of neutrophils was inhibited by anti-scavenger receptor type B-I antibodies. The amounts of interleukin-8, interferon (IFN)-inducible protein 10 (IP-10), and tumor necrosis factor-$\alpha$ (TNF-$\alpha$) in the supernatants of cultured neutrophils treated with apoA-I were significantly increased. Combined treatment of neutrophils with IFN-$\gamma$ and apoA-I produced higher amounts of IP-10 and TNF-$\alpha$ than did treatment with IFN-$\gamma$ or apoA-I alone. The present study reveals that apoA-I activates neutrophils to produce cytokines and delays spontaneous apoptosis of neutrophils. These findings suggest that apoA-I, although a well-known negative acute-phase protein, has a pro-inflammatory effect in neutrophils.

MRI Evaluation for the Histologic Components of Soft-tissue Tumors: Comparison of MEDIC and Fast SE T2-weighted Imaging (연조직종양의 조직 성분 평가를 위한 자기공명영상: MEDIC 과 지방억제 T2 영상의 비교)

  • Moon, Tae-Yong;Lee, In-Sook;Lee, Jun-Woo;Choi, Kyung-Un;Kim, Jeung-Il;Kim, E. Edmund
    • Investigative Magnetic Resonance Imaging
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    • v.12 no.1
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    • pp.1-7
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    • 2008
  • Purpose : To compare Multi Echo Data Image Combination (MEDIC) and fast SE T2-weighted images with fat saturation (T2FS) to suggest more accurate evaluation of the histologic components of soft-tissue tumors. Materials and Methods : The experimental group included 25 histologic tissues (5 vascular, 4 neural, 4 fibrous, 4 hypercellular, 2 hemorrhagic necroses, 2 cystic, 2 lipoid, 1 myxoid stroma, and 1 thrombus) in 10 patients who had pathologically confirmed schwannoma (n = 3), hemangioma (n = 2), lipoma (n = 1), angiokeratoma (n = 1), synovial sarcoma (n = 1), liposarcoma (n = 1), and malignant fibrous histiocytoma (n = 1). The inhomogeneity values were measured using the standard deviation value (SD) divided by the mean value as SD presents an error amount similar to that of imaging heterogeneity. Results : The inhomogeneity values of 25 histologic components were lower on MEDIC than those on T2FS (p < .001). Conclusion : We conclude that MEDIC is more accurate than T2FS for evaluating the tissue components of soft-tissue tumors using digitalized data because MEDIC images have far lower inhomogeneity.

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An Imported Case of Kala-azar in Korea (중동에서 유입된 Kala-azar 1예)

  • 지제근;송영기
    • Parasites, Hosts and Diseases
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    • v.21 no.1
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    • pp.87-94
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    • 1983
  • Kala-azar는 우리나라에는 분포하지 않는 것으로 인정되고 있으나 과거 중국에서 감염되어 국내에서 관찰된 보고가 있는 질병이다. 최근에 중동 지방에서 감염되어 귀국하고 발병하였다고 생각되는 예를 보고한다. 서울에 주소를 둔 26세의 한국인 남자가 1년간 사우디 아라비아에서 건설기능공으로 근무하고 1981년 9월 귀국하여 약 8개월 후인 1982년 5월 경부터 심한 피로감, 전신쇠약, 복부의 수물등을 느끼기 시작하였다. 환자가 국립서울병원을 거쳐 서울대학교병원에 1982년 9월 17일 입원할 당시에는 간과 비장이 모두 14cm정도 만져질 만큼 부어있었고, 심한 체중 감소(4개월간 14kg의 감소), 적혈구, 백혈구, 혈소판등 모든 혈구의 감소등이 관찰되었다. 간생검을 통해 amastigote가 관찰되고 이는 투사전자현미경을 통해서 전형적인 amastigote형으로 확인되었다. 환자는 국외에서 구한 약제인 sodium antimony gluconate(Pentostam )와 각종 보조요법에 의해 치료를 받았다. 퇴원 직전에 시행한 간생검에서 괴사된 간 조직이 섬유화로 대치된 것이 확인되었고 충체는 관찰되지 않았다. 이 Kala-azar 에는 1952년 만주에 유입되어 보고된 3예 이후로는 처음 관찰된 것으로 중동에서 유입된 것으로는 첫번째 보고례이다. 최근 급격히 늘어난 해외 인력진출을 감안하면 해당 지역의 열대 풍토병에 대한 다각적인 대책이 절실히 요청된다.

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Histopathologic Studies on Livers in Ducklings Administered Aflatoxin Produced by Korean Industrial Strain of Aspergillus flavus (오리병아리의 간장(肝臟)에서 한국산 Aflatoxin이 유발시킨 병변(病變)에 관한 병리조직학적연구(病理組織學的硏究))

  • Yoon, Hwa Joong
    • Korean Journal of Veterinary Research
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    • v.15 no.1
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    • pp.133-145
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    • 1975
  • 한국산 aflatoxin의 독성을 비교 규명하기 위하여 결정 aflatoxin, 사료배합 aflatoxin 및 표준 aflatoxin을 오리병아리에 각 1회씩 경구투며 또는 급식시켜 얻은 결과는 다음과 같다. 1. 각종 aflatoxin의 독성으로 인하여 유발된 공통된 주요 병리조직학적 병변은 출혈(出血), 간세포(肝細胞)의 괴사(壞死), 지방변성(脂肪變性) 및 담관세포(膽管細胞)의 증식(增殖) 등이였다. 2. 병변(病變)의 정도(程度)는 독소의 투여량과 경과시간에 따라 다양(多樣)하게 나타났다. 3. 한국산 aflatoxin의 독성은 표준 aflatoxin에 비해 약간 더 심한 병변(病變)을 나타냈으며, 한국산 aflatoxin에서도 결정 aflatoxin을 투여한 경우보다 사료에 배합하여 급식시킨 경우가 약간 더 심한 병변(病變)을 나타냈다. 4. 닭병아리의 간장에서 aflatoxin이 유발시킨 병리조직학적 소견보다 오리병아리의 간장에서의 경우가 그 정도에 있어 더욱 심하고 뚜렷하였다.

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