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http://dx.doi.org/10.5352/JLS.2011.21.1.22

Effects of Apolipoprotein A-I on Apoptosis and Cytokine Production in Human Neutrophils  

Kang, Hyung-Kon (Department of Neurosurgery, College of Medicine, Donga-A University)
Choi, Jae-Hyung (Department of Neurosurgery, College of Medicine, Donga-A University)
Huh, Jae-Taeck (Department of Neurosurgery, College of Medicine, Donga-A University)
Publication Information
Journal of Life Science / v.21, no.1, 2011 , pp. 22-30 More about this Journal
Abstract
Apolipoprotein A-I (apoA-I) has anti-inflammatory and anti-oxidative properties. This study was designed to investigate whether apoA-I affects apoptosis and cytokine production of human blood neutrophils in an in vitro culture system. Spontaneous apoptosis of neutrophils was significantly delayed by apoA-I. In addition, high density lipoprotein containing apoA-I also delayed apoptosis of neutrophils. Apoptosis of neutrophils was inhibited by anti-scavenger receptor type B-I antibodies. The amounts of interleukin-8, interferon (IFN)-inducible protein 10 (IP-10), and tumor necrosis factor-$\alpha$ (TNF-$\alpha$) in the supernatants of cultured neutrophils treated with apoA-I were significantly increased. Combined treatment of neutrophils with IFN-$\gamma$ and apoA-I produced higher amounts of IP-10 and TNF-$\alpha$ than did treatment with IFN-$\gamma$ or apoA-I alone. The present study reveals that apoA-I activates neutrophils to produce cytokines and delays spontaneous apoptosis of neutrophils. These findings suggest that apoA-I, although a well-known negative acute-phase protein, has a pro-inflammatory effect in neutrophils.
Keywords
Neutrophils; apoptosis; apolipoprotein A-I; tumor necrosis factor-$\alpha$ (TNF-$\alpha$);
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